Constituents of Salix medicinal plants (II) : Flavonoids from the leaves of Salix gilgiana.

황완균,오인세,정민영,김일혁 한국생약학회 1995 생약학회지 Vol.26 No.1

As a series of the investigation of medicinal resources in plant, the studies were carried out to evaluate the phenolic compounds in the Aerial Part of Salix gilgiana. From the MeOH extract of the Bark, we reported four phenolic compounds ((+)-catechin, 1-O-p-coumaroylglucose, 1-O-feruloylglucose and 4-O-β-D-glucopyranosylacetophenone) and continuously have been isolated two flavone glycoside from the same plant Leaves. From the water fraction of the MeOH extract, Compound I (luteolin-7-O-β-D-glucopyranoside) and Compound II (luteolin-7-O-β-D-glucuronopyranoside) were isolated by column chromatographic separation using Amberlite XAD-2, ODS-gel and Sephadex LH-20, and elucidated physico-chemical evidence (¹H-NMR, ¹³C-NMR, IR, EI-Mass, FAB-Mass, UV, and G.C).

Methyl gallate and chemicals structurally related to methyl gallate protect human umbilical vein endothelial cells from oxidative stress

황완균,박형순,함인혜,오미현,홍남궁,김현기,황동휘,허수영,김태은,박윤규,김재룡,김진우 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.4

Methyl gallate (meGAL) is known as one of major antioxidants. To investigate whether meGAL pro-tects human cells from oxidative stress, meGAL extracted from Korean medicinal plant, Cercis chinensis leaves, was primarily screened using cell viability assay against oxidative stress. Human were treated with three different concentrations of meGAL for indicated time. After or during meGAL treatment, H2O2 was added and incubated. meGAL showed free radical scavenging effect at low concentration (0.02 mM) and cell protective effect against H2O2-mediated oxidative stress. meGAL recovered viability of HUVECs damaged by H2O2-treatment, reduced the lipid peroxidation (LPO) and decreased the internal reactive oxygen species (ROS) level elevated by H2O2meGAL was proven to be very high. Differential display reverse transcription-PCR analysis showed that meGAL upregulated the levels of regulator of chromatin condensation 1, type 1 sigma receptor and phosphate carrier protein expressions, respectively. Based on structural similarity compared with meGAL, 14 chemicals were chosen and viability assay was performed. Four chemicals, haematom-mic acid (56.2% enhancement of viability), gallic acid (35.0%), methylorsellinic acid (23.7%), and syringic acid (20.8%), enhanced more potent cell viability than meGAL, which showed only 18.1% enhancement of cell viability. These results suggest that meGAL and four meGAL-related chemicals protect HUVECs from oxidative stress.

경옥고의 생리활성(III) -염증, 위궤양, 진통 및 정상체온에 미치는 영향-

황완균,오인세,김용빈,신상덕,김일혁,Whang, Wan-Kyunn,Oh, In-Se,Kim, Yong-Bin,Shin, Sang-Duk,Kim, Il-Hyuk 한국생약학회 1994 생약학회지 Vol.25 No.2

The studies were conducted to investigate the inflammation, gastric ulcer, analgesic and homothrmic of experimental animals by KYUNG OK-KO water extract and drink, which is a traditional preparation in Korea. 1. It was exhibited significantly anti-inflammatory effects to acute inflammation on carragennan edema, and preventive and therapeutic effects to chronic inflammation on Freund's complete adjuvant in rats(100, 200, 400 mg/kg). Especially, preventive effect of KYUNG OK-KO water extracts at doses of 100, 200, 400 mg/kg in rats were showed dose-dependantly. 2. Aspirin-induced gastric ulcer were remarkably repaired by all experimental groups of KYUNG OK-KO water extracts. 3. The number of writing syndromes by acetic acid induced also were decreased which MPI's test was increased by tail flick apparatus in mice remarkably, all doses of water extracts(100, 200, 400 mg/kg) and dose-dependantly. 4. Aminopyrine-induced homothermic effects of KYUNG OK-KO water extract(100, 200, 400mg/kg) and drink (0.7, 1.4, 2.1 ml/kg) were significantly evaluated dose-dependantly in 400 mg/kg, 2.1 ml/kg groups.

