Studies of cocktail therapy with multiple cytokines for neoplasia or infections disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Il Seob Shin,Hye Ryon Kim,Myung Jin Nam,Hwa Young Youn 대한수의학회 2001 Journal of Veterinary Science Vol.2 No.2

인삼과 솔잎첨가에 따른 동치미의 성분 변화

김일경,신승렬,정진호,윤광섭,김광수 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

본 연구는 영양성과 약리성을 갖춘 품질이 우수한 동치미의 제조방법을 개발하고자 각종 약리성분을 함유하고 있는 인삼과 솔잎을 첨가하여 숙성 중에 유기산, 당류, 단백질 및 유리 아미노산 등의 변화를 조사하였다. 환원당 함량의 변화는 숙성 1일에 178.9~194.9㎎/100㎖이었으나, 발효 8일에서는 솔잎첨가구는 274.9㎎/100㎖로 증가하여 이후에도 계속 증가하였고, 모든 처리구에서는 발효 15일부터 증가현상을 보였으며, 솔잎첨가구 가 다른 구들보다 환원당 함량이 높았다. 가용성 단백질은 숙성 1일에 3.5~7.5㎎/100㎖이었으며, 숙성됨에 따라 무처리구와 인삼처리구에서는 숙성 29일에 뚜렷이 증가하였으나 솔잎 첨가구는 발효 22일에 60.7㎎/100㎖로 증가한 후 변화가 없었다. 총유기산의 함량은 숙성초기에는 모든 처리구에서 2.79~4.80㎎/100㎖로 낮았으나 숙성말기에는 95.16~109.97㎎/100㎖에 이르렀고 젖산이 85%이상 차지하였다. 유리당은 sucrose, glucose, fructose 3종이었고, sucrose에 비해 glucose와 fructose의 함량이 비교적 높았으며, 모든 처리구에서 유리당 함량은 705.2~943.7㎎/100㎖이었다. 총아미노산은 % 이었고 lysine, histidin, 그리고 tryptophan 3종을 제외한 17종이 동정되었으며, threonine, valine, aspartic aicd, serine, asparagine, glutamic acid, alanine 및 arginine 등의 함량이 많았다. 아미노산 유도체는 53~71%이었고, aminoisobutyric acid, sarcosine, ornithine 등 13종이 동정되었고 특히 γ-aminoisobutyric acid의 함량이 많았으나 숙성 중에 감소한 반면 α-aminoisobutyric acid는 숙성 중에 감소하였다. This paper was studied the effects of ginseng and pine needle on the changes of components in Dongchimi which added ginseng 0.1% and pine needle 0.3% at manufacture, and fermented at room temperature for first days and at 4℃ from 2nd day to 28 day. The contents of reducing sugar increased remarkably in 22 days, those of the Dongchimi added pine needle was highest than those of others. The contents of proteins increased remarkably in the Dongchimi added pine needle in 22 days of fermentation, but increased in others at 29 days of fermentation. The contents of non-volatile organic acids were 2.79~4.80㎎/100㎖ at the late of fermentation, and the content of lactic acid was most than others. Free sugars were composited sucrose, glucose and fructose, and the contents of those were in the range of 227.4~247.0㎎/100㎖ at 8 days of fermentation, then increased 705.2~943.7㎎/100㎖ at 29 days of fermentation. It was identified 17 kinds of free amino acids except lysine, histidine and tryptophan. The contents of free amino acids and amino acid derivatives were 34~47 and 56~71% of total amino acid. The contents of essential amino acid in Dongchimi added ginseng and pine needle were much than control. Especially contents of threonine, valine and isoleucine were much than others, and in the amino acid dervatives, sarcosine, β-aminoisobutyric acid and γ-aminoisobutyric acid were much than others.

Reversal of serologic, immunologic, and histologic dysfunction in mice with systemic lupus erythematosus by long‐term serial adipose tissue–derived mesenchymal stem cell transplantation

Choi, Eun Wha,Shin, Il Seob,Park, So Young,Park, Ji Hyun,Kim, Jong Sung,Yoon, Eun Ji,Kang, Sung Keun,Ra, Jeong Chan,Hong, Sung Hwa Wiley Subscription Services, Inc., A Wiley Company 2012 Vol.64 No.1

