A Study of the Two-Dimensional High-Performance Liquid Chromatogrphy for Total Amino Acid Sequencing of rbST

김정호,김훈주,김준,Kim, Jung-Ho,Kim, Hoon-Joo,Kim, Joon 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

트립신으로 절단한 재조합 소성장호르몬의 펠타이드 조각을 분리, 정제하기 위하여 강이온 교환 HPLC와 역상 HPLC의 2차원 고성능 액체 크로마토그래피법을 이용하였다. 1차원적으로 역상 HPLC만을 이용해 펩타이드를 분리하였을 때에는 두 가지 이상의 서로 다른 펩타이드가 섞여 있는 heterogeneous peak이 발견되였다. 그러나 2차원 HPLC에 의해 펩타이드를 분리 시켰을 때에는 고도로 정제된 펩타이드를 얻을 수 있였다. 트립신 절단 결과 생긴 펩타이드를 모두 분리시키기 위해 1차적으로 강 양이온 교환 HPLC를 시행하였다. 이 결과 9개의 서로 다른 peak를 분리해 냈으며, 각각의 peak들을 다시 역상 HPLC하여 2차적인 분리를 시도하였다. 여기에서 얻은 peak들을 아미노산 서열분석을 통해 명료한 서열 분석결과를 얻어 낼 수 있었다. 또한 이 과정을 통해 53번 Cys과 164번 Cys 그리고 181번 Cys 사이에 disulfide bond가 존재하고 있음도 동시에 확인할 수 있었다. In order to separate and purify tryptic digested recombinant bST (rbST), 2-dimensional HPLC which involves a combination of strong cation-exchange chromatography and reverse-phase chromatography was applied. A single dimension RP-HPLC resulted in several heterogeneous peaks. However, all fractions purified by two-dimensional chromatogrphy resulted in homogeneous peptides and unambiguous sequence data. With a view of separating perfectly, tryptic digest was first fractionated by strong cation-exchange chromatogrphy. The nine fractions were obtained from this chromatogrphy and each of these fractions was applied to RP-HPLC. Fractions yielded from RP-HPLC were submitted for direct sequence analysis and clear sequence data were obtained. In this study, the location of disulfide bonds, Cys53-Cys164 and Cys181-Cys189, also were confirmed in rbST.

진동/음향 해석에 의한 스피커의 음향특성 연구

김정호,김준태,김진오,민진기,Kim, Jung-Ho,Kim, Jun-Tai,Kim, Jin-Oh,Min, Jin-Ki 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.10

The acoustic characteristics of a direct radiator type loudspeaker has been studied in this paper. The natural modes of the speaker cone vibration analyzed numerically by the finite element method have been verified by comparing them with experimental results. The so-ap-proved finite-element model has been used to calculate the vibration response of the cone excited by the voice coil. The vibration displacement of the speaker cone paper has been converted into the vibration velocity and used as a boundary condition for the acoustic analysis. The frequency characteristics, directivity, and sound pressure distribution of the loudspeaker have been calculated by the boundary element method. The numerical results have been verified by the experiments carried out in an anechoic chamber. The variations of the acoustic characteristics due to the changes of some design parameter values can be examined using the numerical model.

강재로 구속된 프리스트레스트 콘크리트 합성거더의 정적 및 피로거동

김정호,박경훈,황윤국,이상윤,Kim, Jung Ho,Park, Kyung Hoon,Hwang, Yoon Koog,Lee, Sang Yoon 한국강구조학회 2004 韓國鋼構造學會 論文集 Vol.16 No.6

