Analysis of fucosylation in liver-secreted N-glycoproteins from human hepatocellular carcinoma plasma using liquid chromatography with tandem mass spectrometry

Ji, E. S.,Hwang, H.,Park, G. W.,Lee, J. Y.,Lee, H. K.,Choi, N. Y.,Jeong, H. K.,Kim, K. H.,Kim, J. Y.,Lee, S. Springer Science + Business Media 2016 Analytical and Bioanalytical Chemistry Vol.408 No.27

<P>Fucosylation of N-glycoproteins has been implicated in various diseases, such as hepatocellular carcinoma (HCC). However, few studies have performed site-specific analysis of fucosylation in liver-secreted proteins. In this study, we characterized the fucosylation patterns of liver-secreted proteins in HCC plasma using a workflow to identify site-specific N-glycoproteins, where characteristic B- and/or Y-ion series with and without fucose in collision-induced dissociation were used in tandem mass spectrometry. In total, 71 fucosylated N-glycopeptides from 13 major liver-secreted proteins in human plasma were globally identified by LC-MS/MS. Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. Of the fucosylated N-glycopeptides, bi- and tri-antennary glycoforms were the most common ones identified in liver-secreted proteins from HCC plasma. Therefore, we suggest that this analytical method is effective for characterizing fucosylation in liver-secreted proteins.</P>

노인의 약물 지식 정도와 약물 사용실태 및 약물 오·남용 행위

구예나,김민경,김진윤,배윤,서민혜,정은혜,지수현,차미나,Choi, Jenny 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: The purpose of this study was to provide basic data for use in the upgrade of healthcare for the elderly and to provide guidance toward educating the elderly in the proper use of medications by determining the level of drug knowledge, current use, and adherence by the elderly. Method: The participants of this study were selected using the convenient sampling method. Participants of this study comprised of 160 elderly who were aged 65 or older, who were participants to the elderly municipal welfare center in Seoul. The instrument used for this study was a structured questionnaire (Han,2011). Collected data were analyzed using the SPSS20.0 program with descriptive statistics, t-test, ANOVA, and Pearson’s correlation coefficient. Result: The mean score for drug knowledge was 7.6±2.9 and drug misuse was 4.7±2.8. There were significant differences about drug knowledge according to the following: level of education (F=8.053 p=.000), and spouse (t=2.14 p=.034). There were significant differences between drug misuse and monthly income (F=2.167 p=.040). There was no statistically significant relationship between drug knowledge and drug misuse (r=-.087, p=.271). Conclusion: A replication study based on broader region and in-depth research is needed. The development of the customized education protocol considering various levels of education, income, spouse and physical condition are needed. Further research is suggested to find out the status of medication education for the elderly by nurses.

100X110μm² micro mirror array의 제작과 실험

지창현,정석환,신종우,김용권,최범규,안세진 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

A 100 x110μm^(2) aluminum micro structure is fabricated using thick photoresist as sacrificial layer and mold for nickel electroplating. The micro structure is composed of aluminum plate, hinge, nickel support post, and address electrode. The aluminum plate is overhung about 10 μm above the silicon substrate supported by two nickel posts. The hinge connects the aluminum plate and the support post and works as a torsional spring. We used thick PR as 10 μm thick sacrificial layer and nickel electroplating mold and electroplated nickel for 10 μm high post. The aluminum plate is actuated by electrostatic force between the aluminum plate and the address electrode. The aluminum micro structure is finally released by reactive ion etching (RIE) using O_(2). The micro mirror is actuated at 35V.

