The Effect of Functional MA Film Packaging on Storage of Mature-Red Tomato

Guan, Jun-Feng,Park, Hyung-Woo,Kim, Yoon-Ho,Kim, Sang-Hee,Park, Hye-Ran,Lee, Seon-Ah,Yoon, Ji-Yoon Korea Society of Packaging Science and Technology 2005 한국포장학회지 Vol.11 No.2

토마토(Pinky 종)를 기능성 MA필름(두께 0.03, 0.06mm)으로 포장하여 0, 5, $15^{\circ}C$에 저장하면서 품질 변화를 조사하였다. 저장 29일에 MA포장재 내의 $CO_2$, $O_2$농도는 각각 $4.21{\sim}8.17%$, $9.78{\sim}14.45%$였고, 전반적으로 MA 포장구에서 연화와 색택의 변화가 억제되었다. 저장 29일에 MA포장(0.03두께)을 한 후 $5^{\circ}C$에 저장한 토마토의 경도(0.49 kgf)가 가장 높았고, MA 포장(0.06두께)을 한 후 $0^{\circ}C$에 저장한 토마토가 최소 라이코펜 함량(6.08mg/100g)을 보였다. 토마토의 품질 유지를 위한 최적 조건은 MA 장을 하여 $0^{\circ}C$에 저장하는 것이 바람직한 것으로 나타났으며 필름 두께간에는 유의적인 차이가 나타나지 않았다. 또한 MA 포장재 내의 최적 가스 조성은 $CO_2$는 $4.2%{\sim}5.6%$, $O_2$는 $14.3%{\sim}14.5%$로 나타났다. The mature-red tomato fruits (cv. Pinky world) were packaged with functional modified atmosphere (MA) film (0.03, 0.06 mm) and stored at 0, 5, $15^{\circ}C$. The results showed that the concentrations of $CO_2$ and $O_2$ inside MA package were about $4.21{\sim}8.17%$ and $9.78{\sim}14.45%$ under steady state during storage 29 days at 0, $5^{\circ}C$, respectively. The MA packaging(MAP) suppressed the fruits softening and the color change. The minimum lycopene content and maximum firmness were separately observed in fruits packaged with MA film (0.03 mm) at $5^{\circ}C$ storage and film(0.06 mm) at $0^{\circ}C$, which the lycopene content 6.08 mg/100 g, and firmness was 0.49 Kgf at 29 th day storage. The optimal conditions to keep high quality of tomato fruits were obtained by MAP during storage at $0^{\circ}C$ for 29 days, independently of the film thickness, in which the adequate gas composition was $CO_2$ at $4.2%{\sim}5.6%$, $O_2$ at $14.3%{\sim}14.5%$, respectively.

칼슘처리와 MA포장이 딸기의 품질에 미치는 영향

( Jun Feng Guan ),박형우 ( Hyung Woo Park ),김윤호 ( Yoon Ho Kim ),김상희 ( Sang Hee Kim ),박혜란 ( Hye Ran Park ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2006 한국식품저장유통학회지 Vol.13 No.1

The Effect of Functional MA Film Packaging on Storage of Mature-Red Tomato

Jun Feng Guan,Hyung Woo Park,Yoon Ho Kim,Sang Hee Kim,Hye Ran Park,Seon Ah Lee,Ji Yoon Yoon 한국포장학회 2005 한국포장학회지 Vol.11 No.2

토마토(Pinky 종)를 기능성 MA필름(두께 0.03, 0.06mm)으로 포장하여 0, 5, 15℃에 저장하면서 품질 변화를 조사하였다. 저장 29일에 MA포장재 내의 CO2, O2농도는 각각 4.21~8.17%, 9.78~14.45%였고, 전반적으로 MA 포장구에서 연화와 색택의 변화가 억제되었다. 저장 29일에 MA포장(0.03두께)을 한 후 5℃에 저장한 토마토의 경도(0.49 kgf)가 가장 높았고, MA 포장(0.06두께)을 한 후 0℃에 저장한 토마토가 최소 라이코펜 함량(6.08mg/100g)을 보였다. 토마토의 품질 유지를 위한 최적 조건은 MA 장을 하여 0℃에 저장하는 것이 바람직한 것으로 나타났으며 필름 두께간에는 유의적인 차이가 나타나지 않았다. 또한 MA 포장재 내의 최적 가스 조성은 CO2는 4.2%~5.6%, O2는 14.3%~14.5%로 나타났다. The mature-red tomato fruits (cv. Pinky world) were packaged with functional modified atmosphere (MA) film (0.03, 0.06 mm) and stored at 0, 5, 15℃. The results showed that the concentrations of CO2 and O2 inside MA package were about 4.21~8.17% and 9.78~14.45% under steady state during storage 29 days at 0, 5℃, respectively. The MA packaging(MAP) suppressed the fruits softening and the color change. The minimum lycopene content and maximum firmness were separately observed in fruits packaged with MA film (0.03 mm) at 5℃ storage and film(0.06 mm) at 0℃, which the lycopene content 6.08 mg/100 g, and firmness was 0.49 Kgf at 29 th day storage. The optimal conditions to keep high quality of tomato fruits were obtained by MAP during storage at 0℃ for 29 days, independently of the film thickness, in which the adequate gas composition was CO2 at 4.2%~5.6%, O2 at 14.3%~14.5%, respectively.

