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흰쥐 생체시료 중 5-플루오로우라실 및 테가푸르의 안정성
장지현,박종국,강진형,정석재,심창구,구효정 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.3
5-Fluorouracil (5-FU) is an antimetabolite anticancer agent active against many types of solid tumors. Tegafur (TF), a prodrug of 5-FU, is frequently used in combination with uracil as dihydropyrimidine dehydrogenase (DPD) inhibitory fluoropyrimidine. We studied the stability of 5-FU and TF in biological fluids of rats and determined their bioavailability (BA) and excretion into bile, and urine. The drug concentrations were analyzed by an HPLC method. At room temperature, there was a 14-30% decrease in the concentration of 5-FU and TF in bile, urine, and plasma specimen at 10 and 100 μg/ml over 240 min. No significant difference was noted among the sample types or between two different concentrations of 10 and 100 μg/ml. The decrease in drug concentration was significantly less in samples kept on ice (6-12%) for both drugs. These data indicate that biological fluid samples containing 5-FU or TF in plasma, urine, or bile should be placed on ice during the sample collection. Following these storage guidelines, samples were collected after administration 50 ㎎/㎏ of each drug via i.v. or oral route. BA was 1.5 folds greater for TF (60%) than that of 5-FU (42%). Approximately 0.52 and 3.3% of the i.v. doses of 5-FU and TF was excreted into bile, respectively. Renal clearance of 5-FU was about 16% of its total body clearance. These results suggest that instability of 5-FU and TF in biological fluids should be considered in pharmacokinetic or pharmacogenomic studies.
박히준,이지숙,이재동,김남재,표지희,강전모,최일환,김수영,심범상,이제헌,임사비나 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-
Objectives : Cinnamomi Ramulus (CR), the young twing of Cinnamomi loureirii nees, has been used for treating symptoms related to pain. rheumatic arthritis and inflammation in Korean herb medicine. This study was carried out to investigate the anti-inflammatory effect of CR in vivo and in vitro. Methods : Extracts of CR were prepared and the chemical components of the extracts were examined by gas chromatography-mass spectrometry (GC-MS). The extracts were administrated to the rat paw edema model induced by carrageenan to evaluate the anti-inflammatory effect of CR. The expressions of nitric oxide (NO). prostaglandin E2(PGE2), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 were also quantified in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages to survey the effect of CR in vitro. The main components were cinnamaldechyde and coumarin. Results : We examined the anti-inflammatory activity of the 80% ethanol extract of Cinnamomi Ramulus in vivo by using carrageenan-induced rat paw edema model. Maximum inhibition of 54.9% was noted at the dose of 1000㎎/㎏ after 2 hours of drug administration in carrageenan-induced rat paw edema and this showed a potent anti-inflammatory effect. Conclusions : The results showed that Cinnamomi Ramulus suppressed dose-dependently LPS-induced NO production in RAW 264.7 macrophages and also decreased iNOS protein expression. Cinnamomi Ramulus also showed a significant inhibitory effect in LPS-induced PGE2 production and COX-2 expression.
