Dynamic Patterns of PTK7 Protein Expression in Adult Mouse Tissues

( Jihee Kim ),( Ho Yoon ),( Song Ee Lee ),( Won Suk Kang ),( Ik Soo Jeon ),( Da Jeong Chang ),( Nayeon Lee ),( Taesun Hwang ),( Won Sik Shin ),( Han Woong Lee ),( Seung Taek Lee ),( Ji Hwan Song ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

PTK7 belongs to a subgroup of receptor protein tyrosine kinases with inactive catalytic activity of the protein tyrosine kinase(PTK), which is known to be important for signal attenuation, cell adhesion, and regulation of planar cell polarity. In this study, to decipher possible biological roles of PTK7, we examined the tissue distribution and localization of PTK7 protein in adult mouse tissues. PTK7-positive signals were detected in variety of tissues and cells in multiple systems, including respiratory, digestive, urinary and reproductive organs, as well as liver and pancreas. These expression profiling data imply various physiological roles of PTK7 in multiple organ systems. Interestingly, among various tissues examined, no specific signal was detected in the heart and muscles. Taken together, dynamic patterns of protein expression in adult tissues strongly suggest PTK7 may play important roles during organogenesis and histogenesis.

Immobilization of Copper Hexacyanoferrate on Highly Porous Al2O3 Ceramic Foam for Remediation of Radioactive Cs Contaminated Water

Jihee Song,Chaerin Park,Hye-Jin Hong 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.2

Copper hexacyanoferrate (Cu-HCF), which is a type of Prussian Blue analogue (PBA), possesses a specific lattice structure that allows it to selectively and effectively adsorb cesium with a high capacity. However, its powdery form presents difficulties in terms of recovery when introduced into aqueous environments, and its dispersion in water has the potential to impede sunlight penetration, possibly affecting aquatic ecosystems. To address this, sponge-type aluminum oxide, referred to as alumina foam (AF), was employed as a supporting material. The synthesis was achieved through a dip-coating method, involving the coating of aluminum oxide foam with copper oxide, followed by a reaction with potassium hexacyanoferrate (KHCF), resulting in the in-situ formation of Cu-HCF. Notably, Copper oxide remained chemically stable, which led to the application of 1, 3, 5-benzenetricarboxylic acid (H3BTC) to facilitate its conversion into Cu-HCF. This was necessary to ensure the proper transformation of copper oxide into Cu-HCF on the AF in the presence of KHCF. The synthesis of Cu-HCF from copper oxide using H3BTC was verified through X-ray diffraction (XRD) analysis. The manufactured adsorbent material, referred to as AF@CuHCF, was characterized using Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). These analyses revealed the presence of the characteristic C≡N bond at 2,100 cm-1, confirming the existence of Cu-HCF within the AF@CuHCF, accounting for approximately 3.24% of its composition. AF@CuHCF exhibited a maximum adsorption capacity of 34.74 mg/g and demonstrated selective cesium adsorption even in the presence of competing ions such as Na+, K+, Mg2+, and Ca2+. Consequently, AF@CuHCF effectively validated its capabilities to selectively and efficiently adsorb cesium from Cs-contaminating wastewater.

Surgery and Metronomic Chemotherapy in a Pet Rabbit (Oryctolagus cuniculus) with Mammary Gland Adenocarcinoma

Jihee Hong,Jeong-Min Lee,Ji-Young Lee,Han-Joon Lee,Dong-Kwan Lee,Joong-Hyun Song,Kun-Ho Song The Korean Society of Veterinary Clinics 2023 한국임상수의학회지 Vol.40 No.6

