Cyclophosphamide가 흰쥐 혀침샘의 점액질에 미치는 영향에 대한 조직화학

조운복,정길남,조기진,이응희 釜山大學校 師範大學 科學敎育硏究所 2006 科學敎育硏究報 Vol.33 No.-

항암 및 항종양 치료제인 CP가 흰쥐의 혀 미각샘과 점액샘의 형태 및 그 분비물인 점액질에 미치는 영향을 PAS, AB pH 2.5, AB pH 2.5-PAS, AB pH 1.0, AF pH 1.7-AB pH 2.5 및 HID-AB pH 2.5 염색법으로 연구하였다. 1. CP군의 혀 미각샘의 형태적인 변화는 장액샘꽈리의 위축 및 파괴, 장액세포의 세포질과립의 감소 및 소실, 세포질의 공포변성 그리고 일부 도관상피세포의 점액화였으며, 혀 점액샘의 형태적 변화는 점액샘꽈리의 확장, 융합, 파괴, 점액세포의 세포질과립 감소 및 소실, 세포질의 공포변성 그리고 도관상칙의 현저한 점액화였다. 2. 혀 미각샘의 중성점액질이 대조군에 비해 현저히 감소하였으며 극소수의 미각샘세포에서 산성 점액질이 나타났다. 혀 점액샘의 중성점액질과 산성점액질은 대조군에 비해 다같이 현저히 감소하였고 산성점액질 중 sulfomucin은 현저히 감소하였으나 sialomucin은 다소 증가하는 경향을 나타내었다. The effect of cyclophophamide(CP) on the mucins of rat lingual salivary gland was examined by prelectin histochemical methods. Sprague-Dawley rats weighing about 250-300 gm were divided into control and CP groups. Control group was administrated by only drinking water for one week, CP group which was admirdstrated by 0.04% CP in the drinking water for one week. Mucins properties were stained with PAS, AB pH 2.5, AB pH 2.5-PAS, AB pH 1.0, AF pH 1.7-AB pH 2.5 and HID-AB pH 2.5 methods. 1. For the morphologic changes, the lingual gustatory glands of CP group, compared with the control group, were noted atrophy of serous adrd, decrease and disapperance of zymogen granules and vacuolation tn the serous cell. The mucification of duct cells was appeared in CP group. And the lingual mucous glands of CP group, compared with the control group, showed atrophy of mucous acini, and decrease of mudgen granules in mucous cells. Especially, many enlarged, fused mucous acini, and vacuolated mucous cells were detected. The mucification of duct cells increased very notably in CP group. 2. Neutral mucin of the lingual gustatory gland, and add and neutral mucins of lingual mucous gland decreased very remarkably in CP group compared with the control group. Especially, the decrease of acid mucin was more remarkable than that of neutral mucin in the mucous gland. But, in CP group, the mucous cells which secrete sulfomudn decreased, and the mucous cells which secrete sialomucin increased remarkably.

