Epigallocatechin-3-gallate suppresses hemin-aggravated colon carcinogenesis through Nrf2-inhibited mitochondrial reactive oxygen species accumulation

석주형,Dae Hyun Kim,Hye Jih Kim,Hang Hyo Jo,김은영,Jae-Hwang Jeong,Young Seok Park,Sang Hun Lee,Dae Joong Kim,Sang Yoon Nam,Beom Jun Lee,Hyun Jik Lee 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.5

Background: Previous studies have presented evidence to support the significant association between red meat intake and colon cancer, suggesting that heme iron plays a key role in colon carcinogenesis. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, exhibits anti-oxidative and anti-cancer effects. However, the effect of EGCG on red meat-associated colon carcinogenesis is not well understood. Objectives: We aimed to investigate the regulatory effects of hemin and EGCG on colon carcinogenesis and the underlying mechanism of action. Methods: Hemin and EGCG were treated in Caco2 cells to perform the water-soluble tetrazolium salt-1 assay, lactate dehydrogenase release assay, reactive oxygen species (ROS) detection assay, real-time quantitative polymerase chain reaction and western blot. We investigated the regulatory effects of hemin and EGCG on an azoxymethane (AOM) and dextran sodium sulfate (DSS)-induced colon carcinogenesis mouse model. Results: In Caco2 cells, hemin increased cell proliferation and the expression of cell cycle regulatory proteins, and ROS levels. EGCG suppressed hemin-induced cell proliferation and cell cycle regulatory protein expression as well as mitochondrial ROS accumulation. Hemin increased nuclear factor erythroid-2-related factor 2 (Nrf2) expression, but decreased Keap1 expression. EGCG enhanced hemin-induced Nrf2 and antioxidant gene expression. Nrf2 inhibitor reversed EGCG reduced cell proliferation and cell cycle regulatory protein expression. In AOM/DSS mice, hemin treatment induced hyperplastic changes in colon tissues, inhibited by EGCG supplementation. EGCG reduced the hemin-induced numbers of total aberrant crypts and malondialdehyde concentration in the AOM/DSS model. Conclusions: We demonstrated that EGCG reduced hemin-induced proliferation and colon carcinogenesis through Nrf2-inhibited mitochondrial ROS accumulation.

HCC : PE-062 ; The Radiotherapy on HCC in caudate lobe

( Hyun Jung Kim ),( Jih Ye Cha ),( Hae Ok Pyun ),( Jin Sil Seong ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: Hepatocelluar carcinoma (HCC) in caudate lobe has poor prognosis and is challenging to treat with surgery, radiofreqeuncy ablation (RFA), transcatheter arterial embolization (TACE) because of its unique anatomic location. This study was designed to assess the efficacy and safety of radiotherapy (RT) for HCC in the caudate lobe. Methods: Between January 1996 and August 2011, 58 patients with HCC in caudate lobe were treated with RT. Fifty cases were treated after the failure of previous RFA or TACE. Eight patients were treated as definitive aim. Most of the patients were treated with RT alone (44%) while the others were treated with RT with intra-arterial (34%) or systemic chemotherapy (15%), RT after TACE (7%) within 1 month. Median radiation dose was 45Gy (range, 30.6 - 80 Gy) in 25 fractions. The tumor response was determined by CT scans at post-RT 3 months. Results: The median follow-up period after RT was 10.6 months (range, 1 ? 93 months). The 1 year- and 2 year-local control rates were 73.5% and 47.7%, respectively, and 1 yearand 2 year-overall survival rates were 53.8% and 40.4%, respectively. Complete response was achieved in two patients. In-field local control was achieved in 70% of patients. During the follow-up, 14 patients (24%) developed intra-hepatic metastases and eight patients (13%) developed extra-hepatic metastases: lung, distant lymph nodes, bone, and brain. Seven patients developed grade 1 nausea or anorexia during RT. There were no grade 3 or greater treatment-related toxicities. Conclusions: Radiotherapy appears to be an effective and feasible treatment modality for HCC in caudate lobe.