인체 간 Microsome에서의 우슬 추출물의 Cytochrome P450 약물 대사효소에 대한 억제작용
김경아,이지숙,박히준,김진우,김창주,심인섭,한승무,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-
Objective : Achyranthes bidentata radix (Usul) has been used as anti-arthritic, antiallergic, antidiuretic and so on Recently extracts of Achyranthes bidentata radix have shown anti-inflammatory and cancer preventive effects in vitro and in vivo. Methods : We therefore evaluated the inhibitory potential of ehtanol extracts of Achyranthes bidentata radix on cytochrome P450(CYP) isoforms-catalyzecd reactions, which relate to causes of cancer and inflammation, including CYP1A2, CYP2C9, CYP2C19, CYP2E1, CYP2D6, CYP2C8, and CYP3A4, using human liver microsomal preparations. Results : The extracts showed weak or negligible inhibitory effects on CYP2C9-catalyzed (S)-warfarin 7-hydroxylation, CYP2C19-catalyzed S-mephenytoin 4-hydroxylation, and CYP2D6-catalyzed dextromethorphan O-demethylation with each IC50 over 1750 g/㎖, respectively. However, it showed relatively significant inhibitory effect on CYP1A2-catalyzed phenacetin O-deethylation and CYP2E1-catalyzed chloizoxazone 6-hydroxylation with IC50s of 970.5 g/㎖ and 821.4 g/㎖, respectively. Conclusions : These results suggest that extracts of Achyranthes bidentata radix have inhibitory effects on CYP-catalyzed reactions, expeciallyCYP1A2 and CYP2E1, in human liver microsomes. These effects appear to relate to anti-inflammatory and cancer prevention following decrease of reactive oxygen species formed by CYP, especially CYP1A2 and CYP2E1, by Achyranthes bidentata radix. However, further evaluation is necessary to demonstrate and to confirm its effects in human.
박히준,이제현,김수영,심범상,구헌종,강전모,최일환,이재동,김남재,이지숙,임사비나 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-
Objective : The use of herbal therapy is becoming an increasingly attractive approach for the treatment of various inflammatory disorders. The Alpiniae officinari Rhizoma is popular in Aisa as a traditional herbal medicine. Alpiniae officinari Rhizoma is a species of the ginger family(Zingiberacease). Method : This study was performed to investigate the anti-inflammatory effect of Alpiniae officinari Rhizoma extract by the methods of "carrageenan induced paw edema" and "Lipopolysaccharide-induced inflammatory mediators in mouse macrophage RAW 264.7 cells". Result : We suggest that Alpiniae officinari Rhizoma extract decreased paw volume induced by plantar injection of carrageenan. Also Alpiniae officinari Rhizoma extract inhibited nitric oxide, prostaglandin E₂production and induced nitric oxide synthase, cyclooxygenase-2 protein expression in Mouse macrophage RAW 264.7 cells stimulated with lipopolysaccharide. Conclusion : This study shows that Alpiniae officinari Rhizoma extract seems to have anti-inflammatory effect by inhibition of nitric oxide, prostaglandin E_(2) production and nitric oxide synthase, cyclooxygenase-2 protein expression.
Shim, Ji-Suk,Lee, Jeong-Yol,Choi, Yeon-Jo,Shin, Sang-Wan,Ryu, Jae-Jun The Korean Academy of Prosthodonitics 2015 The Journal of Advanced Prosthodontics Vol.7 No.1
PURPOSE. This study aimed to discover a way to increase the bond strength between bis-acryl resins, using a comparison of the shear bond strengths attained from bis-acryl resins treated with light curing, pressure, oxygen inhibition, and heat. MATERIALS AND METHODS. Self-cured bis-acryl resin was used as both a base material and as a repair material. Seventy specimens were distributed into seven groups according to treatment methods: pressure - stored in a pressure cooker at 0.2 Mpa; oxygen inhibition- applied an oxygen inhibitor around the repaired material,; heat treatment - performed heat treatment in a dry oven at $60^{\circ}C$, $100^{\circ}C$, or $140^{\circ}C$. The shear bond strength was measured with a universal testing machine, and the shear bond strength (MPa) was calculated from the peak load of failure. A comparison of the bond strength between the repaired specimens was conducted using one-way ANOVA and Tukey multiple comparison tests (${\alpha}$=.05). RESULTS. There were no statistically significant differences in the shear bond strength between the control group and the light curing, pressure, and oxygen inhibition groups. However, the heat treatment groups showed statistically higher bond strengths than the groups treated without heat, and the groups treated at a higher temperature resulted in higher bond strengths. Statistically significant differences were seen between groups after different degrees of heat treatment, except in groups heated at $100^{\circ}C$ and $140^{\circ}C$. CONCLUSION. Strong bonding can be achieved between a bis-acryl base and bis-acryl repair material after heat treatment.
