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제주산 양식넙치 ( Paralichthys olivaceus ) 로부터 분리된 β - 용혈성 연쇄구균 ( β - Streptococcus spp. ) 의 특성
허문수(Moon Soo Heo),송춘복(Choon Bok Song),이제희(Je Hee Lee),여인규(In Kyu Yeo),전유진(You Jin Jeon),이정재(Jung Jae Lee),정상철(Sang Chul Chung),이기완(Ki Wan Lee),노섬(Sum Rho),최광식(Kwang Sik Choi),이영돈(Young Don Lee) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4
2000년도 2회에 걸쳐 제주도 일원 넙치 양식장을 대상으로 감염어에서 분리된 연쇄구균에 대한 특성시험 결과를 요약하면 다음과 같다. 감염어로부터 분리된 연쇄구균은 형태적으로 연쇄상의 gram 양성의 구균이었고, catalase와 oxidase에 음성이었으며, 용혈성 시험에서는 β-용혈성을 나타내었다. 감염어에서 각 장기별 생균수에서는 환부, 신장, 혈액 그리고 뇌순으로 연쇄구균이 검출되었다. 약제 감수성에서는 ampicillin, ciprofloxicin 등에 감수성이 있었지만 erythromycin 등에는 감수성이 없었다. 복강에 주사하여 병원성 시험을 한 결과 10일 이내에 전량 폐사하는 강한 병원성을 보였다. 감염균의 용혈성에 대한 특성은 50℃, 30분의 배양에서 용혈활성이 빠르게 증가되어 최대값에 도달되었고 또한 pH 5.5∼6.5에서 높은 활성값을 보였다. Streptococcus spp. of bacterial pathogen of fish were isolated from the cultured flounder (Paralichthys olivaceus) in fish farm of Jeju Island. Clinical signs of the infected flounder which are the most commons symptoms are as follows: erratic swimming, darkening of the body colour, unilateral or bilateral exophthalmia, corneal opacity, hemorrhages in the opercular and the bases of the fins, and the ulceration of the body surface. Biochemical characteristics of pathogenic fish Streptococcus spp. were gram positive, spherical form, catalase negative, oxidase negative and β-haemolytic, respectively. The viable cells counted from the tissue of the diseased flounder were the largest in the order of the ulcer, the kidney, the blood and the brain. The drugs used were ampicillin, ciprofloxacin, doxycycline, gentamycin, tetracycline, erythromycin, streptomycin and oxytetracycline. Streptococcus spp. were found to be sensitive to ampiallin, ciprofloxacin, doxycycline and gentamycin, but were resistant to tetracycline, erythromycin, streptomycin and oxytetracycline. The pathogenicity of Streptococcus spp. on the cultured flounder with an abdominal cavity injection was high. The haemolytic activity of the toxin against the sheep red blood cells reached the maximum after 30 min incubation at 37℃ or 50℃. The toxin showed highest activity at pH 5.5∼6.5.
가미정,김수미,김영경,남수진,오루미,육지수,이혜인,최미나,최혜민,허다은 이화여자대학교 간호과학대학 2010 이화간호학회지 Vol.- No.44
Purpose: The purpose of this descriptive study is to examine the correlation between nursing students’ perception of nurses and the level of their satisfaction with the major. Method: The survey was done with the total number of 192 nursing students from 10 different universities in S city selected by a convenience samplingmethod. In order to measure the perception of nurses, the measurement which was invented by Lee(1992) and revised by Jang(1998) was used. Also, the measurement designed by Shim, and modified by Kim(2007) was used for measuring of the students’ satisfaction in their major. The collected data was analyzed by SPSS 17.0. Results: 1.The most decisive factor of the conceptualization of the image of nurses for the nursing college students is their experience of meeting nurses as a patient. 2. Students reconsider the role of nurses by experiencing immediate relationships with nurses in field training. 3. Other factors of the survey such as the students’academic year, religion, the field training situation (individual experience, type of the hospital where they experienced the training, and a relationship with the nurse in charge of the training) or the existence of nurses among their family members do not affect the students’ satisfaction. 4. There is a positive correlation between the image of nurses of nursing college students and their satisfaction with the major(0.66). Conclusion: The positive image of nurses is directly related to the students’ satisfaction in the nursing major, which hugely affects on their academic achievements and decisions of career path. Therefore, there should be more efforts to increase the positive perception of nurses among nursing students.
Formalin이 넙치(Paralichthys olivaceus) 난 발생 및 부화자어에 미치는 영향
박창범,나오수,이치훈,김병호,이영돈,허문수,이정재,정상철,이기완,노섬,송춘복,최광식,이제희,여인규,전유진 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1
Effects of formalin treatment on embryogenesis and larvae growing in fertilized eggs and hatched larvae of olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solution with 100, 200, 400, 800 and 1,000 ppm of nominal formalin concentration. They were kept in sea water of 16, 18 and 22℃, respectively. Survival rate and hatchability of fertilized eggs were high in control groups more than formalin treated groups in 16, 18 and 22℃, respectively (P<0.05). A similar effect that survival rate of hatched larvae was also observed for control and treatment groups. On the other hand, fertilized eggs treated with 400ppm formalin, were all death in kept in 22℃. These results suggest that high-dose of formalin in fertilized eggs and hatched larvae in olive flounder, Paralichthys olivaceus was inhibited the normal embryogenesis for fertilized eggs and growing for hatched larvae. Also, these inhibited effects was promoted in higher temperature.