Possibility of Application of Artificial Insemination Buffer (AIB) for Increasing of Production Efficiency of Female Cow Offspring

Jae‐Il Bang,A‐Na Ha,A‐Ra Cho,Kyeong‐Lim Lee,MD. Fakruzzaman,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

The present research was carried out to evaluate the possibility of female offspring production using artificial insemination buffer (AIB) before artificial insemination (AI). To do it, we carried out the optimization of AIB, making of AIB gun and analysis of affecting AI rate after AIB treatment. AIB made with the base of Tris‐buffer supplemented with L‐arginine and several materials that could be reduced the motility of male sperm compared with female one. This mean that female sperm could be increased the possibility of fertilization with ovum compared with male one. AIB must be deposited into 2nd to 4th cervix by the guide of AIB gun. After 15 min of AIB insertion, frozen semen was deposited into same place after. Total 352 cattle were inseminated with AIB insemination and was not significant difference between AIB and traditional AI rate (56.8 vs. 55.7%). However, AIB AI rate was significantly differs among 12 different farms. The parturition number of cows did not effect on AIB AI rate among 1st to 7th parturition number of cows. The proportion of AIB AI success rates in hanwoo cows was significantly higher than in dairy cows (61.0% vs. 48.7%), but the average AI success rate was not different between AIB and conventional AI (56.8% vs. 55.7%). The female offspring production rate in 2nd to 4th cervix deposition place was significantly higher than in uterus body (77.7% vs. 59.6%, p<0.05). The injection volume of AIB in 5 and 10 ml was significantly higher than in 2 ml (77.7, 78.7 vs. 51.8%, p<0.05), but not different between 5 and 10 ml ABI volume. The best exposure time of AIB in the cervix was 10 and 15 min rather than that of 5 min (79.2%, 77.2% vs. 63.2%, p< 0.05), and so AIB have to expose at least 10 min to get higher female offspring. In conclusion, AIB could be used in AI industry to produce female offspring and also AIB AI can be increased the AI success rate compared with traditional AI rate.

IRAK4 as a Molecular Target in the Amelioration of Innate Immunity–Related Endotoxic Shock and Acute Liver Injury by Chlorogenic Acid

Park, Sun Hong,Baek, Seung-Il,Yun, Jieun,Lee, Seungmin,Yoon, Da Young,Jung, Jae-Kyung,Jung, Sang-Hun,Hwang, Bang Yeon,Hong, Jin Tae,Han, Sang-Bae,Kim, Youngsoo American Association of Immunologists 2015 Journal of Immunology Vol. No.

<P>Mice lacking the IL-1R-associated kinase 4 (IRAK4) are completely resistant to LPS-induced endotoxic disorder or the TLR9 agonist CpG DNA plus D-galactosamine-induced acute liver injury (ALI), whereas wild-type strains succumb. However, translational drugs against sepsis or ALI remain elusive. Lonicerae flos extract is undergoing the clinical trial phase I in LPS-injected healthy human volunteers for sepsis treatment. In the current study, chlorogenic acid (CGA), a major anti-inflammatory constituent of lonicerae flos extract, rescued endotoxic mortality of LPS-intoxicated C57BL/6 mice, as well as ameliorated ALI of LPS/D-galactosamine-challenged C57BL/6 mice. As a mechanism, CGA inhibited various TLR agonist-, IL-1 alpha-, or high-mobility group box-1-stimulated autophosphorylation (activation) of IRAK4 in peritoneal macrophages from C57BL/6 or C3H/HeJ mice via directly affecting the kinase activity of IRAK4, a proximal signal transducer in the MyD88-mediated innate immunity that enhances transcriptional activity of NF-kappa B or AP-1. CGA consequently attenuated protein or mRNA levels of NF-kappa B/AP-1 target genes encoding TNF-alpha, IL-1 alpha, IL-6, and high-mobility group box-1 in vivo under endotoxemia or ALI. Finally, this study suggests IRAK4 as a molecular target of CGA in the treatment of innate immunity-related shock and organ dysfunction following insult of various TLR pathogens from bacteria and viruses.</P>