디지털 인상채득을 이용한 소구증을 가진 환자의 전악 임플란트 수복
심지석(Ji Suk Shim),류재준(Jae Jun Ryu) 대한치과의사협회 2018 대한치과의사협회지 Vol.56 No.11
This clinical case highlights the failure of long length implants, and the prosthodontic procedures necessary to rehabilitate the maxillary dentition of a patient with microstomia. The integrated digital technology of intra-oral scanning, computer-aided design, and three-dimensional printing can provide an alternative method to make conventional impressions for patients with microstomia who cannot insert the appropriate tray in their mouths.
심호영 ( Ho Young Shim ),이교석 ( Kyo Suk Lee ),이동성 ( Dong Sung Lee ),전대성 ( Dae Sung Jeon ),박미숙 ( Mi Suk Park ),신지수 ( Ji Su Shin ),이연경 ( Yun Koung Lee ),구지원 ( Ji Won Goo ),김수빈 ( Soo Bin Kim ),송승근 ( Seong Ge 한국환경농학회 2014 한국환경농학회지 Vol.33 No.3
BACKGROUND: Increase in application of liquid pigmanure(LPM) in agriculture as nutritional source hasbecome a social issue due to its influence on water quality. Also, proper application methods have not been developedwith respect to indigenous properties of LPM and soilphysical properties. Therefore, we conducted thisexperiment to observe the infiltration characteristics anddistribution of dissolved organic compounds of LPM insoils having different soil textures. METHODS AND RESULTS: To do this experiment, wecollected three different soils and LPM. We analyzed thephysical and chemical properties of both soils and LPM todetermine the dilution ratios of LPM. The LPM diluted to 4different ratios with distilled water was applied to the top ofsoil column. Infiltration rates were observed by time anddepth until the amount of effluent collected from the bottomof the soil columns were stabilized while maintaining thehydraulic head 3 cm above the soil column. The resultsshowed that infiltration rates increased with increasingdilution ratios in the order of sandy, loamy sand, and sandyloam> The time required to reach steady state was increasedwith decreasing sand contents clay. CONCLUSION: The size and amount of the dissolvedorganic compounds in LPM that can determine theefficiency as fertilizer and environmental problems asnonpoint pollution source in water quality have not beeninvestigated with respect to behavior and transport of themin soil. Therefore, it requires further research how we canproperly apply LPM as valuable fertilizer substitute forinorganic fertilizers.
Profiling of Fermentative Metabolites in Kimchi: Volatile and Non-volatile Organic Acids
Shim, Soon-Mi,Kim, Ji Yun,Lee, Sang Mi,Park, Jin-Byung,Oh, Sea-Kwan,Kim, Young-Suk The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.4
Gas chromatograph/mass spectrometry (GC/MS) analysis was carried out to determine both the volatile and non-volatile organic acids in kimchi during 60 days of fermentation at $10^{\circ}C$. Principal component analysis (PCA) was applied to differentiate the pre-defined organic acids and lactic acid bacteria (LAB) during fermentation. Acetic acid was observed as dominant, which was vigorously produced until the middle of fermentation. Lactic acid was the major non-volatile organic acid in the kimchi and was produced throughout fermentation. In contrast, malic acid content decreased sharply at the initial stage of fermentation. Colony forming units of LAB in the kimchi, such as Leuconostoc, Lactobacilli, Pediococci, and Streptococci, were measured on selective media. Populations of Leuconostoc and Lactobacilli increased exponentially over 7 days of fermentation, indicating acetic acid and lactic acid were mainly produced by Leuconostoc and Lactobacilli. PCA demonstrated that acetic acid, propionic acid, lactic acid, butanoic acid, malic acid, Leuconostoc, and Lactobacilli were major components that differentiated the kimchi according to fermentation time.