대학생의 성교육 효과측정을 위한 기초 연구

장순복,최연순,강희선,박소미,Chang, Soon-Bok,Choi, Yun-Soon,Kang, Hee-Sun,Park, So-Mi 대한간호협회 1997 대한간호 Vol.36 No.2

This study was performed to provide preliminary data for the development of a useful instrument to measure the effect of sex education. The study was conducted with the voluntary participation of 155 college students enrolled in the course "Sexuality and Relationship" at Y university in Seoul. At the end of that course, they were asked to write freely about the change they had experienced on sexuality. All meaningful statements were elicited and classified into 7 categories; "Change of Knowledge", "Enlightenment", "Change of Attitude on Sexuality", "Buildup of Ability", "Buildup of. Identity", "Change of Emotion", "Change of Behavior:' There were significant changes in the areas of knowledge(28. 6%), enlightenment(27.4%), and attitudes(20.3%) about sexuality among these 7 categories. The Change of Knowledge category consists of 3 areas: "concretion of knowledge", "increase of information", and "correction of misunderstanding." In the category of Enlightenment, total 12 areas are included: "sex role", "erception of lack of knowledge", "importance of family", "life plan", "parent role", "value of life", "equality", "sexual autonomy", "importance of sexuality", "freedom of sexuality", "perception of sexual problem", and "meaning of love." The Attitude Change category consists of 8 areas. These are "being natural", "being progressive", "being sensitive", "being truthful", "being expressive", "being cautious", "being responsible", and "being confident". The category of Buildup of Ability includes 4 areas: "problem solving", "sex education", "relationship", and "communication". The category of Buildup of Identity includes "sexual identity", and "value of sexuality". The Emotional Change category includes 3 areas: "positive feeling", "negative feeling", and "breaking from negative feeling on sexuality". The Behavior Change category includes "sex-related behavioral change". In conclusion, up to now most researches on sex education effect measures only changes of knowledge, attitude, and behavior. But we believe the changes in "Enlightenment", "Buildup of Ability", "Buildup of Identity", "Change of Emotion", should be included in addition to knowledge, attitude, and behavior in the development of an instrument to measure the sex education effects. And the effect of sex education should measure the degree of learning of autonomy, enlightenment, and ability of behavior and so on rather than studying the simple changes in sexuality.enment", "Buildup of Ability", "Buildup of Identity", "Change of Emotion", should be included in addition to knowledge, attitude, and behavior in the development of an instrument to measure the sex education effects. And the effect of sex education should measure the degree of learning of autonomy, enlightenment, and ability of behavior and so on rather than studying the simple changes in sexuality.ng the simple changes in sexuality.

Gonadal Development, Spawning and Plasma Sex Steroid Levels of the Indoor Cultured Grunt, Hapalogenys nitens

Hee Woong Kang,Jae-Kwon Cho,Maeng-Hyun Son,Jong Youn Park,Chang Gi Hong,Jae Seung Chung,Ee-Yung Chung 한국발생생물학회 2015 발생과 생식 Vol.19 No.1

The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-17β of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-17β were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.

Gonadal Development, Spawning and Plasma Sex Steroid Levels of the Indoor Cultured Grunt, Hapalogenys nitens

Kang, Hee Woong,Cho, Jae-Kwon,Son, Maeng-Hyun,Park, Jong Youn,Hong, Chang Gi,Chung, Jae Seung,Chung, Ee-Yung The Korean Society of Developmental Biology 2015 발생과 생식 Vol.19 No.1

The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-$17{\beta}$ of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-$17{\beta}$ were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.

