http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
장명재,조영록,장완성,이애리 경희대학교 체육대학 한국체육과학연구소 2002 體育學論文集 Vol.30 No.-
Necessity of integration education is emphasized by link of disabled persons' right elevation. In the present study, physical director's attitude about integration education was investigated via questionnaire. Seoul and Kyung-gi do area's 424 physical directors participated in this study. Got following conclusion as result that analyze in physical director's individuation and effect of attitude, value, preparation, synthetic aspect. Physical director's attitude about integration education is affirmative comparatively. Distinction of sex, special physical education class taking a course, service experience, disabled student education experience, education satisfaction did not influence in physical director's behavior. This is displaying that much difficulties are in realistic aspect supporting integration education enforcement basically. Teachers who much disabled student education experiences and/or more than graduate school feel bigger burden. These results may become help to find direction to increase professionalism about disabled person integration education forward.
Jei-Wan Lee,Sang-Chul Kim,Sookyung Shin,Ji-Young Ahn,Min-Woo Lee 한국원예학회 2020 원예과학기술지 Vol.38 No.4
This study was conducted to develop microsatellite markers in Koelreuteria paniculata using next-generation sequencing. A total of 71,114,562 reads of 20x coverage for the K. paniculata genome were generated and assembled to 141,924 contigs of a minimum of 500 bp long. One hundred seventy-nine of the 79,633 contigs containing microsatellite regions were used for primer design. Fourteen primer sets were selected as polymorphic markers by applying them to three K. paniculata populations, designated as natural monuments in Korea. Somewhat low levels of genetic diversity were observed. The grand means of the number of alleles, observed heterozygosity, and expected heterozygosity were 2.7, 0.493, and 0.407, respectively. Analysis of molecular variance indicated a high level of genetic differentiation among populations. Distinct patterns of three populations were identified in the principal coordinate analysis and Bayesian structure analysis. The probability of identity of these markers was estimated to be quite low, suggesting that these markers have the robust potential power to distinguish genetically different individuals. K. paniculata has a high economic value as an ornamental tree, and a honey tree. The novel microsatellite markers developed in this study will be useful for future breeding programs and genetic studies aimed at developing conservation plans.
Development of an ISSR-Derived SCAR Marker in Korean Ginseng Cultivars (Panax ginseng C. A. Meyer)
Lee, Jei-Wan,Kim, Young-Chang,Jo, Ick-Hyun,Seo, A-Yeon,Lee, Jeong-Hoon,Kim, Ok-Tae,Hyun, Dong-Yun,Cha, Seon-Woo,Bang, Kyong-Hwan,Cho, Joon-Hyeong The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.1
Recently, new ginseng cultivars having superior agricultural traits have been developed in Korea. For newly developed plant cultivars, the identification of distinctiveness is very important factors not only in plant cultivar management but also in breeding programs. Thus, eighty-five inter simple sequence repeat (ISSR) primers were applied to detect polymorphisms among six major Korean ginseng cultivars and two foreign ginsengs. A total of 197 polymorphic bands with an average 5.8 polymorphic bands and 2.9 banding patterns per assay unit across six Korean ginseng cultivars and foreign ginsengs from 236 amplified ISSR loci with an average 6.9 loci per assay unit were generated by 34 out of 85 ISSR primers. Three species of Panax ginseng including the Korean ginseng cultivars, P. quinquefolius, and P. notoginseng, could be readily discriminated using most tested primers. UBC-821, UBC-868, and UBC-878 generated polymorphic bands among the six Korean ginseng cultivars, and could distinguish them from foreign ginsengs. Sequence characterized amplified region (SCAR) marker system was introduced in order to increase the reproducibility of the polymorphism. One SCAR marker, PgI821C650, was successfully converted from the randomly amplified polymorphism by UBC-821. It showed the expected dominant polymorphism among ginseng samples. In addition, the specific polymorphism for Sunwon was generated by treating Taq I restriction enzyme to polymerase chain reaction products of PgI821C650. These results will serve as useful DNA markers for identification of Korean ginseng, especially Sunwon cultivar, seed management, and molecular breeding program supplemented with marker-assisted selection.