흰쥐 혀 침샘 점액질에 미치는 paraquat 독성에 대한 녹차의 완화효과

조운복,정길남,조기진 釜山大學校 師範大學 2003 교사교육연구 Vol.42 No.-

흰쥐의 혀 미각샘 및 점액샘과 그 점액질에 미치는 paraquat 독성과 녹차의 paraquat 독성완화 효과를 대조군, paraquat 투여군, Paraquat-녹차 투여군, 녹차 3주 투여후 paraquat-녹차 투여군으로 나누어 prelectin 조직화학법으로 연구하였다. Paraquat 투여군 미각샘은 대조군에 비해 장액샘꽈리 위축, 장액세포의 과립의 감소 및 소실, 공포변성 등이, 점액샘은 점액샘꽈리의 위축, 확장 또는 융합, 점액세포의 과립의 감소, 공포변성 및 미성숙 샘꽈리 등이 대조군에 비해 많이 관찰되었다. 미각샘 및 점액샘 도관상피의 점액화도 관찰되었다. Paraquat-녹차 투여군과 녹차 3주 투여후 paraquat-녹차 투여군에서는 paraquat 투여군의 미각샘과 점액샘의 형태적 변화가 대조군으로 회복되는 경향을 나타내었으며 후자에서 더 현저하였다. Paraquat 투여군의 미각샘의 중성점액질과 점액샘의 산성 및 중성점액질 양이 대조군에 비해 현저히 감소하였다 Paraquat 투여군의 혀 점액샘에서 sulfomucin을 분비하는 점액세포는 대조군에 비해 현저히 감소하였으나 sialomucin을 분비하는 점액세포는 오히려 증가하는 경향을 나타내었다. Paraquat-녹차 투여군과 녹차 3주 투여후 paraquat-녹차 투여군에서 대조군으로 회복되는 경향을 보였으며 후자에서 더 현저하였다. 이상의 결과로 보아 paraquat는 혀 미각샘 및 점액샘과 그 분비물인 점액질에 강한 독성을 나타내며 이 독성에 대해 녹차가 완화효과를 나타냄을 알 수 있고 녹차 3주 투여후 paraquat-녹차 투여군에서 녹차-paraquat 투여군보다 완화효과가 더 크게 나타나는 것으로 보아 paraquat 독성에 대한 완화효과는 녹차를 투여하는 시기와 그 양에 따라 더 효과가 있고 녹차에 paraquat 독성 발현억제 및 예방물질이 있음이 사료되어진다. Paraquat toxicity and the alleviating effects of green tea on the toxicity to the rat lingual mucins were investigated through prelectin histochemical methods. Mucosubstances properties were stained with PAS, AB pH 2.5, AB pH 1.0, AB pH 2.5-PAS, AF pH 1.7-AB pH 2.5 and HID-AB pH 2.5 methods. Sprague-Dawley male adult rats(250∼300g) were divided into one control group and three experimental groups, DP group which was administrated by only 0.04% paraquat in the drinking water for one week, GP group which was fed by both 0.04% paraquat and 3% green tea for one week, and GGP group which was applied with both 0.04% paraquat and 3% green tea for one week after 3% green tea in the drinking water was dosed for three weeks. For the morphological changes, the lingual gustatory glands of DP group, compared with the control group, were noted atrophy of serous acini, decrease and disappearance of zymogen granules, and vacuolation in the serous cells. And the lingual mucous glands of DP group showed atrophy of mucous acini, and decrease of mucigen granules. Especially, many enlarged, fused mucous acini, and vacuolated mucous cells were detected. Mucification of duct cells increased very notably in DP group. The groups of feeding green tea showed recovering tendency. The recovery degree of GGP group was greater than that of GP group. And GGP group recovered almost up to the state of the control group. Neutral mucin of the gustatory gland, and acid and neutral mucins of mucous gland decreased very notably in DP group compared with the control group. The decrease of acid mucin was more notable than that of neutral mucin in the mucous gland. And DP group showed that the mucous acini which secrete strong sulfomucin decreased, but the mucous acini which secrete sialomucin increased remarkably. GP and GGP group appeared the recovering tendency to the control group. The recovery of GGP group, comparing the degree, was more remarkable than that of GP group. And GGP group recovered almost up to the state of the control group. Consequently, the above results suggest that paraquat exhibites the toxicity which affects the histological structure and mucosubstances properties in the lingual salivary glands, and also suggest that green tea alleviates this toxicity. The different results of recovering tendency of paraquat toxicity in GP and GGP group indicate that the alleviating effects of green tea on paraquat toxicity to the histological structure and mucosubstances in the lingual salivary glands depend on the dosing time and the amount of green tea. Furthermore, green tea is presumed to have effects to recover from paraquat toxicity, to suppress exhibition of paraquat toxicity and to prevent paraquat toxicity.

