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Shin, Jihye,Kim, Hye-Jung,Kim, Gamin,Song, Meiying,Woo, Se Joon,Lee, Seung-Taek,Kim, Hoguen,Lee, Cheolju American Chemical Society 2014 JOURNAL OF PROTEOME RESEARCH Vol.13 No.11
<P>To discover serological colorectal cancer (CRC) markers, we analyzed cell line secretome to gather proteins of higher potential to be secreted from tissues into circulation. A total of 898 human proteins were identified, of which 62.2% were predicted to be released or shed from cells. The identified proteins were compared with tissue proteomes to find candidate proteins whose expressions were elevated in tumor tissues compared with normal tissues as revealed by (i) quantitative proteomic analysis based on cICAT and mTRAQ or (ii) data mining of immunohistochemical images piled in Human Protein Atlas database. By applying various stringent criteria, 11 candidate proteins were selected. Among these, we validated an significant increase (<I>p</I> = 0.0018) of melanotransferrin (TRFM) at the plasma level of CRC patients through Western blotting, using 130 plasma samples containing 30 healthy controls, 80 CRC patients, and 20 patients of other diseases. Finally, we measured the expression level of TRFM in 325 plasma samples containing 77 healthy controls and 228 CRC patients (34.6 ± 4.2 ng/mL and 67.0 ± 6.4 ng/mL, <I>p</I> < 0.0001) through ELISA and demonstrated the area under the receiver operating characteristic curve of 0.723 (<I>p</I> < 0.0001) with a 92.5% specificity, 48.2% sensitivity, and 95.7% positive predictive value. Furthermore, unlike CEA and PAI-1, up-regulation of TRFM in pathological stages I & II groups compared with stages III & IV groups lead us to expect the use TRFM for early-stage diagnosis of CRC. In this study, we suggest TRFM as a potential serological marker for CRC and expect our discovery strategy to help identify highly cancer-specific and body-fluid-accessible biomarkers.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2014/jprobs.2014.13.issue-11/pr500790f/production/images/medium/pr-2014-00790f_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/pr500790f'>ACS Electronic Supporting Info</A></P>
Comparison of L1 and L2 Reading Strategy Use of Korean English Learners
Jihye Shin,Boon-Joo Park 한국영미어문학회 2013 영미어문학 Vol.- No.109
This study investigated similarities and differences between L1 and L2 reading strategies of Korean English learners. Twenty-one Korean university students participated in the study. Data was obtained by means of the think-aloud method to identify their L1 and L2 comprehension strategies and their patterns focusing on global, support, and problem-solving strategies. In addition, changes in their strategic behaviors as their reading proficiency develops are further explored. The results revealed overall similarities in the basic trends of sub-strategy use in both L1 and L2 reading but differences in the frequency of strategies used. The findings seem unable to be explicated by the linguistic interdependence hypothesis or the linguistic threshold hypothesis alone. Pedagogical implications for strategy training and raising students' metacognitive awareness are suggested.
Shin, Jihye,Kim, Gamin,Lee, Jong Won,Lee, Ji Eun,Kim, Yoo Seok,Yu, Jong‐,Han,Lee, Seung‐,Taek,Ahn, Sei Hyun,Kim, Hoguen,Lee, Cheolju John Wiley and Sons Inc. 2016 CANCER SCIENCE Vol.107 No.6
<P>Cancer cell secretomes are considered a potential source for the discovery of cancer markers. In this study, the secretomes of four breast cancer (BC) cell lines (Hs578T, MCF‐7, MDA‐MB‐231, and SK‐BR‐3) were profiled with liquid chromatography–tandem mass spectrometry analysis. A total of 1410 proteins were identified with less than 1% false discovery rate, of which approximately 55% (796 proteins) were predicted to be secreted from cells. To find BC‐specific proteins among the secreted proteins, data of immunohistochemical staining compiled in the Human Protein Atlas were investigated by comparing the data of BC tissues with those of normal tissues. By applying various criteria, including higher expression level in BC tissues, higher predicted potential of secretion, and sufficient number of tandem mass spectra, 12 biomarker candidate proteins including ganglioside GM2 activator (GM2A) were selected for confirmation. Western blot analysis and ELISA for plasma samples of healthy controls and BC patients revealed elevation of GM2A in BC patients, especially those who were estrogen receptor‐negative. Additionally, siRNA‐mediated knockdown of GM2A in BC cells decreased migration <I>in vitro</I>, whereas the overexpression of GM2A led to an increase in cell migration. Although GM2A as a diagnostic and prognostic marker in BC should be carefully verified further, this study has established the potential role of GM2A in BC progression.</P>
Associations among plasma vitamin C, epidermal ceramide and clinical severity of atopic dermatitis
Jihye Shin,You Jin Kim,Oran Kwon,Nack-In Kim,Yunhi Cho 대한지역사회영양학회 2016 Nutrition Research and Practice Vol.10 No.4
BACKGROUND/OBJECTIVES: Atopic dermatitis (AD), a chronic inflammatory skin disease, is accompanied by disruption of the epidermal lipid barrier, of which ceramide (Cer) is the major component. Recently it was reported that vitamin C is essential for de novo synthesis of Cer in the epidermis and that the level of vitamin C in plasma is decreased in AD. The objective of this study was to determine the associations among clinical severity, vitamin C in either plasma or epidermis, and Cer in the epidermis of patients with AD. SUBJECTS/METHODS: A total of 17 patients (11 male and 6 female) aged 20-42 years were enrolled. The clinical severity of AD was assessed according to the SCORAD (SCORing Atopic Dermatitis) system. Levels of vitamin C were determined in plasma and biopsies of lesional epidermis. Levels of epidermal lipids, including Cer, were determined from tape-stripped lesional epidermis. RESULTS: The clinical severity of patients ranged between 0.1 and 45 (mild to severe AD) based on the SCORAD system. As the SCORAD score increased, the level of vitamin C in the plasma, but not in the epidermis, decreased, and levels of total Cer and Cer2, the major Cer species in the epidermis, also decreased. There was also a positive association between level of vitamin C in the plasma and level of total Cer in the epidermis. However, levels of epidermal total lipids including triglyceride, cholesterol, and free fatty acid (FFA) were not associated with either SCORAD score or level of vitamin C in the plasma of all subjects. CONCLUSIONS: As the clinical severity of AD increased, level of vitamin C in the plasma and level of epidermal Cer decreased, and there was a positive association between these two parameters, implying associations among plasma vitamin C, epidermal Cer, and the clinical severity of AD.