<P><B>Abstract</B></P><P><B>Objective</B></P><P>To investigate the efficacy of human adipose tissue–derived mesenchymal stem cell (AD‐MSC) transplantation in systemic lupus erythematosus (SLE) and to determine the optimal transplantation window for stem cells either before or after disease onset.</P><P><B>Methods</B></P><P>(NZB × NZW)F<SUB>1</SUB> mice with SLE were administered human AD‐MSCs (5 × 10<SUP>5</SUP>) intravenously every 2 weeks from age 6 weeks until age 60 weeks, while the control group received saline vehicle on the same schedule. Another experiment was carried out with a different initiation time point for serial transplantation (age 6 weeks or age 32 weeks).</P><P><B>Results</B></P><P>Long‐term serial administration (total of 28 times) of human AD‐MSCs ameliorated SLE without any adverse effects. Compared with the control group, the human AD‐MSC–treated group had a significantly higher survival rate with improvement of histologic and serologic abnormalities and immunologic function, and also had a decreased incidence of proteinuria. Anti–double‐stranded DNA antibodies and blood urea nitrogen levels decreased significantly with transplantation of human AD‐MSCs, and serum levels of granulocyte–macrophage colony‐stimulating factor, interleukin‐4 (IL‐4), and IL‐10 increased significantly. A significant increase in the proportion of CD4+FoxP3+ cells and a marked restoration of capacity for cytokine production were observed in spleens from the human AD‐MSC–treated group. In the second experiment, an early stage treatment group showed better results (higher survival rates and lower incidence of proteinuria) than an advanced stage treatment group.</P><P><B>Conclusion</B></P><P>Serial human AD‐MSC transplantation had beneficial effects in the treatment of SLE, without adverse effects. Transplantation of human AD‐MSCs before disease onset was preferable for amelioration of SLE and restoration of immune homeostasis.</P>

Cloning of canine GM-CSF and SCF genes

Il Seob Shin,Myung Jin Nam,Seong Jun Park,Hwa Young Youn,Hong Ryul Han 대한수의학회 2001 Journal of Veterinary Science Vol.2 No.3

단간 부유 품종의 과실 변색에 영향하는 요인

신일섭(Shin Il Seob),이승구(Lee Seung Koo),박윤문(Park Youn Moon) 한국원예학회 1993 한국원예학회 학술발표요지 Vol.11 No.1

The effects of different pilot-drilling methods on the mechanical stability of a mini-implant system at placement and removal

Il-Sik Cho,HyeRan Choo,Seong-Kyun Kim,Yun-Seob Shin,Duck-Su Kim,Seong-Hun Kim,Kyu-Rhim Chung,John C. Huang 대한치과교정학회 2011 대한치과교정학회지 Vol.41 No.5

Objective: To investigate the effects of different pilot-drilling methods on the biomechanical stability of self-tapping mini-implant systems at the time of placement in and removal from artificial bone blocks. Methods: Two types of artificial bone blocks (2-mm and 4-mm, 102-pounds per cubic foot [102-PCF] polyurethane foam layered over 100-mm, 40-PCF polyurethane foam) were custom-fabricated. Eight mini-implants were placed using the conventional motor-driven pilot-drilling method and another 8 mini-implants were placed using a novel manual pilot-drilling method (using a manual drill) within each of the 2-mm and 4-mm layered blocks. The maximum torque values at insertion and removal of the mini-implants were measured, and the total energy was calculated. The data were statistically analyzed using linear regression analysis. Results: The maximum insertion torque was similar regardless of block thickness or pilot-drilling method. Regardless of the pilot-drilling method, the maximum removal torque for the 4-mm block was statistically higher than that for the 2-mm block. For a given block, the total energy at both insertion and removal of the mini-implant for the manual pilot-drilling method were statistically higher than those for the motor-driven pilot-drilling method. Further, the total energies at removal for the 2-mm block was higher than that for the 4-mm block, but the energies at insertion were not influenced by the type of bone blocks. Conclusions: During the insertion and removal of mini-implants in artificial bone blocks, the effect of the manual pilot-drilling method on energy usage was similar to that of the conventional, motor-driven pilot-drilling method.

Comparison of rhythmic and non-rhythmic aerobic exercises on depression and balance in the elderly

( Il-ho Kwon ),( Jun-young Song ),( Do-ye Kim ),( Je-yeong Son ),( Yu-jin Shim ),( Won-seob Shin ) 물리치료재활과학회 2017 Physical therapy rehabilitation science Vol.6 No.3