HCC : PE-063 ; The therapeutic efficacy of radiotherapy for HCC in caudate lobe

( Hyun Jung Kim ),( Jih Ye Cha ),( Hae Ok Pyun ),( Jin Sil Seong ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background: Hepatocellular carcinoma (HCC) in caudate lobe has poor prognosis due to unsatisfactory effect with currenttreatment including surgery, radiofrequency ablation (RFA), transcatheter arterial embolization (TACE) because of its unique anatomic location. This study was designed to assess the efficacy and safety of radiotherapy (RT) for HCC in the caudate lobe. Methods: Between January 1996 and August 2011, 58 patients with HCC in caudate lobe were treated with RT. Fifty patients were treated after the failure of previous RFA or TACE and 8 patients, as definitive aim. Most of the patients were treated with RT alone (44%) while the others were treated with RT with intra-arterial (34%) or systemic chemotherapy (15%), RT after TACE (7%) within 1 month. Median radiation dose was 45Gy (range, 30.6 - 80 Gy) in 25 fractions. The tumor response was determined by CT scans at post-RT 3 months. Results: The median follow-up period after RT was 10.6 months (range, 1?93 months). The 1- and 2- year local control rates were 73.5% and 47.7%, respectively, and 1- and 2- year overall survival rates were 53.8% and 40.4%, respectively. Complete response was achieved in two patients. In-field local control was achieved in 70% of patients. During the follow-up, 14 patients (24%) developed intra-hepatic metastases and eight patients (13%) developed extra-hepatic metastases: lung, distant lymph nodes, bone, and brain. Seven patients developed grade 1 nausea or anorexia during RT. There were no grade 3 or above treatment-related toxicities. Conclusions: Radiotherapy appears to be an effective and feasible treatment modality for HCC in caudate lobe.

Influence of the gas mixture ratio on the electrical and ferroelectric properties of PLZT thin films

Sang-Jih Kim,손영국,Dong-Hyun Hwang,In-Seok Lee,Jung-Hoon Ahn 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.4

A (Pb0.92La0.08)(Zr0.65Ti0.35)O3 (PLZT) ferroelectric thin film was deposited on deposited on TiO2 buffered layer on top of a Pt/Ti/SiO2/Si substrate by RF magnetron sputtering method. The effect of the Ar/O2 partial pressure ratio on the ferroelectric properties of PLZT thin films was investigated at various Ar/O2 partial pressure ratios such as 27/1.5, 23/5.5, 21/7.5 and 19/9.5 sccm. The crystallinities of PLZT thin films were analyzed by XRD. The surface morphology was characterized by FESEM. The P-E hysteresis loops, the remnant polarization characteristics and the leakage current characteristics were obtained using a precision LC. With an increase of the oxygen partial pressure ratio, the crystallinity was decreased. Also the preferred orientation of PLZT thin films was changed from the (110) plane to the (111) plane. Results indicated that the change of the oxygen partial pressure ratio significantly affects the thin film surface morphology and the ferroelectric properties.

RF Magnetron Sputtering법으로 제작된 (Pb<SUB>0.92</SUB>La<SUB>0.08</SUB>)(Zr<SUB>0.65</SUB>Ti<SUB>0.35</SUB>)O₃ 박막의 Ar/O₂ 분압비에 따른 강유전 특성연구

김상지(Sang-Jih Kim),윤지언(Ji-Eon Yoon),황동현(Dong-Hyun Hwang),이인석(In-Seok Lee),안정훈(Jung-Hoon Ahn),손영국(Young-Guk Son) 한국진공학회(ASCT) 2009 Applied Science and Convergence Technology Vol.18 No.2