Reports ; Adrenergic receptor β2 activation by stress promotes breast cancer progression through macrophages M2 polarization in tumor microenvironment

( Jun-fang Qin ),( Feng-jiao Jin ),( Ning Li ),( Hai-tao Guan ),( Lan Lan ),( Hong Ni ),( Yue Wang ) 생화학분자생물학회 2015 BMB Reports Vol.48 No.5

Stress and its related hormones epinephrine (E) and norepinephrine (NE) play a crucial role in tumor progression. Macrophages in the tumor microenvironment (TME) polarized to M2 is also a vital pathway for tumor deterioration. Here, we explore the underlying role of macrophages in the effect of stress and E promoting breast cancer growth. It was found that the weight and volume of tumor in tumor bearing mice were increased, and dramatically accompanied with the rising E level after chronic stress using social isolation. What is most noteworthy, the number of M2 macrophages inside tumor was up-regulated with it. The effects of E treatment appear to be directly related to the change of M2 phenotype is reproduced in vitro. Moreover, E receptor ADRβ2 involved in E promoting M2 polarization was comprehended simultaneously. Our results imply psychological stress is influential on specific immune system, more essential for the comprehensive treatment against tumors. [BMB Reports 2015; 48(5): 295-300]

Screening Peptides Binding Specifically to Colorectal Cancer Cells from a Phage Random Peptide Library

Wang, Jun-Jiang,Liu, Ying,Zheng, Yang,Liao, Kang-Xiong,Lin, Feng,Wu, Cheng-Tang,Cai, Guan-Fu,Yao, Xue-Qing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancer cells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normal colorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phage display peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay and immunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds of screening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPM motif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide library and may be used as potential vectors for targeting therapy for colorectal cancer.

Comparative Proteomics Analysis of Colorectal Cancer

Wang, Jun-Jiang,Liu, Ying,Zheng, Yang,Lin, Feng,Cai, Guan-Fu,Yao, Xue-Qing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background and Objective: Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins. Materials and Methods: Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, with identification on the basis of MALDI-TOF mass spectra. Results: Compared with normal colorectal mucosa, protein abnormal expression of 65 spots varying more than 1.5 times were found in 2-DE gels from colorectal cancer samples (P<0.05); forty-two proteins were up-regulated and 23 were down-regulated; twelve protein spots were identified using mass spectrometry, of which 8 were up-regulated, includimng HSPB1and Annexin A4, while 4 were down-regulated, the results being consistent with Western blot findings. Conclusions: Two-dimensional electrophoresis reference maps for CRC tissues and adjacent normal mucosa (NMC) were established and 12 differentially expressed proteins were identified. Up-regulated HSPB1 and Annexin A4 may play many important roles in the pathogenesis of colorectal cancer.

Association of Rs11615 (C>T) in the Excision Repair Cross-complementing Group 1 Gene with Ovarian but not Gynecological Cancer Susceptibility: a Meta-analysis

Ma, Yong-Jun,Feng, Sheng-Chun,Hu, Shao-Long,Zhuang, Shun-Hong,Fu, Guan-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Evidence suggests that the rs11615 (C>T) polymorphism in the ERCC1 gene may be a risk factor for gynecological tumors. However, results have not been consistent. Therefore we performed this meta-analysis. Methods: Eligible studies were identified by search of PubMed, MEDLINE and Chinese National Knowledge Infrastructure (CNKI). Odds ratios (ORs) and 95% confidence intervals (CIs) were applied to assess associations between rs11615 (C>T) and gynecological tumor risk. Heterogeneity among studies was tested and sensitivity analysis was applied. Results: A total of 6 studies were identified, with 1,766 cases and 2,073 controls. No significant association was found overall between rs11615 (C>T) polymorphism and gynecological tumors susceptibility in any genetic model. In further analysis stratified by cancer type, significantly elevated ovarian cancer risk was observed in the homozygote and recessive model comparison (TT vs. CC: OR=1.69, 95% CI=1.03-2.77, heterogeneity=0.876; TT vs. CT/CC: OR=1.72, 95% CI=1.07-2.77, heterogeneity=0.995). Conclusion: The results of the present meta-analysis suggest that there is no significant association between the rs11615 (C>T) polymorphism and gynecological tumor risk, but it had a increased risk in ovarian cancer.