최근 국내에서 I형 거더의 외부가 강재로 이루어지고 내부에 무근콘크리트가 채워져 합성된 거더에 프리스트레스를 도입하여 경간을 장대화할 수 있는 새로운 개념의 합성거더인 '강재로 구속된 프리스트레스트 콘크리트 합성거더(Steel-Confined Prestressed Concrete Girder; SCP Girder)'가 개발되었다. 또한 개발된 거더를 실물크기로 제작하여 시공성을 확인하고 정적하중 재하에 의한 파괴실험을 통해 공용 중 안전성에 대한 실험적 검증이 수행되었다(김정호 등, 2002). 그러나 SCP 합성거더의 실용화를 위해서는 교량 파손의 주원인이 주행하중의 영향에 의한 피로파손인 점을 고려하여 반복하중에 대한 피로거동의 연구 및 피로 안전성 확보가 요구된다. 따라서 본 연구에서는 SCP 합성거더에 대한 피로거동 특성을 파악하고 피로설계를 위한 기초적인 자료를 제시하기 위해 피로실험을 실시하였다. 피로실험을 위한 시험체의 제작은 1등교에 해당하는 설계활하중(DB-24)에 대하여 10m 지간으로 설계 제작하였으며, 실험결과 도로교설계기준(한국도로교통협회, 2000)의 상세피로범주에 따른 허용피로응력범위를 초과하지 않는 것으로 나타났다. 또한 초기정적재하를 통해 설계의 타당성과 구조적 거동을 분석하였으며, 피로실험 종료 후 거더 외부를 이루고 있는 강재를 제거하여 내부콘크리트의 상태를 관찰하였다. A new type of girder, called steel-confined prestressed concrete girder (SCP girder), has been developed, which maximizes the structural advantages of concrete, steel, and PS tendon, and improves on the shortcomings of steel plate girder, PSC I-girder, and preflex girder bridge for use in the construction of middle- or long-span bridges. To verify the propriety of design, structural safety, and applicability of this girder, a static load test was carried out (Kim et al.., 2002). Since the main damage typically sustained by steel bridges results from the fatigue caused by the repetition of traffic loads, fatigue safety must therefore be guaranteed in applying the SCP girder in the construction of real bridges. In this study, a fatigue test was carried out to investigate fatigue behavior and provide basic data for fatigue design. Based on the fatigue test, the fatigue safety of the girder was estimated. For the fatigue test, 10-m specimens were designed for a standard-design truckload (DB-24). A static load test was also performed before the fatigue test to analyze the structural behavior of the specimens. After the fatigue test, outer steel plates were removed to observe the condition of the concrete in the girder.

공작실에서 실내 및 작업종사자의 중금속 오염도에 관한 고찰

김정호,김가중,김성기,배석환,Kim Jeong-Ho,Kim Gha-Jung,Kim Sung-Ki,Bea Suk-Hwan 대한방사선치료학회 2005 대한방사선치료학회지 Vol.17 No.2

Purpose : Heavy metal use when producing the block from the workshop. At this time, production of heavy metal dust and fume gives risk in human. This like heavy metal to improve seriousness through measurement and analysis. And by the quest in solution is purpose of this thesis. Materials and Methods : Organization is Inductively Coupled Plasma Atomic Emission Spectrometer, and the object is Deajeon city 4 workshops in university hospital radiation oncology (Bismuth, Lead, Tin and cadmium). Method is the ppb the pumping it does at unit, comparison analysis. And the Calculation heavy metal standard level in air through heavy metal standard level in body and blood, so Heavy metal temporary standard set. Results : Subterranean existence room air quality the administration laws appointed Lead and Cadmium's exposure recommend that it is $3{\mu}g/m^3\;and\;2{\mu}g/m^3$. And Bismuth and Tin decides $7{\mu}g/m^3\;and\;6{\mu}g/m^3$ through standard level in air heavy metal and standard level in body and blood. Heavy metal measurement level of workshops in 4 university hospital Daejeon city compares with work existence and nonexistence. On work nonexistence almost measurement level is below the recommend level. But work existence case express high level. Also consequently in composition ratio of the block is continuous with the detection ratio. Conclusion : Worker's heavy metal contamination imbrued serious for solution founds basic part. In hospital may operation on local air exhauster and periodical efficiency check, protector offer, et al. And worker have a correct understanding part of heavy metal contamination, and have continuous interest, health control. Finally, learned society sphere administer to establishment standard level and periodical measurement. And it founds basic solution plan of periodical special health checkup.

