결핵성 수막염 환자의 뇌척수액에서 결핵균 30-kDa항원에 대한 항체반응

백태현,박재하,김화중,조은경,최대경 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.1

Rapid diagnostic tests for tuberculous meningitis are urgently needed because delayed treatment increase the already high mortality rate of this disease. In this study, the 30-kDa protein antigen that purified from the unheated culture filtrate of M. tuberculosis H_37Rv was examined for its diagnostic usefulness in detecting mycobacterial antibody in cerebrospinal fluid(CSF) by the enzymelinked immunosorbent assay(ELISA) and Western blot. The mean ELISA values of IgG antibody activity to 30-kDa antigen in CSF of 12 patients with tuberculous meningitis and 17 non tuberculous patients were 0.479±0.245 and 0.051±0.024, and for the IgM antibody, were 0.375±0.223 and 0.065±0.039, respectibely. The sensitivity and specificity were 100% and 94% for IgG antibody, and 75% and 94% for IgM antibody, respectively. Western blot analysis was the same as that results of ELISA. In the positive determination, however, Western blot could be easily determined by the demonstration of distinct band of 30-kDa molecule. From the above results, it is suggested that the 30-kDa antigen is useful for the diagnosis of tuberculous meningitis, and Western blot shows promise as a useful immunological method for the diagnosis.

우울증환자에서의 Dexamethasone억제검사에 의한 비억제군과 억제군의 비교

백인호,김은혜,김재현 大韓神經精神醫學會 1987 신경정신의학 Vol.26 No.3

Dexamethasone suppression test (DST) was performed on inpatients of Kangnam St. Mary's Hospital, Catholic University Medical College from March 1, 1983 to May 31, 1986 and 13 DST non-suppressors and 17 DST suppressors were chosen optionally from those diagnosed as major depression. DST non-suppressors and suppressors were compared of their clinical features, differences in the diagnostic criteria and treatment outcome. Eighteen clinical features were selected from Diagnostic and Statistical Manual of Mental Disorders 3rd ed. (DSM-Ⅲ), Research Diagnostic Criteria (RDC), Newcastle Endogenous Depression Diagnostic Index (NEDDI), Michigan Discriminant Index (MDI) and Hamilton Endogenomorphic Subscale (HES), which are diagnostic criterias of endogenous depression. Relative correlations of above 5 diagnostic criteria and DST non-suppressor were compared. GAS score and duration of hospital stay were used to compare treatment outcome of non-suppressor and suppressor groups. 1) Among the 18 clinical features, no specific clinical feature was related to DST non-suppressors. 2) The relationships between DST non-suppressors and the diagnosis of endogenous depression by each 5 diagnostic criteria DSM-Ⅲ, RDC, NEDDI, MDI, HES were compared. No single diagnostic criteria of endogenous depression could be said to be related with DST non-suppressors. 3) The abilities of DST in predicting the severity of depression and treatment outcome were evaluated with Global Assessment Scale and duration of hospital stay but was not statistically significant.

결핵균 30 kDa 단백항원 자극에 의하여 유도되는 IFN-Y 및 IL-4 mRNA 발현 양상과 세포독성능

백태현,조은경,박재하,박정규,김화중 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

30 kDa protein antigen, a major secreted protein antigen of Mycobacterium tuberculosis, exhibits strong T cell stimulatory activity. In order to better understand the immunologic activities of 30 kDa antigen, lymphocyte proliferation by ^3H-thymidine incorporation, cytokine mRNA expression pattern using reverse transcription-polymerase chain reaction (RT-PCR), and cytotoxicity in response to in vitro stimulation of human peripheral blood mononuclear cells (PBMCs) with 30 kDa antigen were evaluated. Lymphoproliferative responses to 30 kDa and crude protein antigens in PBMCs of normal PPD-negative and positive donors were compared. As expected, PPD negative donors demonstrated no thymidine incorporation in response to 30 kDa and crude protein antigens, while PPD positive donors showed extensive proliferation to both antigens. Freshly isolated PBMCs were stimulated with 30 kDa antigen for 0, 6, 12, 24, 48 hr, 1 wk, and 2 wk and examined for the induction of IFN-γ and IL-4 mRNA using RT-PCR. The expression of IFN-γ mRNA was greatly augmented after 1 wk, and there was also early inductions at 6 and 24 hr, whereas IL-4 mRNA is consistently expressed through 0 to 48 hr and markedly decreased after 1 wk. In contrast, freshly isolated human tonsillar cells failed to express detectable level of IFN-γ mRNA but showed enhanced IL-4 mRNA expression after in vitro stimulation with 30 kDa antigen for 1 wk. Both natural killer (NK) and T cell cytotoxic activities induced by 30 kDa and crude antigens were also evaluated. PBMCs from PPD positive donors stimulated with 30 kDa antigen showed significantly higher NK and T cell cytotoxic activities than those in PPD negative donors. Crude antigen also induced similar level of cytotoxicity with that of 30 kDa antigen. These results suggest that 30 kDa antigen of M. tuberculosis may selectively activate Th1 cells and be a strong inducer of IFN-γ expression. In conclusion, 30 kDa antigen can be used as a standard T cell immunogen.

