Ginsenoside Rb1 exerts neuroprotective effects through regulation of Lactobacillus helveticus abundance and GABA_A receptor expression

Chen, Huimin,Shen, Jiajia,Li, Haofeng,Zheng, Xiao,Kang, Dian,Xu, Yangfan,Chen, Chong,Guo, Huimin,Xie, Lin,Wang, Guangji,Liang, Yan The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1

Background: Ginsenoside Rb1 (Rb1), one of the most abundant protopanaxadiol-type ginsenosides, exerts excellent neuroprotective effects even though it has low intracephalic exposure. Purpose: The present study aimed to elucidate the apparent contradiction between the pharmacokinetics and pharmacodynamics of Rb1 by studying the mechanisms underlying neuroprotective effects of Rb1 based on regulation of microflora. Methods: A pseudo germ-free (PGF) rat model was established, and neuroprotective effects of Rb1 were compared between conventional and PGF rats. The relative abundances of common probiotics were quantified to reveal the authentic probiotics that dominate in the neuroprotection of Rb1. The expressions of the gamma-aminobutyric acid (GABA) receptors, including GABAA receptors (α2, β2, and γ2) and GABAB receptors (1b and 2), in the normal, ischemia/reperfusion (I/R), and I/R+Rb1 rat hippocampus and striatum were assessed to reveal the neuroprotective mechanism of Rb1. Results: The results showed that microbiota plays a key role in neuroprotection of Rb1. The relative abundance of Lactobacillus helveticus (Lac.H) increased 15.26 fold after pretreatment with Rb1. I/R surgery induced effects on infarct size, neurological deficit score, and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) were prevented by colonizing the rat gastrointestinal tract with Lac.H (1 × 10⁹ CFU) by gavage 15 d before I/R surgery. Both Rb1 and Lac.H upregulated expression of GABA receptors in I/R rats. Coadministration of a GABA_A receptor antagonist significantly attenuated neuroprotective effects of Rb1 and Lac.H. Conclusion: In sum, Rb1 exerts neuroprotective effects by regulating Lac.H and GABA receptors rather than through direct distribution to the target sites.

Ginsenoside Rb1 exerts neuroprotective effects through regulation of Lactobacillus helveticus abundance and GABA<SUB>A</SUB> receptor expression

Huimin Chen,Jiajia Shen,Haofeng Li,Xiao Zheng,Dian Kang,Yangfan Xu,Chong Chen,Huimin Guo,Lin Xie,Guangji Wang,Yan Liang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1

Background: Ginsenoside Rb1 (Rb1), one of the most abundant protopanaxadiol-type ginsenosides, exerts excellent neuroprotective effects even though it has low intracephalic exposure. Purpose: The present study aimed to elucidate the apparent contradiction between the pharmacokinetics and pharmacodynamics of Rb1 by studying the mechanisms underlying neuroprotective effects of Rb1 based on regulation of microflora. Methods: A pseudo germ-free (PGF) rat model was established, and neuroprotective effects of Rb1 were compared between conventional and PGF rats. The relative abundances of common probiotics were quantified to reveal the authentic probiotics that dominate in the neuroprotection of Rb1. The expressions of the gamma-aminobutyric acid (GABA) receptors, including GABAA receptors (α2, β2, and γ2) and GABAB receptors (1b and 2), in the normal, ischemia/reperfusion (I/R), and I/RþRb1 rat hippocampus and striatum were assessed to reveal the neuroprotective mechanism of Rb1. Results: The results showed that microbiota plays a key role in neuroprotection of Rb1. The relative abundance of Lactobacillus helveticus (Lac.H) increased 15.26 fold after pretreatment with Rb1. I/R surgery induced effects on infarct size, neurological deficit score, and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) were prevented by colonizing the rat gastrointestinal tract with Lac.H (1×10<SUP>9</SUP> CFU) by gavage 15 d before I/R surgery. Both Rb1 and Lac.H upregulated expression of GABA receptors in I/R rats. Coadministration of a GABAA receptor antagonist significantly attenuated neuroprotective effects of Rb1 and Lac.H. Conclusion: In sum, Rb1 exerts neuroprotective effects by regulating Lac.H and GABA receptors rather than through direct distribution to the target sites.

