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RISS 인기검색어
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- 저자 : Yinghui Shi (검색결과 3 건)
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Yinghui Liu,Yunsu Shi,Yanchun Song,Tianyu Wang,Yu Li 한국식물학회 2010 Journal of Plant Biology Vol.53 No.2
In this paper, a full-length cDNA-encoding cytosolic nicotinamide adenine dinucleotide phosphatedependent isocitrate dehydrogenase was isolated from maize and named ZmICDH. Analysis of the deduced protein sequence revealed that this gene had considerably high homologies with other plant ICDH genes such as those from rice, Arabidopsis and poplar. The gene was transcribed in all tissues tested, with the highest amount of transcript in root. Subcellular localization results indicated that ZmICDH was localized in cytosol. In an attempt to further understand the role of ZmICDH in maize, the functional characterization of the gene was conducted. The results indicated that the expression of ZmICDH was induced by drought and salt stresses. ZmICDH enhanced salt tolerance in transgenic Arabidopsis.
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Xin Liu,Yichen Jiang,Yinghui Zhang,Mingzheng Yu,Hongjun Jiang,Jianhong Xu,Jianrong Shi 한국미생물학회 2019 The journal of microbiology Vol.57 No.8
Dihydroxyacid dehydratase (DHAD), encoded by ILV3, catalyses the third step in the biosynthetic pathway of branchedchain amino acids (BCAAs), which include isoleucine (Ile), leucine (Leu), and valine (Val). Enzymes involved in BCAA biosynthesis exist in bacteria, plants, and fungi but not in mammals and are therefore attractive targets for antimicrobial or herbicide development. In this study, three paralogous ILV3 genes (FgILV3A, FgILV3B, and FgILV3C) were identified in the genome of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). Deletion of FgILV3A alone or combined with FgILV3B or FgILV3C indicated an important role for FgILV3A in BCAA biosynthesis. FgILV3A deletion mutants lost the ability to grow on medium lacking amino acids. Exogenous supplementation of 1 mM Ile and Val rescued the auxotrophy of ΔFgIlv3A, though 5 mM was required to recover the growth defects in ΔFgIlv3AB and ΔFgIlv3AC strains, indicating that FgIlv3b and FgIlv3c exhibit redundant but accessory roles with FgIlv3a in BCAA biosynthesis. The auxotrophy of ΔFgIlv3A resulted in pleiotropic defects in aerial hyphal growth, in conidial formation and germination, and in aurofusarin accumulation. In addition, the mutants showed reduced virulence and deoxynivalenol production. Overall, our study demonstrates that FgIlv3a is crucial for BCAA biosynthesis in F. graminearum and a candidate fungicide target for FHB management.
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Jianhui Zhang,Jun Ge,Juyin Li,Jianqiang Li,Yong Zhang,Yinghui Shi,Jiaojiao Sun,Qiongjin Wang,Xiaobo Zhang,Xing-xu Zhao 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.1
Background: Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent. Objectives: The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated. Methods: BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry. Results: The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4+ T cells were observed in mice immunized with VLPs. Conclusions: The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.
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