WS-5 Extract of <i> Curcuma longa, Chaenomeles sinensis,</i> and <i> Zingiber officinale</i> Contains Anti-AChE Compounds and Improves <i>β</i> -Amyloid-Induced Memory Impairment in Mice

Kim, Ju Eun,Shrestha, Abinash Chandra,Kim, Hyo Shin,Ham, Ha Neul,Kim, Jun Hyeong,Kim, Yeong Jee,Noh, Yun Jeong,Kim, Su Jin,Kim, Dae Keun,Jo, Hyung Kwon,Kim, Dae Sung,Moon, Kwang Hyun,Lee, Jeong Ho,Jeo Hindawi 2019 Evidence-based Complementary and Alternative Medic Vol.2019 No.-

<P>Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (A<I>β</I>) as senile plaques associated with reactive astrocytes and microglial activation in the brain. The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against A<I>β</I>-induced AD in mice and to identify the extract's active constituents. In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE). Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented A<I>β</I> oligomerization via inhibition of A<I>β</I><SUB>1-42</SUB> aggregation. Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS). Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells. WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-<I>α</I> and IL-6. The memory impairment in mice with A<I>β</I>-induced AD was studied using the Morris water maze and passive avoidance test. Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain. The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced A<I>β</I> plaque accumulation in A<I>β</I>-induced AD. HPLC analysis identified the extract's active compounds that exert anti-AChE activity. In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.</P>

[PB-0087] Efficiency Analysis of Agrobacterium Transformation of Korean Wheat Varieties By GUS System

Ha Neul Lee(Ha Neul Lee),Geon Hee Lee(Geon Hee Lee),Jae Yoon Kim(Jae Yoon Kim) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

편측 요통을 호소하는 환자에 있어서 척추 주위 근육의 지방량과 통증과의 관계

김하늘 ( Ha Neul Kim ),김경훈 ( Kyoung Hun Kim ),김주원 ( Joo Won Kim ),진은석 ( Eun Seok Jin ),하인혁 ( In Hyuk Ha ),고동현 ( Dong Hyun Koh ),홍순성 ( Soon Sung Hong ),권혁준 ( Hyeok Joon Kwon ) 한방재활의학과학회 2009 한방재활의학과학회지 Vol.19 No.1

목적: 요통은 임상에서 가장 흔하게 접할 수 있는 질환이며, 동시에 통증으로 인한 근무 결손도나 생활 불편감이 높은 질병이다. 그래서, 많은 연구들이 요통을 일으키는 원인 혹은 악화시키는 유발 인자를 밝혀내려는데 초점이 맞추어져 있다. 이번 연구의 목적은 편측 요통을 호소하는 환자에 있어서 MRI에서 조사된 척추 주위 근육의 지방량과 통증과의 관계에 대해서 알아보는데 있다. 방법: 2007년 11월부터 2008년 4월까지 본원에 요통으로 내원한 환자를 대상으로, 편측 요통을 호소하며, 체질량 지수가 25kg/㎡ 이하이며, 만 20세이상 40세미만인 환자 24명을 대상으로 연구를 진행하였다. MRI에서 조사되어진 L4-5 디스크 레벨의 척추 주위 근육의 지방량을 객관적인 pseudocoloring technique을 이용해 측정하고, paired t-test로 결과를 분석하여 요통이 유발된 측과 지방량의 관계에 대해서 연구하였다. 결과: 요통이 있는 측의 지방량과 요통이 없는 측의 지방량을 측정하여 데이터를 분석하였다. 양측의 지방량과 통증의 유무에 대한 관계는 통계적으로 유의한 수치를 나타냈다(P <0.05). 요통이 있는 측과 없는 측과의 성별, 나이, BMI의 통계적인 차이는 없었으며, 지방량 측정시 남자들이 여자들에 비해 지방량이 더 높게 측정되었다(남자 8.5±5.1%, 여자 6.5±3.6%). 결론: 이 연구를 통해 척추 주위 근육의 지방량과 요통에 대한 상관성의 증거를 제시하였다. 잘 선별되어진 환자의 MRI를 통해 객관적인 방법으로 지방량을 체크를 하였으며, 추후 더 많은 개체수를 이용해 보다 더 정확한 관련성을 파악하고, 나아가 지방량에 따른 통증 강도의 상관성에 대해서 연구가 필요하리라 생각된다. Objectives: Low back pain(LBP) is a common disabling disease in clinical practice and loss of working hours due to this condition is huge. The aim of this study was to determine if there was an association between fat deposit of paraspinal muscles as observed on MRI scans in patients presenting with unilateral LBP. Methods: 24 patients who visiting our hospital with a clinical presentation of unilateral LBP were recruited to the study. Patients were between 20 and 30 years and had a history of unilateral LBP within 12 months. After MRI scaning, the images were saved in DICOM file format for Picture Archiving and Communication System(PACS). The percentage of fat infiltrated area was measured using a pseudocoloring technique. Data were analyzed comparing the fat deposits of the muscles on the symptomatic and asymptomatic sides. Paired t-test was used to find the difference between the measurements of fat tissue in individual patients. Results: The amount of fat in the symptomatic side was 7.6±4.51%, asymptomatic side was 6.7±4.29%. There were increases, statistically significant, in the fat changes of the paraspinal muscles at the L4-5 disc level(P <0.05). Also, men were likely than women to have more fat deposit in symptomatic side(men 8.5±5.1%, women 6.5±3.6%). Conclusions: The amount of fat in the symptomatic side shows significantly increased than asymptomatic side in the paraspinal muscles at the L4-5 disc level. It suggested that fat infiltration in the muscles associated with LBP. Further studies will be needed to confirm the relationship between the muscle fatty changes and LBP in the large sample size. In addition, the correlation of pain severity with fat infiltration needs to be addressed.