Investigation of channel mobility in AlGaN/GaN high-electron-mobility transistors

Chang, Sung-Jae,Kang, Hee-Sung,Lee, Jae-Hoon,Yang, Jie,Bhuiyan, Maruf,Jo, Young-Woo,Cui, Sharon,Lee, Jung-Hee,Ma, Tso-Ping Institute of Pure and Applied Physics 2016 Japanese Journal of Applied Physics Vol.55 No.4

<P>We have investigated the channel mobility in the gated region of a set of high-quality AlGaN/GaN high-electron-mobility transistors (HEMTs). The resistances in the contact, access, and gated regions were extracted from straightforward I-D(V-G) measurements on sets of HEMTs with four different gate-to-drain distances. By correcting for the effects of the contact and access resistances, much more accurate effective mobility curves in the gated region, compared to those reported in the past, have been obtained. The maximum effective mobility in that region has been found to be 1100cm(2)V(-1) s(-1) at carrier density n(s) = 7 x 10(12)cm(-2) at room temperature. We have extracted the mobility curves in a wide range of temperatures (80 to 520 K) and carrier concentrations (up to 1.3 x 10(13)cm(-2)). Our systematic measurements have revealed various dominant scattering mechanisms as the temperature and carrier concentration change. (C) 2016 The Japan Society of Applied Physics</P>

배지의 무기염류와 sucrose 농도가 감자의 기내 shoot 증식과 기내경 삽목묘의 생존율에 미치는 영향

김희곤,임순희,안장순,송창길,박미경,강봉균 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

감자의 virus-free 기내경 삽목묘의 효율적인 대량 생산 체계의 확립을 위하여 생장점 배양으로 얻어진 감자의 기내경의 증식과 기내경의 순화삽목을 실시한 결과는 다음과 같다. 초대배양에서는 염류농도와 sucrose 농도가 높을수록 양호하였다. 즉, MS나 2MS에 sucrose를 6% 첨가한 배지에서 튼튼한 기내경이 생산되었다. 계대배양에서는 sucrose 농도가 높을수록 줄기의 길이는 단축되고 굵기는 커졌으며 생체중은 염류농도와 관계없이 3%의 sucrose가 첨가된 배지에서 무거웠다. 이들 유식물체의 경절을 삽목한 결과 계대배양 배지의 sucrose농도가 6%인 배지에서 생존율이 높은 경향이었다. In order to establish a mass propagation system of potato planting material, in vitro multiplication of virus-free shoot originated from meristem culture and sucrose concentrations on survival of cutting of the in vitro stem tried with various media containing different levels of MS salts(0.5x, 1x and 2x) and sucrose(1.0, 3.0, 6.0%), and the effects of MS salts elements were evaluated. Higher level of sucrose in the initial culture media favored the shoot growth, that is, both of 1MS and 2MS media containing 6.0% sugar produced stouter shoots. Higher level of sucrose in the subculture media also favored the development of compact stout shoots, but biomass production in terms of fresh weight was high on the media containing 3.0%sucrose, regardless of MS salts concentration. The survival rate of cuttings made of the in vitro stem segments tended to be higher in those obtained from subculture media containg 6.0%sucrose.

Clinical Experiences and Usefulness of Cervical Posterior Stabilization with Polyaxial Screw-Rod System

Hwang, In-Chang,Kang, Dong-Ho,Han, Jong-Woo,Park, In-Sung,Lee, Chul-Hee,Park, Sun-Young The Korean Neurosurgical Society 2007 Journal of Korean neurosurgical society Vol.42 No.4