여성장애근로자의 특성과 임금 결정요인

이재서,백은령 한국장애인고용촉진공단 고용개발원 2008 장애와 고용 Vol.18 No.1

본 연구는 여성장애근로자의 특성을 파악하고 임금 결정요인을 규명하는데 목적이 있다. 이를 위해 2005년도 장애인근로자실태조사를 활용하여 이차자료분석을 하였다. 분석대상은 상시근로자 5인 이상 사업체에 근무하고 있는 여성장애근로자 2,187명이며 분석을 위해 빈도분석과 다중회귀분석을 실시하였다. 먼저 빈도분석을 통해 여성장애근로자의 일반적 특성과 근로관련 특성을 파악하였다. 다음으로 다중회귀 분석결과, 장애요인으로는 장애로 인한 작업불편여부가, 인적자본요인 중에서는 나이, 학력, 월 근무시간, 현재 직장 취업 경로, 근속연수가, 노동시장요인으로는 계약형태, 고용상태, 사업체규모가 여성장애근로자의 임금에 통계적으로 유의미한 영향을 미치는 요인인 것으로 분석되었다. 이러한 연구 결과를 바탕으로 여성장애근로자의 근로기회 확대와 임금 향상을 위한 방안을 검토하였다. The purpose of this study is to examine the current situation of the women workers with disabilities in korea and identify factors that affect their wages. For this purpose, ‘2005 Survey on the Workers with Disabilities’was used. The subjects for the study are composed of 2,187 fulltime women workers with disabilities at firm size more than 5 persons. Using frequency analysis. the current situation of the women workers with disabilities was reviewed to general characteristics and working condition. and then using multiple regression analysis. wages was analyzed with disability factors, human capital factors, labor market factors. Among these factors, variables of inconvenience with disability for working, age, education, monthly working hours, course of employment, the length of service, contract type, employment status, firm size have statistically significant effect on the wages. Finally, based on these results several policy implications were presented.

광조형 시스템의 리코팅 공정 개선

이은덕,심재형,안규환,백인환 한국공작기계학회 2003 한국생산제조학회지 Vol.12 No.1

Keeping the layer thickness constant is very essential for improving the shape accuracy in the stereolithography process. The layer thickness is created by recoating process, and also affected by recoating parameters such as blade speed and thickness. The created layer in this process can determine the whole accuracy of the entire parts. The aim of this paper is to improve the accuracy of the layer thickness by adjusting the recoating process parameters. Several experiments with different recoating conditions are performed to find the optimal recoating parameters that produce the most accurate layer thickness. The effective recoating method is suggested by measuring and analyzing the cured layer thickness.

초등학생들의 열과 온도에 대한 대안개념 조사

최행숙,김은경,백성혜,이길재,정완호 한국교원대학교 과학교육연구소 2001 청람과학교육연구논총 Vol.11 No.1

In this study, the contents of elementary school science textbooks related to 'heat and temperature' are analyzed and alternative conceptions of "heat and temperature" among students enrolled in grades 4, 5 and 6 are investigated. 259 students were selected from a elementary school located in urban area. The relationships between students' alternative conceptions and the content of elementary science textbooks are also examined. Students' conceptions are analyzed from their answers to a paper-and-pencil test. The typical alternative conception of "heat and temperature" held by students was follows. They think that "heat" is a material and "temperature" is value of heat amount. They can't distinguish between "heat" and "temperature". Their explanation of "heat and temperature" is focused on hot or cool sensation and other observable characteristics of a material. A textbook analysis indicated that contents on "heat and temperature" were organized without the viewpoint of particle motion theory. This may be one of the causes of students' alternative conceptions.