Effects of microstructure and intergranular glassy phases on thermal conductivity of silicon nitride

Kim, Jin-Myung,Ko, Sin-Il,Kim, Ha-Neul,Ko, Jae-Woong,Lee, Jae-Wook,Kim, Hai-Doo,Park, Young-Jo Elsevier 2017 CERAMICS INTERNATIONAL Vol.43 No.7

<P><B>Abstract</B></P> <P>In this study, the binary sintering additives Y<SUB>2</SUB>O<SUB>3</SUB>-Sc<SUB>2</SUB>O<SUB>3</SUB>, were first applied to the Si<SUB>3</SUB>N<SUB>4</SUB> system to investigate their effects on microstructure and thermal conductivity. The microstructure and thermal conductivity of both sintered silicon nitride (SSN) and sintered reaction-bonded silicon nitride (SRBSN) were found to be significantly dependent on the additive composition. Among various combinations of Y<SUB>2</SUB>O<SUB>3</SUB> and Sc<SUB>2</SUB>O<SUB>3</SUB>, 1mol% Y<SUB>2</SUB>O<SUB>3</SUB>−3mol% Sc<SUB>2</SUB>O<SUB>3</SUB> prominently enhanced thermal conductivity, and the enhancement could not be attributed to any difference in microstructure or lattice defects. TEM observation revealed that this composition was more liable to devitrify the glassy phase with a lower degree of stress accumulation, and to possibly produce a grain boundary that was cleaner or with a higher order of atomic arrangement. A microstructure model for thermal conductivity was proposed which took the thermal resistance of the grain boundaries into account. The grain boundary state exerted a remarkable influence on the thermal conductivity of fine microstructures, and the experimentally measured thermal conductivity values were consistent with those given by the proposed model.</P>

Two cases of phymatous rosacea successfully treated by surgical and non-surgical treatment

( Ha Neul Oh ),( Tae Jun Park ),( Bo Young Kim ),( Chang Min Kim ),( Il-hwan Kim ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

In addition to a primary feature of rosacea, phymatous rosacea may include skin thickening, irregular surface nodularities, and patulous follicles. There are several therapeutic options for phymatous rosacea, including isotretinoin, antibiotics and surgical treatments. However, Phymatous rosacea can be disfiguring and difficult to treat. Case 1. A 79-year-old man patient presented with erythematous large nodular masses on the nose. Under the impression of phymatous rosacea in the advanced stage, because the patient complained about breathing discomfort and cosmetic problems, surgical treatment was considered. Electrocautery was performed to correct the deformity of the nose. Two months later, the lesion was obviously decreased and the results were satisfactory both functionally and cosmetically. Case 2. A 27-year-old man patient, with a history of aplastic anemia, presented with pricky erythematous plaque on the nose. Under the impression of phymatous rosacea in the early stage, treatment was initiated with isotretinoin 20 mg daily. There was a slight improvement at the beginning of the treatment and the lesion was definitely improved from 9 months after the treatment. Currently, the lesion is under the same treatment protocol without any side effects. We herein report on two cases of successfully treated phymatous rosacea, emphasizing the importance of choosing the appropriate treatment modality.