Objective : The objective of this study is to investigate the safety, surgical efficacy, and advantages of a polyaxial screw-rod system for posterior occipitocervicothoracic arthrodesis. Methods : Charts and radiographs of 32 patients who underwent posterior cervical fixation between October 2004 and February 2006 were retrospectively reviewed. Posterior cervical polyaxial screw-rod fixation was applied on the cervical spine and/or upper thoracic spine. The surgical indication was fracture or dislocation in 18, C1-2 ligamentous injury with trauma in 5, atlantoaxial instability by rheumatoid arthritis (RA) or diffuse idiopathic skeletal hyperostosis (DISH) in 4, cervical spondylosis with myelopathy in 4, and spinal metastatic tumor in 1. The patients were followed up and evaluated based on their clinical status and radiographs at 1, 3, 6 months and 1 year after surgery. Results : A total of 189 screws were implanted in 32 patients. Fixation was carried out over an average of 3.3 spinal segment (range, 2 to 7). The mean follow-up interval was 20.2 months. This system allowed for screw placement in the occiput, C1 lateral mass, C2 pars, C3-7 lateral masses, as well as the lower cervical and upper thoracic pedicles. Satisfactory bony fusion and reduction were achieved and confirmed in postoperative flexion-extension lateral radiographs and computed tomography (CT) scans in all cases. Revision surgery was required in two cases due to deep wound infection. One case needed a skin graft due to necrotic change. There was one case of kyphotic change due to adjacent segmental degeneration. There were no other complications, such as cord or vertebral artery injury, cerebrospinal fluid leak, screw malposition or back-out, or implant failure, and there were no cases of postoperative radiculopathy due to foraminal stenosis. Conclusion : Posterior cervical stabilization with a polyaxial screw-rod system is a safe and reliable technique that appears to offer several advantages over existing methods. Further biomechanical testings and clinical experiences are needed in order to determine the true benefits of this procedure.

소의 대동맥 내피세포에서 산화저밀도 지단백에 의한 아포프토시스에 백일해 독소 및 콜레라 독소가 미치는 영향

손호영(Ho Young Son),강성구(Sung Ku Kang),강무일(Moo Il Kang),차봉연(Bong Youn Cha),이광우(Kwang Woo Lee),장상아(Sang Ah Chang),최윤희(Yoon Hee Choi),문성대(Sung Dae Moon),안유배(Yoo Bae Ahn),손현식(Hyun Sik Son),윤건호(Kun Ho Yoon) 대한내과학회 1999 대한내과학회지 Vol.56 No.5

N/A Objectives : Oxidized LDL is thought to play a key role in atherogenesis. Among their wide variety of biological properties, oxidized LDL injures the endothelium as an early event of atherogenesis. However, the mechanisms by which oxidized LDL injures endothelial cells are not definitely known. In order to evaluate the involvement of GTP-binding protein on the mechanism of apoptosis, we studied the effects of pertussis and cholera toxin on oxidized LDL-induced apoptosis in bovine aortic endothelial cells(BAECs). Methods : Oxidized LDL elicited apoptosis in cultured BAECs as shown by characteristic morphological and biochemical changes. Chromatin condensation and nucleus fragmentation were visualized by using fluorescence microscopy of intact cells staining by acridine orange/ ethidium bromide. DNA fragmentation was quantified by an ELISA with specific antibody for bromodeoxyuridine- labelled DNA fragments and confirmed with DNA ladder formation. Results : Studies using a combination of bacterial toxins which change the function of GTP-binding protein suggest that oxidized LDL-induced apoptosis was regulated by GTP-binding protein. Oxidized LDL-induced apoptosis was not changed by pretreatment of BAECs with pertussis toxin. In contrast, pretreatment with cholera toxin completely prevented the oxidized LDL- induced apoptosis. Conclusion : These results show that oxidized LDL induces apoptosis of BAECs and suggest that cholera toxin-sensitive G-proteins are involved in signal transduction of oxidized LDL-induced apoptosis of BAEC.

자궁내막기질인 Type I Collagen을 이용한 생쥐 배아의 착상에 관한 연구

김정훈(Chung Hoon Kim),채희동(Hee Dong Chae),강병문(Byung Moon Kang),장윤석(Yoon Seok Chang),강은희(Eun Hee Kang),전용필(Yong Pil Cheon),박견(Kyun Park) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.1