임부의 연령에 따른 보완대체요법에 관한 인식 태도 경험 비교 연구

김연진,김지연,노경민,신혜리,유예지,이지민,장희조,정아인,최소정,강숙정,이향연,민혜영 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: This study aims to test if the age of pregnant women affects the perception, experience, and attitude towards complementary alternative therapy (CAT), by investigating the perception, experience, and attitude of pregnant women under the age of 35, and above 35 (Advanced Maternal Age). The study will 1) find the general characteristics of pregnant women under and over the age of 35 2) compare the perception, behavior, and experience of pregnant women under and over the age of 35, and 3) find the comparison of the perception, behavior, and experience according to the general characteristics of pregnant women under and over the age of 35. Method: The subjects of this study are composed of 44 women under the age of 35 and 44 women above the age of 35, totaling 88 pregnant women visiting midwiferies, obstetrics and gynecology, and baby fairs in Seoul and Gyeong-Gi area. Data for this study was collected from November 28, 2015 to January 6, 2016 for 40 days. The collected data analyzed mean, standard deviation, t test, ANOVA, and chi-square by using SPSS 23.0. Result Results show that there is a significant difference in the perception, attitude, and experience of the two-sample groups in the use of CAT. The question of “what is most necessary when applying CAT in a hospital clinical environment” for the perception criteria came out with the results of t=9.980, p<.05. For the attitude, results showed that the answers of pregnant women under the age of 35 displayed a more positive attitude towards CAT. (t=2.421, p<.05) Lastly, in the experience section, pregnant women under the age of 35 showed more CAT use than the other sample group. Conclusion Results have shown that there is a difference in the perception, behavior, and experience between the two sample groups. Therefore further research on the efficacy and the adverse effects of CAT needs to be done.

남자대학생의 정신건강, 부모양육태도, 애착이 도박중독에 미치는 영향

김혜진,박재희,유지원,이수현,이지은,임소정,장우주,지연주,최민정,김석선,박향진 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Objectives: The purpose of this research was to identify the effect of mental health, parenting attitude and attachment on gambling addiction severity among male college students. Methods: Subjects of the research were 292 students recruited from colleges in Seoul, Korea. The questionnaires were composed of CPGI, DASS-21, PBI, ECR-R. The data was statistically analyzed by Chi-square, ANOVA, Scheffe test and Pearson’s correlation. Results: The proportion of moderate-risk gambling and high-risk gambling group was reported 28.1%. There a statistically significant difference between gambling addiction severity groups in Anxiety(F=3.19, p<.05). Statistically significant linear relationship was found between Anxiety and gambling addiction score(r=.15, p<.05). There was statistically significant difference between gambling addiction severity groups in Caring(F=4.74, p<.01). Statistically significant linear relationship was found between Caring and gambling addiction score(r=-2.14, p<.01). There was statistically significant difference between gambling addiction severity groups in Overprotection(F=4.74, p<.01). Statistically significant linear relationship was examined between Overprotection and gambling addiction score(r=.20, p<.01). Significant difference was examined between gambling addiction severity in Attachment Anxiety(F=3.76, p<.05). Attachment Anxiety showed significant linear relationship with gambling addiction score(r=-.17, p<.01). Significant differences were examined between gambling addiction severity in Attachment avoidance (F=3.46, p<.05). Attachment avoidance showed no significant relationship with gambling addiction score(r=-.09, p=.12). Conclusions: This study showed the effect of mental health, parenting attitude and attachment on gambling addiction severity among male college students. Based on the result, it is recommend for nurses to develop intervention to prevent gambling addiction among college students by lowering anxiety, stabilizing attachment through desirable caring attitude from parents.

