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김주현 ( Juhyun Kim ),남기원 ( Kiwon Nam ),김건영 ( Gunyoung Kim ),배준우 ( Junwoo Bae ),김성석 ( Sungsuk Kim ),장영은 ( Youngeun Chang ) 한국정보처리학회 2018 한국정보처리학회 학술대회논문집 Vol.25 No.1
최근 우리 사회에도 다문화가정이 증가하고 있다. 만약 부모가 한국어에 익숙하지 않다면, 그 자녀들도 유아기 한글 학습에 지장을 받을 수 있다. 이에 따라 다문화가정 유아의 특성을 반영한 한글교육 프로그램이 필요하다. 본 연구는 그 초기 단계의 연구 결과물을 담고있다. 즉, 학습 콘텐츠 개발자-서버 관리자-학습자를 위한 시스템을 설계하였고, 지금까지 개발된 한글쓰기 교육용 앱의 초기 버전을 개발 완료하였다.
Kim, Juhyun,Yeom, Jinki,Jeon, Che Ok,Park, Woojun Microbiology Society 2009 Microbiology Vol.155 No.7
<P>The growth pattern of Pseudomonas putida KT2440 in the presence of glucose and phenylacetic acid (PAA), where the sugar is used in preference to the aromatic compound, suggests that there is carbon catabolite repression (CCR) of PAA metabolism by glucose or gluconate. Furthermore, CCR is regulated at the transcriptional level. However, this CCR phenomenon does not occur in PAA-amended minimal medium containing fructose, pyruvate or succinate. We previously identified 2-keto-3-deoxy-6-phosphogluconate (KDPG) as an inducer of glucose metabolism, and this has led to this investigation into the role of KDPG as a signal compound for CCR. Two mutant strains, the edd mutant (non-KDPG producer) and the eda mutant (KDPG overproducer), grew in the presence of PAA but not in the presence of glucose. The edd mutant utilized PAA even in the presence of glucose, indicating that CCR had been abolished. This observation has additional support from the finding that there is high phenylacetyl-CoA ligase activity in the edd mutant, even in the presence of glucose+PAA, but not in wild-type cells under the same conditions. Unlike the edd mutant, the eda mutant did not grow in the presence of glucose+PAA. Interestingly, there was no uptake and/or metabolism of PAA in the eda mutant cells under the same conditions. Targeted disruption of PaaX, a repressor of the PAA operon, had no effect on CCR of PAA metabolism in the presence of glucose, suggesting that there is another transcriptional repression system associated with the KDPG signal. This is the first study to demonstrate that KDPG is the true CCR signal of PAA metabolism in P. putida KT2440.</P>