The Effect of Rosiglitazone on the Cell Proliferation and the Expressions of p27 and Skp2 in<i>Helicobacter pylori</i>Infected Human Gastric Epithelial Cells

Kim, Sung-Soo,Cho, Young-Seok,Kim, Hyung-Keun,Shin, Ok-Ran,Chae, Hiun-Suk,Choi, Myung-Gyu,Chung, In-Sik The Korean Society of Gastroenterology 2010 대한소화기학회지 Vol.55 No.4

<P>Background/Aims: Ligands for peroxisome proliferator-activated receptorgamma (PPARgamma), a member of the ligand-activated nuclear receptor superfamily, exhibit anti-tumoral effects and are associated with de novo synthesis of proteins involved in regulating the cell cycle and cell survival/death. Helicobacter pylori (H. pylori) is an etiologic agent for gastric adenocarcinoma, and raises the cell turnover of gastric epithelium. The aim of this study was to investigate the effect of PPARgamma ligand rosiglitazone on the cell proliferation and the expressions of p27 and Skp2 protein in H. pylori infected gastric epithelial cells. Methods: We examined the expression of PPARgamma by Western blot in H. pylori infected AGS human gastric epithelial cells. The effect of rosiglitazone on the survival of H. pylori infected AGS cells was assessed by cell viability assay. After the treatment of rosiglitazone in H. pylori infected AGS cells, the expressions of p27 and Skp2 were assessed by Western blot. Results: The expression of PPARgamma protein was increased in H. pylori infected AGS cells. Cell growth was inhibited and decreased in dose- and time- dependent manner in H. pylori infected AGS cells treated with rosiglitazone. A decrease in Skp2 expression and a reciprocal increase in p27 expression were found in dose- and time-dependent manner in H. pylori infected AGS cells treated with rosiglitazone. Conclusions: Rosiglitazone inhibited the growth of H. pylori infected AGS cells. Rosiglitazone attenuated Skp2 expression, thereby promoting p27 accumulation in H. pylori infected human gastric epithelial cells. Further studies will be needed to find the effects of accumulation on cell turnover in H. pylori infection and the role in the H. pylori-associated gastric carcinogenesis.</P>

전기화상후 Prostaglandin F2a와 Thromboxane B2의 증가에 대한 Flurbiprofen, Prednisolone 및 Gabexate mesilate의 억제효과

조병채,김인규,변진석,백봉수 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.1

This study was conducted to clarify the inhibitory effect of flurbiprofen, prednisolone, and gabexate mesilate on the synthesis of prostaglandin F2a and thromboxane B2 in the tissue of electrical burn. one hundred sixty Sprague-Dawley rats, 250gm of body weight in average, were divided into 4 groups of control, flurbiprofen, prednisolone and gabexate mesilate administred group. Fourty rats of the each group were subdivided into 1st, 2nd, 4th, 7th postburn day with 10 rats in each subgroup. Ten rats were used for normal values of prostaglandin F2a and thromboxane B2. Flurbiprodfen 30mg/kg was given per mouth, and prednisolone acetate 5mg/kg and gabaxate mesilate 50mg/kg were administred intramuscularly 5 minutes after burn injury and then once a day. All but bone tissue of within 2cm area of the current entry were harvested and ground into homogenate. The levels of prostaglandin F2a and thromoboxane B2 were measured by Radioimmunoassay. The results were as followings: In normal rat tissue, the level of prostagland F2a and thromboxane B2 were 15.8±1.8 and 17.6±2.8ng/mg protein in each. In the control group the levels of both prostaglandin F2a and thromboxane B2 increased abruptly to 5 times and 7 thimes in the first day and maintained the same level in the second day. After then they rapidly decreased and returned to a near normal level in the fourth day. The increase of thromboxane B2 was more remarkable than that of prostaglandin F2a. In the flurbiprofen group, the level of thromboxane B2 was not inerease but kept at a normal level. The average level of prostaglandin F2a in the post-burn first and second day was 59.5% lower than of the control group. The average level on the post-burn first and second day was 61% lower in thromboxane B2 and 33% lower in prostaglandin F2a in the prednisolone group, and 68% lower in throboxane B2 and 48% lower in prostaglandin F2a in the gabexate mesilate group, compared to the control group. These findings suggest that electrically injured tissue is vigrously progressive due to rapid increase of thromboxane B2 and prostaglandin F2a, as inflammatory mediators, in the post-burn first and second day, is nonprogressive after the post-burn fourth day. The flurbiprodfen was the most effective of the three drugs(flurbiprofen, gabexate mesilate, prednisolone in order of effectiveness) to suppress the synthesis of thromboxane B2 prostaglandin F2a of the electrieally injured tissue.