Centrifuge Modelling and Analysis of Ground Reaction of High-speed Railway Embankments over Medium Compressibility Ground

Chong-lei Zhang,Guan-lu Jiang,Xian-feng Liu,Li-jun Su 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.12

The objective of this study is to verify the feasibility of using centrifugal model tests to characterize the ground reaction response of a high-speed railway embankment to predict its ground settlement. To do so, a series of Centrifugal Model Tests (CMTs) of the embankment were performed to investigate the variation of ground reaction and corresponding derived modulus at different embankment ratios. The ground reaction determined from the CMTs was compared with those obtained from corresponding fullscale field tests of instrumented embankment. The results show that the ground reaction at the embankment centreline gradually approaches the embankment weight (γH) with increasing embankment ratio. Considering the embankment filling process with different embankment ratios, it seems inappropriate to employ the value of γH as the ground reaction without any correction in settlement prediction. Finally, the predictive capability of the proposed approach for embankment settlement estimation was assessed against the corresponding field measurements and those determined based on the calculation methods suggested in the current design standard in China. The results show that the proposed simple approach satisfactorily estimates the ground settlement of HSR embankments and its deviation of predicted settlements is below 11.9% compared with the data obtained from the field full-scale tests. However, the deviation of the result calculated by the current recommend design standard is 17.7%-148.3%.

Physicochemical and Sensory Characterization of Ginger-juice Yogurt during Fermentation

Guo-Hao Yang,Jun-Jun Guan,Jin-Shui Wang,Haicheng Yin,Fa-Dong Qiao,Feng Jia 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6

The primary purpose of this study was to investigate the effects of a ginger (Zingiber ofcinale) juice addition to milk on the physicochemical and sensory properties and consumer acceptability of yogurt during fermentation. Under the presence of ginger juice at 2.0 to 10.0%(v/v), there was a significant influence (p<0.05) on the viability of characteristic microorganisms in yogurt,the pH, titratable acidity (ºSH), viscosity, and hardness of the products. The descriptive data showed significant differences (p<0.05) among the samples for all attributes. The consumer results from external preference mapping indicated that samples with the lower ginger juice (≤4%)were liked over the higher samples (≥6%), with a negative impact on the acceptance by the consumers. Therefore,these findings suggest that 4%(v/v) may be the more appropriate content of ginger juice in the preparation of ginger-juice yogurt.

Neoadjuvant Chemotherapy-Guided Bladder-Sparing Treatment for Muscle-Invasive Bladder Cancer: Results of a Pilot Phase II Study

Hongzhe Shi,Wen Zhang,Xingang Bi,Dong Wang,Zejun Xiao,Youyan Guan,Kaopeng Guan,Jun Tian,Hongsong Bai,Linjun Hu,Chuanzhen Cao,Weixing Jiang,Zhilong Hu,Jin Zhang,Yan Chen,Shan Zheng,Xiaoli Feng,Changlin 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Reduced quality of life after cystectomy has made bladder preservation a popular research topic for muscle-invasive bladder cancer (MIBC). Previous research has indicated significant tumor downstaging after neoadjuvant chemotherapy (NAC). However, maximal transurethral resection of bladder tumor (TURBT) was performed before NAC to define the pathology, impacting the real evaluation of NAC. This research aimed to assess real NAC efficacy without interference from TURBT and apply combined modality therapies guided by NAC efficacy. Materials and Methods Patients with cT2-4aN0M0 MIBC were confirmed by cystoscopic biopsy and imaging. NAC efficacy was assessed by imaging, urine cytology, and cystoscopy with multidisciplinary team discussion. Definite responders (≤ T1) underwent TURBT plus concurrent chemoradiotherapy. Incomplete responders underwent radical cystectomy or partial cystectomy if feasible. The primary endpoint was the bladder preservation rate. Results Fifty-nine patients were enrolled, and the median age was 63 years. Patients with cT3-4 accounted for 75%. The median number of NAC cycles was three. Definite responders were 52.5%. The complete response (CR) was 10.2%, and 59.3% of patients received bladder-sparing treatments. With a median follow-up of 44.6 months, the 3-year overall survival (OS) was 72.8%. Three-year OS and relapse-free survival were 88.4% and 60.0% in the bladder-sparing group but only 74.3% and 37.5% in the cystectomy group. The evaluations of preserved bladder function were satisfactory. Conclusion After stratifying MIBC patients by NAC efficacy, definite responders achieved a satisfactory bladder-sparing rate, prognosis, and bladder function. The CR rate reflected the real NAC efficacy for MIBC. This therapy is worth verifying through multicenter research.