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NATURAL LANGUAGE SEMANTICS PROCESSING-CASE IDENTIFYING BASED ON MEANING RELATION
Bo, Wang Jian,Zhu, Wang Kai,Yue, Ma,Zhou, Zhou Jing 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
Natural language semantics is the key problem in natural language processing. Information used to analyse natural language semantics comes from several sides. We study meaning relation inter text, paragraph and sehtence, and propose a method to determine setence meaning. Basing on them, we implement a news report understanding system. In our system, meaning representation is in semantic network form based on case relation. Depending on meaning relation, a candidate eliminating method to identify case relation is developed. For each nominal chunk (NC), it can play several case roles. These case roles are NC's case candidates in a sentence. Improper case candidates are eliminated and the proper one is choosed as case role in the sentence.
High-j Proton h11/2 and g7/2 Intruder Bands in 113In
Ma Ke Yan,Lu Jing Bin,Ma Ying Jun,Li Jian,Yang Dong,Sun Wu Ji,Wang Hao,Pan Hao Nan,Wang Jia Qi,Yang Qing Yu,Zhang Da Ming,Zhu Li Hua,Wu Xiao Guang,Zheng Yun,Li Cong Bo 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.76 No.12
Excited states of 113In have been populated through the heavy-ion fusion evaporation reaction 110Pd(7Li, 4n)113In. A new band with the configuration of a proton d5/2 orbital is identified. Two ΔI = 2 intruder bands, built on the πh11/2 and the πg7/2 orbitals, have been extended to spins (63/2-)ħ and (55/2+)ħ, respectively. The negative-parity πh11/2 intruder band shows a smooth increase in aligned spin, which is attributed to a strong proton-neutron interaction. The properties of the positive-parity πg7/2 band are discussed based on tilted axis cranking model calculations, and the features of the antimagnetic rotation for this band are shown after backbend. Furthermore, the contributions of the two-shears-like mechanism, the neutron (gd)ν shell and the core rotation are investigated for the positive-parity πg7/2 band.
Li, Jing-Ping,Cao, Nai-Xia,Jiang, Ri-Ting,He, Shao-Jian,Huang, Tian-Ming,Wu, Bo,Chen, De-Feng,Ma, Ping,Chen, Li,Zhou, Su-Fang,Xie, Xiao-Xun,Luo, Guo-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6
Background: GC-binding factor 2 (GCF2) is a transcriptional regulator that represses transcriptional activity of the epidermal growth factor receptor (EGFR) by binding to a specific GC-rich sequence in the EGFR gene promoter. In addition to this function, GCF2 has also been identified as a tumor-associated antigen and regarded as a potentially valuable serum biomarker for early human hepatocellular carcinoma (HCC) diagnosis. GCF2 is high expressed in most HCC tissues and cell lines including HepG2. This study focused on the influence of GCF2 on cell proliferation and apoptosis in HepG2 cells. Materials and Methods: GCF2 expression at both mRNA and protein levels in HepG2 cells was detected with reverse transcription (RT) PCR and Western blotting, respectively. RNA interference (RNAi) technology was used to knock down GCF2 mRNA and protein expression. Afterwards, cell viability was analyzed with a Cell Counting Kit-8 (CCK-8), and cell apoptosis and caspase 3 activity by flow cytometry and with a Caspase 3 Activity Kit, respectively. Results: Specific down-regulation of GCF2 expression caused cell growth inhibition, and increased apoptosis and caspase 3 activity in HepG2 cells. Conclusions: These primary results suggest that GCF2 may influence cell proliferation and apoptosis in HepG2 cells, and also provides a molecular basis for further investigation into the possible mechanism at proliferation and apoptosis in HCC.
Fu, Bo,Ren, Liang,Liu, Di,Ma, Jian-Zhang,An, Tie-Zhu,Yang, Xiu-Qin,Ma, Hong,Zhang, Dong-Jie,Guo, Zhen-Hua,Guo, Yun-Yun,Zhu, Meng,Bai, Jing Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.12
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB- oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.
