Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.

Investigation on the Influence of Temperature on Starting Torque of Magnetic Fluid Seal

Jiawei Chen,Decai Li,Du Hao 한국자기학회 2018 Journal of Magnetics Vol.23 No.3

The widest application of magnetic fluid is in magnetic fluid seals. The starting torque varying with temperature of magnetic fluid seal has limited its application in special seal field such as military industry. This paper firstly measure the starting torque of magnetic fluid seals under different temperature conditions from −55 oC to 70 ℃ after holding for 2.5 hours in a closed environment with uniform temperature. And the law of starting torque with temperature change is studied experimentally. The results show that the starting torque of the magnetic fluid seal changes unobvious at 25 ℃ or higher temperature. The starting toque of magnetic fluid seal increases with the decrease of temperature, and the causes of which are analyzed. The results of this study provide experimental and theoretical reference for reducing the staring torque of magnetic fluid seal in low temperature environment.

Long supplementary light duration under same daily light integral provided by white plus blue light-emitting diodes improves quality of greenhouse-grown tomato seedlings

Li Xin,Wang Chunling,Liu Jiawei,Guo Yu,Cheng Fei,Yang Yanjie,Yan Zhengnan 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6

Optimizing light conditions is critical for plant growth and seedling production in greenhouse, especially in seasons when the sunlight is insuffi cient. In the present study, tomato ( Solanum Lycopersicum cv. Fenbeibei) seedlings were grown at two levels of supplementary light duration of 6 h·d − 1 (short) (07:00–10:00 and 15:00–18:00) or 12 h·d − 1 (long) (04:00–10:00 and 15:00–21:00) provided by white light-emitting diodes (LEDs, white long and white short, WL and WS) or combinations of white and blue LEDs (white & blue long and white & blue short, WBL and WBS) with same supplementary daily light integral (at 5.5 mol m − 2 d − 1 ) after sowing, and tomato seedlings grown under sunlight only were set as the control. The per centages of light intensity provided by white and blue LEDs under the WB treatments were 85% and 15%, respectively. The results indicated that tomato seedlings grown under WBL treatment exhibited the lowest plant height and shortest hypocotyl length, which was decreased by 34.6% and 41.8%, respectively, compared with the control. The chlorophyll a, chlorophyll b, and total chlorophyll contents of tomato seedlings grown under WBL were signifi cant higher than those under other treat ments. The root fresh weight, root dry weight, and the stem fi rmness of the greenhouse-grown tomato seedlings under WBL were increased by 21.5%, 41.2%, and 38.6%, compared with those grown under WBS, respectively. The sucrose content of tomato seedlings under WBL was increased by 60.4%, compared with that under WL. In conclusion, 12 h·d − 1 supplementary light provided by combinations of white and blue LEDs led to compact morphological characteristics, superior mechanical properties, and satisfactory growth performance. Our fi ndings provide an available lighting strategy to obtain the desired morphological and quality properties of tomato seedlings grown in the seasons with insuffi cient light.

The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells

Jiawei Lv,Shuang Wu,Renyue Wei,Yan Li,Junxue Jin,Yanshuang Mu,Yu Zhang,Qingran Kong,Xiaogang Weng,Zhonghua Liu 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.3

The clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile genome editing tool with high efficiency. A guide sequence of 20 nucleotides (nt) is commonly used in application of CRISPR/Cas9; however, the relationship between the length of the guide sequence and the efficiency of CRISPR/ Cas9 in porcine cells is still not clear. To illustrate this issue, guide RNAs of different lengths targeting the EGFP gene were designed. Specifically, guide RNAs of 17 nt or longer were sufficient to direct the Cas9 protein to cleave target DNA sequences, while 15 nt or shorter guide RNAs had loss-of-function. Full-length guide RNAs complemented with mismatches also showed loss-of-function. When the shortened guide RNA and target DNA heteroduplex (gRNA:DNA heteroduplex) was blocked by mismatch, the CRISPR/Cas9 would be interfered with. These results suggested the length of the gRNA:DNA heteroduplex was a key factor for maintaining high efficiency of the CRISPR/Cas9 system rather than weak bonding between shortened guide RNA and Cas9 in porcine cells.

Biomimetic functional hydrogel particles with enhanced adhesion characteristics for applications in fracture conformance control

Jiawei Liu,Lin Li,Zhongzheng Xu,Yongpeng Sun,Yining Wu,Caili Dai 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.106 No.-

Fracture conformance control in reservoir can effectively improve water channel to increase crude oil displacementefficiency of subsequent waterflooding. Inspired by the remarkable underwater wet adhesionof mussel byssus, hydrogel particles which can adhere stably on the fracture rock surface in reservoir conditionscould achieve long-lasting reservoir control effect. In this work, the size-controllable biomimeticfunctional hydrogel particles were prepared by mechanical shearing after bulk hydrogel was constructedby catechol-functionalized polyacrylamide and phenolic resin crosslinking agent. The influence of solutionsalinity on the aggregation and adhesion of hydrogel particles was investigated via scanning electronmicroscope (SEM), colloidal probe atomic force microscope (AFM) and quartz crystal microbalance withdissipation (QCM-D). The results showed that hydrogel particles maintained well-dispersed state in lowsalinitywater, while exhibited significant adhesion and adsorption capacity to the silica surface in simulatedreservoir salinity water. This is of great importance to the practical applications that the hydrogelparticles would not show enhanced adhesion to rock surfaces until the reservoir salinity water was met,which was beneficial to the in-depth migration of hydrogel particles to achieve effective deep reservoirprofile control. Furthermore, the visible micro-model was designed and applied to evaluate profile controleffect of hydrogel particles, and the results showed that hydrogel particles could withstand waterflushing and adhere stably to the fracture surface. The waterflooding sweep efficiency was increasedremarkably from 20.3% ± 2.0% to 38.8% ± 2.0%. This work would help better understand the functionmechanism of hydrogel particles in reservoir control and provide novel and efficient method for the practicalapplication in enhanced oil recovery.

