Dong-Chun Lecture : Cell-based High Content Screening (HCS) for action modes of drug targets by using systematic deletion mutants of Schizosaccharomyces pombe

( Dong Uk Kim ),( Im Seon Woo ),( Mi Sun Won ),( Young Joo Jang ),( Kyung Sook Chung ),( Han Oh Park ),( Hyang Sook Yoo ),( Kwang Lae Hoe ),( Doo Sik Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

트랜스글루타미네이스(Transglutaminase, TGase)의 기능성과 수산식품에의 응용

주동식,조순영 강릉대학교동해안지역연구소 1998 東海岸硏究 Vol.9 No.1

Mammalian transglutaminase (TGase) and microbial transglutaminase(MTGase)were characterized in aspects of the application in food science. In addition, the effects of TGase or MTGase on the texturization of fish surimi gels, the desalted processing of Kamaboko, and the processing for uniting many of small size fish fillets to a large size one were described. Because of the produced amount the MTGase was more comfortable than TGase extrated from the mammalian liver tissue in aspects of application in seafood science. The Kamaboko gel prepared in the presence of TGase additive was evidently different from that product without the additive. The gelling property of the frozen surimis was clearly strengthened by the additive containing TGase. However, the quality of Kamaboko gel produced from a low grade surimi (such as 2nd grade, C) by applying the additive containing TGase was not comparable to that from a high grade frozen surimi, such as SA without applying the same additive as above. TGase adding was very effective in uniting the small size fish fillets to a large size one and producing comparably the low content salt added fish product.

合成洗劑 生分解 및 環境毒性에 관한 硏究

朱興珪,崔圭行,吳東植 조선대학교 약학연구소 1990 藥學硏究誌 Vol.12 No.1

Alkyl benzene sulfonate, Persists for long Periods in stream because of its resistance to biologic degradation. Its biodegradation is varied in the environment. This investigation was undertaken in oder to know the biodegradation of synthetic detergents which are comprising soft and hard form from may to september 1990. The biodegradation by settling, aeration and activated sludge were determined. The reduction rates of ABS were 22.92%, 10.05% by settlings for 5 days at 25℃ and 52.20%, 82.74% by aeration and average rate 90.98% of LAS by the activated sludge processing. When activated sludge Processing is carried out in the 30% of LAS, the significant downfall of the biodegradation rate to 87.59% is caused by the withering up of bacteria activity as the poison of LAS directly affects microorganism. In dealing with the TLm of LAS systemed synthetic detergent during 24 hours has revealed 40 ppm and the TLm during 48 hours has revealed 24 ppm.

지역특산농수산물의 가공과 상품화

주동식,조순영 강릉대학교동해안지역연구소 1999 東海岸硏究 Vol.10 No.2

Agricultural affairs and fisheries are important to keep a constant income for residents living in a local region. In advance, they play a role in keeping a clean environment of the local region. Recently, it is concluded that selling many special regional products is the most important business in a tourism local. That is to say, to improve the earning income in a tourism local such as a local town, selling the commercial products processed from the principal bioresources in a local region at a high price is important. Therefore, farmers and fishers living currently in a local region should learn new processing technologies and economic circulation technics.

미세조류의 유용성 및 해산 남조류의 생리활성물질

주동식,조순영 강릉대학교동해안지역연구소 1999 東海岸硏究 Vol.9 No.2

The microalgae are a large and diverse group of photosynthetic microoranisms spanning seven division and comprising several thousand species. Of this large number of species, only a few have been studies in some detail with respect to physiology, biochemistry, and potential for mass culture are known for only a few of these. The mass cultivation of a few microalgae has been investigated mainly from the standpoints of food and feed production, recycling of industrial and agricultural waste, treatment of waste-water and the supply of raw material for certain natural compounds. For these purposes, the desirable properties of algae used for mass production-high growth rate, resistance to environmental fluctuations, non-toxic, high nutritive value, high protein content, easy to harvest and to process, good digestibility, resistance to contaminations and ability to grow on sewage. In this review, the utilities of a few microalgae(Chlorella, Spirulina, Porphyridium and Dunaliella) and biological activity materials of seawater microalgae were described.

Bacillus sp. LJ-25가 생성하는 extracellular chitinase의 정제 및 특성

주동식,조덕제,이정석,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

The extracellular chitinase from Bacillus sp. LJ-25 was pruified Sephadex G-100 gel filtration, DEAE-Cellulose and CM-Spephadex C25 column chromatography. The pruified chitinase showed a single band on disc and SDS polyacrylamide gel electrophoresis and the molecular weight was estimated to be about 50KDa. The Optimum pH and temperature for the activity of the purified extracellular chitinase were 7.5 and 35℃, respectively. The activity of chitinase was strongly inhibited by metal ions such as Zn²+, Ba²+, Co²+, Mn²+ and Cu²+. The purified chitinase did not hydrolyze N,N??-diacetylchitobiose, N,N, N'-triacetylchitotriose and p-nitrophenol-N-acetyl-β-D-glucosaminide, which are known to be the substrates of exo-type chitinase. The products in hydolysis of colloidal chitin with the chitinase were N-acetylglucosamine, N,N'-diacetylchitobiose and N,N,N'-triacetylchitotriose.

