Effective Combination of Agrobacterium tumefaciens Strains and Ti Plasmids for the Construction of Plant Vector System

Kim, Mi Suk,Park, Jeong Ku,Eum, Jin Seong,Sim, Woong Seop 한국식물학회 1996 Journal of Plant Biology Vol.39 No.3

The purpose of this study is to obtain the most efficient combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of dicotyledonous plant vector system. Ti plasmid-curing A. tumefaciens A136 and KU12C3 were transformed with four kinds of Ti plasmids, pTiBo542, pTiA6, pTiKU12 and pTiAch5, respectively. The stems of 28 species of dicotyledonous plants were then inoculated with these transformants and examined for crown gall formation. The different combination of A. tumefaciens strains and Ti plasmids showed quite a difference in terms of the crown gall formation. Agrobacterium strains A136 and KU12C3 have a same plant host range in case that both strains barbour the same kind of Ti plasmid, pTiBo542 or pTiAch5. However, the above-mentioned both strains have quite different host range in the event of containing the same Ti plasmid, pTiKU12 or pTiA6. In case that KU12C3 contains pTiA6 or pTiKU12, this strain has a wider plant host range than A136. The plant host range of pTiBo542 is the widest, followed by pTiA6, pTiKU12 and pTiAch5. Twelve plants among 28 tested plants are not transformed by any virulent Agrobacterium strains used in this study. In conclusion, A. tumefaciens KU12C3 and A136 harboring pTiBo542 showed the widest host range for transforming dicotyledonous plants. Also, it was acertained that the host range of Ti plasmids is affected by chromosomal level.

대학생 흡연자의 금연경험에 따른 건강신념차이

구정일,김민정,김동희,백정희,안현실,은가희,우혜령,이명희,이수현,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

It has been generally known that smoking is one of the worst factor increasing disease and mortality in human health since in 1950's and nowadays. But regardless of various proofs about social or economical problems due to smoking, current smoking rate has not been decreased in our country. Until now, most of tobacco use and its addiction researches have been focused on the adolescent or adult women smokers not on the university students. Therefore, the purpose of this study was to investigate smoking cessation of university smokers and analyze the health belief-perceived sensitivity, severity, benefits and barriers- difference, then to provide basic data for effective smoking cessation strategy for university smokers. The subjects were comprised of 181 university smokers from 15 universities in Seoul. The data were collected by structured questionaires. The questionaires were designed to measure general characteristics, smoking cessation and health belief, based on Ahn(2003)'s result on the Study of Smoking attitude, Behavior and Smoking Cessation of Adult Male and Lee(1987)'s result on Relationships Between the Health Belief Model and Smoking Habits. The data were collected from May 5 to 16. The collected data were analyzed by SPSS program. The actual number, percentiles, means, standard deviation, t-test and ANOVA were done. The result of this study were as follows; 1. The general characteristics of the subjects of this study The range of age was from 18 to 34 and the averaging age was 23. The subjects of this study were 141 males and 40 females. The religions were Christianity 25.4%(46), Buddhism 12.7%(23), Catholic 23.2%(42), no religion 37.0%(67). The residential forms were living with family 59.1%(107), cooking food for oneself 28.7%(52), dormitory 5.0%(9), boarding house 1.7%(3). Smoking starting age were university 43.1 %(78), high school 29.8%(54), middle school 14.9%(27), in the army 6.1 %(11), elementary school 1.1 %(2). 2. The smoking cessation of the subjects 80% of current smokers showed quitting smoking decision and 20% have not tried. The frequency of quitting smoking decision were from 1 to above 8 and mean was 3.06. As for smoking cessation plan, 54.7%(99) current smokers answered yes, 3.9%(7) said no, 35.4%(64) had no idea and 6.1 %(11) didn't answer. As for the reason of never quitting smoking decision, 71.42%(25 among total 35) have no need to quit smoking and the others said difficulty of smoking cessation. As for the main reason of smoking cessation decision, 54.7%(99) answered for the health, 7.2%(13) family advice, 15.5%(28) girl/boy friend's advice, 1.7%(3) to save money. As for the reason to fail smoking cessation, 56.7%(68) were will-lack, 17.5%(21) stress, 15%(18) smoking mood, 10%(12) withdrawl symptom. As for the smoking cessation method, 85.0%(125) were oneself-will, 2.7%(4) way acquired by mass-media, 2%(3) doctor's prescription, 2%(3) religion's help, 0.7%(1) smoking cessation school. As for the difficulty in smoking cessation, 42.2%(62) were drinking meeting, 23.1 %(34) no alternative of stress release, 12.2%(18) withdrawl symptom, 11.6%(17) having nothing to do, 7.5%(11) friend's encouragement. As for the most difficult cigarette to quit, 67.8%(99) were all-day smoking except the first smoking in the morning, 32.2%(47) the first smoking in the morning. 3. Health belief as for general characteristics Gender - The total point in benefits showed higher in male(28.5177) than female(26.6750). There was significant difference(t=.778, p<.05). The total point in health belief showed higher in male (105.8298) than female(l00.5000). So there was significant difference(t=.345, p<.05). Age - There was significant difference in benefits between age(10's-25.83, 20's-28.39, 30's-32.00)(F=3.73, p<.05). Smoking starting age - There was significant difference in total point of sensitivity and health belief(sensitivity F=4.00, p<.Ol, health belief F=2.843, p<.05). As for sensitivity, subjects who started smoking in the army showed the highest(33.36) and ones in high school showed the lowest(26.96). The total point in health belief showed the highest in elementary school(l19.00) and the lowest in high school(100.98). 4. Health belief as for smoking cessation There was significant difference in health belief as for smoking cessation(t=0.189, p=0.OO2). Sensitivity point showed higher in smoking quitting trial smokers(29.2) than non-trial smoker (26.9). Severity point showed higher in trial smokers (30.7) than non-trial smokers(28.9). Benefit point showed higher in trial smokers(28.6) than non-trial smokers(26.2). There was no significant difference in barriers point.

