Notch Signaling Pathway: Rational Targets for Cancer Therapy

Jieun Yun, Sang-Bae Han, Youngsoo Kim 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.2

본문참고

구체물을 활용한 음악표상활동이 유아의 음악적 능력 및 표상능력에 미치는 영향

배지은 ( Bae Jieun ),백지혜 ( Baek Jeehea ) 미래유아교육학회 2018 미래유아교육학회지 Vol.25 No.2

본 연구의 목적은 구체물을 활용한 음악표상활동이 만 5세 유아의 음악적 능력 및 표상능력에 미치는 영향에 대해 알아보는 것이다. 연구 대상은 D광역시에 위치한 유치원 만 5세반 유아 51명으로 한 학급 26명을 실험집단으로, 다른 학급 25명을 통제집단으로 선정하였다. 실험집단은 구체물을 활용한 음악표상활동을 10주간 주 2회 총 20회에 걸쳐 실행하였으며, 통제집단은 누리과정에 기초한 음악활동을 실행하였다. 연구에 사용된 도구는 음악적 능력 측정을 위해 Gordon(1986)이 개발한 PMMA(The Primary Measures of Music Audiation)를 사용하였으며, 표상능력 측정을 위해 신은수·권미경(2011)의 점토활동을 위한 표상능력 평가도구를 사용하였다. 본 연구에서 수집된 자료는 공분산분석(ANCOVA)을 실시하여 분석하였다. 본 연구의 결과는 다음과 같다. 첫째, 구체물을 활용한 음악표상활동은 음악적 능력 전체 및 하위요인인 음감 능력, 리듬감 능력의 향상에 통계적으로 유의미한 효과를 나타내었다. 둘째, 구체물을 활용한 음악표상활동은 표상능력 전체 및 하위요인인 표상적 주제 관련성, 표상적 정교성, 표상적 창의성, 표상적 언어 연계성 향상에 통계적으로 유의미한 효과를 나타내었다. 즉, 구체물을 활용한 음악표상활동은 유아의 음악적 능력 및 표상능력을 증진시키는데 효과적이라고 할 수 있다. The purpose of this study was to investigate the effects of musical representation activities utilizing concrete materials on young children’s musical and representational ability. This study recruited fifty-one children of five-year-old classrooms from a kindergarten located in Daegu metropolitan city. They were subdivided into an experimental(n = 26) and a control group(n = 25). The experimental group participated in the musical representation activities using concrete materials for a total of twenty sessions over ten weeks, two times per week, while the control group experienced musical activities based on the Nuri curriculum. The tools used in this study were the Primary Measures of Music Audiation (PMMA) by Gordon(1986) in order to measure their musical abilities and the Representational Evaluation Scale for Clay Activities by Shin and Kwon(2011) in order to measure their representational abilities. The collected data were analyzed using ANCOVA to explore the differences of musical and representational ability between the two groups. The results of this study were as follows: First, the children's musical ability and its sub-categories, which were tonal and rhythm capabilities in the experimental group that participated in the activities using concrete materials significantly differed from those of the control group. Second, the children’s representational ability and its sub-categories, which were elaboration of representation, creativity of representation, connectedness of representation of language in the experimental group were significantly different from those of the control group. The findings suggest that musical representational activities based on concrete materials are productive in achievement of young children’s musical and representational abilities.

