관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

연령대별 요추 추간판탈출증 환자의 요근과 척추주위근 단면적에 대한 분석

공덕현 ( Deok Hyun Kong ),배광호 ( Kwang Ho Bae ),고호연 ( Ho Yeon Go ),강명진 ( Myung Jin Kang ),김준수 ( Jun Su Kim ),양기영 ( Kee Young Yang ),이재훈 ( Jae Hoon Lee ),은영준 ( Young Jun Eun ) 한방재활의학과학회 2010 한방재활의학과학회지 Vol.20 No.4

Objectives :This study was designed to evaluate the correlation of the cross-sectional areas(CSA) of paraspinalis(iliocostalis lumborum, multifidus, longissimus thoracis parts lumborum) and psoas muscle and sex, age, change of the VAS(visual analogue scale) and past history of chronic low back pain in patients suffering from HIVD(herniation of intervertebral disc). Methods :Medical records of 140 subjects with HIVD were retrospectively reviewed. The MRI(magnetic resonance imaging) findings on the patients were scanned and analyzed by PiView program to find the paraspinal and psoas muscle CSA and were evaluated by the correlation of sex, age, change of the VAS, and past history of chronic low back pain. Results :There was significant difference between groups in male and female, young-adults age and middle age, middle age and old age, young-adults age and old age. But the correlation of the paraspinal and psoas muscle CSA and past history of chronic low back pain were partially significant. On the other hand, the correlation of the paraspinal and psoas muscle CSA and change of the VAS were not significant. Conclusions :These results suggest that the cross-sectional areas(CSA) of paraspinalis and psoas muscle have relevance to sex and age.

Direct effectiveness of pneumococcal polysaccharide vaccine against invasive pneumococcal disease and non-bacteremic pneumococcal pneumonia in elderly population in the era of pneumococcal conjugate vaccine: A case-control study

Kim, Jong Hun,Chun, Byung Chul,Song, Joon Young,Kim, Hyo Youl,Bae, In-Gyu,Kim, Dong-Min,Choi, Young Hwa,Jun, Yoon Hee,Choi, Won Suk,Kang, Seong Hee,Kwon, Hyun Hee,Jeong, Hye Won,Kee, Sae Yoon,Hur, Jia Elsevier Ltd. 2019 Vaccine Vol. No.

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>While herd effects and serotype replacement by childhood pneumococcal protein conjugated vaccines (PCVs) continues to accumulate worldwide, direct effectiveness of 23-valent pneumococcal polysaccharide vaccine (PPV23) against pneumococcal diseases in the elderly has been challenged. We estimated the direct effectiveness of PPV23 in the elderly population.</P> <P><B>Methods</B></P> <P>For a hospital-based case-control study, cases of invasive pneumococcal disease (IPD) and non-bacteremic pneumococcal pneumonia (NBPP) (adults ≥ 65 years) were identified in 14 hospitals participated in the pneumococcal surveillance program from March 2013 to October 2015, following implementation of PPV23 national immunization program (NIP) for the elderly in the Republic of Korea. Controls matched by age, sex, and hospital were selected at ratios of 1:2 (IPD) or 1:1 (NBPP). Clinical data and vaccination records were collected. Vaccine effectiveness was calculated as (1-adjusted odds ratio) × 100.</P> <P><B>Results</B></P> <P>We enrolled 148 IPD and 557 NBPP cases, and 295 IPD and 557 NBPP controls for analyses. Overall effectiveness of PPV23 against IPD was 28.5% [95% confidence interval (CI) −5.8%–51.6%] and against NBPP was 10.2% (-15.1-30.6) in all patients ≥ 65 years. However, in subgroup analysis of patients aged 65–74 years, PPV23 was protective against IPD [effectiveness 57.4% (19.4–77.5)] and against NBPP [effectiveness 35.0% (2.3–56.7)]. Furthermore, serotype-specific effectiveness of PPV23 against IPD was 90.6% (27.6–98.8) for PPV23-unique serotypes and 81.3% (38.6–94.3) for PPV23 serotypes excluding serotype 3.</P> <P><B>Conclusions</B></P> <P>This study indicates that PPV23 with broad serotype coverage might be beneficial in preventing IPD and NBPP due to non-PCV13 serotypes in the young-elderly, with potentially increasing effectiveness in the setting of childhood PCV NIP.</P> <P><B>Highlights</B></P> <P> <UL> <LI> National immunization program for PPV23 for the elderly was implemented in Korea. </LI> <LI> PPV23 was effective against IPD and NBPP for young elderly patients aged 65–74. </LI> <LI> Effectiveness of PPV23 against IPD was higher for PPV23 unique serotypes. </LI> <LI> PPV23 was non-protective against IPD caused by PCV13 serotypes. </LI> </UL> </P>