rf magnetron sputtering 법을 이용하여 Pt/Ti/SiO₂/Si 기판 위에 buffer layer인 TiO₂ 층을 증착한 후 PLZT 강유전체 박막을 증착하였다. PLZT 박막 증착 시 가스 분압비가 박막의 특성에 미치는 영향을 알아보기 위해 Ar/O₂ 분압비를 각각 27/1.5 sccm, 23/5.5 sccm, 21/7.5 sccm, 19/9.5 sccm로 변화시키면서 박막을 증착하였다. 이들 박막의 구조적인 특성을 분석하기 위해 X-선 회절법을 사용하였으며 FE-SEM을 이용하여 입자상을 관찰하였다. 또한 박막의 유전특성을 분석하기 위해 Precision LC를 이용하여 이력곡선, 잔류분극, 누설전류를 측정하였다. 산소 분압이 높아질수록 박막의 결정성 및 치밀성이 저하되었으며, (110) 방향에서 (111) 방향으로 우선배향성이 변화하는 것을 확인하였다. 산소 분압비의 변화는 박막 표면 및 강유전 특성에 영향을 미치는 것으로 판단된다. PLZT ferroelectric thin films were deposited on Pt/Ti/SiO₂/Si substrate with TiO₂ buffer layer in between by rf magnetron sputtering method. In order to investigate the effect of Ar/O₂ partial pressure ratio on the ferroelectric properties of PLZT thin films, PLZT thin films were deposited at various Ar/O₂ partial pressure ratio ; 27/1.5 sccm, 23/5.5 sccm, 21/7.5 sccm and 19/9.5 sccm. The crystallinities of PLZT thin films were analyzed by XRD. The surface morphology was observed using FE-SEM. The P-E hysteresis loops, the remanent polarization characteristics and the leakage current characteristics were obtained using a Precision LC. The crystallinity and elaborateness of PLZT thin films were decreased as increasing the oxygen partial pressure ratio. And preferred orientation of PLZT thin films changed from (110) plane to (111) plane. The oxygen partial pressure ratio affects the thin film surface morphology and the ferroelectric properties.

Cooperation and Functional Diversification of Two Closely Related Galactolipase Genes for Jasmonate Biosynthesis

You Bong Hyun,Sung Wool Choi,Hyun Ju Hwang,Jih Yeon Yu,Sang Jip Nam,Jaeyoung Ko,Ju Young Park,Young Sam Seo,Eun Yu Kim 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.39 No.-

Plenary Session 1 : PS-1-5 ; Is HBsAg seroclearance following nucleoside analogue therapy durable in patients with chronic hepatitis B?

( Gi Ae Kim ),( Young Suk Lim ),( Ju Hyun Shim ),( Kang Mo Kim ),( Han Chu Lee ),( Yung Sang Lee ),( Young Hwa Chung ),( Dan Bi Lee ),( Jih Yun An ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Spontaneous or interferon-induced HBsAg seroclearance is durable in most patients with chronic hepatitis B (CHB). However, little is known about the durability of HBsAg seroclearance following nucleoside analogue (NUC) therapy. Methods: Among 4,578 patients who were treated with either lamivudine (n=1,924) or entecavir (n=2,654) at a tertiary referral hospital in Korea between 2000 and 2010, 121 achieved HBsAg seroclearance. Fifty-eight patients were included in this study after exclusion of 63 patients; acute hepatitis B (n=19), hepatocellular carcinoma (n=8), prior treatment with interferon (n=6), prior immunosuppressive therapy (n=17), liver transplantation (n=6), follow-up loss immediately after HBsAg seroclearance (n=5), and continued NUC therapy (n=2). Results: Mean age of 58 patients were 42 (SD 11) years and 41 (71%) were males. All were assumed to have HBV genotype C. At the initiation of NUC therapy, median levels of ALT and HBV DNA were 153 IU/L (interquartile range [IQR], 48-340 IU/L) and 7.0 log10 copies/mL (IQR, 3.8-8.0 log10 copies/mL), respectively. Twenty-seven (47%) had HBeAg. The median duration of NUC therapy (56 with lamivudine and 2 with entecavir) before HBsAg seroclearance was 42 months (IQR, 24-66 months). During a median follow-up period of 20 months (IQR, 12-32 months), HBsAg reversion occurred in 5 of 58 (8.6%) patients. Three of those 5 patients achieved re-clearance of HBsAg without treatment during further follow-up. The other 2 patients remained HBsAg-positive, but with low titer (<1.0 IU/mL) and undetectable HBV DNA by PCR. Virologic recurrence (detectable HBV DNA by PCR) occurred in 12 of 58 (20.7%) patients. However, all of these patients maintained HBV DNA <10,000 copies/mL. No patient experienced biochemical relapse (ALT flare > x5 ULN). Conclusion: HBsAg seroclearance following NUC therapy is rare but durable in most patients with CHB after treatment discontinuation. Therefore, HBsAg seroclearance would be an ideal treatment endpoint during NUC therapy.