신장에 발생한 섬유육종 1례

이재규,손경민,권동득,오봉렬,류수방,박양일,최찬 大韓泌尿器科學會 2001 Korean Journal of Urology Vol.42 No.7

SO₂감지를 위한 NASICON 고체전해질 가스센서의 제조 및 특성 평가

배재철,Liu, aiguo,이상태,전희권,방영일,이덕동,허증수 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

30-60대 건강한 서울시민의 脚筋伸展파워 및 신체작업능력 : 동경 및 북경시민과의 비교 Comparison of Tokyo and Peking Residents

서재균,김승일,최영렬,방영진,김형돈,전병관,선우섭,손두옥 한국학교체육학회 1999 한국학교 체육학회지 Vol.9 No.1

본 연구는 한국인의 삶의 질 향상을 위한 국민 생활체육 진흥정책 수립에 필요한 기초자료를 제공하기 위하여, 건강한 30-60대 서울시민(313명)의 체격과 근력, 전신지구력 등의 체력을 측정하여 문화, 경제생활수준이 서로 빠른 동경시민(509명) 및 북경시민(160명)과 비교 검토하는 것을 목적으로 하였다. 각국의 연구대상에 대한 측정은 사전에 협의한 동일한 방법(동경대학 체육과학실법)과 기기를 이용하였다. 체격요인으로는 신장과 체중을, 체력요인으로는 트레드밀 보행시 (90m/분)의 심박수와 보폭 그리고 자전거 에르고미터 운동시의 PWC75%HRmax와 脚筋伸展파워를 측정하였다. 신장 및 체중, 신체질량지수는 남녀 모두 북경, 서울, 동경시민순으로 높게 나타났다. 트레이드밀 보행시의 심박수는 남녀 모두 북경, 동경, 서울시민 순으로 낮은 값을 보였으며, 신장에 대한 보폭비율은 남자의 경우에는 북경, 서울, 동경의 순으로, 여자는 북경, 동경, 서울의 순으로 높았다. 脚筋伸展파워는 60대 여자를 제외한 남녀 모두 북경, 서울, 동경시민의 순으로 높았으며, PWC75%Hrmax는 남자 40대 와 여자 30대를 제외한 모든 연령 대에서 서울시민이 동경시민 보다 높은 경향을 나타냈다. 한편 모든 피검자를 대상으로 연령과 脚筋伸展파워와의 회귀식은 남녀 각각 Y=-0.30X+28.88(n=230, r=0.61, p<.001), Y=-0.l0X +12.49(n=611, r=0.32, p<.001), PWC75%HRmax와의 회귀식은 남녀 각각 Y=-0.01+ 2.50(n=116, r=0.29, p<.01), Y=-0.l0X+1.89(n=461, r=0.20, p<.001)이였다. 따라서 30~60대 서울시민의 체격 및 脚筋伸展파워는 북경시민 보다 낮게 나타나 삶의 질 향상을 위해 보다 적극적인 사회체육 활성화 방안 마련이 요망된다. The purpose of this study was to serve as a basis for making a health promotion policy for better quality of Korean life, by examining 313 citizens of Seoul's physical and leg extension power, physical working capacity who were in their thirties to sixties, enjoying good health, and by making a comparison of tile result with that of 509 citizens of Tokyo and 160 citizens of Peking who lived in different culture and living environment. The findings of this study were as below: First, the male citizens of Peking, who were in their forties to sixties, and the female citizens of the same city, who were in their fifties to sixties, were significantly higher in height, body weight and body mass index. Second, the male citizens of Peking, who were in their thirties and sixties, and the female citizens of the same city, who were in their thirties to forties, were significantly lower in heart rate in walking in the treadmill(90n/min). And there was a tendency that the citizens of Peking showed a higher ratio of stride to height. Third, the male citizens of Peking, who were in their fifties, and the female citizens of the same city, who were in their thirties to forties, had a significantly better leg extension power. As a result of examining all the subjects, there was a negative correlation between leg extension power and age. Fourth, all age groups of Seoul citizens, except males in their forties and females in their thirties, fended to be better in PWC75%HRmax than the citizens of Tokyo. When all the subjects were examined, there was a negative correlation between PWC75%HRmax and age. Consequently, there was a need to prepare more active plan to step up sports fur all successfully in order to improve the quality of life, as the citizens of Seoul who were their thirties to sixties appeared to be inferior in physical physique and leg extension power to the citizens of Peking.