Objective: The purpose of this study was to investigate the effects rhythmic and non-rhythmic aerobic exercises on depression and balance of healthy elderly people. Design: Randomized controled trial. Methods: Nineteen older subjects were randomly divided into 2 groups for rhythmic and non-rhythmic aerobic exercises. Both aerobic exercises consisted of functional movements such as turning in opposite directions, and running in place, the exercise consisted of movements that could activate balance. The rhythmic training group initially used music with 8 beats, and then later progressed to 16 beats. Additionally, we adjusted the pace of the music using songs from 125 beats per minute (bpm) to 160 bpm. Both groups were exercised for 50 minutes a day, twice a week, for a total of 8 weeks. We measured the condition of the patients before the intervention, and after 8 weeks of intervention. The Beck depression inventory (BDI) was used to measure the degree of depression. The Berg balance scale (BBS) was used to measure static and dynamic balance ability. We measured the subject`s subjective balance confidence using the fall efficacy scale (FES). Results: Both groups showed significant improvement in BDI, BBS, and FES (p<0.05). The rhythmic aerobic exercise group showed a significant improvement only in the BBS change values compared to the non-rhythmic group (p<0.05). Conclusions: According to this study, both rhythmic and non-rhythmic aerobic exercises resulted in significant improvement in the degree of depression and balance ability of the elderly. The rhythmic aerobic exercise was more effective for dynamic balance ability.

정상인에서 도플러 조직 영상(Doppler Tissue Imaging)을 이용한 승모판륜 속도에 관한 연구

문찬일,최재웅,조용범,신원용,송창섭 대한순환기학회 2001 Korean Circulation Journal Vol.31 No.7

S-80 Clinical outcomes of Lower gastrointestinal bleeding

( Hyoung Il Choi ),( Min Seob Kwak ),( Jae Myung Cha ),( Yong Jae Han ),( Jin Young Yoon ),( Jung Won Jeon ),( Hyun Phil Shin ),( Kwang Ro Joo ),( Joung Il Lee ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Goals:?The aim of this study was to identify the clinical outcomes of lower gastrointestinal bleeding (LGIB) and predictors of poor outcomes in LGIB compared with those of upper gastrointestinal bleeding (UGIB).?Background:?The incidence of LGIB are increasing, however, predictors of outcomes in LGIB are not as well-defined as in UGIB.?Study:?This study was performed in patients with LGIB or UGIB who underwent endoscopic procedures between July 2006 and February 2013. Propensity score matching was used to improve comparability between LGIB and UGIB groups.The clinical outcomes and predictors of 30-day rebleeding and mortality rate were analyzed between two groups.?Results:?In total, 601 patients affected with UGIB (n=500) or LGIB (n=101) were finally eligible, and 202 patients with UGIB and 101 patients with LGIB were analyzed after 2:1 propensity score matching. The 30-day rebleeding and mortality rate were 9.9% and 4.5% for UGIB group and 16.8% and 5.0% for LGIB group, respectively. After logistic regression analysis, Rockall score (p=0.013) and CRP (p=0.047) were significant predictors of 30-day mortality, however, nothing predicted rebleeding in patients with LGIB. Conclusions:?The clinical outcomes of LGIB are not better than those of UGIB. Clinical Rockall score and serum CRP level may be used as a predictor of 30-day mortality in the patients with LGIB.

근골격계질환 예방프로그램의 필요성과 자동차 부품 제조업 근로자들의 근력과 자세의 특성 연구

권일호 ( Il-ho Kwon ),신원섭 ( Won-seob Shin ) 대한물리의학회 2019 대한물리의학회지 Vol.14 No.4

PURPOSE: This study examined the necessity of a musculoskeletal injury-prevention program for automobile parts manufacturing workers by assessing the body composition, muscle strength, and posture according to sex and age. METHODS: Two hundred eighty-two workers (men, 218; women, 64; mean age, 38.97 years; and mean work tenure, 10.29 years) participated in this study. They completed a questionnaire on their general characteristics, excluding personal identification codes. The participants were evaluated for body composition, muscle strength, and posture using InBody, a hand dynamometer, and the Image J program through photography, respectively. An independent t-test and one-way analysis of the variance were used for the comparisons according to sex and age, respectively. RESULTS: Significant sex-related differences in muscle strength and grip force were observed (p<.05). In addition, the left-side pelvic inclination was significantly higher in the female participants than in the male participants (p<.05). Significant age-related differences in the skeletal muscle mass and muscle strength were observed around 40 years (p<.05). Significant correlations were observed among the body composition (skeletal muscle and body fat masses), muscle strength, and posture (pelvic inclination; p<.01). CONCLUSION: Significant differences in pelvic inclination by sex and muscle strength by age were found, even in healthy workers. Additional posture and muscle strength measurements will be needed for workers who participate in the musculoskeletal injury prevention program because they are exposed to an incorrect posture for many hours or in many repetitive tasks.