An 8-year-old female pet rabbit presented at the veterinary clinic for mammary gland palpation due to the presence of a mass. Upon physical examination, a mass was identified in the left fourth mammary gland. Abdominal ultrasonography revealed a 3 × 2 cm mass in the right uterus and general thickening of the endometrium, suggesting uterine sinusitis. Multiple pulmonary nodules suspected to be metastatic lesions were identified on chest radiography. Surgery was performed to mastectomy and ovariohysterectomy (OHE). The histopathological examination of the tumor revealed mammary gland adenocarcinoma (simple-type) with multiple nodules consisting of the proliferation of tumor cells forming tubules containing secretory materials, cellular debris, and solid nests with a central area of necrosis. Metronomic chemotherapy was performed with cyclophosphamide and lomustine (CCNU) based on the histopathological findings. The quality of life has been well maintained, with no specific clinical symptoms observed for 8 months after metronomic chemotherapy. To the best of authors' knowledge, this study is the first to examine the effects of metronomic chemotherapy with cyclophosphamide and lomustine in a pet rabbit.

Butyric Acid Production using Glucose and Brown Algae using Clostridium tyrobutyricum ATCC 25755

Jihee SONG,Deokjin JAHNG 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10

Molecular Phyogeny of Anthozoans (Phylum Cnidaria)Based on the Nucleotide Sequences of 18S rRNA gene

Song, Jun-Im,Kim, Won,Kim, Eun Kyoung,Kim, Jihee 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

산호충류의 분자계통을 밝히기 위하여 자포동물에 속하는 6종에 대한 18 rRNA 유전자의 염기서열을 비교하였다. 본 연구에 사용된 종들은 산호충 강에 속하는 5종(팔방산호충 아강의 Bellonella rigida, 육방산호충 아강의 Anemonia sulcata, Anthopleura kurogane, Anthopleura midori 및 꽃말미잘 아강의 Cerianthus filiformis )과 히드로충 강에 속하는 1종 Cornyne pusilla 이었다. 정열된 염기서열은 Distance matrix method와 parsimony method를 사용하여 기존의 산호충류 계통을 검증해 보았다. 그 결과 산호충류는 단일계통군이었고, 신호충 강을 3아강으로 나누는 기존의 체계는 지지되었지만, 꽃말미잘 아강은 다른 아강보다 더 원시적인군인 것으로 나타났다. 또한, 해변말미잘 과는 산호충 강내의 단일계통군이지만, 속간의 계통유연관계는 명확하지 않았다. The partial nucleotide sequences of 18S ribosomal RNA gene were compared for six cnidarian species (one belongs to class Hydrozoa: Coryne pusilla. Five belong to clas Anthozoa: Bellonella rigida from Octocorallia; Anemonia sulcata, Anthopleura kurogane, Anthopleura midori from Hexacorallia; Cerianthus filiformis from Ceriantipatharia). The aligned sequence data were used to test the hypothesis on the anthozoan phylogeny by using the distance matrix method and parsimony method. The phylogenetic inferences resulting from these methods indicate that the anthozoan is a monophyletic group and support the three subclass scheme (Octocorallia, Hexacorallia, Ceriantipatharia) within class Anthozoa. The result also indicates that ceriantipatharian is more primitive than the other groups and that family Actiniidae is a monophyletic group within the anthozoan. However, the present analysis does not clearly indicate the phylogenetic relationships of species among genera.

Assessment of radiographic left atrial dimension and C-reactive protein in dogs with myxomatous mitral valve disease

Jihee Hong,Han-Joon Lee,Dong-Kwan Lee,Kun-Ho Song 한국동물위생학회 2024 韓國家畜衛生學會誌 Vol.47 No.1

