[환경] 음용 원수의 염소소독에 의한 소독부산물 생성패턴에 관한 연구 Ⅱ

이강진(Kang Jin Lee1),홍지은(Jee Eun Hong),표희수(Heesoo Pyo),박송자(Song-Ja Park),유제강(Je Kang Yoo),이대운(Dae Woon Lee) 한국분석과학회 2004 분석과학 Vol.17 No.1

위암과 식도암이 병발된 다발성 원발성 악성종양 1예

박사영,이나영,이효진,이선영,최진혁,이순남,심강섭,성순희,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Multiple primary cancer means that more that two cancers occur independently in an individual. Recently, the incidence of multiple primary cancer has increased with lengthened survival, of cancer patients, development of new diagnostic technique and increased clinical evaluation. We report a patient who had adenocarcinoma of stomach combined with squamous cell carcinoma of esophagus simultaneously.

마산만 퇴적물의 중금속 및 유기염화합물 오염에 관한 연구

이찬원,민병윤,권영택,양운진,이규환 경남대학교 환경문제연구소 1990 환경연구 Vol.12 No.-

마산내만 및 마산만 유임 주요하천의 오염퇴적물을 1988년 7월부터 1989년 8월에 걸쳐 채취하여 중금속 및 유기염소화합물을 분석하였다. 오염퇴적충의 뚜께는 10㎝에서 169㎝의 넓은 분포를 보였으며 조사지점의 중금속농도는 지점에 따라 많은 차이를 나타내었다. 특히 인근에 마산수출자유지역 및 조선산업이 위치한 조사 지점에서는 모든 중금속의 농도가 가장 높게 나타났다. 마산만 퇴적오염물 중금속 농도를 오염된 일본의 자료와 비교하였을때 Osaka만의 수온을 제외하고 모든 평균농도가 높게 나타났다. 유기염소화합물(PCB 및 DDE)의 농도는 주거지역 및 공장지대가 함께 위치한 산호천 및 마산시 연안지역에서 높게 검출되었다. 본 연구결과에 의하면 마산만과 같은 폐쇄성 해역에서 중금속 오염원으로 공장폐수가 고려될때는 퇴적된 오염물질의 중금속 분석이 좋은 척도가 철 수 있음을 시사하고 있다. Sediments were collected from Masan bay and two main waterways into Masan Bay in the period of July 1988 to August 1989 for the analysis of heavy metals and Organochlorine compounds. The polluted layer of black sludge had a wide range between 10㎝ to 169㎝ and the content of heavy motels was widely distributed from site to site. One site near the Masan free-export area and a shipmaking company was extremely contaminated by heavy metals. All the average concentration of heavy metals in the sediments from Masan Bay were higher than those from Japanese bays compared except Hg from Osaka Bay. Higher levels of PCBs and DDE were found in sediments from Sanho stream and along the coastal area of Masan city. The results obtained in this study imply that the heavy metal analysis of sediments may be a useful to reveal the pollution sources in a closed bay fuck as Masan Bay.

Canine Distemper Virus 검출을 위한 정량 Real-time PCR

이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.

일측전정기과 손상 희쥐의 전정보상과정에서 뇌간 신경핵의 cAMP-Response Element Binding 단백질 활성화

윤상대,이정헌,김진호,김민선,박병림 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.12

違憲條約의 效力과 司法審査

蘇鎭雲,李商鉉 군산대학교 지역개발연구소 1994 地域開發硏究 Vol.6 No.-

Any discussion of the relationship between international Law and municipal Law must itself with two main problems. First, there is the theoretical question of whether international Law and municipal Law are part of a universal legal order(monism), or form two distinct systems of Law(dualism). Secondly, a court may be faced with the practical difficulty of reaching a decision where there exists a conflict between the rules of international Law and the rules of municipal Law relevant to the particular sitution. The object of the present discussion is to ascertain in what manner and to what extent municipal courts do apply a rule of international Law. A further question is, how far a rule of municipal Law will be applied by a municipal court if is a ctually conflicts with a rule of municipal Law judge-made statutory rule. The answers to these questions will be found to require distinctions to be made, on the one hand, between customary and treaty rules of international Law; and on the other between statutory and judge-made municipal Law. Based on this premise, this study atempts to clarify the relationship between treaties and constitution focused on the formality of concluding treaties and the effect. Common throughout the constutional provisions and constitutional traditions of various nation is their recognition of the national validity of treaties. Conflicts between treaties and constitutional involve both discrepancies in contents and conclusion procedures that run counter to the constiution. The constitutional system of modern states seeks to achieve harmony in contents between the two statutroy entities under the principle of public Law unity. In practice, too, the two statutroy entities are being adjusted to another from the viewpoint of respecting treaties and increasing trends twoard internationalism.