난관 세포와 공동 배양에 의한 배 세포 발달의 향상

김정호,홍기연,김기석,최정훈,민부기,Kim, Jung-Ho,Hong, Gi-Youn,Kim, Kie-Sock,Choi, Jung-Hoon,Min, Bu-Kie 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1

To improve in vitro embryonic cell development, this study was desigend to culture in vitro fertilized early embryos of mouse in two different systems; conditioned medium alone and ampullary cells co-culture. Thirty two of 83 embryos(38.6%) were blocked in the 2 cell stage by co-culture, as compared to forty of 42 embryos(95.2%) in control group for 24hours culture. And all the embryonic cells cultured for conditioned medium alone were blocked for 48 hours culture. Twenty seven of 46 embryos (58.7 %) which overcome culture block in 2 cell stage by cocultured were developed morular and expanded blastocyst, and ninteen of 46 embryos(26.1 %) underwent hatching for 96 hours culture. The cellular fragmented rates for embryo were 26.2% in medium alone; 10 fragmented blastomere were graded mild status and 1 fragmented blastomere in severe status. On the other hand, the fragmented rate for 48 hours co-cultured were 15.7%03/83); 8 fragmented embryos were graded mild status, moderate status in 3 fragmented embryos and severe in 2 fragmented embryos respectively. In conclusion, the co-culture of embryos with ampullary cells is good to improve quality of embryos and overcome of culture block as well as development of cell cleavage.

개착식 터널에서 파형강판 라이닝 적용을 위한 해석적 연구

김정호,정형식,Kim, Jung-Ho,Chung, Hyung-Sik 한국터널지하공간학회 2004 터널기술 Vol.6 No.1

기존의 터널 라이닝으로서 주를 이루고 있는 콘크리트 라이닝은 여러 문제점들이 발생하고 있으며 콘크리트 라이닝의 대제 재료에 대한 관심이 증대되고 있다. 본 논문에서는 파형강판을 개착식 터널 라이닝으로 도입하기 위한 초기 단계에 있어서 파형강판 라이닝의 유한요소 해석을 수행하고 CHBDC (Canadian Highway Bridge Design Code, 2000) 규정에 의해 안정성을 검토한 후 몇 가지 조건에 의해 발생되는 단면력의 경향을 분석함으로서 터널 라이닝으로서의 새로운 재료의 거동 특성을 규명하고자 하였다. 또한 현장에서의 시공 단계를 모사한 1/40 scale의 모형토조 실험과 동일한 scale의 수치해석을 수행하여 본 연구에서 사용한 수치해석 기법의 적정성을 검증하였다. A finite element method is used for the force analysis of semicircular arch shaped corrugated steel plate lining. The assessment of stability and behavior for several conditions are executed from the analysis of soil-structure interaction in accordance with CHBDC (Canadian Highway Bridge Design Code, 2000). One fortieth scaled model tests were conducted on the semicircular arch lining to verify the FEM analysis results under the earth-load conditions.

생산공정라인의 해석 및 운영표현기법

김정호,정태진,유종준,박중무,Kim, Jeong-Ho,Jeong, Tae-Jin,Yu, Jong-Jun,Park, Jung-Mu 한국전자통신연구원 1991 전자통신동향분석 Vol.6 No.2

최근의 생산자동화 분야에는 제품의 다양화, 라이프사이클의 단기화 등의 요구에 대응하기 위하여 유연성, 확장성, 보수성이 높은 공정 적용시스팀의 실현요구가 높아지고 있다. 이 요구에 대응하기 위하여 조립, 검사 등의 공정라인에서는 프로그램이 가능한 PLC, 로보트 등의 단위제어기기를 조합하여 작업스테이션을 구성하고 이들을 반송차 등으로 연결하여 운영하는 사례가 늘어나고 있다. 이러한 자동화의 실현을 가능하게 하기 위하여 대상공정의 단위 제어기기에 따라 라인 운용의 변경 등에 유연하게 대응하고 또한 재기동 조정, 이상처리 등의 지원기능을 보유하기 위한 공정구축의 해석과 제어시스팀의 운용기법이 연구되고 있다. 본고에서는 이러한 생산라인에서의 단위제어기기의 새로운 요구와 공정의 해석을 위하여 C-net와 Grafcet기법과 이의 활용사례를 소개한다.