피조개 발의 점액질에 대한 조직화학적 연구

최병태,조기진,홍말숙,정권순,조운복 釜山大學校 師範大學 1995 교사교육연구 Vol.31 No.-

피조개 발의 조직학적 구조는 hematoxylin-eosin염색 및 Weigert iron hematoxylin염색을, 점액질 성상을 검색하기 위하여 PAS반응, diastase 침지 후 PAS반응, alcian blue pH 2.5염색, alcian blue pH 1.0염색, alcian blue pH 2.5-PAS염색, alcian blue pH 1.0-PAS염색, aldehyde fuchsin pH 1.7-alcian blue pH 2.5염색 및 0.25% toluidine blue염색을 실시하였다. 피조개의 발은 상피, 상피하결합조직 및 근층으로 이루어져 있었으며 상피는 단층섬모원주세포로 되어 있었다. 상피하결합조직 및 근층에 다세포성 점액선인 발선이 발달되어 있었고 도관은 결합조직과 상피세포를 거쳐 개구되어 있었다. 근층은 종주근, 수직근 및 횡근섬유로 구성되어 있었다. 점액질의 조성을 보면 상피세포섬모에는 sialomucin만을, 상피세포는 중성점액질만 함유하고 있었다. 상피세포 및 결합조직내 점액도관에는 산성과 중성점액질의 혼합성인 것과 산성점액질만 함유한 것이 혼재하나 전자가 더 많았다. 산성점액질의 성상으로 보아 대부분이 강 sulfomucin을 가지며 일부는 약 sulfomucin과 sialomucin의 혼합성이었다. 대부분 발선의 점액선포에 대한 점액질 조성은 점액도관과 유사하였으나 일부 상부발선의 점액선포는 중성점액질만을, 하부발선의 점액선포는 sialomucin만을 함유하였다. This study was performed to investigate the histological structure and the histochemical properties of the mucosubstances in the foot of Anadara broughonii. For the histological structure, hematoxylin-eosin and Weigert iron hematoxylin staining were used. The mucosubstances stained with PAS, PAS-diastase, 0.25% toluidine blue, alcian blue pH 2.5, alcian blue pH 1.0, alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS, aldehyde fuchsin pH 1.7-alcian blue pH 2.5 methods. The foot was composed basically of three layers: epithelium, subepithelial connective tissue and muscular layers. The foot was lined by simple ciliated columnar epithelial cell. Foot glands consisted of multicelluear mucous acini were present in the subepithelial connective tissue and muscular layer. The mucous duct of foot glands was distributed from connective tissue to epithelial cells, and open onto the surface of the foot. The foot muscle traveled in three planes as longitudinal, transverse and vertical muscle fiber. About the properties of mucosubstances on the foot, cilia of epithelial cell contained sialomucin only and epithelial cell neutral mucosubstances only. Most of mucous duct within epithelial cells and connective tissue showed a mixture of acidic and neutral mucosubstances, while a few mucous ducts were contained acidic ones only. Among acidic mucosubstances, notable strong sulfomucin were found in most mucous cell of duct, and a mixture of weak sulfomucin and sialomucin appeared in a few cells. The properties of mucosubstances in the food glands were similiar to those of mucous duct. But a few mucous acini contained neutral mucosubstances and sialomucin only in upper and lower portion of foot gland, respectively.