입원치료를 요하는 소아의 급성복증

배택환,김준식,유인술,조준필,이철주,민영기 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

The acute abdomen defined as an acute abdominal condition which needs immediate decision for treatment is one of the most common cause for emergency care. One thound six hundreds nine patients were visited to pediatric emergency center due to acute abdomen. To aid in early diagnosis and proper treatment, a clinical review was made on 278 cases of acute abdomen under 15 yrs old which admitted to Pediatrics and General surgery via emergency center from June of 1994 to May of 1995. The results as follows: It was 17.3% of total visiting to pediatric emergency center due to gastrointestinal sysmptoms. The sex ratio(Male: Female) was 2.35:1. Acute gastroenteristis was the most common acute abdominal condition(54%) and followed by acute appendicitis(17.9%), and intussusception(13.9%). The most common chief commplaint was vomiting(58%) and followed by abdominal pain(39.4%), diarrhea(35.8%), and fever(42.1%). One hundred fifty-five patients were visited to pediatric emergency center via local clinic and thirty patients via OPDs. The operations were done in 75 patients. Among them, immediate operation were done in 60 patients and delayed operations were done in 15 patients, but there is no difference in complication.

방선균 J-144K가 생산하는 Adenosine Deaminase Inhibitor에 관한 연구

전홍기,김상웅,조영배,이인 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

Adenosine deaminase의 새로운 저해제를 검색하기 위해 토양으로부터 adenosine deaminase 저해제를 생산하는 8균주의 방선균을 분리하였으며, 그중에서 저해활성이 가장 우수한 J-144K균주를 공시균주로 선정하였다. Adenosine deaminase 저해제 생산을 위한 최적배지 조성은 1.0% dextrose, 0.5% yeast extract, 0.5% peptone, 0.1% KH_2PO_4이었으며, 초적온도와 pH는 각각 30˚C와 7.0이었다. 이러한 조건에서 500ml용 진탕플라스크에 최적배지 100ml를 넣어 배양했을 경우 정지기인 배양 60시간째에 adenosine deaminade 저해활성이 가장 높게 나타났다. 분리균주 J-144K의 배양상등액으로부터 활성탄 흡착 및 유기용매 추출, Dowex 50 H^+ X-8 column chromatography, Dowex 1 Cl^- X-8 column chromatography, Sephadex G-15 gel filtration 등의 과정을 거쳐 adenosine deaminase 저해제를 정제한 결과, 3가지 종류의 저해제가 존재함을 알 수 있었다. In the screening of actinomycetes' culture filtrate for inhibitor of adenosine deaminase, a novel inhibitor was found in a cultured broth of strain J-144K. The optimum conditions for the adenosine deaminase inhibitor production from the isolated strain J-144K were evaluated. This strain showed the maximum yield of adenosine deaminase inhibitor when grown at pH 7.0 and 30˚C for 60 hours in the medium of 1.0% dextrose, 0.5% yeast extract, 0.5% peptone and 0.1 % KH_2PO_4 under the aerobic condition. Through the activated charcoal extraction, methanol fractionation, Dowex 50 H^+X-8 ion exchange column chromatography, Dowex Cl^- X-8 ion exchange column chromatography, and Sephadex G-15 gel filtration procedures, this inhibitor was purified with three materials.

SO₂감지를 위한 NASICON 고체전해질 가스센서의 제조 및 특성 평가

배재철,Liu, aiguo,이상태,전희권,방영일,이덕동,허증수 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