미역 양갱의 제조 조건

주동식,조순영 강릉대학교동해안지역연구소 1998 東海岸硏究 Vol.9 No.1

미역의 이용성 증대를 위한 일련의 연구로 미역양갱의 제조를 시도하여 제조조건을 구명하였다. 옥수수 전분을 겔화 매체로 이용하여 미역 양갱을 제조할 경우 미역과 전분의 배합비는 1:3의 비율이 적절했고, 설탕 첨가농도는 전체중량의 35 %, 물엿의 첨가농도는 5 % 가 적절하였다. 겔화에 영향할 수 있는 물의 pH는 중성부근일 경우 겔화에 별다른 영향을 미치지 않았다. 또 메밀을 겔화 매체로 이용할 경우도 미역과 메밀의 배합비는 1:3 이 적합하였고, 설탕 및 물엿의 첨가량은 전체중량의 30%, 5%였다. 두 제품 모두 첨가되는 설탕 및 물엿이 양갱의 물성에 크게 영향을 주는 것으로 나타났다. 가수량은 가열시간과 상관성을 가지는데 가수량이 많을수록 가열시간이 길어졌고, 가수량이 적으면 최종제품의 성상이 양호하지 못하였는데, 이러한 점을 고려하여 가수량과 가열시간을 결정한 결과 가수량 및 가열시간은 각각 300-350㎖ 및 35-40분 이었다. Conditions of yankeng processing with sea mustard, corn starch or buckwheat were examined for the effective utilization of sea mustard as a food source. Mixing ratio of sea mustard and corn starch-gelling ingredient-was 1:3, and additive concentration of sugar and starch syrup were 35% and 5% to total weight. In case of buckwheat using as gelling ingredient, mixing ratio of sea mustard and buckwheat was also 1:3, and optimal concentration of sugar and starch syrup were 30% and 5% to total weight. Additive volume of water and heating time were 300-350㎖ and 35-40min in two case.

SCM을 위한 ATP 모델링에 관한 연구

진동주,양광모,박주식,강경식 明知大學校 産業技術硏究所 2002 産業技術硏究所論文集 Vol.21 No.-

최근 기업활동의 자재구매, 제조, 보관 및 운송, 유통/판매까지 포함한 공급체인 전체에 대해 통합관리가 중요한 과제로 부각되고 있다. 현대의 고객들은 제품이나 납기, 품질과 같은 부문에서 높은 서비스를 받고 싶어한다. 이에 따라 오늘 날의 대부분의 기업들은 고도의 기술을 요하는 정보시스템의 도입과, POS, Data Mining 등과 같은 기술들을 이용하고 고객의 요구사항들을 적시, 적소에서 파악하여 고객이 요구하는 상품과 서비스를 공급하기 위하여 최선의 노력을 다하고 있다. 최초의 공급자로부터 소비자까지 상품이 전달되는 과정 중에서 재고와 물류기용은 모든 비용의 대부분을 차지하고 있다. 이에 대한 비용을 줄이려고 하는 것이 공급체인관리(SCM)의 기본적인 목적이라 하겠다. 이에 본 논문에서는 공급체인관리(SCM)에 대한 개념과 배경, 필요성 등을 정리하고 주요 SCM solution에서 제공하는 기능 및 사용된 방법론을 분석하여 이를 바탕으로 주문 접수 시 주문물량의 가능납기를 산정하기 위한 입력데이터, 고려요소, 연산절차 등을 포함한 ATP(Available to Promise) 모델을 제시한다.