Agrobacterium tumefaciens KU12 로부터 분리한 pTi12 의 Replication Origin 과 벼의 Actin 유전자 프로모터를 이용한 벼의 Binary Vector 제조

박희등(Hee Deung Park),박정구(Jeong Ku Park),이재선(Jae Seon Lee),심웅섭(Woong Seop Sim) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.4

Binary vectors, pBI-ActR1, pBI-ActF1 and pBSH-ActR1, were constructed using pGA642, the replication origin of pTil2 and the rice actin promoter. The sizes of pBI-ActR1, pBI-ActF1 and pBSH-ActR1 were 12.9 kb, 13.2kb and 11.95 kb, respectively. These vectors containing a rice actin promoter followed by a GUS structural gene could induce strongly the expression of GUS gene in transformed rice cells. Rice explants form 3-4 day old seedlings after germination were cocultured with A. tumefaceins harboring pBI-ActR1, pBI-ActF1 or pBSH-ActR1, and then GUS expression in the explants was assayed. Transformation of rice explants by these binary vectors was tissue-specific, such that the meristematic regions of shoot apex, root and hypocotyl were transformed by these binary vectors.

代替에너지 석탄의 개발동향에 대한 考察

鄭求海 忠州大學校 1986 한국교통대학교 논문집 Vol.20 No.1

There has been a rapid rapid progress on the exploitation of substitute engrgy other than petroleum since the oil crisis in 1973. It is estimated that though we seek the technical improvement of oil exploitation with the gradual increase of oil demand, the oil deposits st present will be drained within 30 years. As things go we can suggest the atomic energy, solar energy, hydrogen, geothermal energy or oil plant for our future energy resources though, the most realizable way is to produce the liquefaction or gas fuel from coal. It is because that, assuming that there are total ten thousand billion tons of coal reaerves, we have nearly four times more fuel production than oil converting into 3-4 barrels of fuel production per ton coal. Accordingly we have tried to study on the trend of exploitation of iliquefaction of coal, and also intended to examine the development of oil sand and oil shell as fossil fuel resourcess except co col coal.

