Detection of Individuals Restoring Fertility by DNA Fragment Converted into STS (sequence tagged site) in Red pepper

Lee,Jang-Soo,Lee,Keun-Hyang,Kim,Yeo-Song,Kang,Kwon-Kyoo,Nou,Ill-Sup,Hirata,Yutaka 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Co-segregation of male fertility with DNA markers selected by RAPD analysis as being potentially linked to the restorer gene (Rf) for Cytoplasmic male sterility (CMS) was analyzed using segregating F2 population. One RAPD marker directly linked to the Rf locus was identified. Amplification of OPT-02/570 using the STS primers generated a monomorphic band of each fertile plants randomly selected F2 progenies. From these results, this specific marker would be strongly linked to be restoring gene. The use of STS marker is effective in overcoming the reliability of the RAPD phenotype and improving their utility for MAS, co-dominant STS markers are especially very useful.

다중 속성의 가중치를 이용한 인터넷 채용 협상 시스템의 설계 및 구현

이근수,이근왕,이종희 안성산업대학교 2002 論文集 Vol.33 No.-

These day, Internet Recruiting needs the negotiation of recruiting items. This paper proposes multilateral negotiation that substitutes applicants and employers. Previous Negotiation Systems use the preference value of the multi-attribute and the sequential negotiation. But proposed Internet Recruiting Negotiation System(IRNS) uses the parallel negotiation of multi-attribute. The parallel negotiation supplies the multi-attribute negotiation including values of single-attribute and result of parallel negotiation. This paper shows that excellent result for effective negotiation using the weight strategy of multi-attribute for a matter of concern of users.

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

직업성 요통 환자의 작업복귀

이종민,김수근,전재용,권대영 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.2

목적: 직업성 요통 환자에서 작업복귀의 예측 지표와 관련 요인을 알아보고자 하였다. 대상 및 방법: 산업재해 종결 및 장애 판정을 위해 전기진단검사가 의뢰된 42명의 직업성 요통 환자들을 대상으로 연령, 성별, 결혼상태, 교육정도 등의 일반적 특성과 통증기간, 통증빈도, 시각상사척도(VAS), Oswestry 장애척도, 수술여부, 치료기간 등의 요통 관련 요인들을 조사하였고, 1년 이상 경과 후 작업복귀 여부를 조사하여 작업복귀군과 작업미복귀군간의 일반적 특성, 수술여부, 전기진단검사, 시각상사척도(VAS), Oswestry 장애척도 등을 비교 분석하였다. 작업복귀의 예측지표와 관련 요인을 알아보기 위하여 단변량 분석 결과 작업복귀에 통계적으로 유의했던 변수들과 유의하지는 않았으나 작업복귀와 관련이 높을 것으로 생각되는 변수들로 다변량 로지스틱 회귀분석을 시행하였다. 결과: 42명의 환자 중 1년 후 작업복귀를 한 환자는 21명(50.0%)이었고 단변량 분석에서 통증빈도, 시각상사척도(VAS), Oswestry 장애척도, 수술여부가 통계적으로 유의한 차이를 보였다. 작업복귀군의 시각상사척도(VAS)와 Oswestry 장애척도는 각각 평균 7.1±2.0, 67.4±11.5%로 미복귀군의 6.1±2.1, 46.3±18.9%에 비하여 높았고 Oswestry 장애척도는 통계적으로 유의한 차이를 보였다(p<0.05). 다변량 로지스틱 회귀분석에서 Oswestry 장애척도가 작업복귀에 통계적으로 유의하였고 교차비는 0.044(95% 신뢰구간: 0.04∼0.53)이었다. 결론: 산재종결 및 장애판정을 위해 전기진단검사가 의뢰된 직업성 요통 환자를 대상으로 작업복귀의 예측 지표와 관련 요인을 조사하였으며 Oswestry 장애척도가 가장 주요한 요인이었다. The objective of this study was to identify prognostic factors for the return-to-work in patients with occupational low back pain. Forty-two patients (male 40, female 2) who were referred to electrodiagnostic laboratory for disability evaluation, were recruited. General characteristics, pain-related characteristics including intensity, duration, frequency and operation, electrodiagnostic abnormality, visual analog scale (VAS), and Oswestry index were investigated. Patients were followed up after one year to determine whether they have returned to work and compared prognostic factors for return-to-work. Logistic regression analysis was done to ascertain the relative contribution of each predictor to outcome of return-to-work. Twenty-one patients (50.0%) returned to work after one year and twenty-two patients (52.4%) had electrodiagnostic abnormalites. The mean value of Oswestry disability index was significantly higher in no-return-to-work group in comparison with return-to-work group (67.4±11.5% and 46.3±18.9%, respectively)(p<0.01). VAS score was higher in no-return-to-work group in comparison with return-to-work group (7.1±2.0, 6.1±2.1, respectively) which does not reach statistical significance. In multivariate logistic regression. Oswestry disability index was significantly related to return-to-work and odds ratio was 0.044(95% confidence interval: 0.04∼0.526) in the Oswestry disability index. The results of this study suggest that Oswestry disability index is useful as a predictor of return-to-work in patients with occupational low back pain.

