The Influence of Dietary Calcium and Phosphorus Imbalance on Intestinal NaPi-IIb and Calbindin mRNA Expression and Tibia Parameters of Broilers

Li, Jianhui,Yuan, Jianmin,Guo, Yuming,Sun, Qiujuan,Hu, Xiaofei Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.4

A $2{\times}2$ factorial experiment was conducted to study the effect of dietary calcium and non-phytate phosphorus (nPP) imbalance on calbindin and NaPi-IIb mRNA levels in the small intestine and tibia parameters of broiler chicks. One hundred and forty four 1-d-old Arbor Acres male broiler chicks were divided into four treatments consisted of six replicates with six chicks each. The two dietary calcium levels were 1.10% and 0.60%, and two dietary nPP levels were 0.50% and 0.27%. Results showed that a high Ca/nPP ratio diet (4.07:1) significantly depressed feed intake and weight gain of broilers (p<0.05), but a lower Ca:nPP ratio (1.2:1) had no influence (p>0.05). Low-Ca with low-P diet resulted in low tibia minerals and tibia breaking strength of broilers, and all the tibia parameters were further decreased when the dietary ratio of Ca to P was relative higher. Low dietary Ca or P up-regulated the calbindin and NaPi-IIb mRNA expression levels. Low Ca with normal P diet up-regulated duodenal calbindin mRNA expression level to the greatest extent. Low P with a normal Ca diet significantly enhanced NaPi-IIb mRNA expression level to the highest extent. These results suggest that the calbindin and NaPi-IIb mRNA expression were enhanced by the imbalance between dietary Ca and nPP, and their expression were not only influenced by Ca or nPP level, but also the ratio of Ca:nPP.

Expression of FMD Virus-like particles in yeast Hansenula polymorpha and immunogenicity of combine with CpG and Alumimun adjuvant

Jianhui Zhang,Jun Ge,Juyin Li,Jianqiang Li,Yong Zhang,Yinghui Shi,Jiaojiao Sun,Qiongjin Wang,Xiaobo Zhang,Xing-xu Zhao 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.1

Background: Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent. Objectives: The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated. Methods: BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry. Results: The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4+ T cells were observed in mice immunized with VLPs. Conclusions: The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.

Performance Improvement of Finite Time Parameter Estimation with Relaxed Persistence of Excitation Condition

Li Zhao,Jianhui Zhi,Ningning Yin,Yong Chen,Jin Li,Jiaolong Liu 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.2

In this paper, a novel fi nite time parameter estimation method is proposed to solve the parameter estimation problem for a class of linearly parameterized nonlinear systems. The main feature of the proposed method is that the existing method is modifi ed via concurrent learning technique such that the strict persistence of excitation ( PE ) condition on the regression matrix is relaxed to a rank condition on the recorded data. This makes the presented method more practical. Furthermore, the convergence rate is improved signifi cantly by sliding mode technique in fi nite time sense. The simulation results of the existing general nonlinear system illustrate the aforementioned features. Comparison with existing methods from literature proves the eff ectiveness of the proposed method.

Effects of age on intestinal phosphate transport and biochemical values of broiler chickens

Li, Jianhui,Yuan, Jianmin,Miao, Zhiqiang,Guo, Yuming Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.2

Objective: The objective of this experiment was to characterize the mRNA expression profile of type IIb sodium-inorganic phosphate cotransporter (NaPi-IIb) and the biochemical values of serum alkaline phosphatase (AKP), calcium, inorganic phosphorus, tibial ash and minerals of broiler chickens with aging. Methods: A total of 56 one-day-old Arbor Acres male broiler chickens were used. Broiler chickens were weighed and samples were collected weekly from day 1. Results: The result showed that before the growth inflection point, ash, calcium, and phosphorus content in the tibia of broiler chickens increased with growth (before 3 weeks of age), although there were no significant differences in chicks at different ages in the later period of the experiment and weight gain rate was relatively slow at this stage (4 to 6 weeks). NaPi-IIb gene expression in the small intestine in the early growth stage was higher than that in the later growth stage. Expression of calbindin and the vitamin D receptor protein in the intestinal mucosa increased with age in the duodenum and jejunum. Serum AKP activity first increased and subsequently decreased after peaking at 1 week of age, but there was no significant difference after 3 weeks of age. Conclusion: These results show that compared with the early growth stage, the weight-gain rate of broiler chickens in the late growth stage gradually decreased with gradual tibia maturation, along with weaker positive transport of phosphorus in the intestine and reinforced re-absorption of phosphorus in the kidney, which might be the reason that phosphorus requirement in the late growth stage was decreased.

Long Noncoding RNA Expression Profiling During the Neuronal Differentiation of Glial Precursor Cells from Rat Dorsal Root Ganglia

Yunfei Dai,Wei Ma,Tong Zhang,Jinwei Yang,Chenghao Zang,Kuangpin Liu,Xianbin Wang,Jiawei Wang,Zhen Wu,Xingkui Zhang,Chunyan Li,Junjun Li,Xiangpeng Wang,Jianhui Guo,Liyan Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