그림 어휘력 검사를 통한 정신지체아동의 수용어휘 발달 수준 탐색

조인수,시옥희 한국정신지체아교육학회 2000 지적장애연구 Vol.2 No.-

정신지체아동과 일반아동의 수용 어휘 능력을 그림 어휘력 검사를 통하여 비교 분석해 봄으로써 언어 특성에 맞는 효과적인 교수 전략과 교육 대책을 마련하여 학습 지도의 기초 자료로 활용하고자 하는 것이 본 연구의 목적이다. 본 연구의 대상인 정신지체아동의 정신지체학교에 재학하고 있는 초등부학생과 특수학급에 재학하고 있는 학생 20명을 선정하였고, 일반아동은 어린이집 유치원, 초등학교에서 생활연령이 정신지체아동과 유사한 아동으로 20명씩 표집하였다. 연구결과 정신지체아동의 수용언어 발달수준에서 정신연령이 증가함에 따라 수용 어휘력은 향상을 보이나 일반 아동에 비해 점차 뒤떨어지며, 이에 반해 일반아동은 연령이 증가할수록 어휘력이 향상되는 것으로 나타났다. 또한 두 집단간의 수용언어 발달수준의 차이는 같은 연령의 경우 모든 연령대에서 정신지체아동과 일반아동간에 차이가 없었다. 그러나 원점수와 백분위점수에서는 3세의 경우 차이가 있는 것으로 나타났으며 정신지체아동이 다소 높은 것으로 나타났다. 즉 이 결과는 정신지체아동의 교육에 있어 비록 정신연령이 낮더라도 생활연령을 고려한 학습 장면의 구성이 필요함을 의미한다. It is important to prepare an effective teaching strategy suitable the language feature and consider an educational counterplan for basic material of teaching plan by means of a comparative analysis about the receptive vocabulary capacity of the mentally retarded children and normal children. The purpose of this study is to define the development features of the mentally retarded children and normal children as for their receptive vocabulary according to age through a picture vocabulary test. The method of this study is to let each 20 mentally retarded children and nomal children whose mental age is 3,5,8 test their vocabulary ability. After putting out the original score, it can be found the age equivalent, percentile score according to a calculated list. And in this study, the results are summarized as the following. Firstly, in receptive vocabulary ability, the receptive language of the mentally retarded children develops as much as their mental age increases but it is inferior to the normal children gradually. As regarding mental age, chronological age makes little difference. And the higher his age is, the higher original score and age equivalent is. But percentile score shows a tendency to decrease. Compared by age, children whose mental age are high make high score in original score and age equivalent but regarding the percentile score, children of 3 years old show a tendency to make higher score than 8 years old. That chronological age makes little difference though the mental age increases means that individual difference among the children is wide. Also it is said that we should consider this fact when we constitute the learning environment. And a percentile score shows a relative position so in the case of mentally retarded children, the fact that as their age increases percentile score shows a tendency to decreases tells that their receptive vocabulary ability is falling behind comparing normal children of the age. Secondly, in the case of normal children, the more their age is, the higher their receptive vocabulary rises. As their mental age increases, statistically original score and age equivalent makes a wide difference but percentile score does not. That tells a stable tendency compared with whole children. Compared among each age, the higher their mental age increases, the higher their chronological age increases and original score and age equivalent do, too. Thirdly, on whole age, the development level of receptive vocabulary between mentally retarded children and normal children makes no difference but in the case of original score and percentile score, the children of 3 years old make a difference and mentally retarded children reach a little higher score. The fact that the mentally retarded children of 3 years old reach higher in the original score and percentile score than normal children and then gradually make a low score as they grow older means the limitations of the mentally retarded children's receptive language. In comparison among the groups, the fact that the mentally retarded children of 3 years old reach a little higher score shows, in the case of the mentally retarded children, can be put out by means of testing instruments and that they can recognize things to that degree. And in special school, minimum students have higher chronological age by 10 years because they have more learning experience than general children. This result requires that we should constitute the learning environment considered their chronological age even though their mental age is low level.

신원방우황청심원의 심혈관계에 관한 약효

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate the phamacological properties of New Wonbang Woohwangchungsimwon Pill (NSCH), effects of Wonbang Woohwangchungsimwon Pill (SCH) and NSCH were compared using various experimental models. In rat aorta, NSCH and SCH made the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6 M) regardless to endothelium containing or denuded rings of the rat aorta. Furthermore, the presence of the inhibitors of NO synthase and guanylate cyclase did not aftect significantly the relaxing effects of NSCH and SCH, NSCH and SCH inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs). NSCH and SCH decreased significantly heart rate. These, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In guinea-pig papillary muscle, these had no effects on parameters of action potential such as action potential amplitude (APA). V_max and resting membrane potential (RMP) at low doses, whereas inhibited the cardiac contractility at high doses. Furthermore, these had a significant inhibitory effects on palpitation of the heart in normotensive rats and SHRs. These results suggest that NSCH and SCH have weak cardiovascular effects, and that there is no significant differences between cardiovascular effects of two preparations.

