http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
오정석,함인혜,김형민,최호영 대한본초학회 2005 大韓本草學會誌 Vol.20 No.1
Objectives : This study was to investigate the anti-immobility effect of Cistanche deserticolae Herba (CD) and Cistanche salsae Herba (CS) Methods : Forced swimming test (FST) and changes of blood biochemical parameters related to fatigue, glucose (Glc), blood urea nitrogen (BUN), latic dehydrogenase (LDH) aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total protein (TP) were investigated. Results : CD and CS were orally administered to mice for 7 days. After 7 days, the immobility time was significantly decreased in the CD-treated group and CS-treated group in comparison with the control group. The contents of serum AST were significantly decreased and contents of Glc and LDH were decreased in CD-administration group . However, it had no effect on the elevation of TP level, whereas, the contents of GIc were increased and the contents of ALT, BUN and LDH in the blood serum were decreased in the CS (0.01 g/kg/day)-fed group. Conclusions : These results suggest that Cistanche herba, CD and CS may have immune-enhancing effect.
Inhye Jeong,Wen Yan Huang,Sang Hoon Lee,Seong-Ju Oh,Laura Amaya Quiroz,Young-Shick Hong,Bok Kyung Han,Young Jun Kim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Immature sword bean pod (ISBP) has long been known to be effective against bronchial diseases and inflammation. Although research on the mechanism of its efficacy is ongoing, research on plasma metabolites has not been conducted. In this study, the efficacy and metabolite analysis of hot water extract of ISBP were determined in OVA-induced mouse model. The levels of histamine and IgE increased by OVA induction were decreased in a dose-dependent manner by ISBP treatment. Histological analysis confirmed by H&E staining showed that the lung tissue damaged by OVA induction was recovered by ISBP treatment. Compared to the control group, the level of creatine was decreased and that of citrate was increased in the OVA group which indicated creatine may cause dysregulation in blood glucose and inflammation. Moreover, acidification by citrate had progressed and the increased lactate level observed in the dexamethasone and low ISBP groups was assumed to be related to reduced inflammation. Moreover, total lipid level was decreased with ISBP groups. In conclusion, this study confirmed hot water extract of ISBP treatment reduced inflammation and changed responsible metabolites.
Jeong, Inhye,Kang, Sun Kyoung,Kwon, Woo Sun,Kim, Hyun Jeong,Kim, Kyoo Hyun,Kim, Hyun Myong,Lee, Andre,Lee, Suk Kyeong,Bogenrieder, Thomas,Chung, Hyun Cheol,Rha, Sun Young John Wiley and Sons Inc. 2018 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.22 No.12
<P><B>Abstract</B></P><P>Several carcinomas including gastric cancer have been reported to contain Epstein‐Barr virus (EBV) infection. EBV‐associated gastric cancer (EBVaGC) is classified as one of four molecular subtypes of gastric cancer by The Cancer Genome Atlas (TCGA) group with increased immune‐related signatures. Identification of EBV‐dependent pathways with significant biological roles is needed for EBVaGC. To compare the biological changes between AGS gastric epithelial cells and EBV‐infected AGS (AGS‐EBV) cells, proliferation assay, CCK‐8 assay, invasion assay, cell cycle analysis, RT‐PCR, Western blot and ELISA were performed. BI836845, a humanized insulin‐like growth factor (IGF) ligand‐neutralizing antibody, was used for IGF‐related signalling pathway inhibition. AGS‐EBV cells showed slower proliferating rate and higher sensitivity to BI836845 compared to AGS cells. Moreover, invasiveness of AGS‐EBV was increased than that of AGS, and BI836845 treatment significantly decreased the invasiveness of AGS‐EBV. Although no apoptosis was detected, entry into the S phase of the cell cycle was delayed in BI836845‐treated AGS‐EBV cells. In conclusion, AGS‐EBV cells seem to modulate their proliferation and invasion through the IGF signalling pathway. Inhibition of the IGF signalling pathway therefore could be a potential therapeutic strategy for EBVaGC.</P>
Lee, Inhye,Kim, Kuglae,Lee, Sumin,Lee, Seungjun,Hwang, Eunjin,Shin, Kihye,Kim, Dayoung,Choi, Jungki,Choi, Hyunmo,Cha, Jeong Seok,Kim, Hoyoung,Lee, Rin-A,Jeong, Suyeong,Kim, Jeongsik,Kim, Yumi,Nam, Hon Oxford University Press 2018 Journal of experimental botany Vol.69 No.15