Long noncoding RNAs (lncRNAs) play important roles in the process of cell fate determination. However, their function and expression profiles have not yet been systematically investigated during the transdifferentiation of glial precursor cells derived from dorsal root ganglia (DRG) in the peripheral nervous system. Our results demonstrated significant differences in gene architecture and expression among the three transcript types (lncRNA, mRNA, and TUCP). Distinct differences in transcript length, exon number, and ORF length were identified between lncRNAs and mRNAs after comparative analysis of their structure and sequence conservation. We found that the upregulated lncRNAs outnumbered the downregulated lncRNAs in glial precursor cells cultured with proBDNF antiserum compared with the levels in glial precursor cells cultured without proBDNF antiserum. By a series of GO and KEGG analyses, we found that the effects of some lncRNAs on their target genes in cis were related to nerve growth factor-induced cell cycle, cell phenotype change, and neuronal differentiation. The qRT-PCR verification results of lncRNAs ENSRNOT00000091991, ENSRNOT00000087717, and LNC_000429 were mostly consistent with the sequencing results. The candidate lncRNAs may be associated with the neuronal transdifferentiation of glial precursor cells. Our study provides the first evidence for a remarkably diverse pattern of lncRNA expression during neuronal differentiation of glial precursor cells from rat DRG, and also provides a resource for lncRNA studies in the field of cell differentiation.

Improved DTCWT-LMS and FastICA Based sEMG Signals Filtering

Li Lin,Wang Jianhui,Fang Xiaoke,Gu Shusheng 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.5

A novel design of dual-tree complex wavelet transform (DTCWT) and fastICA was proposed, aiming at the noise interference and aliasing between multi-channels sEMG signals. Firstly, DTCWT was utilized to decompose signals to different frequency band. Secondly, an improved LMS adaptive filter was designed for filtering sub band noise layer by layer. Finally, fastICA algorithm was introduced to separate crosstalk between channels. Some experiments were carried out to compare the proposed method with other algorithms, and the results showed that the algorithm proposed could filter noise effectively, keep better convergence especially in low signal-to-noise ratio and eliminate crosstalk more thoroughly by fastICA.

Tumor-Associated Macrophages Derived TGF-β-Induced Epithelial to Mesenchymal Transition in Colorectal Cancer Cells through Smad2,3-4/Snail Signaling Pathway

Jianhui Cai,Limin Xia,Jinlei Li,Shichang Ni,Huayu Song,Xiangbin Wu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1

Purpose We investigated the role of tumor-associated macrophages (TAMs) on the epithelial to mesenchymal transition (EMT) of colorectal cancer cells and determined the potential mechanism involved in the metastatic process. Materials and Methods In this study, flow cytometry was used to detect the expression of target proteins. We used transwell assay to evaluate the migration of cancer cells under specific conditions. Using real-time polymerase chain reaction, we examined the expressions of cytokines and EMTrelated markers in mRNA level. Animal assay was performed for analysis in vivo and hematoxylin and eosin was used to visualize the effect of TAMs on tumor metastasis. We also used immunohistochemistry and Western blotting to detect the expression of target proteins. Results Here, we observed enrichment of TAMs in colorectal tumor tissues, resulting in high metastasis in clinical therapy. Moreover, those TAMs could facilitate the EMT progression of colorectal cancer cells, which is induced by the transforming growth factor-β (TGF-β) derived from TAMs, leading to the invasion and migration of cancer cells. Conclusion Our results demonstrated that TAMs contributed the EMT progression through a TGF-β/ Smad2,3-4/Snail signaling pathway, and disrupting this pathway with TGF-β receptor inhibitor could suppress metastasis, readjusting our focus to the connection of TAMs and cancer metastasis.

FPGA Implementation for Binocular Stereo Matching Algorithm Based on Sobel Operator

Jianhui Han,Zhen Wu,Lanying Li,Ying Ji 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.4

Aiming at the low accuracy of stereo matching algorithm caused by the larger gray change, an improved algorithm is proposed. Sobel operator is used to compute the gradient of pixels. Based on the gradient histogram, the adaptive thresholds are derived and the support window is determined automatically. The neighbor pixels are compared with the average of all pixels in the window instead of the center pixel to complete the census transform. The disparity image is abtained through searching the best match point in the left and right images. The hardware implementation with parallel procssing is elaborately designed to improve the capability of the large data processing and computational efficiency based on FPGA. The experimental results show that the improved scheme and hardware structure can obtain disparity map with higher accuracy and stronger robustness.

Loss of Torque on Magnetic Fluid Seals with Rotating-shafts

Jianhui Hu,Meng Zhao,Lu Wang,Jibin Zou,Yong Li 한국자기학회 2017 Journal of Magnetics Vol.22 No.2

The effects of loss of torque on magnetic fluid seals with rotating-shafts and the general difficulty of studying magnetic fluid seals are the focus of this work. The mechanism underlying loss of torque on such seals is analyzed using theoretical methods that show that loss of torque can be affected by several factors, including the velocity of the rotating-shaft, the structure of the sealing device, the characteristics of the magnetic field, and the characteristics of the magnetic fluid. In this paper, a model of the loss of torque is established, and the results of finite element analysis and testing and simulations are analyzed. It is concluded that (ⅰ) the viscosity of the magnetic fluid increased with the intensity of the magnetic field within a certain range; (ⅱ) when the magnetic fluid was saturated, the increase in loss of torque tended to gradually slow down; and (ⅲ) although the axial active length of the magnetic fluid may decrease with increasing speed of the rotating-shaft, the loss of torque increased because of increasing friction.

Electronic transport in benzodifuran single-molecule transistors

Jianhui LIAO,An XIANG,Hui LI,Songjie CHEN,Shi-Xia LIU,Silvio DECURTINS,Meilin BAI,Shimin HOU 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8