백서 간재생중 H2B Histone 유전자 발현에 대한 Octamer Binding Transcription Factor의 역할

임규,방재웅,최두식,손미영,이영철,박청,김태동,김영래,박종일,윤완희,박승길,황병두 한국노화학회 2001 한국노화학회지 Vol.11 No.1

제조업의 경쟁력과 재무성과와의 인과모형에 관한 실증적 분석

박해철,방재일 中央大學校經營硏究所 2002 經營學論集 Vol.28 No.2

조루증의 치료에서 Paroxetine의 효과와 부작용

박광성,이재규,윤진상,권동득,오봉렬,류수방,박양일 全南大醫大 2001 전남의대학술지 Vol.37 No.1

性別 및 年齡에 따른 體格과 體力의 特性과 變化에 關한 縱斷的 硏究Ⅱ : Emphasised on College Men of Department of Physical Education 體育專功 大學生을 中心으로

金尙國,姜東洹,張周鎬,金昇一,徐再均,金耕知,孫斗玉,鮮于攝,崔榮烈,尹宇相,金亨燉,方永鎭,朴英震,柳在忠 慶熙大學校 體育科學大學 1989 體育科學論叢 Vol.- No.2

The purpose of this study was to compare the characteristics, change in physique and physical function of college students majoring physical education and Taekwondo (454 males and 64 females of age groups ranging from 18 to 26). The results are as following: 1. The male group of 23yrs old in physical education showed highest values in 7 items(Weight, Chest Girth, Fore Arm Girth, Thigh Girth, Calf Girth, Scapular Skinfold Thickness and Suprailiac Skinfold Thickness). 2. In the Physical function, the male group of 18yrs old showed highest values in Power, Flexibility, and then 21yrs old at Back Strength, Vital Capacity, and 23yrs old at Grip Strength, and 25yrs old at Blood Pressure, respectively. 3. In the physique, the male group of 22yrs old showed highest values in Weight, Chest Girth, Thigh Girth, Calf Girth, and then 24yrs old Upper Arm Girth, Fore Arm Girth, and 19yrs old at Height, Upper Limb Length, respectively. 4. In Physical Function, the 22yrs old showed highest values in Back Strength, Vital Capacity, and then 23yrs old at Side Step, Trunk Extension, but 18yrs old showed lower Values in Grip Strength, Side Step, Vital Capacity and Blood Pressure. 5. The female group of 22yrs old showed highest values in 8 items(Height, Sitting Height, Waist Girth, Upper Limb Length, Lower Limb Length, Skinfold Thickness), and then 18yrs old at 5 items (Weight, Upper Arm Girth, Fore Arm Girth, Thigh Girth, Calf Girth), respectively. 6. In the physical function, the 22yrs old showed highest Values in Grip strength, Back Strength, Power, 5 Minute Run, and 18yrs old at flexibility, Harvard Step Test, respectively. 7. In the change of the physique for two years(1988-1989) in the male group, majoring Physical education the 22yrs old showed increase in 4 items, and the 20yrs old in 3 items, but 18yrs old showed decrease in 3 items. 8. In the change of the physical function for two years in the male group, the 20yrs old showed increase in 4 items, and the 21, 25, 26yrs old in 3 items, but the 19yrs old showed decrease in 6 items, and 18, 22 yrs old in 5 items, respectively. 9. The change of the physique for two years in all female group, of 18, 19, 20, 21yrs old showed increase in 11 items except Skinfold Thickness. 10. In the change of physical function for two years in female group, the 18yrs old showed increase in 3 items, and then 20yrs old in 5 items, but 19, 22yrs old decreased 12 items, respectively. 11. The highest significant difference in physique between male and female was found in weight(mean Value 17.5%), and the lowest difference was found Thigh Girth, Calf Girth(1,3%), Upper Limb Length, Lower Limb Length(2.5%). 12. The Highest difference in physical function was Strength (mean Value 40%), Vital Capacity (33%), Power(22%), but Lower Trunk Flexion, Foot Balance(3.4%). 13. In the difference of Physique and physical function between physical education major and taekwondo major, the physical education showed higher values in skinfold Thickines (mean Value 16.5%), Weight, Waist Girth(4.7%) and Strength(33%), Endurance(5.6%), but subjects majoring Taekwondo showed higher values in Flexibility and Foot Balance. 14. In all male groups, Waist Girth, Scapular Skinfold Thickness, Back Strength, Sargent Jump, Trunk Extension and Diastolic Blood Pressure were showed a significantly related at 0.001 level.