Radiographic left atrial dimension (RLAD) is a valuable metric for assessing left atrial enlargement in dogs. While there have been studies on the use of RLAD and the increase in C-reactive protein (CRP) levels based on heart disease stages, there has been no prior research on the correlation between RLAD and CRP. In this study, the objective was to investigate the relationship between the rise in RLAD as myxomatous mitral valve disease (MMVD) stages advance and the increase in CRP levels with MMVD stage progression. In this study, a total of 30 small-breed dogs were included as subjects. These dogs were diagnosed with MMVD at the American College of Veterinary Internal Medicine (ACVIM) stage B1 or B2, or stage C, based on a comprehensive assessment including physical examination, thoracic radiography, and echocardiography. Measurements of VHS and RLAD were compared to assess any significant differences. There were significant differences in RLAD between dogs with MMVD ACVIM stage B1 and those with stage C. The monocytes and CRP levels showed significant differences between ACVIM stage B1, B2 and ACVIM C. Additionally, a significant correlation was observed between the RLAD and VHS measurements. This underscores the notable association between MMVD stage advancement and elevated monocyte and CRP levels. The RLAD scores exhibited a significant difference among dogs with ACVIM stages B1, B2, and C, and significant variations were also observed in monocyte and CRP levels. These results suggest that monocyte and CRP levels may be a valuable diagnostic indicator for heart disease in dogs during the diagnostic evaluation.

IT 센싱 기술을 이용한 전통주 발효의 품질관리 연구

송혜지(Hyeji Song),최지희(Jihee Choi),박찬원(Chan-Won Park),신동범(Dong-Beom Shin),강성수(Sung-Soo Kang),오성훈(Sung Hoon Oh),황권택(Kwontack Hwang) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.6

전통주는 성장할 수 있는 전기를 맞이하고 있으나 전통적인 생산방식으로 인한 표준화, 품질관리의 문제점을 안고 있다. 이에 sensor 기술과 RFID 기술을 이용하여 품질관리와 표준화문제에 접근하고자 하였다. RFID 기술은 원거리 Sensing과 즉각적인 제어로서 표준화와 품질의 고급화를 이룰 수 있는 최적화 기술이다. 본 실험에서 3차에 걸친 발효과정을 측정하였는데 발효조의 내부온도에 따라 발효종말점이 14, 17, 20일까지 달랐다. pH의 경우 발효 초기 pH가 7.89, 7.95, 7.68에서 최종 pH 3.31에서 2.96까지 급격한 pH의 강하가 이뤄졌고 이후 서서히 상승으로 진행하다 최종에 pH 3.34에 도달하였다. 총산의 경우 발효 초기 0.1, 0.2, 0.1%에서 3~4일까지 급격히 총산이 증가하였고 이후 완만한 상승이 이어지다 최종에 pH 2.3∼2까지 낮아졌다. 이는 발효기간 중에 생성된 유기산과 밀접한 관계가 있다. 당도의 측정은 발효기에 부착한 sensor를 통하여 자료를 얻었는데 발효에서 당도의 변화는 초기 급격한 상승을 이룬 다음 중간발효에서는 점차 낮아졌고 발효 후기에 갈수록 점차 증가하는 경향을 보였다. 알코올의 측정과 총당의 측정은 아직까지 자동으로 검출이 어려워 manual 방식으로 측정하였는데, 알코올 함량은 초기에 급격히 증가하다가 발효 5일째서부터 대부분 완만한 증가를 보였고 1차 발효에서는 17.3%, 2차발효에서는 16.7%, 3차 발효에서는 17.1%에서 발효가 완료되었다. 유리당은 1일째 4,171.44 mg%를 나타내다 초기에 급격한 감소를 보였고 10일을 전후하여 증가폭의 변화가 둔화되었다. 전체 중에 glucose가 제일 많은 함량이었고, 다음으로 sucrose, fructose 순이었다. 유기산의 경우 초기에 전체 61.48 mg%를 보이면서 발효 11일째까지 88.09mg%를 보여 아주 완만한 상승이었는데, 이후 급격한 상승을 보였고 14일째 266.21 mg%에서 정점을 찍고 이후 완만한 진행을 보였다. 전통주의 발효에서 품질관리를 통한 전통주의 고급화 방법의 하나로 RFID 기술을 도입하여 이상발효의 차단과 적정한 sensor를 이용하여 지표로 활용, 정확한 발효과정을 판단할 필요가 있다. pH, 총산, 알코올 그리고 총당은 전통주 품질관리의 지표로 활용 가능한 바, 전통주 발효 전 과정을 monitering 하여 이상발효 유무의 중요한 지표로 활용이 가능할 것이다. The objective of this study was to investigate the quality characteristics of traditional wine using an radio-frequency identification (RFID) system annexed to a fermenter. In this study, we proposed an RFID-based data transmission scheme for monitoring fermentation of traditional alcoholic beverages. The pH, total acidity, total sugar, soluble sugar, free sugar, alcohol content, and organic acids of were investigated and subjected to fermentation of traditional alcoholic beverages three times. The pH ranged from 7.98, 7.95, and 7.68 at day 0, decreased drastically to 3.31∼2.96 at day 2, and then slowly increased to the end point, finally reaching 3.34 at day 20. Acidity tended to increase quickly with time, especially for all samples after day 2. The fermentation environment induced a sudden increase acidity in reactants and indicated a low pH. The total sugars during fermentation quickly decreased to the range of 20.3, 22.43, and 19.2% at day 2, and the slope of reduction steadily decreased to 5.1, 6.1, and 4.8% at day 10. On the other hand, the alcohol content showed the reverse trend as total sugars. The alcohol content also showed the same pattern as total acids, showing the highest alcohol content of 17.3% (v/v) on day 20. In this study on traditional wine fermentation using an RFID system, we showed that pH, soluble sugar, and alcohol content can be adopted as key indicators for quality control and standardization of traditional wine manufacturing.