Crystal structure and functional characterization of SF216 from <i>Shigella flexneri</i>

Kim, Ha-Neul,Seok, Seung-Hyeon,Lee, Yoo-Sup,Won, Hyung-Sik,Seo, Min-Duk Wiley (John WileySons) 2017 FEBS letters Vol.591 No.21

<P>Shigella flexneri is a Gram-negative anaerobic bacterium that causes highly infectious bacterial dysentery in humans. Here, we solved the crystal structure of SF216, a hypothetical protein from the S.flexneri 5a strain M90T, at 1.7 angstrom resolution. The crystal structure of SF216 represents a homotrimer stabilized by intersubunit interactions and ion-mediated electrostatic interactions. Each subunit consists of three beta-strands and five alpha-helices with the beta-beta-beta-alpha-alpha-alpha-alpha-alpha topology. Based on the structural information, we also demonstrate that SF216 shows weak ribonuclease activity by a fluorescence quenching assay. Furthermore, we identify potential druggable pockets (putative hot spots) on the surface of the SF216 structure by computational mapping.</P>

EGF-chitosan hydrogel for therapeutic endoscopy of GI ulcers

Ha Neul LEE,Min Kyoung KIM,Jin Hee MAENG,Byoung Wook BANG,Jungju KIM,Kyunghyun MIN,Yi Xian LEE,Don Haeng LEE,Su-Geun YANG 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.4

The catalytic role of additive components for the nitridation of silicon/additive mixture

KIM, Ha-Neul,PARK, Young-Jo,KIM, Jin-Myung,LEE, Jae-Wook,KO, Jae-Woong,KIM, Hai-Doo,LEE, Seung-Jun,BAEK, Seung-Su,SEO, Il-Sung Ceramic Society of Japan 2016 JOURNAL OF THE CERAMIC SOCIETY OF JAPAN - Vol.124 No.3

<P>The nitriding behavior of silicon/additives mixture with varied composition was investigated by focusing on the catalytic effect of sintering additives (Y2O3 and Al2O3) and diluents (Si3N4). While sintering additives have originally been mixed to form a liquid phase to promote both densification and grain growth during post-sintering, it was revealed that the addition of Y2O3 enhances the nitriding reaction by eliminating the SiO2 and Si3N4 layer on the surface of silicon particles. Further, a synergistic effect resulting in an increased nitridation was verified by concurrent addition of sintering additives and diluents. The contribution of each component of the compact to the promoted nitriding reaction was elucidated in quasi in-situ manner by the combination of phase analysis and thermogravimetric analysis. (C) 2016 The Ceramic Society of Japan. All rights reserved.</P>

Hepatoprotective Effect of Cordyceps militaris in ob/ob Mice

Ha-Neul Choi,Yang-Hee Jang,Ah-Rong Kim,Min-Jung Kang,Jung-In Kim 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

Triallyl Borate as an Effective Separator/Cathode Interphase Modifier for Lithium-ion Batteries

Ha Neul Kim,Hye Rim Lee,Taeeun Yim The Korean Electrochemical Society 2023 Journal of electrochemical science and technology Vol.14 No.3

Ni-rich layered oxides cathode has recently gained attention as an advanced cathode material due to their applicable energy density. However, as the Ni component in the layered site is increased, the high reactivity of Ni⁴⁺ results in parasitic reaction associated with decomposing electrolyte, which leads to a rapid decreasing the lifespan of the cell. The electrolyte additive triallyl borate (TAB) improves interfacial stability, leading to a stable cathode-electrolyte interphase (CEI) layer on the LNCM83 cathode. A multi-functionalized TAB additive can produce a uniformly distributed CEI layer via electrochemical oxidation, which implies an increase in long-term cycling performance. After 100 cycles at elevated temperature, the cell tested by 0.75 TAB retained 88.3% of its retention ratio, whereas the cell performed by TAB-free electrolyte retained 64.1% of its retention. Once the TAB additive formed CEI layers on the LNCM83 cathode, it inhibited the decomposition of carbonate-based solvents species in addition to the dissolution of transition metal components from the cathode. The addition of TAB to LNCM83 cathode material is believed to be a promising way to increase the electrochemical performance.