Polyetheretherketone implants with hierarchical porous structure for boosted osseointegration

Zhiyong Chen,Yu Chen,Yang Wang,JiaJia Deng,Xin Wang,Qingqing Wang,Yuehua Liu,Jiandong Ding,Lin Yu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Good osseointegration is the key to the long-term stability of bone implants. Thermoplastic polyetheretherketone (PEEK) has been widely used in orthopedics; however, its inherent biological inertia causes fibrous tissue to wrap its surface, which leads to poor osseointegration and thus greatly limits its clinical applications. Methods Herein, we developed a facile yet effective surface modification strategy. A commonly used sulfonation coupled with “cold pressing” treatment in the presence of porogenic agent formed a three-dimensional hierarchical porous structure on PEEK surface. Subsequently, the effects of porous surface on the in vitro adhesion, proliferation and differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs) were evaluated. Finally, the osteoinduction and osseointegration of surface-porous PEEK implant were examined in the rat distal femoral defect model. Results In vitro results showed that the surface modification did not significantly affect the mechanical performance and cytocompatibility of PEEK substance, and the porous structure on the modified PEEK substrate provided space for cellular ingrowth and enhanced osteogenic differentiation and mineralization of BMSCs. In vivo tests demonstrated that the surface-porous PEEK implant could effectively promote new bone formation and had higher bone-implant contact rate, thereby achieving good bone integration with the surrounding host bone. In addition, this modification technique was also successfully demonstrated on a medical PEEK interbody fusion cage. Conclusion The present study indicates that topological morphology plays a pivotal role in determining implant osseointegration and this facile and effective modification strategy developed by us is expected to achieve practical applications quickly.

Ginsenoside Rb1 exerts neuroprotective effects through regulation of Lactobacillus helveticus abundance and GABAA receptor expression

Huimin Chen,Jiajia Shen,Haofeng Li,Xiao Zheng,Dian Kang,Yangfan Xu,Chong Chen,Huimin Guo,Lin Xie,Guangji Wang,Yan Liang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1

Background: Ginsenoside Rb1 (Rb1), one of the most abundant protopanaxadiol-type ginsenosides, exertsexcellent neuroprotective effects even though it has low intracephalic exposure. Purpose: The present study aimed to elucidate the apparent contradiction between the pharmacokineticsand pharmacodynamics of Rb1 by studying the mechanisms underlying neuroprotective effects ofRb1 based on regulation of microflora. Methods: A pseudo germ-free (PGF) rat model was established, and neuroprotective effects of Rb1 werecompared between conventional and PGF rats. The relative abundances of common probiotics werequantified to reveal the authentic probiotics that dominate in the neuroprotection of Rb1. The expressionsof the gamma-aminobutyric acid (GABA) receptors, including GABAA receptors (a2, b2, and g2) andGABAB receptors (1b and 2), in the normal, ischemia/reperfusion (I/R), and I/RþRb1 rat hippocampus andstriatum were assessed to reveal the neuroprotective mechanism of Rb1. Results: The results showed that microbiota plays a key role in neuroprotection of Rb1. The relativeabundance of Lactobacillus helveticus (Lac.H) increased 15.26 fold after pretreatment with Rb1. I/R surgeryinduced effects on infarct size, neurological deficit score, and proinflammatory cytokines (IL-1b, IL-6, andTNF-a) were prevented by colonizing the rat gastrointestinal tract with Lac.H (1 109 CFU) by gavage 15d before I/R surgery. Both Rb1 and Lac.H upregulated expression of GABA receptors in I/R rats. Coadministrationof a GABAA receptor antagonist significantly attenuated neuroprotective effects of Rb1 and Lac.H. Conclusion: In sum, Rb1 exerts neuroprotective effects by regulating Lac.H and GABA receptors ratherthan through direct distribution to the target sites.

Experimental and numerical simulation study on fracture properties of self-compacting rubberized concrete slabs

Jiajia Wang,Xudong Chen,Jingwu Bu,Shengshan Guo 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.24 No.4

The limited availability of raw materials and increasing service demands for pavements pose a unique challenge in terms of pavement design and concrete material selection. The self-compacting rubberized concrete (SCRC) can be used in pavement design. The SCRC pavement slab has advantages of excellent toughness, anti-fatigue and convenient construction. On the premise of satisfying the strength, the SCRC can increase the ductility of pavement slab. The aim of this investigation is proposing a new method to predict the crack growth and flexural capacity of large-scale SCRC slabs. The mechanical properties of SCRC are obtained from experiments on small-scale SCRC specimens. With the increasing of the specimen depth, the bearing capacity of SCRC beams decreases at the same initial crack-depth ratio. By constructing extended finite element method (XFEM) models, crack growth and flexural capacity of large-scale SCRC slabs with different fracture types and force conditions can be predicted. Considering the diversity of fracture types and force conditions of the concrete pavement slab, the corresponding test was used to verify the reliability of the prediction model. The crack growth and flexural capacity of SCRC slabs can be obtained from XFEM models. It is convenient to conduct the experiment and can save cost.

Hydroxide MgSn(OH)6: A Promising New Photocatalyst for Methyl Orange Degradation

Jiajia Tao,Zhaoqi Sun,Miao Zhang,Gang He,Xiaoshuang Chen 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.4

Highly crystalline hydroxide MgSn(OH)6 (MHS) polyhedral particleswere synthesized by changing reaction time (10, 15 and 20 h) in ahydrothermal process. The structural and morphological poperties ofobtained samples were characterized by X-ray diffraction (XRD),scanning electron microscopy (SEM), and UV-vis diffuse reflectancespectroscopy (DRS). The photocatalytic activity of the MHS wasfurther evaluated by the degradation of methyl orange (MO) underultraviolet (UV) light illumination. Compared with commercial TiO2(Degussa P25), the MHS prepared for 15 h showed similar degradationefficiency of methyl orange (MO), mainly due to its higher specificsurface area (55 m2g−1) and better optical properties.