In situ gamma-ray spectrometry in the environment using dose rate spectroscopy

Ji, Y.Y.,Kim, C.J.,Chung, K.H.,Choi, H.Y.,Lee, W.,Kang, M.J.,Park, S.T. Pergamon 2016 Radiation physics and chemistry Vol.119 No.-

<P>In order to expand the application of dose rate spectroscopy to the environment, in situ gamma-ray spectrometry was first conducted at a height of 1 m above the ground to calculate the ambient dose rate and individual dose rate at that height, as well as the radioactivity in the soil layer for the detected gamma nuclides from the dose rate spectroscopy. The reliable results could be obtained by introducing the angular correction factor to correct the G-factor with respect to incident photons distributed in a certain range of angles. The intercomparison results of radioactivity using ISOCS software, an analysis of a sample taken from the soil around a detector, and dose rate spectroscopy had a difference of < 20% for Pb-214, Bi-214, Ac-228, Bi-212, Tl-208, and K-40, except for Pb-212 with low-energy photons, that is, < 300 key. In addition, the drawback of using dose rate spectroscopy, that is, all gamma rays from a nuclide should be identified to accurately assess the individual dose rate, was overcome by adopting the concept of contribution ratio of the key gamma ray to the individual dose rate of a nuclide, so that it could be accurately calculated by identifying only a key gamma ray from a nuclide. (C) 2015 Elsevier Ltd. All rights reserved.</P>

Older Adults in an Aging Society and Social Computing: A Research Agenda

Ji, Y. G.,Choi, J.,Lee, J. Y.,Han, K. H.,Kim, J.,Lee, I. K. Taylor Francis 2010 INTERNATIONAL JOURNAL OF HUMAN-COMPUTER INTERACTIO Vol.26 No.11

The Measurement of the hardness for Korean foods related with the masticatory function

Choi, Yun-Hwa Park, Ji-Hyeon Ahn, Sun-Ha Lee, Ji-Young 대한임상예방치과학회 2008 International Journal of Clinical Preventive Denti Vol.4 No.1

Objectives : This study set out to examine the solidity of major foods consumed by ederly in Korea, identify the kinds of food according to categories, calculate correlations between the food and number of remaining teeth, and provide some guidelines to maintain, manage, and preserve the healthy state of natural teeth. Methods : The authors has experimented on the compressive strength for 10 kinds of favorite foods for Korean, in order to classify the hardness of the representative foods, among the various foods selected from the national survey data of favorite foods for Korean in the year 2005. 1.0㎝, 1.0㎝, & 0.5㎝ in height sized samples were produced with each food and measured the compressive strength with the multitest-i, with the condition of 0.35㎝ distance and 0.3㎝/㎜ of the speed. The peak value was investigated. Results: The compressive strength was estimated as 192.3fg in Tofu, 204.3 gf in boiled rice, 868.7 gf in white of boiled egg, 1089.3 gf in biscuits, 14160. gf in cabbage kimchi, 1843.0 gf in beef meet, 2790.0 gf in cubed white-radish kimchi, 3083.2 in apple, 4360.0 in peanuts, 4820.0 in bean 11500.0 gf in dried squid and 25210.0 gf in candy, 10 categories according to hardness were as water/milk as degree 0, Tofu as degree 1, boiled rice as degrees 2, white of boiled egg as degree 3, biscuit and cabbage kimchi as degree 7, peanuts or bean as degree 8, dried squid as degree 9 and candy as degree 10. Conclusion: It was recommended for the further study on the relationship between the numbers of the residual natural teeth and the chewing ability of each degree of the food, in order to estimate the oral health goals for chewing ability and and the natural teeth for aged people.

Dry Eye-Induced CCR7+CD11b+ Cell Lymph Node Homing Is Induced by COX-2 Activities

Ji, Y. W.,Seo, Y.,Choi, W.,Yeo, A.,Noh, H.,Kim, E. K.,Lee, H. K. Association for Research in Vision and Opthalmolog 2014 Investigative Ophthalmology & Visual Science Vol.55 No.10

Removal of nitrate and ammonium ions from livestock wastewater by hybrid systems composed of zero-valent iron and adsorbents

Ji, M. K.,Ahn, Y. T.,Ali Khan, M.,Abou-Shanab, R.,Cho, Y.,Choi, J. Y.,Je Kim, Y.,Song, H.,Jeon, B. H. Taylor Francis 2011 Environmental Technology Vol.32 No.16