목적: Type I collagen을 이용하여 3차원적 배양체계와 유사한 구조적인 환경을 조성하여 생쥐 배아의 착상전후의 상태를 보기 위한 연구를 중심으로 하였다. 연구방법: 포배단계의 배를 획득하기 위해서 자궁과 수란관의 일부를 적출하고 배양액으로 관류하여 배를 획득한 후 실험에 사용하였다. 멸균된 centrifuge용 시험관에 collagen이 polymerization되게 하였다. 배아는 착상과정의 비교를 위하여 type I collagen군(실험군)과 대조군으로 나누었으며, 대조군에서는 type I collagen 없이 배양액만 사용하여 배아를 배양하였다. 배의 성장과 분화는 24시간 단위로 해부현미경하에서 관찰하였다. 결과: 배양 제 3일에 배아의 부착율은 차이가 없었으나, 배아의 크기는 대조군에 비하여 실험군에서 뚜렸하게 컸다. 배아의 성장이 대조군에서 배양용기의 표면을 따라 수평적으로 진행되는 것과는 달리 실험군에서는 구형을 이루며 입체적으로 성장하기 때문에 색깔이 훨씬 진하게 나타났다. 배아의 발달단계를 관찰한 결과 배양 제 4일과 5일에는 실험군에서 대조군에 비하여 유의하게 발달단계가 높았다. 결론: Type I collagen을 사용한 본 연구는 착상연구의 모델로서 실험적으로 원하는 환경을 설정할 수 있고 관찰이 용이한 장점을 보여주었으나, 생체의 자궁내막세포와 같은 dynamic milieu가 결여되어 있어, 실제의 자궁내막과 유사한 체계를 이루는 배양체계를 어느 정도 수준까지 완성하려면 지속적인 연구가 필요할 것으로 사료된다. Objectives: To examine the in vitro interactions of blastocyst attachment using type I collagen. Materials and methods: ICR mice were used and follicular growth was stimulated by pregnant mare serum gonadotropin and human chorionic gonadotropin. On day 4 of pregnancy, the uteri were removed and blastocysts were flushed. Mixtures of 1mL sterile water, 0.5mL DMEM, 2mL type collagen solution and 0.5mL 0.1M NaOH were prepared and transferred to an incubator where the collagen solution polymerized. Blastocysts were transferred to dishes previously coated with type I collagen. CMRL 1066 was used as the basic culture medium. It was supplemented with 1mM glutamine and 1mM sodium pyruvate plus 50 IU/ml penicillin and 50 mg/ml streptomycin. During the first 4 days the culture medium was supplemented with 20% fetal calf serum and thereafter with 20% heat inactivated human cord serum. All blastocysts were initially cultured for 2 days without media change. After 2 days, fresh medium was renewed daily. The stages of embryo growth were examined and recorded everyday under a dissecting microscope and classified according to the standard in vivo criteria set forth by Witschi. Results: By 48h, nearly all blastocysts had attached to the surface of collagen pad. Following adhesion to the collagen pad, the blastocysts maintained their 3-dimensional integrity in contrast to control. The embryos in collagen pad were not flattening and kept polarity and spherical shape during culture. The polar trophoblast invaded the type I collagen downward unlike the horizontal growth in control. In the developmental stage of mouse blastocyst, there were significant differences between control and type I collagen group during day 4 and 5 culture. Conclusion: Blastocyst development was better in type I collagen group than control. Therefore, in vitro culture study using type I collagen could provide improved model for the establishment of blastocyst implantation study.

Near-Normalized Gene Expression Profiles in Bladder With Detrusor Overactivity in Rats With Bladder Outlet Obstruction After Deobstruction

Lee, Tack,Lim, U-Sung,Kang, Dong Hyuk,Jung, Hae-Do,Kim, Hyunzu,Choi, Bo-Hwa,Kang, Ju-Hee,Yoon, Sang-Min,Park, Chang-Shin Korean Continence Society 2017 International Neurourology Journal Vol.21 No.4