메추라기의 위점막 점액질의 조직화학적 성상에 관한 연구

박선옥,조기진,조운복 부산대학교 사범대학 과학교육연구소 1999 科學敎育硏究報 Vol.26 No.-

메추라기 위 점액분비세포의 점액질 성상을 조직화학적으로 밝히기 위하여, periodic acid Schiff(PAS)염색, alcian blue pH2.5 및 pH1.0염색, aldehyde fuchsin pH1.7 및 pH1.0염색, alcian blue pH2.5 -PAS염색, alcian blue pH1.0-PAS염색 및 aldehyde pH1.7-alcian blue pH2.5염색을 시행하여 관찰한 결과 다음과 같은 결론을 얻었다. 전위 점막상피의 점액질은 체부에서 표면상피는 상당량의 강 sulfomucin과 중성점액질이 혼합된 점액질이었고 관상선상피는 소량 내지 상당량의 강 sulfomucin과 중성점액질의 혼합성이었으나, 사낭 연접부 전위에서 표면 및 관상선상피는 상당량의 강 sulfomucin만을 가진 세포와 상당량의 강 sulfomucin과 소량 또는 상당량의 중성점액질을 가진 세포가 구별되었다. 전위복합선상피의 점액질은 소량 또는 상당량의 강 sulfomucin과 중성점액질의 혼합성이었다. 사낭 점막상피의 점액질은 체부에서 표면상피는 상당량의 강 sulfomucin만을 가진 세포와 상당량의 강 sulfomucin과 미량의 중성점액질을 가진 세포가 구별되었고 전자가 대부분이었으나 사낭선은 소량의 중성점액질을 함유하고 있었으며, 전위 연접부 사낭에서 표면 상피는 상당량의 sulfomucin만을 가진 세포와 소량 또는 상당량의 sulfomucin과 상당량의 중성점액질을 가진 세포가 구별되었으며 사낭선은 미량의 sulfomucin과 소량의 중성점액질의 혼합성이었다. This experiment was performed to study histochemical properties of epithelial mucosubstances in the gastric mucosa of the quail(Coturnix coturnix japonica). The specimens used in this experiment were obtained from the proventriculus and gizzard, of the quail. The removed tissues were fixed in 10% neutral buffered formaline, then were embedded in paraffin and sectioned at 6㎛ . The sections were stained by hematoxylin and eosin for histological structure of proventriculus and gizzard, and by periodic acid Schoff(PAS), alcian blue pH 2.5, alcian blue pH 1.0, aldehyde fuchsin pH 1.7, aldehyde fuchsin pH 1.0, alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS, aldehyde fuchsin pH 1.7-alcian blue pH 2.5, for the histochemical differentiation of mucosubstances, respectively. The results were as follows: In the corpus of proventiculus, the mucosubstances of surface epithelial cells were contained large amounts of strong sulfomucin and neutral mucin, the mucosubstance of the epithelial cells in the tubular gland were small or large amount of strong sulfomucin and neutral mucin. In proventriculus of proventriculogizzard junction, surface epithelium and tubular gland showed two types of cells with mucosubstances, one large amounts strong sulfomucin and small or large amounts of neutral mucin. In composite gland, the mucosubstances of epithelial cells were recognized as consisting of small or large amounts of strong sulfomucin and neutral mucin. In the corpus of gizzard, there were two kinds of cells consisting of surface epithelium in mucosa: one large amounts of strong sulfomucin, the other large amounts of strong sulfomucin and minimal amounts of neutral mucin. The former were much more than the latter in the number of cells. In tubular gland on the gizzard corpus, the mucosubstances of epithelial cells were recognized as consisting of small amounts of neutral mucin. In the gizzard of proventriculogizzard junction, there were two types of cells in surface epithelium: one cell having large amounts of sulfomucin, other cells having large amounts of sulfomucin and neutral mucin. In gizzard gland of above region, it was contained minimal amounts of sulfomucin and small amounts of neutral mucin.

국내육성 오차드그라스 품종들의 캘러스 형성율 및 식물체 재분화 효율

김기용,강경민,배은경,이인애,임용우,최기준,박근제,손대영,조진기 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Comparisons of callus formation ratios from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency for 4 orchardgrass (Dactylis glomerata L.) varieties (Three were developed in Korea and one was imported from foreign nation) are as follows; Jangbeol 102 (67.0%) has the highest callus formation ratio in 4 weeks incubated callus after bedding the seed explants, but Potomac (68.4%) has the highest ratio in 6-seek callus. Potomac (3.93cm) has the highest callus size in 4-week callus, but Jangbeol 101 (4.32cm) has the highest size in 6-week callus. Jangbeol 101 (17.7%) has the highest plant regeneration ratio in 4-week callus, but Potomac (37.4%) has the highest raion in 6-week callus. Jangbeol 102 (11.5%) has the highest plant regeneration efficiency in 4-week callus, but Potomac (25.6%) has the highest efficiency in 6-week callus.