결핵균 30 kDa 항원과 Triton X-100 Solubilized Protein 항원에 의한 대장암 주변 림프절 단핵구의 활성화

박정규,김광호,조은경,임재현,민들레,송영자,김화중,백태현 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Tumor-draining lymph node mononuclear (TDLMN) cells are specifically sensitized to the growing tumor but such cells are deficient for mediating an antitumor response. In this study, we examined the feasibility of using mycobacterial 30 kDa or Triton X-100 solubilized protein (TSP) antigen to stimulate mononuclear cells of colon cancer-draining lymph node for the generation of cell mediated immune effector cells. The proliferative response of TDLMN cells stimulated with mycobacterial 30 kDa or TSP antigen was determined by ^(3)H-thymidine incorporation assay. The proliferation of TDLMN cells to mycobacterial 30 kDa or TSP antigen was significantly increased in PPD (+) patients, but a poor response to the 30 kDa or TSP antigen was observed in PPD (-). The expression on γδ T cells to mycobacterial 30 kDa or TSP antigen was assessed by flow cytometry. The γδ T cells from PPD ( + ) patient responded only to 30 kDa antigen but to TSP antigen. An investigation of cytokine mRNA expression was undertaken using reverse transcription-polymerase chain reaction (RT-PCR) to follow TDLMN cells stimulated with the 30 kDa or TSP antigens for 5 days. The IFN-γ and TNF-α mRNA expression was only induced in TDLMN cells of PPD ( + ) patient in response to the 30 kDa or TSP antigen. The IL-2 mRNA expression was induced in both PPD (+) and PPD (-) in response to the 30 kDa or TSP antigen. But the IL-4 mRNA expression was not induced in response to the 30 kDa or TSP antigen. These results suggest that the 30 kDa and TSP antigens may serve as biologic response modifier for the generation of cell mediated immune effector cells.

식품영양분야의 심화교육 수요조사 : 부천 및 인천 지역을 중심으로 Focusing on Bucheon and Inchein Area

소명환,조신호,변기원,백재은,이수정 부천대학 2002 論文集 Vol.23 No.-

식품영양분야의 심화교육을 실시하기 위하여 부천 및 인천지역의 식품관련 업체에 근무하는 사람을 대상으로 교육수요에 대한 설문 조사를 실시하였다. 설문조사에 성실하게 응답한 126명중에서 전공심화교육을 긍정적으로 평가하고 기회가 되면 교육을 받겠다고 응답한 사람이 101명(80.2%)이었고, 부정적으로 평가하거나 심화교육을 받을 의사가 없다고 응답한 사람이 25명(19.8%)이었다 심화교육을 희망하는 주된 이유는 전문분야의 부족한 실력을 향상시키고 학점은행제에 의하여 학사학위도 받을 수 있기 때문이었다. 전공심화과정에 개설되었으면 좋겠다고 생각하는 전공과목은 영양상담, HACCP실무론, 식생활과건강, 외식산업창업및경영, 식당경영론, 급식전산실무, 식품및조리원리, 식품재료학, 식품관능검사, 식품품질관리 등이었다. 학정은행제에 교양관련과목으로 개설되면 좋겠다고 생각하는 과목은 PC활용, 재태크론, 전자상거래론, 소자본창업경영, 대인관계심리학, 상담심리학, 운동과 건강 등이었다. The aim of research was to investigate the demand for deepened education of the subjects who work in food related jobs in Bucheon and Incheon area, and to establish the deepened education in field of Food and Nutrition. Subjects were 126 persons, and the results were as follows: 101 persons(80.2%) evaluated positively the deepened education, and answered to be willing to take the course if they have an opportunity for study. 25 persons (19.8%) responsed negatively, or answered not to take the course. The main reasons to select the deepened education are not only to be able to improve the knowledge in major but also to obtain the degree of Bachelor in food and nutrition. The courses they wanted to be opened were as follows: nutrition education, practice in HACCP, food and health, restaurant management, practice in computer related to food service, food science, principles of cookery, food materials, sensory evaluation of food, quality control, etc. The general courses they wanted to be opened were as follows: PC practice, theory of property management, electronic commercial transaction, establishment and management of the small sized capital enterprises, psychology in human relation, psychology in counseling, physical activity and health, etc.

Tteokguk, Rice Cake Soup

Paik Jae-eun Professor of Food and Nutrition, Bucheon College The Korea Foundation 2007 Koreana (English) Vol.21 No.4

Rice Porridge (Juk): A Practical Source of Nutrition

Paik Jae-eun Professor of Food and Nutrition, Bucheon College The Korea Foundation 2008 Koreana (English) Vol.22 No.1