배전DAS-업무망 보안 이슈 및 물리적 일방향 자료전달 시스템 적용 방안

김지희(KIM Jihee),김진철(KIM Jincheol),박성완(PARK Sungwan),송주영(Song Jooyoung) 한국정보보호학회 2014 情報保護學會誌 Vol.24 No.5

제어시스템은 일반 업무환경에서 사용하고 있는 시스템과는 달리 업무망과 분리되어 운영되며, 비공개 제어프로토콜 사용으로 해커나 악성코드에 의해 사이버공격을 받을 가능성이 없다고 인식되어 왔다. 그러나 2010년 스턱스넷(Stuxnet) 악성코드가 이란의 핵시설 제어시스템에 침투하였고 2012년 듀크, 플레임 등 제어시스템을 대상으로 한 위협이 증가하게 되면서 제어시스템과 업무망 연계 지점의 보안 위협을 제거하기 위한 방안으로 물리적 일방향 자료전달 시스템의 도입이 추진되어 왔다. 본 논문에서는 배전DAS 업무망 연계를 위한 물리적 일방향 자료전달 시스템 구현에 대해서 설명하고 실증에 따른 결과를 제시한다.

Characteristic of back fat and quality of longissimus dorsi muscle from soft fat pork carcasses

Daewoon Lim,Minho Song,Juri Lee,Chulwoo Lee,Jaechung Lee,Wangyeol Lee,Jihee Seo,Samooel Jung 충남대학교 농업과학연구소 2016 Korean Journal of Agricultural Science Vol.43 No.4

물리적 망분리 자료연계시스템 개발 및 실증

박성완(Park SungWan),김지희(Kim JiHee),김진철(Kim JinChul),송주영(Song JooYuong),이승원 대한전기학회 2015 대한전기학회 학술대회 논문집 Vol.2015 No.5

물리적 일방향 자료전달 기술은 망간 자료전송 기술 중 하나로, 외부망에서 업무망으로의 물리적 데이터 전송회선을 제거하여 침입경로를 완전히 차단한다. 국정원에서는 전자제어시스템 보안 가이드라인 지침을 만들어 국가 기반시설의 보안을 강화하고 있다. 이에 따라 한국전력공사는 전력 제어망에 대한 보안 물리적 일방향 자료전달 장치(1)을 구축하고 운영하고 있다. 초기 시스템 도입부터 운영하기까지 물리적 망분리 네트워크 상황에서의 자료연계 시스템개발과 실증시험 결과를 소개하고자 한다 .