GPS Phone 개발 환경 구축을 위한 PDE Emulator 구현

조진근(Jin Keun Jo),황진옥(Jin-Ok Hwang),민성기(Sung-Gi Min) 한국정보과학회 2004 한국정보과학회 학술발표논문집 Vol.31 No.2Ⅲ

GPS Phone이 Location Service를 제공하기 위해서는 위성데이터를 GPS Phone에 제공하는PDE(Position Determination Entity)가 필요한데 초기 개발단계에서 실제 PDE를 개발단계의 GPS Phone에 연동하기는 번거롭고 비효율적인 면이 많다. 따라서 실제 PDE 중 대신하여 개발단계의 GPS Phone 에 위성데이타를 제공해 줄 SW가 필요하다. 이에 본 논문은 Location Service를 제공하는 GPS Phone 초기 개발단계에서의 시험을 위해 실제 PDE를 대신할 수 있는 PDE Emulator를 구현하였다.

Effects of Non-Specific Expression of Target Gene in Transgenic Pigs Producing Recombinant Human Von Willebrand Factor

Hyun-Gi Lee,In-Sul Hwang,Eun-Mi Ko,Su-Jin Jo,Hee Kyoung Chung,Jin Hyun Kim,Sung Woo Kim,Jin-Ki Park,Soo-Bong Park,Won-Kyong Chang,Hwi-Cheul Lee 한국동물번식학회 2009 Reproductive & Developmental Biology(Supplement) Vol.33 No.2s

Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs

Seul-Gi Yang(Seul-Gi Yang),Jae-Hun Choi(Jae-Hun Choi),Young-Seo Jo(Young-Seo Jo),Ye-Won Kim(Ye-Won Kim),Dong-Mok Lee(Dong-Mok Lee),Hyo-Jin Park(Hyo-Jin Park),Deog-Bon Koo(Deog-Bon Koo) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.2

Various factors in the ovary are known to regulate oocyte maturation and hormone secretory functions; however, the effect of ovarian extract (OE) on oocyte maturation and embryonic development in pigs remains unknown. In this study, we first evaluated whether OE supplementation in the in vitro maturation (IVM) medium alters the oocyte maturation capacity by affecting glucose/amino acid metabolites, meiotic maturation, cumulus cell (CC) expansion, and antioxidants. Various OE concentrations (50, 100, 200, 500, and 5000 μg/mL) were included in the IVM medium. Only the oocytes treated with 100 μg/mL OE exhibited an improved meiotic maturation rate when compared with that of the other groups (non-treated group, 78.6 ± 3.0% vs. 100 μg/mL OE-treated group, 81.6 ± 4.3%); however, the difference was not significant. To observe the changes in glucose and amino acid metabolism in the OE-treated oocytes, we measured the amounts of diverse constituents (glucose, lactate, glutamine, and ammonia) in the IVM medium containing OE. Lactate and ammonia levels in the OE-treated group after 44 h of IVM were higher (p < 0.01) than those in the non-treated group. In addition, the expression of the CC expansion factors (Has2 and Tnfaip6) significantly increased (p < 0.05), whereas the mRNA expression levels of antioxidative enzymes (Sod1, Cat, and Gpx1) significantly diminished (p < 0.05) in the OE-treated group. Moreover, mature oocytes treated with 100 μg/mL OE demonstrated increased subsequent embryonic development rates after 144 h of IVM. Thus, the addition of OE in IVM mediums may improve oocyte maturation capacity which could enhance antioxidative enzyme activation, energy metabolism, and expression of the CC expansion factors in porcine oocytes.

초음파 유도 하 자하거 약침치료로 호전된 후두신경통 환자 1례에 대한 증례보고

홍진기(Jin Gi Hong),선정흠(Jung Hum Sun),하동현(Dong Hyon Ha),김진현(Jin Hyun Kim),서민조(Min Jo Seo),선민지(Min Ji Sun),유성재(Sung Jae Yoo) 대한스포츠한의학회 2023 대한스포츠한의학회지 Vol.23 No.1

간암 환자에서 발생한 위장관 운동장애 및 소장내 세균과증식

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