<▼1><P>A missense mutation of <I>KARRIKIN-INSENSITIVE2</I>, <I>KAI2</I><SUP><I>ply2</I></SUP>, compromises its ligand-binding activity, which subsequently impairs KAI2-signaling and multiple aspects of light-dependent responses.</P></▼1><▼2><P><B>Abstract</B></P><P>A smoke-derived compound, karrikin (KAR), and an endogenous but as yet unidentified KARRIKIN INSENSITIVE2 (KAI2) ligand (KL) have been identified as chemical cues in higher plants that impact on multiple aspects of growth and development. Genetic screening of light-signaling mutants in <I>Arabidopsis thaliana</I> has identified a mutant designated as <I>ply2</I> (<I>pleiotropic long hypocotyl2</I>) that has pleiotropic light-response defects. In this study, we used positional cloning to identify the molecular lesion of <I>ply2</I> as a missense mutation of <I>KAI2</I>/<I>HYPOSENSITIVE TO LIGHT</I>, which causes a single amino acid substitution, Ala219Val. Physiological analysis and genetic epistasis analysis with the KL-signaling components <I>MORE AXILLARY GROWTH2</I> (<I>MAX2</I>) and <I>SUPPRESSOR OF MAX2 1</I> suggested that the pleiotropic phenotypes of the <I>ply2</I> mutant can be ascribed to a defect in KL-signaling. Molecular and biochemical analyses revealed that the mutant KAI2<SUP>ply2</SUP> protein is impaired in its ligand-binding activity. In support of this conclusion, X-ray crystallography studies suggested that the <I>KAI2</I><SUP><I>ply2</I></SUP> mutation not only results in a narrowed entrance gate for the ligand but also alters the structural flexibility of the helical lid domains. We discuss the structural implications of the Ala219 residue with regard to ligand-specific binding and signaling of KAI2, together with potential functions of KL-signaling in the context of the light-regulatory network in <I>Arabidopsis thaliana</I>.</P></▼2>
( Jeong Eun Kwon ),( Jin Woo Lee ),( Yuna Park ),( Eun-hwa Sohn ),( Eui Su Choung ),( Seon-a Jang ),( Inhye Kim ),( Da Eun Lee ),( Hyun Jung Koo ),( Jong Phil Bak ),( Sung Ryul Lee ),( Se Chan Kang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1
Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at 37℃. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to 500 μg/ml of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL (100 μg/ml) could highly suppress the content of melanin and melanosome formation in B16-F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.
정명훈,윤환민,함인혜,정민영,황완균 중앙대학교 약학연구소 1999 약학 논총 Vol.13 No.-
The constituent of the leaves of Weigela florida var. glabra Caprifoliaceae were studied phytochemically in order to investigate any resources of them, because the leaves of Weigela florida var. glabra has not been studied as medicinal plant. The methanolic extract of them was suspended with water and then partitioned with ethyl ether. The aqueous fraction was submitted to column chromatography on Diaion HP-20 with gradient solvent system as follows; 100% H_2O, 20% MeOH, 60% MeOH, 100% MeOH. 20% MeOH and 60% MeOH fraction were subjected to sephadex LH-20 column chromatography using 15%, 50% MeOH to yield compound Ⅰ, Ⅱ, Ⅲ and Ⅳ. Structures of the four compounds were elucidated by spectroscopic parameters of ^1H-NMR, ^13C-NMR, DT-IR and FAB-MS, and identified as; Compound Ⅰ(Fraxetin), Compound Ⅱ (FRaxin-β-D-glucopyranoside), Compound Ⅲ(Quercetin), and Compound Ⅳ (Quercetin-3-O-β-D-glucopyranosyl(1→2)-β-D-glucopyranoside).
A method for selection of restriction enzymes for sdCAPS marker construction
Jeong, YeSol,Lee, SunYoung,Choi, InHye,Lim, YongPyo,Hur, YoonKang,Staub, Jack E.,Chung, SangMin Blackwell Publishing Ltd 2011 Plant breeding Vol.130 No.3
<P>With 2 figures</P><P><B>Abstract</B></P><P>Development of PCR‐based markers for single‐nucleotide polymorphism (SNP) detection is prerequisite for various genetic analyses. The use of restriction enzymes (REs) following PCR amplification is a common and relatively low cost method for SNP detection. Simple and cost‐effective methodologies for SNP marker development that would enhance the use of SNP‐based technologies are desirable. As an alternative analytical method for selection of REs for recognition of SNP motifs for marker development that does not require computer‐based selection of REs is herein described. Given that only 12 REs are required for the detection of any SNP motif, the method described in this study is relatively inexpensive and technically simple.</P>