http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
영지(Ganoderma lucidum)균사체의 액체배양에 의한 세포외 수용성 다당류의 분획 및 항암활성
이신영,강태수,문순옥,류인덕,이명열 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
영지 균사체의 액체배양에 의하여 생산된 세포외 다당 시료를 분획하였고, 이들 각 분획에 대한 항암 활성을 균사체 유래 다당 분획들과 비교하면서 조사하였다. 배양여액으로부터 세포외 수용성 다당(BWS)을 얻었고, 이를 순차적으로 이온교환 및 겔 크로마토그라피 하여 BWS를 포함한 세포의 수용성 다당 분획, 총 5종을 얻었으며, 균사체 유래의 수용성 다당(MWS)에서도 세포외 다당과 동일한 방법으로 분획하여 총 5종의 분획을 얻어 대조구로 시용하였다. 세포외 다당 시료들의 항보체 활성(ITCH_50, %)은 15~30% 정도로 15~70%인 균사체 유래 다당 분획들의 값보다는 낮았으나, 이들 세포외 다당 분획들의 값보다는 낮았으나, 이들 세포외 다당 분획들중, BWS-DA와 BWS-DA-GI는 1000 ㎍/ml의 시료 농도에서 30% 정도의 매우 높은 활성을 나타내었다. MTT assay에서는 mouse leukemia L1210에 대하여 BWS-DN 및 MWS가 600㎍/ml의 농도에서 각각 86 및 89%의 높은 저해율을 나타내었으며, BWS-DA 및 MWS-DN의 경우는 3㎍/ml의 매우 낮은 농도에서도 32~35%의 저해율을 보였다. 또 human cell line인 COLO-205에 대해서는 BWS와 BWS-DA 및 MWS-DA가, 그리고 HL-60에 대해서는 BWS-DA와 BWS-DN 및 MWS-DN이 각각 300㎍/ml의 시료 농도에서 약 50%(IC_50)의 높은 저해능을 나타내었다. Exo-polysaccharide (BWS) obtained from submerged cultivation of Ganoderma lucidum mycelium was fractionated. Antitumor activity of their fractions was investigated in comparison with the mycelial polysaccharide fractions. Eight kinds (BWS-DN, BWS-DA, BWS-DN-GI, BWS-DA-GI, MWS-DN, MWS-DA, MWS-DN-GI and MWS-DA-GI) of polysaccharide fractions were obtained by DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel chromatography from BWS and MWS, which were isolated from culture fluid and mycelial cell, respectively. The anticomplementary activities (ITCH_50, %) of the exo-polysaccharide fractions showing 15% to 30% were lower than those of mycelial polysaccharide fractions showing 15% to 70%. The acidic fractions of BWS-DA and BWS-DA-GI fractionated from BWS, showed the highest activity of 30%. In the MTT assay, BWS-DN and MWS against mouse leukenia L1210 exhibited high inhibition ratio of 86 and 89%, respectively at the concentration of 600 ㎍/ml. High inhibition ratio of 50% (IC_50) was achieved for BWS, BWS-DA and MWS-DA fractions against human colon adenocarcinoma COLO-205 and for BWS-DN, and MWS-DN fractions against human leukemia HL-60 at the concentration of 300 ㎍/ml among the six polysaccharide fractions, respectively.
A Note on the harmonic properties about the analytic projective geometry
Shin,Deok Kang 관동대학교 1980 關大論文集 Vol.8 No.1
세 실수의 순서쌍을 도입하여 정의된 점과 직선의 동차좌표를 이용하여 조화열점의 정의를 해석적으로 정의하고 이 좌표를 써서 사영기하에 관한 여러가지 성질들을 해석적으로 증명할 수 있다. 여기서는 주로 조화열점에 관한 성질들을 해석적 방법을 이용하여 살펴보았다.
선형 삼각형 격자 근사법을 활용한 지상 레이저 스캐닝에 의한 구조물의 변형 계측기법
강덕신 ( Kang Deok Shin ),이홍민 ( Lee Hong Min ),박효선 ( Park Hyo Seon ) 한국구조물진단유지관리공학회 2007 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.11 No.2
Terrestrial laser scanning (TLS) delineates surface of every solid object precisely in three dimensions and is recently applied to various fields of geographic information system (GIS), natural science, civil engineering and so on. In the former research, estimation model on stress of structures using TLS is proposed. The method generated deformed shape of the structure appling grid subdivision and average coordinates of points in each subdivision area. However it was not sufficient for structural health monitoring because of errors originated from 3-D data of TLS. Therefore, in this paper, the linear triangle grid fitting method as an advanced approach is suggested for processing the 3-D data from TLS and deflections measured from TLS system are compared with deflections directly measured from linear variable displacement transducers (LVDT).