인간 재조합 인터루긴-32 면역조절작용에 대한 유세포 분석

이광수,김영관,채정일,심정현,김은미,강형식,김수현,윤도영,명평근 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organ available for human transplantation. however, it remains various hurdles for clinical xenotransplantation. In pig and mouse xenotransplantation, porcine xenograft evoke a strong cellular rejection response in immunocompetent host and grafts are destroyed within a week. This cellular immune response could involved both T cells and NK cells. A number of groups have shown that human NK cells can recognize and damage porcine endothelial cells. In addition, human T cells can respond to porcine endothelial cells through both direct and indirect mechanisms. Cellular rejection of porcine tissues requires T cells, particularly CD4^(+) cells. A new cytokine recombinant human interleukin-32α,β(IL-32α,β) has a role innate and acquired immune system. In order to investigate the role of recombinant mouse IL-18 and recombinant human IL-32α,β in xenograft rejection, we transplanted the PK(15) cells to C57BL/6 mice with or without intraperitoneal injection of recombinant mouse IL-18 or recombinant human IL-32 α,β. It was analyzed the population of NK cell, T cell and B cell in the C57BL/6 mice transplanted with PK(15) cells and recombinant mouse IL-18 or recombinant human IL-32α,β by flow cytometry analysis. As a result, lymph node and thymus of PK15/IL18, PK15/IL32α and PK15/IL32β injected group were increased to T cell activation population than normal injected groups. CD8^(+) T cells were decreased in lymph node of PK15/IL18, PK15/IL32α and PK15/IL32β injected groups. CD4^(+) T cells were increased in lymph node cell of PK15/IL32α and PK15/IL32β injected group and also, B cell population were increased in lymph node cell and spleen of PK15/IL18, PK15/IL32α and PK15/IL32β injected group. Therefore, we suggest that recombinant mouse IL-18 and recombinant human IL-32α,β suppress xenograft rejection in cellular xenotransplantation.

걷기운동이 비만여성의 체수분의 변화에 관한 연구

이근일,김갑수 龍仁大學校 體育科學硏究所 2002 體育科學硏究論叢 Vol.12 No.1

The thesis has calculated the average (M) and the average deviation (SD) before and after by method of exercises against each analyzing item, so it has inspected a difference between before and after by method of exercises through the pared t-test. The inspection against a difference between the methods of exercises was verified to make a use of the t-test of the independent sample. A change of the bodily constitutions has a significant difference of weight, amount of physical fat, rate of physical fat, rate of abdominal fat, physical water, BMI, BMR through inspection between before and after by exercise methods of one groups of 6.5㎞/h and another group of 5.5㎞/h. There is a statistically significant difference by exercise methods from one groups of 6.5㎞/h and another group of 5.5㎞/h as well as weight, amount of physical fat, BMI and BMR. While it was reduced in rate of physical fat, that of abdominal fat in difference by exercise methods., however, there was no significant difference observed between two groups This study found out to have a more positive effects on the bodily constitutions from 6.5㎞/h than 5.5㎞/h as the result of its performance, The program with the moving speed of walking exercise is very good for a fat woman as an exercise to get rid of fat trouble, as well as the walking exercise is lower than the other exercise in rate of abandonment of the exercise and it is easy for everybody to take exercise. A moving speed of walking exercise have on very good effect on the exerciser when it takes 6.5㎞/h, 50 minutes or more, every five times per week. We needs more study on another sex together with a weight exercise and so on., in order to get rid of the fat trouble.