Human Rotavirus 위장관염에 대한 임상적 고찰

배현,이홍준,서우철,양은석,문경래,박영봉 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

We observed clinical features of 161 patients of acute gastroenteritis who were adimitted to the Department of Pediatrics in the Chosun University Hospital from January 1. 1989 to December 31. 1993. The stool specimen on admission was tested for rotavirus Ag by ELISA(Rotazyme Ⅱ^R)method. The results are as follows : 1) HRV antigen was detected in 97 patients (58.4%) among 161 patients with gastroenteritis. 2) In monthly distribution. 77.7% of cases developed between October and December 3) The majority if patients were under 24month of age with the peak incidnce being between 6-12 month of age. 4) Mean frequency of diarrhea per day were 5.3±2.6 days in HRV antigen rhinorrhea(48.5%), cough(44.7%). Dehydration was noted 87.2% of cases and 93.9% Was isotonic dehydration. 6) The mean duration of hospitalization was 5.3±2.6 days. 7) AST and AIT elevation in HRV group were 91.5%, 42.6% respectively. AST and ALT elevation in Non-HRV group were 73.1%, 26.9% respectively. AST elevation between two group was not correlated statistically. but ALT concentration was significantly increased in HRV group above normal than Non-HRV group (p<0.05%).

재식한 흰쥐 구치 치수조직의 재혈관화와 CGRP 면역반응 신경섬유의 변화

배용철,이승봉,김영진,남순현,김현정 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.4

치아 재식 후 시간경과에 따른 치수조직의 재혈관화와 신경재지배의 변화를 관찰하기위해 생후 35일된 흰쥐(Sprague dawley) 56마리를 대상으로 상악우측 제1대구치를 발치 즉시 재식한 후 1일, 2일, 3일, 1주, 2주, 4주, 6주 간격으로 희생시켜 먹물을 이용한 혈관조영술과 CGRP를 이용한 면역조직화학적방법으로 각기 시행하여 다음과 같은 결론을 얻었다. 1. 재혈관화와 CGRP 면역반응신경섬유의 재지배는 재식후 1일에서 근심측 치수조직에서는 이미 이루어졌으나 원심측은 미약했다. 재식후 2일에 전치수조직에서 재혈관화와 신경재지배가 이루어졌으며 이후 6주까지 시간경과에 따라 분포밀도가 증가하였으나 대조군에 비해서는 약하게 나타났다. 2. 재식후 1주에서부터 술후 상아질이 형성되기 시작하였으며 재식후 6주에서는 대조군에 비해 상대적으로 치수강이 좁아진 양상을 보였다. 3. 재혈관화와 신경재지배는 거의 같은 시기에 이루어지는 것으로 나타났으며 밀접한 관련성이 있는 것으로 보인다. The purpose of this study was to investigate the revascularization and reinnervation of calcitonin gene-related peptide immunoreactive nerves in immediately replanted rat molars. First maxillary right molars in 56 rats(35days old) were extracted and immediately replanted. The rats were killed 1, 2, 3, 7, 14, 28 and 42 days after replantation and revascularization of pulpal blood vessels were examined microangiogram with korean traditional ink and reinnervation of pulpal nerve were examined immunohistochemical method using calcitonin gene-related peptide(CGRP) antiserum. The results were as follows; 1. Revascularization and reinnervation of CGRP immunoreactive nerve fibers were observed mesial side whole pulp tissue of replanted teeth. Revascularization and reinnervation of CGRP immunoreactive fiber were made at 2days after replantation in entire pulp of replanted teeth and the distribution density of blood vessels were gradually increased according time elapsed, but did not achieve the density of control. 2. Postoperative dentin formation in replanted teeth revealed at 1 week after replantaton and gradually increased according to time elapsed. 3. Revascularization and Reinnervation of CGRP immunoreactive nerve fibers were established at the same time and it seems to be closed relatationship between revascularization and reinnervation.

약물전달체로서의 고분자 미셀의 특징 및 고분자 미셀을 이용한 제품

김준배, 손진영, 이은주, 조정원 충남대학교 약학대학 의약품개발연구소 2017 藥學論文集 Vol.32 No.-

The amphiphilic block copolymers forms the self -assembled micelles in aqueous solution. that is called as polymeric micelle. Polymeric micelles as drug delivery carriers can protect the drug against bio-logical environments (eg. low pH. enzymes). allow drug ellcapsulation in the hydrophobic inner center. in-crease the solubility of hydrophobic drugs). facilitate drug targeting. and reduce the side effects of chemotherapy. Another advantage is that polymeric micelles can prolong the circulation time of the drug us-ing the ligand for the specific target because the surface properties of the drug delivery system affect the circulation time in the blood and are related to the life span of the drug delivery system. Recent advances in the properties and application of polymeric micelles as drug delivery carriers for poorly soluble drug have been discussed in this review.