Analysis on Seal Capacity of Magnetic Fluid Seal Using Ring Magnet

Jiawei Liu,Decai Li 한국자기학회 2022 Journal of Magnetics Vol.27 No.1

Magnetic fluid seal is one of the most mature applications of magnetic fluid. When the shaft has a large radial runout, the classical pole piece is easily damaged. Due to its small size, the commonly used pole piece structure harbors problems like poor seal performance under a large gap and poor processability. By exploring the distribution law of the magnetic field on the magnet’s surface, we provided theoretical support for the magnetic fluid seal using axially-magnetized ring magnets. New structures for the magnetic fluid seal using axially-magnetized slotted ring magnets and the magnetic fluid seal using radially-magnetized ring magnets were proposed. Then, comparisons were made between the classical magnetic fluid seal and the magnetic fluid seal using ring magnets. The results revealed that the magnetic fluid seal using axially-magnetized slotted ring magnets and the magnetic fluid seal using radially-magnetized ring magnets exhibited a certain seal capacity, which could replace the classical magnetic fluid seal structure.

Experimental nonlinear vibrations of an MRE sandwich plate

Jiawei Zhang,Tanju Yildirim,Gursel Alici,Shiwu Zhang,Weihua Li 국제구조공학회 2018 Smart Structures and Systems, An International Jou Vol.22 No.1

The nonlinear vibration analysis of a magneto-rheological elastomer (MRE) sandwich plate is conducted experimentally. Experiments have been performed in order to construct the frequency-response curves in the vicinity of the fundamental natural frequency of an MRE sandwich plate (plate A) in either the absence or presence of a localised external magnetic field at 3 different geometrical locations, for both small and medium magnetic fields. Furthermore, experiments have also been conducted on a pure aluminium plate (plate B) with an equal thickness to the MRE sandwich plate (plate A) in order to examine the influence of the MRE layer on the nonlinear dynamics of the system. An electrodynamic shaker was used to directly force each system and the displacement at the centre of the plate was measured. Meanwhile, permanent magnets were used to apply a localised magnetic field for the experiments where the MRE sandwich plate was subject to an external magnetic field. It was observed all the MRE systems displayed strong hardening-type nonlinear behaviour, however, with increasing magnetic field this behaviour transitioned to a weak hardening-type nonlinearity.

Numerical Simulation of the Creep Behavior of Beishan Deep Granite Tunnel under the Coupling Thermal -Stress Field

Jiawei Wang,Ju Wang,Zhichao Zhou,Peng Wu,Haoran Sun,Jiale Dou,Nan Li,Xianzhe Duan 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.4

This study conducts a three-dimensional numerical simulation of the creep behavior of deep granite tunnels at 560 meters underground during the coupled thermal-stress process, with an aim to elucidate the effect of the temperature and stress fields on the creep behavior of these deep hard rock tunnels. A 100-h creep period was set, and the mechanical structure of the experimental chamber was accurately replicated at a 1:1 scale, considering the actual mechanical structure of the granite tunnels in the Beishan underground laboratory. The simulation results can demonstrate that: 1) The maximum stress at 90°C and 50°C are 2.86 and 1.91 times than that at 23°C, respectively, demonstrating significant strain accumulation in the deep granite tunnels at the surface. This phenomenon can primarily be attributed to the thermal stress resulted from the coupling between temperature and stress. 2) The maximum creep at 90°C and 50°C is 16 and 3.5 times than that at 23°C. Under the influence of thermal coupling, the creep increases significantly with increasing temperature, indicating that temperature is an important factor influencing creep in granite. 3) Compared with variations in the stress field, the temperature field emerges as the most critical factor influencing granite creep.

Real-time Monitoring for Freezing and Thawing Process of Subgrade in Seasonal Frozen Regions

Li MingBao,Zheng Xian,Zhang JiaWei 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.6

This research shows that there is a cosine changing of the distribution of the temperature field on an annual cycle, and a temperature changing lag exists for the different depth of subgrade. Based on the law of frozen depth of subgrade, the frozen procedures can be divided into four stages. Based on the observation data, the cathode surface temperature is positively correlated with both the frost heave quantity and the frozen depth. In addition, to reduce the observation cost and the measure difficulty, the frost heave quantity can be obtained through the observation of the frozen depth.

Broadband Spectrum Sensing of Distributed Modulated Wideband Converter Based on Markov Random Field

Zhi Li,Jiawei Zhu,Ziyong Xu,Wei Hua 한국전자통신연구원 2018 ETRI Journal Vol.40 No.2

The Distributed Modulated Wideband Converter (DMWC) is a networking system developed from the Modulated Wideband Converter, which converts all sampling channels into sensing nodes with number variables to implement signal undersampling. When the number of sparse subbands changes, the number of nodes can be adjusted flexibly to improve the reconstruction rate. Owing to the different attenuations of distributed nodes in different locations, it is worthwhile to find out how to select the optimal sensing node as the sampling channel. This paper proposes the spectrum sensing of DMWC based on a Markov random field (MRF) to select the ideal node, which is compared to the image edge segmentation. The attenuation of the candidate nodes is estimated based on the attenuation of the neighboring nodes that have participated in the DMWC system. Theoretical analysis and numerical simulations show that neighboring attenuation plays an important role in determining the node selection, and selecting the node using MRF can avoid serious transmission attenuation. Furthermore, DMWC can greatly improve recovery performance by using a Markov random field compared with random selection.