대규모 시스팀의 해석 및 제어에 관한 연구동향

김정호,정태진,정하재,Kim, Jeong-Ho,Jung, Tae-Jin,Jung, Ha-Jae 한국전자통신연구원 1988 전자통신동향분석 Vol.3 No.1

대규모 시스팀의 해석 및 제어에 관한 연구동향을 시스팀의 축소화 기법, 안정도 검사, 비집중 제어, 계층적 제어로 기술하고, 이러한 대규모 시스팀의 연구분야로 자동생산 시스팀, 전력계통시스팀, 교통통제 시스팀, 사회공학 시스팀 분야로 분류하고 그 해석 및 제어에 관한 연구동향을 분석함으로써 이와 관련하여 추진되고 있는 연구분야를 소개하였다.

외상 후 발생한 심한 족관절 첨족 변형의 2단계 수술적 치료방법 - 예비보고 -

김정호,이준영,하상호,유재원,이상홍,나웅채,Kim, Jung-Ho,Lee, Jun-Young,Ha, Sang-Ho,You, Jae-Won,Lee, Sang-Hong,Na, Woong-Chae 대한족부족관절학회 2011 대한족부족관절학회지 Vol.15 No.2

Purpose: To evaluate the effectiveness as well as correct the post-traumatic severe ankle equinus deformity by conducting the treatment surgery, which is divided into 2 stages, soft tissue adhesiolysis and ankle arthrodesis. Materials and Methods: We have conducted the methods, which are Z-plasty Achilles tendon lengthening, multiple capsulotomy and tendon lengthening (flexor hallucis longus muscle, flexor digitorum longus, posterior tibialis tendon) for 10 patients who has shown equinus deformity after post-traumatic compartment syndrome due to the injury. The average age of patients was 33.7 year-old; there were 8 men and 2 women, and the follow up period was 13 months (6~31 mon). Outcomes were rated based on American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score, Visual Analogue Scale (VAS) and patient's satisfaction after operation. Results: The average degree of preoperative equinus deformity was 64 degree (-60~-70), and we've obtained AOFAS anklehindfoot score that was evaluated after 4 months of 2nd operation which was 76.7 score on average. We've also checked the decreased pain score from all of the patients according to the fact that the average VAS before the surgery was 6 (4~8), but it reduced to 3 (1~4). The patient's satisfaction showed generally great satisfactions which was 5 cases were excellent, 2 cases were good, and 3 cases were fair. Conclusion: The two staged surgical treatment of post -traumatic severe ankle equinus deformity, which was conducted of soft tissue adhesiolysis and arthrodesis, could be one of the effective methods to improve patients walking ability.

영과잉포아송회귀분석을 활용한 안정병동에 입원한 정신질환자의 공격행동 예측요인

김정호,신성희,Kim, Jung Ho,Shin, Sung Hee 경희대학교 동서간호학연구소 2022 동서간호학연구지 Vol.28 No.2

Purpose: This study was conducted to identify predictors related to aggressive behavior of patients with mental illness admitted to a closed psychiatric ward. Methods: This study adopted a retrospective design which analyzed the hospital medical records of 363 patients with mental illness admitted to the psychiatric closed ward of a university hospital in Seoul, Korea. The collected data were analyzed using SPSS IBM 20.0 and STATA 12.0 SE. ZIP (Zero-Inflated Poisson) and count data analysis were used for the factor influencing the occurrence and frequency of aggressive behavior. Results: The results of ZIP model showed that the factors influencing non-probability of aggressive behavior were anxiety, non-adherence, and frustration. In addition, the factors influencing frequency of aggressive behavior were bipolar disorder and personality disorder trait. Conclusion: We found that bipolar disorder, frustration, and non-adherence are more likely to increase the likelihood of aggressive behavior in patients with mental illness. In particular, patients diagnosed with bipolar disorder were 1.95 times more likely to engage in repetitive aggressive behavior compared to those without a diagnose. However, since the results were different form previous studies, further studies on the traits of anxiety and personality disorders are needed.