Natural compounds, fraxin and chemicals structurally related to fraxin protect cells fromoxidative stress

황완균,박형순,함인혜,오미현,남궁홍,김현기,황동휘,허수영,김태영,박용규,김재룡,김진우 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.5

Coumarins comprise a group of natural phenolic compounds found in a variety of plant sources. In view of the established low toxicity, relative cheapness, presence in the diet and occurrence in various herbal remedies of coumarins, it appears prudent to evaluate their properties and applications further. The purpose of this study is to investigate celular protective activity of coumarin compound, fraxin extracted from Weigela florida var. glabbra, under oxidative stress, to identify genes expressed differentially by fraxin and to compare antioxidative effect of fraxin with its struc-the coumarins, protective effects of fraxin against cytotoxicity induced by H2O2 were examined in human umbilical vein endothelial cells (HUVECs). Fraxin showed free radical scavenging effect at high concentration (0.5 mM) and cell protective effect against H2O2-mediated oxidative stress. Fraxin recovered viability of HUVECs damaged by H2O2- treatment and reduced the lipid peroxidation and the internal reactive oxygen species level elevated by H2O2 treatment. Differential display reverse transcrip-tion-PCR revealed that fraxin upregulated antiapo-ptotic genes (clusterin and apoptosis inhibitor 5) and tumor suppressor gene (ST13). Based on structural similarity comparing with fraxin, seven chemicals, fraxidin methyl ether (29.4% enhancement of viability), prenyletin (26.4%), methoxsalen (20.8 %), diffratic acid (19.9%), rutoside (19.1%), xanthyletin (18.4%), and kuhlmannin (18.2%), enhanced more potent cell via-bility in the order in comparison with fraxin, which showed only 9.3% enhancement of cell viability. These results suggest that fraxin and fraxin-related che-micals protect HUVECs from oxidative stress.

여성의 라이프 스타일에 따른 화장품 구매 태도

황완균,이영애,안희진,조연경 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.2

The purpose of this study is to analyze the buy and satisfaction on cosmetics of women with lifestyle. A survey was conducted from May 20 to June 10 in 2009. The statistical analysis methods used to analyze the 330 female subjects were frequency analysis, reliability analysis, factor analysis, cluster analysis, crosstab, and multiple regression analysis. First, women were buying basic cosmetics as well as skin lotion, moisture lotion, eye cream and fuctional cream, and they more invested basic cosmetics than makeup cosmetics. Second, women's the most buying factor on cosmetics is function. Third, the well-being pursuit disposition factors had satisfaction of using on basic and makeup cosmetics, and health concerned group more used basic and makeup cosmetics than other group. 본 연구는 20대~50대 여성을 대상으로 육체적, 정신적으로 건강한 삶을 추구하고 이를 통해 삶의 행복과 만족을 찾으려는 라이프 스타일을 변수로 여성의 화장품 구매 태도에 관하여 알아보았다. 본 연구의 결과에 따른 결론 및 제언은 다음과 같다. 첫째, 여성들은 색조화장품보다는 기초 화장품 구입에 더 많은 비용을 투자 하는 것으로 보여진다. 색조화장품은 많은 연구 대상자들이 3가지 이하를 사용하는 것으로 나타났으나 기초화장품은 4가지 이상 사용하는 대상자가 많은 것으로 보여 스킨과 로션, 영양크림 이외에 주름이나 미백에 효과적인 기능성 화장품에 관심이 높을 것으로 사료된다. 따라서 화장품 회사 및 판매처에서는 여성용 상품 개발 및 판매시 보습, 미백 등 기초화장품의 기능을 높이는데 주력하여 여성의 욕구를 충족시켜야 할 것이다. 둘째, 여성들은 화장품 구매 고려 요인으로 기능성을 가장 높게 생각하고 있는 것으로 나타나 기능성 화장품에 대한 화장품 업계의 노력이 더욱 많이 요구되며, 화장품 판매처에서도 기능성 화장품 위주의 마케팅이 도움이 될 것으로 생각된다. 셋째, 여성들 중에서도 노후를 준비하거나 여가 생활에 투자하고, 삶과 생활이 여유롭다고 여길수록 기초와 색조 화장품의 사용에 만족하고, 다른 집단에 비하여 정기적인 건강검진이나 규칙적인 운동 등으로 건강에 관심이 높은 집단이 기초와 색조화장품 사용을 많이 하고 있었다. 최근 들어 사회생활 및 여가 생활에 적극적이고 건강에 관심을 갖는 여성이 증가하고 있어 이들 계층의 화장품 사용 정도는 더욱 높아질 것이므로 화장품 회사에서는 피부 결점을 보완, 개선하거나 얼굴 결점을 감출 수 있는 화장 기법 등의 정보 제공으로 고객의 마음을 잡아야 할 것이다.