신우황청심원액의 심혈관계에 관한 약효연구

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-

In order to investigate the pharmacological properties of New Woohwangchungsimwon Liquid (NCL), efects of Woohwangchungsimwon Liquid (CL) and NCL were compared. In isolated rat aorta, NCL and CL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^6M) without regard to intact endothelium or denuded rings of the rat aorta. Furthemore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxation of NCL and CL. NCL and CL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dosedependent manner. In conscious spontaneously hypertensive rats (SHRs), NCL and CL significantly decreased heart rate. NCL and CL, at high doses, had a negative inotropic effect that was a decrease of LVDP and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NCL and CL had no efects on parameters of action potential at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NCL and CL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between two preparations.

알코올에 의한 α-Tocopherol이 혈액성분 및 간조직에 미치는 영향에 관한 연구

조만희,안인수 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

This study was done in order to observe the effects of ethanol and a-tocopherol on the HDL-chelesterol, r-GTP, CPK, aminotransferase(AST-ACT) and histopathologic findings. The white Sprague Dawley rat were Classified into 4 groups:0.9% NaCl ad-ministration was group A 25% ethanol administration for the each 5,10 and 15 days was ethanol group B(BI, B2 and B3), 25% ethanol administration for th each 5 and 10 days, and 50% a-tocopherol for the 2 and 7 days administration was group C(CI and C2), 50% a-tocopherol pretreated for the each 5, 10 and 16 days and 25% ethanol for the 2,7 and 10 days administration was group D(D1, D2 and D3). The following results were obtained 1. The Biochemical changes of serum HDL-cholesterol, r-GTP, CPK, GOT and GPT activities was significantly increased in ethanol treated group than group D.(D1, D2 and D3). 2. The Biochemical changes of each subgroup in ethanol treated group HDL-cholesterol was most highly increased. The group C1 and DI was near normal. 3. The change of r-GTP activity of each subgroup in ethanol treated group was highly increased than the group B1, The Cl and Dl groups was near normal. 4. The change of CPK activity of each subgroup in ethanol treated group was most highly in creased than the B3 group. the group Cl and Dl groups was near normal. 5. The change of GOT and GPT activities of each subgroups in B3 and D3 was most highly increased but the C2 and D2 was not significant. 6. The histopathological changes of ethanol treated group(B2, and B3) found the lipid droplet and infiltration of inflammatory cells, and mild ballooning degeneration. The group C3 and D3 found the mild infiltration and lipid droplet.

Spontaneous Pt Deposition on Defective Surfaces of In₂O₃ Nanocrystals Confined within Cavities of Hollow Silica Nanoshells: Pt Catalyst-Modified ITO Electrode with Enhanced ECL Performance

Cho, Young Shin,Kim, Soo Min,Ju, Youngwon,Kim, Junghoon,Jeon, Ki-Wan,Cho, Seung Hwan,Kim, Joohoon,Lee, In Su American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.24

<P>Although the deposition of metallic domains on a preformed semiconductor nanocrystal provides an effective pathway to access diverse hybrid nanocrystals with synergistic In2O3 metal/semiconductor heterojunction interface, those reactions that take place on the surface of semiconductor nanoscrystals have not been investigated thoroughly, because of the impediments caused by the surface-capping organic surfactants. By exploiting the interfacial reactions occurring between the solution and nanoparticles confined with the cavities of hollow nanoparticles, we propose a novel nanospace-confined strategy for assessing the innate reactivity of surfaces of inorganic semiconductor nanoparticles. This strategy was adopted to investigate the newly discovered process of spontaneous Pt deposition on In2O3 nanocrystals. Through an in-depth examination involving varying key reaction parameters, the Pt deposition process was identified to be templated by the defective In2O3 surface via a unique redox process involving the oxygen vacancies in the In2O3 lattice, whose density can be controlled by high-temperature annealing. The product of the Pt-deposition reaction inside the hollow silica nanoparticle, bearing In2O3-supported Pt catalysts inside the cavity protected by a porous silica shell, was proved to be an effective nanoreactor system which selectively and sustainably catalyzed the reduction reaction of small-sized aromatic nitro-compounds. Moreover, the surfactant-free and electroless Pt deposition protocol, which was devised based on the surface chemistry of the In2O3 nanoparticles, was successfully employed to fabricate Pt-catalyst-modified ITO electrodes.with enhanced electrogenerated chemiluminescece (ECL) performance.</P>