Lee, Min Woo,Seo, Rira,Lee, Yu Jeong,Bae, Ju Hye,Park, Jung-Kwon,Yoon, Joung-Hahn,Lee, Jei Wan,Jung, Ho Won Elsevier 2016 Biochemical and biophysical research communication Vol.480 No.3
<P><B>Abstract</B></P> <P>An <I>Arabidopsis thaliana ALTERED MERISTEM PROGRAM1</I> (<I>AtAMP1</I>), which encodes a putative glutamate carboxypeptidase, not only controls shoot apical meristem development, but also is involved in tolerance response to abiotic stresses. Here, we introduce a novel mutant; named <I>amp1-32</I> that is a phenocopier to previously isolated different <I>amp1</I> mutant alleles. Interestingly, tiny leaves were continuously developed at the bottom of pre-emerged leaves in the <I>amp1-32</I>. The <I>amp1-32</I> mutant was less sensitive to heat shock treatment lasting for 3 h, whereas disease symptoms were severely developed in the mutant after <I>Pseudomonas syringae</I> infection. The mRNA levels of 171 genes were significantly altered in the mutant, as compared to wild-type plants. The transcription of genes involved in hormone signaling, post-embryonic development, and shoot development were up-regulated in the <I>amp1-32</I> mutant, whereas expression of genes related to responsiveness to pathogens and (in)organic matters, were decreased in the mutant. Taken together, perturbation of CK- and ABA-related events by AMP1 mutation caused aberrant development phenotype and conflicting responses against abiotic and biotic stresses in <I>Arabidopsis</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel amp1-32 mutant shows aberrant growth and development phenotypes. </LI> <LI> The amp1-32 mutant tolerates heat treatment, but shows severe symptom development after <I>P. syringae</I> infection. </LI> <LI> Mutation of the AMP1 causes transcriptional reprogramming in Arabidopsis. </LI> </UL> </P>
Development of an ISSR-Derived SCAR Marker in Korean Ginseng Cultivars (Panax ginseng C. A. Meyer)
Jei-Wan Lee,Young-Chang Kim,Ick-Hyun Jo,A-Yeon Seo,Jeong-Hoon Lee,Ok-Tae Kim,Dong-Yun Hyun,Seon-Woo Cha,Kyong Hwan Bang,Joon-Hyeong Cho 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.1
Recently, new ginseng cultivars having superior agricultural traits have been developed in Korea. For newly developed plant cultivars, the identifi cation of distinctiveness is very important factors not only in plant cultivar management but also in breeding programs. Thus, eighty-fi ve inter simple sequence repeat (ISSR) primers were applied to detect polymorphisms among six major Korean ginseng cultivars and two foreign ginsengs. A total of 197 polymorphic bands with an average 5.8 polymorphic bands and 2.9 banding patterns per assay unit across six Korean ginseng cultivars and foreign ginsengs from 236 amplifi ed ISSR loci with an average 6.9 loci per assay unit were generated by 34 out of 85 ISSR primers. Three species of Panax ginseng including the Korean ginseng cultivars, P. quinquefolius, and P. notoginseng, could be readily discriminated using most tested primers. UBC-821, UBC-868, and UBC-878 generated polymorphic bands among the six Korean ginseng cultivars, and could distinguish them from foreign ginsengs. Sequence characterized amplifi ed region (SCAR) marker system was introduced in order to increase the reproducibility of the polymorphism. One SCAR marker, PgI821C650, was successfully converted from the randomly amplifi ed polymorphism by UBC-821. It showed the expected dominant polymorphism among ginseng samples. In addition, the specifi c polymorphism for Sunwon was generated by treating Taq I restriction enzyme to polymerase chain reaction products of PgI821C650. These results will serve as useful DNA markers for identifi cation of Korean ginseng, especially Sunwon cultivar, seed management, and molecular breeding program supplemented with marker-assisted selection.
2분과 : 황벽나무 자연집단의 유전다양성 및 유전구조 분석
이제완 ( Jei Wan Lee ),홍경낙 ( Kyung Nak Hong ),강진택 ( Jin Taek Kang ) 한국임학회 2014 한국산림과학회지 Vol.103 No.1
본 연구는 ISSR 표지자를 이용하여 국내 분포하는 황벽나무 7개 집단의 유전다양성과 유전구조를 분석하였다. 6개의 ISSR primer를 이용하여 분석한 결과 primer 당 평균 4.5개의 다형성 band를 확인하였고, 각집단의 다형성 유전자좌의 비율은 평균 78.8%로 나타났다. Shannon의 유전다양성 지수(I)는 0.421로 나타났고, 이형접합체 기대치(He)는 평균 0.285로 베이즈 방법을 이용한 평균 이형접합체 기대치(hs=0.287)와 유사하였다. AMOVA에서 전체 유전변이의 92.4%가 집단내 개체간 차이에 기인하며, 7.6%는 집단간 차이에 기인하였다. 베이즈 방법을 이용한 유전분화(θII)는 0.066으로 추정되었으며, 전체 집단의 근친교배율(f)은 0.479로 계산되었다. 유연관계 분석과 베이즈 군집분석결과 우리나라 황벽나무 집단은 가평, 화천, 봉평, 용평이 하나의 군집을 형성하였고, 산청 지역의 2개 집단(삼장 및 시천)이 다른 하나의 군집을 형성하였으며, 무주 집단이 산청지역의 집단과 지리적으로 근접함에도 불구하고 독립적인 군집을 나타내었다. Mantel’s test 결과 집단간 유전적 유연관계와 지리적 분포의 상관성은 나타나지 않았다. 황벽나무의 유전자원보존을 위한 대상 집단 선정 시 생태적 및 생활사적 특징과 함께 본 연구결과에서 나타난 유전다양성과 군집구조 분석결과를 고려하는 것이 효과적일 것으로 사료된다. Genetic diversity and genetic structures were estimated in seven natural populations of Phellodendronamurense Rupr in South Korea using ISSR markers. The average of polymorphic loci per primer and the proportion of polymorphic loci per population were 4.5 and 78.8% respectively with total 27 polymorphic loci from 6 ISSR primers. The Shannon`s diversity index(I) was 0.421 and the expected heterozygosity(He)was 0.285, which was similar to the heterozygosity (hs = 0.287) inferred by Bayesian method. In AMOVA,7.6% of total genetic variation in the populations was resulted from the genetic difference among populationsand the other 92.4% was resulted from the difference among individuals within populations. Genetic differentiation(θII) and inbreeding coefficient(f) for total population were estimated to be 0.066 and 0.479 by Bayesian method respectively. In Bayesian clustering analysis, seven populations were assigned into three groups. This result was similar to the results of genetic relationships by UPGMA and PCA. The first groupincluded Hwachoen, Gapyeong, Bongpyeong and Yongpyeong population, and the second included two populations in Sancheong region. Muju population was discretely assigned into the third group in spite of thegeographically short distance from the Sancheong region. There was no significant correlation between genetic relationship and geographic distribution among populations in Mantel`s test. For conservation of thephellodendron trees, it would be effective to consider the findings resulted from this study with ecological traits and life histories of this species.