한글과 日本神代文字 : -"한글은 世宗이전에도 있었다"는 주장과 관련하여- -In relation to allegation: There was Hangul before King Sejong-

李覲洙 弘益大學校 1984 弘大論叢 Vol.16 No.1

It is not authentic to allege that Hangul was invented 4200 years ago, at the time of Dangun Ⅲ in terms of Cultural, linguistic, and literary history. It is a fabrication to assert that Hangul was made in imitating Jindai. Jindai is a counterfeit fabrication by ultranationalists.

近代 韓國史學의 成長

李根洙 울산대학교 1978 연구논문집 Vol.9 No.2

近代韓國史學은 19세기말 20세기초의 우리나라가 근대로 移行하는 時期부터 비롯된다. 그러나 歷史意識面과 역사서술 方法의 측면에서 종래의 國史 硏究보다 크게 진보한 17·8세기 실학자의 국사 연구에서 이미 近代 韓國史學은 胎動하고 있다. 이러한 近代 韓國史學은 1880∼1910年代에 애국계몽史觀에 의한 學者에 의하여 애국정신 민족정신을 國史硏究에 의해 계발하려는 노력의 결과로 발달하기 시작한다. 그러나 우리나라에 대한 식민통치의 合理的인 근거를 우리나라 歷史에서 찾으려는 이른바 日帝 어용학자의 식민주의적 韓國史觀에 의해 近代韓國史學은 커다란 위기를 맞게된다. 우리나라 歷史의 타율성·정체성을 中心으로 하는 日人學者의 植民史觀을 비판·극복하는 과정에서 近代 韓國史學은 폭넓게 發展하여 1930·40年代에는 세 學派로 多樣하게 성장하게 되니 박은식·신채호를 계승한 民族主義史學과 社會經濟史學 實證主義 史學이 그것이다. 그리하여 解放후에는 이들 세 學派가 병진 보완하여 日帝 植民史觀은 완전히 극복되고 近代 韓國史學의 成長은 完結된다. The modern history of Korea dates from the time when Korea moved on to modernization around the end of the nineteenth century. But it already began budding in the studies of history by the seventeenth and eighteenth century scholars of Sil-Hak(practical learning), whose works were far more advanced from the aspects of historical sense and their methods of description than those done by their predecessors. Such study of modern Korean history began to develop as a result of endeavoring to cultivate nationalism and patriotic spirit on the part of the scholars who studied history from the viewpoint of patriotic enlightenment in the period between 1880 and 1910. However, it was brought to a crisis by the colonialistc view of history of the Japanese scholars in government pay who sought the rational ground of their colonial rule in the Korean history. In the course of critieizing and trying to overcome the colonial view of history of the Japanese based on the heteronomy and static state of Korean history, our study of modern history marked remarkable advance, resulting in the growth of three different schools: the school of nationalism which inherited the history of positivism. Since the liberation of Korea these three schools completed the growth of the study in modern Korean history, advancing side by side and complementing one another.

컴퓨터 시각 시스템에 있어서 제어기의 확장

李根壽,金三根 안성산업대학교 1990 論文集 Vol.22 No.-

수근관 증후군의 진단기준 : 일개 육류 가공업체 근로자의 수근관 증후군

이종민,김수근,김종민 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.3

Objectives : This study was conducted to determine the prevalence and status of work-related carpal tunnel syndrome in meat-processing workers and to assess the usefulness of diagnostic criteria using symptoms, physical findings and electrodiagnostic tests. Methods : The subjects were 34 female workers with average age of 43.1±8.5 years and clinical studies including history taking for symptoms, physical examination and electrodiagnostic tests were conducted. Results : Using a case definition of positive symptoms and electrodiagnostic abnormalities, 8 workers were diagnosed as carpal tunnel syndrome and the prevalence was 23.5%, 5 workers(14.7%) had symptoms but no electrodiagnostic abnormalities and 2 workers(5.9%) had only electrodiagnostic abnormalities. 19 workers (55.9%) had no symptoms and no electrodiagnostic abnormalities. There were no statistically significant differences in the prevalence of carpal tunnel syndrome between high repetitive work group and low repetitive work group among the workers. Age and daily working hours was related to the prevalence of carpal tunnel syndrome at 0.10 significant level(p-value=0.10) Conclusions : The combination of symptoms and night symptoms or symptoms and physical findings is a useful diagnostic criteria for screening of work-related carpal tunnel syndrome when electrodiagnostic test or a trained physician for physical examination are not available in that sensitivity, specificity, positive predictive value and negative predictive value were 0.63, 0.85, 0.56 and 0.88. respectively.