Facile preparation and characterization of tough poly(vinyl alcohol) organohydrogels with low friction and self-cleaning properties

Jia Yang,Jiajia Hao,Chen Tang,Yaxin Guo,Mingxin Guo,Zhipeng Li,Shuzheng Liu,Hui Yu,Gang Qin,Qiang Chen 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.116 No.-

Although many hydrogels have been applied to wearable sensors, it is still challenging to simultaneouslyrealize hydrogels with optical transparency, superior mechanical properties, excellent sensing performance,and anti-freezing by using inexpensive raw materials and an easy preparation process. Herein,using ethylene glycol/H2O (EG/ H2O) as a solvent, poly(vinyl alcohol)/EG organohydrogel (PVA/EGOHG) was prepared by a simple heating and frozen-thawing method. Owing to the multifunctionalityof EG (i.e., physical cross-linker, anti-freezer and co-solvent), PVA/EG OHG demonstrated excellent integratedproperties, including high strength, high toughness, and anti-freezing performances. Besides, PVA/EG OHG also showed low friction, self-cleaning, and frost resistance properties. After the introduction ofLiCl, ionically conductive PVA/EG @LiCl organohydrogel was served as a self-cleaning strain sensor, whichcould be long-term stable to detect the motions of human under room and low temperatures. The studyprovides to further understanding of the organohydrogel, which will help us design next-generationhigh-performance organohydrogels.

Welding Performance of Low-Yield-Strength Steel Shear Panel Dampers Under Large Plastic Deformation

Chaofeng Zhang,Shixi Chen,Junhua Zhao,Xuchuan Lin,Lingxin Zhang,Jiajia Zhu,Weili Wang 한국강구조학회 2021 International Journal of Steel Structures Vol.21 No.6

Characterized by large plastic deformation and energy-dissipation capacity, low-yield-strength steel shear panel dampers (LYSPDs) are widely used as energy-dissipating members in seismic engineering. However, the eff ect of welding on the properties of LYSPDs remains unclear. Hence, in this study, we investigated the matching performance between low-yieldstrength steel (LYS) and two diff erent welding materials. Subsequently, the quality of welding between LYS and dissimilar metals was investigated. Furthermore, the fracture characteristics of the welding seam of LYS under large plastic deformation were explored. Finally, the welding performance of LYSPDs under large plastic deformation was verifi ed under cyclic loading. The results of this study are signifi cant in terms of the eff ect of welding on the properties of LYSPDs.

Biotechnological Advances and Trends in Engineering Trichoderma reesei towards Cellulase Hyperproducer

Hao Fang,Chaofeng Li,Jiajia Zhao,Chen Zhao 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.4

Cellulase has the biggest contribution to the high production costs of lignocellulose bioconversion and the substantial decrease of its production cost is the key to the commercialization of lignocellulosic biorefineries. Trichoderma reesei has the most robust cellulase among the candidates, which therefore is widely used for cellulase production in industry. This is not because of the size of its cellulase gene pool but its prodigious cargo of cellulase productivity. Still, T. reesei cellulase falls far short of perfection in real-world applications, especially for the composition. This review summarized the biotechnological advances in engineering T. reesei for enhanced cellulase production. Meanwhile, we proposed innovative ideas of systematically optimizing cellulase composition at the transcriptional level and improving cellulase production at the regulation level. Efficient genome editing is essential to achieving that target. Thus, the developments of the tools of multiple gene manipulations were discussed in detail here. This review provides ideas and/or inspirations to the future researches on T. reesei cellulase.

QoE-aware Energy Efficiency Maximization Based Joint User Access Selection and Power Allocation for Heterogeneous Network

( Shiyu Ji ),( Liangrui Tang ),( Chen Xu ),( Shimo Du ),( Jiajia Zhu ),( Hailin Hu ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.10

In future, since the user experience plays a more and more important role in the development of today’s communication systems, quality of experience (QoE) becomes a widely used metric, which reflects the subjective experience of end users for wireless service. In addition, the energy efficiency is an increasingly important problem with the explosive growth in the amount of wireless terminals and nodes. Hence, a QoE-aware energy efficiency maximization based joint user access selection and power allocation approach is proposed to solve the problem. We transform the joint allocation process to an optimization of energy efficiency by establishing an energy efficiency model, and then the optimization problem is solved by chaotic clone immune algorithm (CCIA). Numerical simulation results indicate that the proposed algorithm can efficiently and reliably improve the QoE and ensure high energy efficiency of networks.