고킨와카로쿠죠의 万葉歌 採錄에 관한 연구 : 萬葉集에서의 직접채록의 미증(2)

具廷鎬 단국대학교 단국일본연구학회 1998 일본의언어와문학 Vol.2 No.-

液體燃料 噴舞燃燒에 있어서 NO 生成擧動 硏究

鄭求海,許文會 忠州大學校 1988 한국교통대학교 논문집 Vol.22 No.-

Recently the power engines by combustion are rapidly increased. Accordingly environmental pollutant of exhaust gas from combustion does also increase rapidly. We know that there are NO??, SO??, Co, soot and others in the pollutant and it is strongly requested in society that the Behavior of these pollutant should be elucidated and their discharge should be decreased. For this it is important that the Behavior of environmental pollutants which is generated and become extinct in the burner of practical use should be elucidated. In this point of view I studied on the atomization of liquid fuel which is most utilized and the Behavior of Nox which generates most in that combustion.

토마토 품종간의 오존 감수성 비교

구자형,원동찬,조정희,신대식 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

Seven cultivars of tomato were exposed to 0.15μL/L ozone for 20 hours to determine differences in sensitivity. Based upon the degree of foliar injury, the highest degree of sensitive cultivars were 'Daehyongboksu' and 'Goangsu'. Four cultivars, 'Goangmyong', 'Pungyoung' and 'Pinkglory' including 'Pinkforcer' were separated into intermediate sensitive group. 'Neabyongjangsu' was the least sensitive of the 7 tomato cultivars tested. The degrees of ethylene production and epinasty of leaves were relatively consistent with the differences of sensitivity to ozone. However, stomatal and trichome density, transpiration rate, and activities of peroxidase and superoxide dismutase were not clearly related to the ozone sensitivity of cultivars.

古來風體抄의 만요오歌 : ��제이(俊成)의 選歌意識을 중심으로

具廷鎬 중앙대학교 외국어문학연구소 1997 외국학연구 Vol.- No.1

非鐵金屬 Aℓ- Mg 合金의 時效處理에 따른 强度變化에 關한 硏究

鄭求海 忠州大學校 1978 한국교통대학교 논문집 Vol.11 No.2

In this study, I did not Alloy Cr and Mn in Aℓbut Zn and Mg to prevent Corrosion and natural crack, and studied mechanical properties on aging it from 200℃ to 450℃ after tempering equally it in 450℃ also these Aℓ-Alloys can be used to manufacture Aℓ-chassis, vessels, mechanical instruments and electical instruments.

지각과 지구 내부에 대한 고등학교 학생들의 존재론적 범주화와 정신모형

정구송 韓國敎員大學校 敎育硏究院 2008 敎員敎育 Vol.24 No.2

이 연구는 지각과 지구 내부 영역에 대한 고등학교 학생들의 존재론적 범주화와 정신모형은 분석한 것이다. 연구 대상은 고등학교 2학년 169명 학생으로 연구에 참여한 모든 학생들에게는 정신모형을 밝 히기 위해 그리기 과제가 주어졌고 구조화된 질문안에 따라 존재론적 범주화 경향을 밝히기 위한 면담이 실시되었다. 자료 해석을 위한 그리기 과제물 및 존재론적 범주화의 부호화 틀을 제작하였으며,모든 학생 결과물들은 기준 틀에 따라 각각 4단계로 부호화하였다. 연구 결과에서 69%의 학생들이 정적 정신모형에 해당되는 이해수준 1,2에 부호화 되었으며,존재론적 범주화 경향은 지각과 지구 내부 영역에서 각각 73%, 33%에 해당되는 학생들이 물질과 전환로서 배정하고 있음이 나타났다. 또한 이해 수준과 존재론적 범주화 단계에 따른 교차분석에서 이해수준이 높은 학생일수록 과정 범주로 사고하는 경향을 보였다. The purpose of this study was to investigate students' ontological categories and mental models about the Earth's interior. A total of 169 llth grade students participated in this study. They were required to draw Earth's interior tasks and were interviewed about their ontological categories. A coding frame was designed to classify the students' drawings and interview responses. then four steps of cognitive model and ontological categories was established based on the frame of coding. In the results, The classified mental models showed 69% of all sampled students accounted for the static process. the distribution of ontological categories showed 26%, 8% accounted for matter category about the crust and the Earth's interior. The cross-analysis of these two that the higher level of students' mental model is, the higher level of category analyzed according to study areas.