Exosomal miR-181b-5p Downregulation in Ascites Serves as a Potential Diagnostic Biomarker for Gastric Cancer-associated Malignant Ascites

Yun, Jieun,Han, Sang-Bae,Kim, Hong Jun,Go, Se-il,Lee, Won Sup,Bae, Woo Kyun,Cho, Sang-Hee,Song, Eun-Kee,Lee, Ok-Jun,Kim, Hee Kyung,Yang, Yaewon,Kwon, Jihyun,Chae, Hee Bok,Lee, Ki Hyeong,Han, Hye Sook The Korean Gastric Cancer Association 2019 Journal of gastric cancer Vol.19 No.3

Purpose: Peritoneal carcinomatosis in gastric cancer (GC) patients results in extremely poor prognosis. Malignant ascites samples are the most appropriate biological material to use to evaluate biomarkers for peritoneal carcinomatosis. This study identified exosomal MicroRNAs (miRNAs) differently expressed between benign liver cirrhosis-associated ascites (LC-ascites) and malignant gastric cancer-associated ascites (GC-ascites), and validated their role as diagnostic biomarkers for GC-ascites. Materials and Methods: Total RNA was extracted from exosomes isolated from 165 ascites samples (73 LC-ascites and 92 GC-ascites). Initially, microarrays were used to screen the expression levels of 2,006 miRNAs in the discovery cohort (n=22). Subsequently, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analyses were performed to validate the expression levels of selected exosomal miRNAs in the training (n=70) and validation (n=73) cohorts. Furthermore, carcinoembryonic antigen (CEA) levels were determined in ascites samples. Results: The miR-574-3p, miR-181b-5p, miR-4481, and miR-181d were significantly downregulated in the GC-ascites samples compared to the LC-ascites samples, and miR-181b-5p showed the best diagnostic performance for GC-ascites (area under the curve [AUC]=0.798 and 0.846 for the training and validation cohorts, respectively). The diagnostic performance of CEA for GC-ascites was improved by the combined analysis of miR-181b-5p and CEA (AUC=0.981 and 0.946 for the training and validation cohorts, respectively). Conclusions: We identified exosomal miRNAs capable of distinguishing between non-malignant and GC-ascites, showing that the combined use of miR-181b-5p and CEA could improve diagnosis.

Extraction of Separated Source and Drain Resistances in Amorphous Indium–Gallium–Zinc Oxide TFTs Through <tex> $C$</tex>– <tex> $V$</tex> Characterization

Hagyoul Bae,Sungchul Kim,Minkyung Bae,Ja Sun Shin,Dongsik Kong,Hyunkwang Jung,Jaeman Jang,Jieun Lee,Dae Hwan Kim,Dong Myong Kim IEEE 2011 IEEE electron device letters Vol.32 No.6

<P>Considering asymmetry caused by layout, process, and device degradation, separate extraction of the source and drain resistances, i.e., <I>RS</I> and <I>RD</I>, respectively, from the total resistance <I>R</I><SUB>TOT</SUB> is very important in the design, modeling, and characterization of amorphous indium-gallium-zinc oxide (a-IGZO) thin-film transistors (TFTs). Due to the insulated gate structure, however, separate extraction is difficult through direct-current <I>I</I>-<I>V</I> characterization. In this letter, we propose a simple and useful technique for separate extraction of <I>RS</I> from <I>RD</I> in a-IGZO TFTs through a two-terminal parallel-mode <I>C</I> -<I>V</I> technique. We experimentally verified the validity of the proposed technique by comparing the result with the source-to-drain resistance from the <I>I</I>-<I>V</I> characteristics.</P>

잔류유분 함유 반출처리토 재활용을 위한 호밀 식재 식물상 토성회복 가능성

박지은,배범한,주완호,배세달,배은주,Park, Jieun,Bae, Bumhan,Joo, Wanho,Bae, Seidal,Bae, Enjoo 한국지하수토양환경학회 2014 지하수토양환경 Vol.19 No.3