<P><B>Purpose</B></P><P>The pathophysiological role of detrusor overactivity (DO) in the bladder, which is commonly observed in various bladder diseases, is not well understood. DO appears in bladder outlet obstruction (BOO), and may continue even after subsequent deobstruction. DO therefore provides an excellent opportunity to observe molecular biological changes.</P><P><B>Methods</B></P><P>In this study, to understand the molecular effects of persistent DO after BOO induction and deobstruction, we performed awake cystometry on female Sprague-Dawley rats divided into 4 groups: a sham group, a BOO group, a deobstructed group with DO after BOO (DDO), and a deobstructed group without DO after BOO (non-DDO). Total RNA was extracted from the bladder samples, and gene expression profiles were compared between the sham and model groups.</P><P><B>Results</B></P><P>DO was observed in 5 of the 6 rats (83%) in the BOO group, and in 6 of the 13 rats (46%) in the deobstructed group. The non-DDO group showed a significantly greater residual volume than the DDO group. Through a clustering analysis of gene expression profiles, we identified 7,532 common upregulated and downregulated genes, the expression of which changed by more than 2 fold. In the BOO group, 898 upregulated and 2,911 downregulated genes were identified. The non-DDO group showed 3,472 upregulated and 4,025 downregulated genes, whereas in the DDO group, only 145 and 72 genes were upregulated and downregulated, respectively.</P><P><B>Conclusions</B></P><P>Abnormal function and gene expression profiles in bladders after BOO were normalized in the BOO rats with DO after deobstruction, whereas in those without DO, abnormal function persisted and the gene expression profile became more abnormal. DO may play a protective role against the stress to the bladder induced by BOO and deobstruction as a form of adaptive neuroplasticity.</P>

PET/CT 검사에서 환자체형에 따른 적정검사 프로토콜에 관한 고찰

조석원,함준철,강천구,반영각,이승재,임한상,이창호,박훈희,Cho, Seok Won,Ham, Joon-Chul,Kang, Chun Goo,Bahn, Young Kag,Lee, Seung Jae,Lim, Han Sang,Lee, Chang-Ho,Park, Hoon-Hee 대한핵의학기술학회 2013 핵의학 기술 Vol.17 No.2

Purpose: Whole-body PET using radiopharmaceutical is one of the imaging study methods for physiological changes of body. High specificity of the PET-CT examination is used to detect an early stages of cancer and metastatic cancer by imaging a physiological changes. During the imaging process, PET image has been characterized by a relatively low image quality due to its low sensitivity and the acquisition of random and scatter coincidences as well as patients figure. Therefore, the image quality as the changes of the acquisition times of patient weight was evaluated in this study. Materials and Methods: Thirty patients who presented to our hospital were enrolled. They were divided to normal, overweight, and obese group using BMI index, respectively. The patients with a liver disease and diabetes were excluded. $^{18}F-FDG$ was administered to the patients as 5.2 MBq per kg. After an hour from an injection, image acquisition was obtained as List mode in a part of liver in 1 bed. SNR (signal-to-noise ratio) of each groups acquisition times were confirmed from the calculated radiation counts and random fractions. The statistical significance of three groups was confirmed through one-way ANOVA test. On the basis of the counts of 2 minutes on normal group, the SNR of overweight group and obese group were compared. Results: The SNR were increased with loger aquisition time in 3 groups. In the condition of same acquisition time, the SNR had a statistical significance (P<0.05). The SNR were decreased to the normal, overweight, and obese, respectively. Liver activity had no significance difference on each group and RF had the significance differences (P<0.05). On the basis of the counts of 2 minutes on normal group, there were no statistical significance in a three minute acquisitions of overweight group and two minute acquisitions of obese group (P=0.150). Conclusion: In this study, the administrated amount of radiation dose did not adjust as the change of the patients weight. Increasing the acquisition time when the administration of the same amount of dose was able to get a good result of SNR. When the Based 2 minute on normal group, if overweight and obese case the increased acquisition time of 3 minute was able to obtain a similar SNR. On the basis of the normal group, the acquisition times of overweight and obese group were increased to 3 minutes per bed and the SNR were similar to the normal group.