정상과 만성염증성 활막배양세포에서 Corticotropin releasing hormone(CRH)이 cyclooxygenaase-1(COX-1)과 cyclooxygenase-2(COX-2) mRNA발현에 미치는 영향

정운원,이승관,이창규,조경진,김성욱,하주희,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2000 保健科學論集 Vol.26 No.1

Corticotropin-releasing hormone(CRH) is a major regulator of the hypothalamic-pituitaryadrenal axis(HPA). In inflammatory stress, the cytokines TNF-, IL-6 stimulate the production of CRH, a 41 amino acids neuropeptide, in the hypothalamus. The release of CRH leads to pituitary production of adrenocorticotropic hormone, followed by glucocorticoid secretion by the adrenal cortex. Glucocorticoids suppress namy components of the inflammatory process. Recently, CRH and CRH receptor were reported to be located in the periphery such as Immune system and chronic inflammatory sites as rheumatoid arthritis (RA). Cyclooxygenase consisted of two isoforms, COX-1 and COX-2, converts arachdonate to prostaglandins(PGs) which are important mediators of inflammation. insymoviocyte in RA, it was described that COX-2 mRNA was markedly increased by inflammatory agents, PMA or IL-1 and COX-1 transcripts were not modulated. We examined the modulation of COX by immune CRH in cultured normal and rheumatoid synoviocytes. Our results were shown that COX-1 mRNA expression decreased with the each stimulation of PMA and IL-1 in normal synoviocyte. In RA synoviocyte, PMA and IL-1 were increased mRNA expression of COX-1. In simultaneous treatment with CRH, PMA group was decreased, but IL-1 group was increased mRNA expression of COX-1. COX-2 mRNA expression was slightly increased by the treatment with PMA and highly increased by IL-1. After CRH treatment, PMA and IL-1 addictively increased COX-2 mRNA expression. We think that these results are contributed to the influence of increased cANP by CRH on the promoter of COX-2 in normal and synoviocytes. Because many cytokines, neuropeptides, and signal transduction pathways are involved in chronic inflammation, the exact role of CRH on inflammation is not fully elucidated. To achieve this goal, further experiments are needed.

憲法訴願制度에 관한 硏究

蘇鎭雲,李商鉉 군산대학교 지역개발연구소 1991 地域開發硏究 Vol.3 No.-

남강에 적용된 하천 수질모델의 비교 연구

김봉진,윤기섭,양운진,이찬원 경남대학교 환경문제연구소 1995 환경연구 Vol.17 No.-

The water quality models were applied to the Nam river The Nam river is one of the major tributaries of the Nakdong river. The entire stretch of 60 ㎞ was divided into 16 reaches. The models were calibrated and verified to the field data. The predicted water qualify showed good agreements with the field measurements by two models. However, the STREAM(Segment Travel River Ecosystem Autograph Model) model was approved to be better· than QUAL 2E model for the Nam river. It may be due to that the dissolved oxygen changes in water bodies are better by STREAM than by QUAL 2E model. From the results, the water quality of the Nam river was affected by the input loadings from the Haman stream. The shortness of the distance from this point to the Nakdong river made the joining water quality to the Nakdong river limit the purification capacity.

기름골의 품질관련 특성 평가 : Cyperus esculentus L., var sativus Boeck

송진,방진기,박희운,이승은,박남규 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.4

기름골의 일반적인 이화학성분과 기름을 추출하여 기름의 특성을 평가한 결과를 요약하면 다음과 같다. 1. 기름골의 일반성분 중 조지방과 조섬유의 함량은 각각 22.4%, 13.3%이고, 총 아미노산 함량은 6,882.4㎎%로 cystein과 isoleucine의 함량이 1,572㎎%, 905㎎%로 가장 높았다. 2. 기름골 전분의 아밀로스 함량은 46.9%였고, RNA 측정결과 최대, 최저 및 최종점도는 각각 451, 280 및 375RNA였다. 3. 기름골 기름의 산가와 과산화물가는 2.1, 6.2meq/㎏이며 지방산 함량은 oleic acid가 70%를 차지하였다. 4. 기름골 기름의 토코페롤 측정결과 α, β, γ, δ가 모두 검출되었고, 그 중 α-tocopherol이 약 15㎎%로 가장 많이 함유되었다. 5. GC/MS를 이용하여 향기성분을 분석한 결과 주된 향기성분은 2.5dimethyl pyrazine으로 약30%를 차지하였다. The objective of this study were to evaluate quality characteristics of the chufa tuber and its oil. The results are summarized as follows : Crude fat, crude fiber and total amino acid were 22.4%, 13.3% and 6.882㎎% of the chufa tuber, respectively. Amylose content of chufa starch was 46.9% and maxium. Minimum and final viscosity of amylogram characteristics in chufa starch were 451, 280 and 375 RVA, respectively. The acid value of oil was 2.1 and peroxide value was 6.2meq/㎏. The content of oleic acid, a major fatty acid in chufa oil, was 70%. Four type of tocopherol were detected and α-tocopheral was shown to be the major tocopherol. The volatile component was measured by GC/MS. 2.5-Dimethyl pyrazine was the dominant volatile component in the whole chufa oil.