The amount of TPH contaminated soil treated at off-site remediation facilities is ever increasing. For the recycle of the treated-soil on farmlands, it is necessary to restore biological and physico-chemical soil characteristics and to remove residual TPH in the soil by an economic polishing treatment method such as phytoremediation. In this study, a series of experiments was performed to select suitable plant species and to devise a proper planting method for the phyto-restoration of TPH-treated soil. Rye (Secale cereale) was selected as test species through a germination test, among 5 other plants. Five 7-day-old rye seedlings were planted in a plastic pot, 20 cm in height and 15 cm in diameter. The pot was filled with TPH-treated soil (residual TPH of 1,118 mg/kg) up to 15 cm, and upper 5 cm was filled with horticulture soil to prevent TPH toxic effects and to act as root growth zone. The planted pot was cultivated in a greenhouse for 38 days along with the control that rye planted in a normal soil and the blank with no plants. After 38 days, the above-ground biomass of rye in the TPH-treated soil was 30.6% less than that in the control, however, the photosynthetic activity of the leaf remained equal on both treatments. Soil DHA (dehydrogenase activity) increased 186 times in the rye treatment compared to 10.8 times in the blank. The gross TPH removal (%) in the planted soil and the blank soil was 34.5% and 18.4%, respectively, resulting in 16.1% increase of net TPH removal. Promotion of microbial activity by root exudate, increase in soil permeability and air ventilation as well as direct uptake and degradation by planted rye may have contributed to the higher TPH removal rate. Therefore, planting rye on the TPH-treated soil with the root growth zone method showed both the potential of restoring biological soil properties and the possibility of residual TPH removal that may allow the recycle of the treated soil to farmlands.

Protective effect of Korean Red Ginseng extract against Helicobacter pylori-induced gastric inflammation in Mongolian gerbils

Bae, Minkyung,Jang, Sungil,Lim, Joo Weon,Kang, Jieun,Bak, Eun Jung,Cha, Jeong-Heon,Kim, Hyeyoung The Korean Society of Ginseng 2014 Journal of Ginseng Research Vol.38 No.1

Helicobacter pylori-induced gastric inflammation includes induction of inflammatory mediators interleukin (IL)-8 and inducible nitric oxide synthase (iNOS), which are mediated by oxidant-sensitive transcription factor NF-${\kappa}B$. High levels of lipid peroxide (LPO) and increased activity of myeloperoxidase (MPO), a biomarker of neutrophil infiltration, are observed in H. pylori-infected gastric mucosa. Panax ginseng Meyer, a Korean herb medicine, is widely used in Asian countries for its biological activities including anti-inflammatory efficacy. The present study aims to investigate whether Korean Red Ginseng extract (RGE) inhibits H. pylori-induced gastric inflammation in Mongolian gerbils. One wk after intragastric inoculation with H. pylori, Mongolian gerbils were fed with either the control diet or the diet containing RGE (200 mg RGE/gerbil) for 6 wk. The following were determined in gastric mucosa: the number of viable H. pylori in stomach; MPO activity; LPO level; mRNA and protein levels of keratinocyte chemoattractant factor (KC, a rodent IL-8 homolog), IL-$1{\beta}$, and iNOS; protein level of phospho-$I{\kappa}B{\alpha}$(which reflects the activation of NF-${\kappa}B$); and histology. As a result, RGE suppressed H. pylori-induced mRNA and protein levels of KC, IL-$1{\beta}$, and iNOS in gastric mucosa. RGE also inhibited H. pylori-induced phosphorylation of $I{\kappa}B{\alpha}$ and increases in LPO level and MPO activity of gastric mucosa. RGE did not affect viable H. pylori colonization in the stomach, but improved the histological grade of infiltration of poly-morphonuclear neutrophils, intestinal metaplasia, and hyperplasia. In conclusion, RGE inhibits H. pyloriinduced gastric inflammation by suppressing induction of inflammatory mediators (KC, IL-$1{\beta}$, iNOS), MPO activity, and LPO level in H. pylori-infected gastric mucosa.