좀민들레의 약효 성분(I) -좀민들레 지상부의 Phenol 성분-

황완균,오인세,이무택,양덕숙,김일혁,Whang, Wan-Kyunn,Oh, In-Se,Lee, Moo-Taek,Yang, Deuk-Sook,Kim, Il-Hyuk 한국생약학회 1994 생약학회지 Vol.25 No.3

For the investigation of medicinal resources from Taraxacum species, the studies were carried out to evaluate the pharmaco-constituents in the aerial part of Taroxacum hallaisanensis, an endemic plant of Korea. From BuOH fraction of the MeOH extract, compound 1 (protocatechuic acid, $C_7H_6O_4,\;3,4-dihydroxy\;benzoic\;acid)$, compound 2 $[C_{22}H_{31}O_6,\;luteolin-7-O-{\alpha}-_L-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-_D-glucopyranoside]$, and compound 3 $[C_{15}H_{20}O_6,\;luteolin-7-O-{\beta}-_D-glucopyranoside]$ were isolated by column chromatographic separation using polyamide and ODS-gel. The structures were elucidated by means of physico-chemical evidences($^1H-NMR,\;{12}^C-NMR$, IR, EI-Mass, FAB-Mass and GC).

갈근류 생약의 puerarin 함량분석 및 품질평가

황완균,정운정,오창환,송효남,이종필,성락선,제금련,장승엽,고성권 한국생약학회 2007 생약학회지 Vol.38 No.2

study was carried out to obtain the basic information that can be used to index arrowroots.(Pueraria species)in fourteen regions of China and Korea. The puerarin content in various arrowroots produced in the different areas were quan-titatively analysed by HPLC. The average of puerarin content of the ‘Galgeun’ (Pueraria lobata) was higher than that of the‘Bungalgeun’ (Pueraria thomsoni) produced in China and Korea. As a result, the order of the puerarin content was 1) Korean.(P. lobata, 0.609%), 2) Chinese puerariae radix.(P. lobata, 0.546%), 3) Chinese puerariae radix.(P. thomsonii,0.066%). This result showed that the content of puerarin in the ‘Galgeun’ (P. lobata) are about 10 times higher than those ofthe ‘Bungalgeun’ (P. thomsonii).Key words HPLC, Pueraria lobata, Pueraria thomsonii, puerariae radix, puerarin.. (.. ). .. (Leguminosae). . Pueraria lobata(WILLD.) OHWI(Pueraria thunbergiana BENTH.). ... ... ... , .. ... .. .. ... , . 5mm. .

여우주머니 잎의 Phenol성 성분(I)

황완균,오인세,함인혜,한덕룡,Whang, Wan-Kyunn,Oh, In-Se,Ham, In-Hye,Hahn, Dug-Ryong 한국생약학회 1994 생약학회지 Vol.25 No.2

For the investigation of medicinal resource in Phyllanthus ussuriensis, the studies were conducted to evaluate the pharmaco-constituents in Phyllanthus ussuriensis, which is used as folk medicine in China. From the hot water extract of leaves, three phenolic compounds were isolated and identified as phloroglucinol, gallic acid and rutin by physico-chemical properties and spectroscopic evidences$(IR,{\;}^1H-NMR,{\;}^{13}C-NMR{\;}and{\;}Mass,{\;}etc.)$.