性別 및 年齡에 따른 體格과 體力의 特性과 變化에 關한 縱斷的 硏究 Ⅰ : 體育專攻 大學生을 中心으로 Emphasised on College Men of Physical Education

金尙國,姜東洹,金昇一,徐再均,金耕知,孫斗玉,鮮于攝,崔榮烈,尹宇相,金亨燉,方永鎭,李錫元 慶熙大學校 體育科學大學 1988 體育科學論叢 Vol.- No.1

The purpose of this study was to compare the characterists and change in physique and physical function by college student of physical education major(287males and 40 females of age groups ranging from 18 to 26). The results were as following; 1) The characteristics of physique and physical function by sex and age. ① The male group of 18yrs old showed higher values in Height, Weight, Upper Limb Length, Strength, Flexibility, and Vital Capacity than any other groups, but female group showed lower values in Height, Weight, Chest Girth, Lower Limb Length, Thigh, Girth, Calf Girth and all of the Physical function. ② The male group of 19yrs old showed high values in power and Agility, then female group showed in Strength and Flexibility. ③ The male group of 20yrs old showed the lower values in Weight, Waist Girth, Upper Limb Length, Fore Arm Girth, Thigh Girh.Calf Girth, Vital Capacity, and female group are very lowed in Upper Arm Lenght, Fore Arm Length, Skin Fold Thickness and 5 minute Run. ④ The male group of 23yrs old showed the high values in Upper Arm Girth, Fore Arm Girth and Balance, and then female group higher in Weight, Chest Girth, Waist Girth, Upper Arm Girth, Fore Arm Girth, Thigh Girth, Power, Shuttle Run, Balance and vital capacity than any other age Groups. ⑤ In male group, Chest Girth, and Blood Pressure were significantly related, and Side Step and Trunk Extension were nagatively related to the age at 0.1% level, and then in female group, Sargent Jump, Standing Long Jump and Vital Capacity were showed a significantly related at 0.5% level. 2) The difference of physique and physical function between male and female by age. ① Everage difference in whole physique items except skinfold thickness was 9.8%, and that 21.1% in all of physical function except Trunk Flextion between male and female group subjects. ② About the ages, the 18yrs old male group showed the 22.8% higher values of 26 items, but 22yrs old male group were about 12.2% in 23 items, thus it is very lower than any other groups. 3) The difference of physique and physical function between General group and physical education group in age-matched. ① In the physique male physical education groups showed about 7% higher in Weight, Chest Girth, Upper Limb Length, Upper Arm Girth, Thigh Girth, and female group showed about 7% higher in Weight and Upper Limb Length. ② In the physical function, Trunk Flextion, 5 minute Run, and Blood Pressure(Diastolic) were about 8% high values in whole group, and female group showed about 8% higher in Standing Long Jump, Shuttle Run, and Trunk Extension. ③ In 22yrs old male and female physical education group were very dominated in physique and physical function than the other age group against the general group.