Schisandrae Fructus Reduces Symptoms of 4-Vinylcyclohexene Diepoxide-Induced Ovarian Failure in Mice
Shin, Dasom,Ha, Jinhee,Hong, Seong Bin,Kang, Geun-Hyung,Hwang, Deok-Sang,Bae, Hyunsu Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-
<P>Menopause is associated with a decrease in the level of sex hormones such as ovarian estradiol and progesterone and can cause various symptoms such as depression, hot flash, fatigue, heart palpitations, and headache. Furthermore, there is a risk of developing complications such as osteoporosis, cardiovascular diseases, Alzheimer's disease, and ovarian cancer. Schisandrae Fructus (SF) is widely used in Korean medicine as a cure for such complications. This study was conducted to evaluate the therapeutic effects of SF against menopause symptoms associated with follicle depletion caused by the industrial chemical 4-vinylcyclohexene diepoxide (VCD) in mice. VCD directly targets the preantral follicles. Mice were injected with VCD (160 mg/kg intraperitoneally) daily for 15 days and then with SF dosage 3 times/week for six weeks. To evaluate the effects of SF, body weight, tail skin temperature, uterine weight, lipid profile, and osteocalcin levels were measured. A decrease in body weight and tail skin temperature and an increase in uterine weight were observed upon SF treatment. Moreover, SF treatment significantly decreased total cholesterol, triglyceride, osteocalcin, and low-density lipoprotein levels and low-density/high-density lipoprotein ratio. These results suggest the potential use of SF in the treatment of menopausal symptoms in women.</P>
( Deok-bog Moon ),( Sung-gyu Lee ),( Shin Hwang ),( Ki-hun Kim ),( Chul-soo Ahn ),( Tae-yong Ha ),( Gi-won Song ),( Dong-hwan Jung ),( Gil-chun Park ),( Woo-hyung Kang ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: The large volume of adult living donor liver transplantations (ALDLTs) at our center affords a unique opportunity to examine the impact of acute-on-chronic liver failure (ACLF) among high-MELD score patients. Methods: From February 1998 to March 2010, 1958 cirrhotic recipients were analyzed to study the relationship between MELD scores and ALDLT outcomes. A total of 327 high-MELD score recipients were categorized into ACLF and non-ACLF groups, and their outcomes were compared. Results: The five-year graft and patient survival in the high MELD group were 75.2% and 76.4%, respectively, which were significantly worse than the low and intermediate MELD groups. The presence of ACLF associated with higher MELD scores appeared to be the dominant factor responsible for the inferior results of patients with in MELD score of 30~34 points. The five-year graft survivals in the ACLF group was 70.5% and in the non-ACLF group 81.0% (P = 0.035). Conclusions: Therefore, ALDLT should be performed as soon as possible in high-MELD score patients prior to ACLF development. Moreover, ACLF patients should be separately categorized when analyzing the outcomes of ALDLT. ALDLT for ACLF patients should not be discouraged because favorable outcomes can be expected through timely ALDLT and comprehensive management.
( Shin Hwang ),( Su-min Ha ),( Chul-soo Ahn ),( Ki-hun Kim ),( Deok-bog Moon ),( Tae-yong Ha ),( Gi-won Song ),( Dong-hwan Jung ),( Gil-chun Park ),( Hwi-dong Cho ),( Jae-hyun Kwon ),( Sang-hyun Kang 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: This study aimed to assess patterns of hepatocellular carcinoma (HCC) recurrence after liver transplantation (LT) and to establish long-term surveillance protocols for late HCC recurrence. Methods: The 232 LT recipients experiencing subsequent HCC recurrence were categorized as Group 1, early recurrence (within 1 year of LT; n=117); Group 2, late recurrence (occurring in years 2-5; n=93); and Group 3, very late recurrence (after year 5; n=22). Results: Recurrence was detected by only elevated tumor marker levels in 11.1%, 30.1%, and 45.5% of patients in Groups 1, 2, and 3, respectively (P<0.001). The proportion of intrahepatic and extrahepatic metastases was similar in all three groups. Common sites of extrahepatic metastasis were the lung and bone; these were also similar across the three groups. Overall post-recurrence patient survival rates were 60.2% at 1 year, 28.2% at 3 years, 20.5% at 5 years, and 7.0% at 10 years. Median post-recurrence survival periods were 10.2, 23.8, and 37.0 months in Groups 1, 2, and 3, respectively. Conclusions: While the pattern of HCC recurrence was similar regardless of time of recurrence, post-recurrence survival was significantly longer in patients with later recurrence. Long-term surveillance for HCC recurrence beyond 5 years post-LT is recommended.
( Shin Hwang Sang ),( Hyun Kang Young ),( Joo Lee Ki ),( Hun Kim Chul ),( Soo Ahn Deok ),( Bog Moon Tae ),( Yong Ha Gi ),( Won Song Dong ),( Hwan Jung Sung ),( Gyu Lee ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Several important changes were made to the 8th edition of American Joint Commission on Cancer (AJCC) tumor staging system for intrahepatic cholangiocarcinoma (ICC). We assessed the prognostic impact of this new tumor staging system compared to the 7th edition. Methods: A retrospective single-institution study was performed with 626 patients who underwent R0 resection for ICC over 20-year period. Results: Anatomical resection and concurrent bile duct resection were performed in 571 (91.2%) and 62 (9.9%) patients, respectively. Cumulative tumor recurrence and patient survival rates were 40.6% and 73.3% at 1 year; 66.7% and 43.8% at 3 years; 73.6% and 30.4% at 5 years; and 74.4% and 20.3% at 10 years, respectively. Independent prognostic factors for tumor recurrence and patient survival were multiple tumors, CA 19-9 >200 U/mL, tumor size >5 cm, direct invasion to extrahepatic structure, and lymph node metastasis. For TNM stages in the 7th versus the 8th editions, C-index was 0.615 and 0.625 for tumor recurrence and 0.626 and 0.628 for patient survival, respectively. Conclusions: The 8th edition AJCC appears to provide high prognostic contrast for T stage categories, except for T3. However, overall prognostic performance of the 8th edition was not markedly improved over the 7th edition.