일부섬유업체 근로자들의 우울상태에 관한 연구

한인수,김수영,조영채 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

In order to investigate the relationship among sociodemographic and job related factors, health practices and depressive mood, this survey was performed by self-reported questionnaire on 376 textile industrial workers from August to September 1994, which included items on 8 health practice and Zung Self-rating Depression scale(Zung SDS). The results were summarized as follows ; 1 The Zung SDS scores were higher in female(50.02±6.47) and blue color(48.88±6.55) than male(46.73±6.85) and white color(46.06±7.56) and it was very significantly difference by Wilcoxon test. 2. The severity of depression mood were 9.0% of normal(20∼39 SDS), 33.2% of mild(40∼47 SDS), 45.3% of moderate(48∼55 SDS) and 12.5% of severe(56 and more), respectively. 3. The Zung SDS scores according to the demographic variables were found to be significantly high score in the low educational level group in male, but it was high score in 20 or less age group, unmarried group and low educational level group than others in female. 4. The Zung SDS scores according to the job related variables were found to be significantly high scores in the shift work group, the longer overtime work group and dissatisfaction group to the job satisfaction in female, but it was not significantly difference in male. 5. The Zung SDS scores according to the Health practice Items were found to be significantly related to low depressive socre in male as following 3 items : ① habitual physical activity ② moderate alcohol consumption ③ obesity 10.0∼19.9%. The following 4 items were found in female to be significantly related to low depressive score : ① sleeping regularly 8 hours or more per day ② eating breakfast and snacking regularly ③ no smoking ④ obesity -9.9∼9.9% 6. The Zung SDS Scores were decreased according to increasing the health practice index in sex and job contents.

정신지체 특수학교 전공과 및 전환교육의 운영실태와 개선방향

조인수,도금숙,전보성 한국특수교육문제연구소 2001 특수교육저널 : 이론과 실천 Vol.2 No.1

본 연구의 목적은 정신지체 특수학교의 전공과 및 전환교육의 운영실태와 개선방향을 탐색하는데 주안점을 두었다. 전공과 및 전환교육의 운영실태는 16개 영역으로 조사하고 문제점과 개선방향은 10개 영역을 중심으로 세부적인 내용을 조사 분석하였다. 여기서 조사분석된 내용은 정신지체 직업 · 전환교육에 좋은 지침이 될 수 있을 것이며 타장애영역 학생들의 직업진로 및 전환교육에 실질적인 도움을 줄 수 있을 것이다. The aims of this study are finding out the reality of the operation of the majored subjects and problems and finding out the improved methods about them. And 1 surveyed special teachers who are attending 17 special schools in Korea in which have the majored subjects about the reality of operating them and improved methods about them. First, about surveying the reality of operation, as to the majored subjects, they are mainly teaching students in the form of work training, guiding job, preparing job and daily Iiving skills through classes. The selection of majored subjects conceming jobs are made through school's direction and the cooperation among teachers, students and parents, and teaching jobs are now changed or will be changed because of the maladjustment to student' s aptitude, the assessment on their job aptitude is not being made now. There are many qualified teachers who have the certification in special school and they think that their roles as directors, guiders are important. The majoring students don’t have enough practicing places and factories. Even though they are received training about job in school, they get jobs unrelated with their received training. And they have verγ low employment as a whole. Though the transitional education program whose aims are selection in jobs, employment and enjoying jobs, it is started especially in the middle and high school courses. And it is thought that official and individualized education program is very important, also needed the cooperation among related authorities and it’s major program is used as the programs on job training. programs on job training. Next, as to the problems and improved methods, the problems about the majored subjects are the unrelatedness with job training and unreality and only one line of selection in job training. The problem about the transitional education program is the shortage in social skills, in the basement of job training and in carrγing out ability. The professional ability which is required in job guiding teacher is job training guide, many methods, the ability about assessing job aptitude, and testing their ability. The construction of the curriculum is not applying the guided curriculum rather than transforming the curriculum according to the atmosphere, and their school year should not be stuck to any one standard but be varied according to their ability and improvement. In the operation of majored subjects, it is necessarγ for them to provide real career education, the opportunity to receive various job training and the supported employments in which they can be included in their society actively.

신원방우황청심원액의 심혈관계에 관한 약효

조태순,이선미,김낙두,허인회,안형수,권광일,박석기,심상호,신대희,박대규 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-In order to investigate the pharmacological properties of New Wonbang Woohwangchungsimwon Liquid (NSCL), effects of Wonbang Woohwangchungsimwon Liquid (SCL) and NSCL were compared. In isolated rat aorta, NSCL and SCL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10^-6 M) regardless to intact endothelium or denuded rings of the rat aorta. Furthermore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxing effect of NSCL and SCL. NSCL and SCL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs), NSCL and SCL significantly decreased heart rate. NSCL and SCL, at high doses, had a negative inotropic effect that was a decrease of left ventricular developed pressure and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NSCL and SCL had no effects on parameters of action potential such as resting membrane potential, action potential amplitude, APD_90 and V_max at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NSCL and SCL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between cardiovascular effects of two preparations.