여성의 로하스 의식 수준 및 사회인구학적 변인에 따른 천연성분 화장품 구매 행동

황완균,이영애 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.3

LOHAS(Lifestyle of Health And Sustainablility) is a lifestyle placed a value on health, environment, self-development, maintainable life. It is not a mere passing vogue but a inevitable trend based on the industrial development and the improvement of consumer's consciousness, at this point of time which is emphasized the importance of environment, continuous consumption and economy. There are the results of the study. First, Korean female consumers' level of consciousness of LOHAS is high. Second, the consciousness change according to the age, income of the family. Altruism is the most effective variable of LOHAS consciousness and the consciousness is high when it is strong. Third, the consumption of natural ingredient cosmetics is not active yet. The results of the study summarized as follows. Korean female consumers' LOHAS consciousness is started with high interest in environment and health and the level is high. It is affected by age, economic status etc. Korean female consumers use common products together with LOHAS one half-and-half and it si affected by, age, marriged state, economic status etc. It is also affected by the altruism and LOHAS consciousness most. To promote the consumption of LOHAS production, we must solve the problem related to the circumstance, how to use and purchase place and improve the consciousness of LOHAS. 최근 소비자들의 건전하고 건강한 삶의 추구는 자신과 가족뿐만 아니라 사회전반적인 책임의식을 강조한 ‘로하스(LOHAS: Lifestyle of Health And Sustainability)'라는 진화된 새로운 트렌드를 창조시켰다. 우리나라의 경우, 로하스가 이슈에 머무르고 있고 로하스층에 대한 연구가 부족한 실정이다. 이에 소비의 주체라 할 수 있는 여성들의 로하스 의식을 살펴보고, 그들의 생활과 연관성이 깊은 화장품 분야에서 로하스와 관련 있는 천연성분 화장품의 구매 행동에 대해 알아보고, 천연 성분 화장품이 생활 속에 정착될 수 있도록 하기 위해 여성들이 생각하는 천연 성분화장품과 관련된 개선 방안에 대해서 알아 보는 것이 중요할 것으로 생각된다. 본 연구를 통해 로하스 의식은 여성들의 천연성분 화장품 소비 수준에 유의한 영향이 있음을 알수 있었다. 즉, 천연 성분 화장품 구매 행동은 로하스 의식을 기반으로 할 것이라는 선행연구를 기초로 한 연구 모형이 타당하다는 결과를 도출했다. 또한 여성 소비자들의 경우 로하스 의식 수준이 매우 높은 것으로 나타나, 여성들의 생활과 매우 밀접한 연관성이 있는 화장품 업계에서도 이러한 시대상을 반영하여 로하스 관련 신 제품의 출시 및 다양한 마케팅 기법을 활용하는 것이 필요할 것으로 보이므로 화장품 업계에서는 소비자들이 안전하고 믿을수 있는 천연 성분 화장품의 유통, 제조 경로를 통해 화장품 업계의 새로운 판로를 모색해야 할 것이다.

중.노년 여성의 생활방식에 따른 한방 화장품 구매행동과 태도

황완균,이영애 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.2

The purpose of this study was to identify purchasing behaviour and attitudes toward oriental cosmetics according to middle, elderly women's lifestyle. Self-administered questionnaires were distributed to 330 women aged 30-70. SPSS was used for data analysis. The results were as follows:1. Factors of lifestyle were service oriented, environmental consciousness, family centered, individuality consciousness, healthy life, information search. Women were clustered into information group, individual group, environmental group, service group, healthy group. 2. Most women had a bit of knowledge and experienced oriental cosmetics. Over 21% of women had intention to use oriental cosmetics. 3. Knowledge and experiences of oriental cosmetics were the most effective factors on the use intention of oriental cosmetics. 본 연구는 중.노년 여성의 한방 화장품 사용 및 구매 실태를 알아보고, 이들의 생활 방식에 따른 한방 화장품 사용 및 태도를 분석하는데 목적이 있다. 이 결과는 화장품 산업에서 중.노년 여성이 타깃인 제품 개발 및 서비스 전략 수립에 도움이 될 것으로 생각된다. 연구 조사는 2009년 10월 2째주부터 4째주 사이에 실시하였으며 연구대상자는 서울, 경기지역에 거주하는 30세~70세의 중.노년 여성 330명이었다. 본 연구에 사용된 질문지는 중.노년 여성의 생활방식, 화장품 구매행동, 한방 화장품 태도, 인구통계적 변인을 측정하는 문항과 한방 화장품 구매 행동 및 태도로 구성되어 있는데, 생활방식에 관한 문항은 선행연구들을 참고로 30문항으로 구성했으며 5점 Likert형으로 측정했다. 한방 화장품 구매행동 및 태도는 선행 연구들을 참고로 구성했으며, 단순선택형이었다. 설문조사 자료를 SPSS PC를 사용하여 분석하였다. 본 연구 결과를 기초로 다음과 같은 결론을 얻을 수 있었다. 한방 화장품 사용의도는 사용경험이나 구매 경험에 따라 높아질 수 있으므로 사용 경험과 구매 경험을 사용의도로 유도할 수 있는 견본 마케팅 또는 체험 마케팅 전략을 구사할 필요성이 부각되었다. 특히 현재 사용하고 있는 화장품을 다른 제품으로 전환하는 과정에는 피부 자극을 일으킬지도 모른다는 위험 요인이 내재되어 있으므로 한방 화장품 견본을 사용해 본 후 안심하고 한방 화장품으로 전환 할 수 있는 계기와 동기를 부여하는 것이 중요하다. 그러므로 한방 화장품업체에서는 적극적으로 중.노년 여성층을 공략하여 시장 점유율을 높여 가야 할 것이다.