족저압력분포 측정장비를 이용한 골프 스윙시 족저압 분석

이동기,이중숙,이범진,이훈식,김용재,박승범,주종필 한국운동역학회 2005 한국운동역학회지 Vol.15 No.1

D. K. LEE, J. S. LEE, B. J. LEE, H. S. LEE, Y. J. KIM, S. B. PARK, J. P. JOO. PIantar foot pressure analysis during golf swing motion using plantar foot pressure measurement system. Korean Journal of Sport Biomechanics, Vol. 15, No. 1, pp. 75-89, 2005. In this study, weight carrying pattern analysis and comparison method of four foot region were suggested. We used three types of club(driver, iron7, pitching wedge). This analysis method can compare between top class golfer and beginner. And the comparison data can be used to correct the swing pose of trainee. If motion analysis system, which can measure the swing speed and instantaneous acceleration at the point of hitting a ball, is combined with this plantar foot force analysis method, new design development of golf shoes to increase comfort and ball flight distance will be available. 1. Address acting, forces concentrated in rare foot regions and lateral foot of right foot. Back swing top acting, relatively high force occurred in medial forefoot region of left foot and forefoot region of right foot. Impact acting, high force value observed in the lateral rarefoot region of left foot and medial forefoot region of right foot. Finish acting, force concentration observed on the lateral region and rarefoot region of left foot. 2. Forces were increased in address of right foot with clubs length increased. All clubs, back swing top acting, high force value observed in the lateral forefoot region of right foot. All clubs, in impact, high force value observed in the lateral rarefoot region of left foot and medial forefoot region of right foot. Finish acting, force concentration observed on the rarefoot region in driver and lateral foot region in iron on left foot. 3. Right foot forces distribution were increased in address, back swing top and left foot force distribution were increased in impact, finnish

머리염색이 인체에 미치는 영향

윤형식,황성호,이현륭,김수호,박연석,권낙현,정호진,김동훈,노현주,홍성호,박병찬,이관,정해관 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

일상생활에서 모발염색은 흔히 접할 수 있는 미용의 한 종류로 특히 젊은층을 중심으로 폭발적으로 유행하고 있다. 염색을 위항 사용하는 약제는 표백제와 발색제 등 각종 화학약품이 사용되고 있으나 이로 인한 건강장해에 대한 연구는 그리 많지 않다. 저자들은 염색이 인체의 모발건강에 미치는 영향을 파악하기 위하여 염색과 관련된 주관적 증상과 모발의 변화에 대한 실험적 연구를 시행하였다. 동국대학교 경주 캠퍼스 재학생 80명을 대상으로 설문조사를 시행하여 염색 유 ·무 및 염색 후에 경험한 증상에 대해 설문 조사하였고, 의과대학 재학생 46명을 대상으로 피부 반응 테스트를 실시하였다. 또한 염색 전후의 모발 탄성도를 측정하였고 모발의 상태를 파악하기 위해 전자 현미경검사를 실시하였다. 설문조사 결과 염색 전에 비하여 염색 후 안구혼탁, 안구건조, 시력저하, 발진 및 접촉성 피부염, 모발손상, 모근손상 등의 증상을 더 많이 경험한다고 호소하였다(p<0.05). 모발손상과 모근손상은 헤어드라이어 사용 빈도에 따라 증가하는 것으로 조사되었다(p<0.05). 피부반응검사에서 가려움증이 가장 많은 증상이었으며 이는 여성보다는 남성에서 높은 것으로 조사되었다. 염색 전후의 모발장력은 염색 전 134.5±10.37g, 염색 128.0±30.69g, 염색 이틀 후 112.5±19.69g으로 나타났다. 염색 전후의 모발의 전자현미경 케라틴 층이 현저히 감소하고 모발이 가늘어지는 차이를 보였다. 염색은 모발손상, 모발 케라틴 손상 및 모근 손상, 발진 및 접촉성 피부반응, 안구혼탁, 안구건조, 시력 저하를 유발한다. 따라서 염색약으로 인한 손상에 대한 주의와 예방이 필요하다고 생각한다. 예방대책으로 염색 전 피부테스트를 통한 적합성 여부를 판단하는 것이 필요하며 가급적 염색을 피하는 것이 좋을 것이다. 염색약에 발암물질이 포함되어있다는 보고도 있어 염색 제조사의 철저한 실험과 염색 물질의 선별이 염색으로 인한 부작용을 최소화하는데 중요한 역할을 할 것이다. Hair coloring has became one of the most popular cosmetic activities to younger generations during last decade. However, there are few studies on the health effect of widespread use of chemical dyes. This study was conducted to study the effects of hair coloring dye on hair and other systems. We conducted a questionnaire survey of 80 persons in Kyongju campus, Dongguk University. We have done open patch skin test on 46 medical students. We also conducted scanning electron microscopy to examine the hair strength and structure before and after hair coloring process. Injury of hair and hair bulb, contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity were the main symptoms complained after hair coloring (p<0.05). Injury of hair and hair bulb were increased by frequency of hair-dryer use(p<0.05). In open patch test, pruritus was complanined by more than half of the subjects. Mean strength of hairs before and after hair coloring was as follows; 134.5 (SD 10.37)g before hair coloring, 128.0 (SD 30.69)g immediately after hair coloring, and 112.5 (SD 19.69)g after two days. The scanning electron microscopic findings of hair surface before and after hair coloring showed decreased keratin layer and thinning of the hair. Hair coloring induces injury to hair, its keratin layer, and hair bulb as well as contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity. Therefore, we think that precaution is needed in use of hair coloring dye. To prevent complications induced by hair coloring dye, it is necessary, especially to those with allergy or skin disorders, to perform skin test before action and avoid hair coloring whenever possible. Longterm health effects of hairdye should be studied and manufacturing companies should try to minimize complications induced by hair coloring dye.