Suppressive effects of pectin on colitis-associated colon carcinogenesis

Young Jae Yoo,Hye Jih Kim,Dae Hyun Kim,Young Seok Park,Sang Yoon Nam,Beom Jun Lee,Hyun Jik Lee 충북대학교 동물의학연구소 2021 Journal of Biomedical and Translational Research Vol.22 No.4

RF Magnetron Sputtering법으로 제작된 (Pb_0.92La_0.08)(Zr_0.65Ti_0.35)O₃ 박막의 Ar/O₂ 분압비에 따른 강유전 특성연구

김상지,윤지언,황동현,이인석,안정훈,손영국,Kim, Sang-Jih,Yoon, Ji-Eon,Hwang, Dong-Hyun,Lee, In-Seok,Ahn, Jung-Hoon,Son, Young-Guk 한국진공학회 2009 Applied Science and Convergence Technology Vol.18 No.2

rf magnetron sputtering 법을 이용하여 Pt/Ti/$SiO_2$/Si 기판 위에 buffer layer인 $TiO_2$ 층을 증착한 후 PLZT 강유전체 박막을 증착하였다. PLZT 박막 증착 시 가스 분압비가 박막의 특성에 미치는 영향을 알아보기 위해 Ar/$O_2$ 분압비를 각각 27/1.5 sccm, 23/5.5 sccm, 21/7.5 sccm, 19/9.5 sccm로 변화시키면서 박막을 증착하였다. 이들 박막의 구조적인 특성을 분석하기 위해 X-선 회절법을 사용하였으며 FE-SEM을 이용하여 입자상을 관찰하였다. 또한 박막의 유전특성을 분석하기 위해 Precision LC를 이용하여 이력곡선, 잔류분극, 누설전류를 측정하였다. 산소 분압이 높아질수록 박막의 결정성 및 치밀성이 저하되었으며, (110) 방향에서 (111) 방향으로 우선배향성이 변화하는 것을 확인하였다. 산소 분압비의 변화는 박막 표면 및 강유전 특성에 영향을 미치는 것으로 판단된다. PLZT ferroelectric thin films were deposited on Pt/Ti/$SiO_2$/Si substrate with $TiO_2$ buffer layer in between by rf magnetron sputtering method. In order to investigate the effect of Ar/$O_2$ partial pressure ratio on the ferroelectric properties of PLZT thin films, PLZT thin films were deposited at various Ar/$O_2$ partial pressure ratio ; 27/1.5 seem, 23/5.5 seem, 21/7.5 seem and 19/9.5 seem. The crystallinities of PLZT thin films were analyzed by XRD. The surface morphology was observed using FE-SEM. The P-E hysteresis loops, the remanent polarization characteristics and the leakage current characteristics were obtained using a Precision LC. The crystallinity and elaborateness of PLZT thin films were decreased as increasing the oxygen partial pressure ratio. And preferred orientation of PLZT thin films changed from (110) plane to (111) plane. The oxygen partial pressure ratio affects the thin film surface morphology and the ferroelectric properties.

The ferroelectric and electrical properties of PLZT thin films

In-Seok Lee,Ji-Eon Yoon,Dong-Hyun Hwang,Sang-Jih Kim,Jung-Hoon Ahn,손영국 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.4

La-doped lead zirconate titanate (PLZT) thin films were fabricated on Pt/Ti/SiO2/Si substrates by RF magnetron sputtering method. The crystallinity and the electrical properties of the films were investigated as a function of deposition temperature. As the deposition temperature was increased, the preferred orientation of films was changed from the (110) to the (111) plane. The (110) plane decreases with further increase of the substrate temperature to 500℃. The PLZT films deposited to 400℃ showed good electric properties with a remnant polarization of 15.8 μC/㎠ and a leakage current of 5.4 × 10-9 A/㎠. As the deposition temperature was increased 500℃, the PLZT films tended to be Pb-deficient, resulting in the degradation of electrical properties.