대학생의 스마트폰 중독에 따른 성지식, 성태도, 성행동의 차이

배소연,김수정,장희진,장으미,이지은,권민 水原大學校 2014 論文集 Vol.28 No.-

The purpose of this study was to define smartphone addiction to their sexual knowledge, attitude and behavior of university student. Data were collected using a questionnaires targeting university students. Date collection was done between July 10, 2014 and July 20, 2014, and collected total 258 copies. Data analyzed using the χ²-test, Fisher's exact test and ANOVA. About 190(73.64%) of the respondents belonged to normal user group, 62(24.81%) to potential risk user group, and high risk user group was 6(2.33%). It was confirmed that the difference of religion(p<.05), daily average smartphone hours(p<.001) and main smartphone purpose of use(p<.05) depending on the smartphone user group. And, there was a significant difference related to sexual intercourse of sexual behavior(p<.05). The result of this study definite that addictive use of smartphone has been determined to affect sexual behavior of university student. Therefore, nursing intervention is required healthy sexual behavior and proper sexual intercourse for the smartphone addiction risk group.

Large-Area Graphene Films by Simple Solution Casting of Edge-Selectively Functionalized Graphite

Bae, Seo-Yoon,Jeon, In-Yup,Yang, Jieun,Park, Noejung,Shin, Hyeon Suk,Park, Sungjin,Ruoff, Rodney S.,Dai, Liming,Baek, Jong-Beom American Chemical Society 2011 ACS NANO Vol.5 No.6

<P>We report edge-selective functionalization of graphite (EFG) for the production of large-area uniform graphene films by simply solution-casting EFG dispersions in dichloromethane on silicon oxide substrates, followed by annealing. The resultant graphene films show ambipolar transport properties with sheet resistances of 0.52–3.11 kΩ/sq at 63–90% optical transmittance. EFG allows solution processing methods for the scalable production of electrically conductive, optically transparent, and mechanically robust flexible graphene films for use in practice.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2011/ancac3.2011.5.issue-6/nn201072m/production/images/medium/nn-2011-01072m_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn201072m'>ACS Electronic Supporting Info</A></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn201072m'>ACS Electronic Supporting Info</A></P>

Decomposition of Paraoxon and Parathion by Amines, HOO^- and OH^- Ions: Reaction Mechanism and Origin of the α-Effect

Bae, Ae-Ri,Lee, Jieun,Um, Ik-Hwan Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.1

The second-order rate constants have been measured spectrophotometrically for the reactions of paraoxon 1 and parathion 2 with a series of alicyclic secondary amines, $OH^-$ and $HOO^-$ ions in $H_2O$ at $25.0{\pm}0.1^{\circ}C$. A linear Br${\o}$nsted-type plot with ${\beta}_{nuc}$ = 0.40 was obtained for the reactions of 1 with amines and $OH^-$. The reaction has been concluded to proceed through a concerted mechanism. $HOO^-$ deviates positively from the linear Br${\o}$nsted-type plot, implying that the ${\alpha}$-effect is operative. The magnitude of the ${\alpha}$-effect ($k_{HOO^-}/k_{OH^-}$) was found to be ca. 55 for the reaction of 1 and 290 for that of parathion 2, indicating that $HOO^-$ is highly effective in decomposition of the toxic phosphorus compounds although it is over 4 $pK_a$ units less basic than $OH^-$. Among the theories suggested as origins of the ${\alpha}$-effect (e.g., TS stabilization through an intramolecular Hbonding interaction, solvent effect, and polarizability effect), polarizability effect appears to be the most important factor for the ${\alpha}$-effect in this study, since the polarizable $HOO^-$ exhibits a larger ${\alpha}$-effect for the reaction of the more polarizable substrate 2.

Skin whitening and anti-wrinkle activities of white rose petal extracts

JIEUN CHOI,EUN SUK AN,YOUNG-HWAN BAN,YESEUL CHA,HAIYU GUO,JI YOUNG LEE,NU RY HAN,EUN SANG CHO,EUN SU JEON,DA WOOM SEO,JUNG-MIN YON,HAE CHEON IM,JAE KWON CHOI,YOON BOK LEE,EHN-KYOUNG CHOI,YUN-BAE KIM 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.08