Transfer characteristics analysis of bilayer coil structures for wireless power transfer systems
Li, Yang,Ma, Jing-nan,Shi, Shao-bo,Huang, Yue-peng,Wang, Rui,Liu, Jia-ming,Ni, Xin The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.5
Although bilayer coil structures can satisfy the application requirements for inductively coupled power transfer (ICPT) systems in practice, the transfer characteristics of systems (e.g., transfer power, transfer efficiency, and misalignment tolerance) under external interference are seldom studied. In this paper, an equivalent model of a wireless power transfer (WPT) system with a bilayer coils structure was established, and the influences of coil structures on transfer power and efficiency were analyzed. Then, the bilayer coil structures were modeled through multi-physics simulations, the applicable range was determined, and the influences of the coil structures on the transfer performance were studied in detail. Finally, an experiment prototype was set up, and experimental results showed that the coupling coil structures has an obvious effect on the transfer performance. The research in this paper is beneficial for the operation safety and reliability of WPT systems.
Kai Qian,XiaoHai Gong,Bo Guan,SuPing Wu,JingJing Zhang,Jing Qian,YanFei Cai,Yun Chen,ZuoYing Duan,Xin Ma,HuaZhong Li,Jian Jin 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Glucagon-like peptide-1 (GLP-1) was a potential therapeutic drug for type II diabetes, mainly because of the stimulatory effect on insulin secretion under condition of high blood glucose. We used PCR to obtain a recombination gene, GGH, in which two GLP-1 (GLP-1A2G) mutants were connected in series and then fused to the N terminal of human serum albumin. The fusion gene was inserted into pGAPZaA plasmid with Saccharomyces cerevisiae α- factor secretion signal sequence, and was expressed by the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. The engineered strain was constructed by integrating the recombinant plasmid pGAPZαA/GGH into the genome of Pichia pastoris GS115. Genome PCR and western blot showed that the recombinant P. pastoris successfully expressed the fusion protein GGH. The yield of GGH reached 78 mg/L after 72 h fermentation in a flask, using glucose as the optimal carbon source. Fed-batch fermentation was investigated in a 5 L bioreactor, and the expression level of GGH reached 246 mg/L in 52 h. The fusion protein GGH was purified in four steps, and the final purity was 96.1%. The in vitro bioactivity of GGH was the same as that expressed in P. pastoris by the AOX1 promoter. This study described an efficient way to express GGH fusion protein in P. pastoris using GAP promoter, fermentation was easier to control without carbon source change and fermentation time was 20 h less than AOX1 promotercontrolled GGH fermentation.
Azhar Rasul,Muhammad Khan,Bo Yu,Muhammad Ali,Yang Jing Bo,Hong Yang,Tonghui Ma 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.10
Isoalantolactone, a sesquiterpene lactone, possessesanti-fungal as well as cytotoxic properties. In thisstudy, the effects of Isoalantolactone on cell viability, cellcycle, and apoptosis were investigated in human gastricadenocarcinoma SGC-7901 cells. The results demonstratedthat Isoalantolactone induced morphological changes anddecreased cell viability. Subsequently, we found that Isoalantolactoneinduced G2/M and S phase arrest, which wasassociated with a decrease in the expression level of cyclinB1. Apoptosis triggered by Isoalantolactone was visualizedusing propidium iodide (PI) and Annexin V-FITC/PIstaining. Isoalantolactone-induced apoptosis of SGC-7901cells was associated with the dissipation of mitochondrialmembrane potential (DWm) that was due to the down-regulationof Bcl-2 and up-regulation of Bax that led to thecleavage of caspase-3. Additionally, it was found thatIsoalantolactone was involved in the inhibition of phosphorylationof PI3K/Akt. Isoalantolactone-induced cytotoxicityand apoptosis of SGC-7901 cells involvemitochondria-caspase and PI3K/Akt dependent pathways,which gives the rationale for in vivo studies on the utilizationof Isoalantolactone as a potential cancer therapeuticcompound.
Yong Cui,Yong-Bo Song,Lin Ma,Yan-Feng Liu,Guo-Dong Li,Chun-Fu Wu,Jing-Hai Zhang 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.10
This study utilized the E. coli expression system to investigate the role of amino acid residues in toxin from the Chinese scorpion - Buthus martensii Karsch (BmKAS). To evaluate the extent to which residues of the toxin core contribute to its analgesic activity, ten mutants of BmKAS were obtained by PCR. Using site-directed mutagenesis, all of these residues were substituted with different amino acids. This study represents a thorough mapping and elucidation of the epitopes that form the molecular basis of the toxin’s analgesic activity. Our results showed large mutant-dependent differences that emphasize the important roles of the studied residues.