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Lim, Seong-in,Choe, SeEun,Kim, Ki-Sun,Jeoung, Hye-Young,Cha, Ra Mi,Park, Gil-Soon,Shin, Jihye,Park, Gyu-Nam,Cho, In-Soo,Song, Jae-Young,Hyun, Bang-Hun,Park, Bong-Kyun,An, Dong-Jun Elsevier Ltd. 2019 Vaccine Vol.37 No.27
<P><B>Abstract</B></P> <P>Here, we constructed an attenuated live marker classical swine fever (CSF) vaccine (Flc-LOM-BE<SUP>rns</SUP>) to eradicate CSF. This was done by taking infectious clone Flc-LOM, which is based on an attenuated live CSF vaccine virus (LOM strain), and removing the full-length classical swine fever virus (CSFV) E<SUP>rns</SUP> sequences and the 3′ end (52 base pairs) of the CSFV capsid. These regions were substituted with the full-length bovine viral diarrhoea virus (BVDV) E<SUP>rns</SUP> gene sequence and the 3′ end (52 base pairs) of the BVDV capsid gene. Sows were vaccinated with the Flc-LOM-BE<SUP>rns</SUP> vaccine 3 weeks before insemination and then challenged with virulent CSFV at the early, mid- or late stages of pregnancy. We then examined transplacental transmission to the foetuses. Piglets born to sows vaccinated with Flc-LOM-BE<SUP>rns</SUP> did not show vertical infection, regardless of challenge time. In addition, CSFV challenge did not affect the delivery date, weight or length of the foetus. Pregnant sows inoculated with the Flc-LOM-BE<SUP>rns</SUP> vaccine were anti-CSF E<SUP>rns</SUP> antibody-negative and anti-BVDV E<SUP>rns</SUP> antibody-positive. Challenge of pregnant sows with virulent CSFV resulted in anti-CSF E<SUP>rns</SUP> antibody positivity. These results strongly indicate that differential diagnosis can be conducted between the Flc-LOM-BE<SUP>rns</SUP> vaccinated animal and virulent CSFV affected animal by detecting antibody against BVDV E<SUP>rns</SUP> or CSF E<SUP>rns</SUP> gene. Therefore, the Flc-LOM-BE<SUP>rns</SUP> vaccine may fulfil the function of differential diagnosis which required for DIVA vaccine.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The Flc-LOM-BE<SUP>rns</SUP> is live attenuated DIVA vaccine for CSFV. </LI> <LI> The Flc-LOM-BE<SUP>rns</SUP> vaccine protected foetuses from vertical transmission. </LI> <LI> The Flc-LOM-BE<SUP>rns</SUP> vaccine enables differential identification of serum antibodies. </LI> </UL> </P>
Evolutionary dynamics of classical swine fever virus in South Korea: 1987–2017
An, Dong-Jun,Lim, Seong-in,Choe, SeEun,Kim, Ki-Sun,Cha, Ra Mi,Cho, In-Soo,Song, Jae-Young,Hyun, Bang-Hun,Park, Bong-Kyun Elsevier 2018 Veterinary microbiology Vol.225 No.-
<P><B>Abstract</B></P> <P>The 5′ UTR (n=102) and full-length E2 (n=37) genes of classical swine fever viruses (CSFVs) circulating in South Korea over the past 30 years (1987–2017) were examined to determine the evolutionary rate and estimated time of the most recent common ancestor (tMRCA). From 2000, the Korean classical swine fever (CSF) antigen changed from genotype 3 to 2, which comprises subgenotypes 2.1b (2002–2013) and 2.1d (2011–2017). There are genotypic variations in the full-length E2 gene of Korean CSFV genotypes 2.1b and 2.1d (seven separate amino acid substitutions); these are useful distinguishing markers. The mean substitution rate (×10<SUP>3</SUP> substitutions/site/year) for Korean CSFV was estimated to be 2.2088 (95% highest posterior density (HPD): lower, 1.7045; upper, 2.7574) and the mean tMRCA was estimated to be 1901 (95% HPD: lower, 1865; upper, 1933). The effective population size of Korean CSFV genotype 2 increased rapidly from 2002 to 2003, after which it remained constant. The occurrence of CSF in Korea is expected to decline in the future; however, it will likely be more prevalent in wild boar than in domestic pigs. Thus, there is a risk of transmission from wild boar to breeding pigs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> From 2000, Korean CSFV changed from genotype 3 to 2 (subgenotypes 2.1b and 2.1d). </LI> <LI> Subgenotype 2.1d(prevalent in Korea) was detected in wild boar and breeding pigs. </LI> <LI> The mean substitution rate for circulating Korean CSFVs is 2.2114(×10<SUP>−3</SUP> s/s/y). </LI> </UL> </P>
First detection of Influenza D virus infection in cattle and pigs in the Republic of Korea in 2022
Eui Hyeon Lim,Seong-In Lim,Min Ji Kim,MiJung Kwon,Min-Ji Kim,Kwan-Bok Lee,SeEun Choe,Dong-Jun Ahn,Bang-hun Hyun,Jee-Yong Park,You-Chan Bae,Hye-Young Jeoung,Kyung-Ki Lee,Yoon-Hee Lee 한국예방수의학회 2023 한국예방수의학회 학술대회자료집 Vol.2023 No.-
A novel strain of porcine deltacoronavirus in Vietnam
Le, Van Phan,Song, Sok,An, Byung-Hyun,Park, Gyu-Nam,Pham, Ngoc Thach,Le, Dinh Quyen,Nguyen, Van Tam,Vu, Thi Thu Hang,Kim, Ki-Sun,Choe, SeEun,An, Dong-Jun Springer-Verlag 2018 Archives of virology Vol.163 No.1
<P>Two porcine deltacoronavirus (PDCoV) strains (Binh21 and HaNoi6) were isolated from two pig farms in North Vietnam. Phylogenetic analysis of the complete genomes and the Spike and Membrane genes revealed that the two Vietnam PDCoVs belong to the same lineage as PDCoVs from Thailand and Laos; however, the N genes belonged to the same lineage as PDCoVs from the USA, Korea, China, and Hong Kong. The recombination detection program subsequently identified the major parent (S5011 strain) and minor parent (HKU15-44 strain) of the two Vietnam PDCoV strains (p < 0.01).</P>