LPS로 염증 유도된 RAW 264.7세포에 대한 참콩풍뎅이(Popillia flavosellata) 에탄올 추출물의 항염증 효과

윤영일(Young-Il Yoon),황재삼(Jae-Sam Hwang),김미애(Mi-Ae Kim),안미영(Mi Young Ahn),이영보(Young-Bo Lee),한명세(Myung Sae Han),구태원(Tae-Won Goo),윤은영(Eun-Young Yun) 한국생명과학회 2015 생명과학회지 Vol.25 No.9

본 연구에서는 참콩풍뎅이(Popillia flavosellata) 에탄올 추출물(PFE)의 항염증 효능을 분석하기 위해 PFE를 농도별(500, 1,000, 2,000 ㎍/ml)로 대식세포인 RAW 264.7에 처리 시 최고 처리농도인 2,000 ㎍/ml까지 통계적인 유의성 있는 독성이 없음을 확인하였다. LPS (100 ng/ml)로 염증 유도된 RAW 264.7 세포에 PFE를 농도별(500, 1,000, 2,000 ㎍/ml)로 동시 처리 시 농도 의존적으로 염증성사이토카인인 TNF-α와 IL-6의 단백질 생성을 통계적인 유의성(p<0.001)있게 억제함을 확인하였다. 또한 염증 유도된 RAW 264.7 세포에 PFE 동시 처리 시 NF-κB p65의 핵으로 이동이 차단됨과 iNOS와 COX-2의 단백질 발현을 감소시키는 것을 확인하였다. 이상의 연구결과를 통해 참콩풍뎅이는 염증에 의해 활성화된 TLR-4 신호전달과정을 조절하는 NF-κB p65의 활성과 염증성사이토카인 TNF-α와 IL-6의 생성 및 염증성효소 iNOS와 COX-2의 생성을 억제하는 항염증 효능이 있음을 확인하였다. The beetle Popillia flavosellata has been no reported its functional effects. In this study, we investigated the anti-inflammatory effect of P. flavosellata ethanol extract (PFE) on RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for the induction of inflammation. First, we examined the cytotoxicity of PFE in the RAW 264.7 cells at a concentration of 2,000 μg/ml or less. To evaluate the anti-inflammatory effects of PFE, we investigated the expression levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and proinflammatory enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether PFE inhibited the translocation of nuclear factor kappa B (NF-κB) p65 into the nucleus in the LPS-induced RAW 264.7 cells. We found that the protein levels of TNF-α and IL-6 were decreased in the LPS-induced RAW 264.7 cells after the treatment with PFE in a dose-dependent manner. In addition, we confirmed that PFE inhibited the translocation of NF-κB p65 into the nucleus, as well as the protein expression levels of iNOS and COX-2. Accordingly, we propose that PFE exerts an anti-inflammatory effect through the down-regulation of NF-κB p65, TNF-α, IL-6, iNOS, and COX-2 via the toll like receptor (TLR)-4 inflammatory signaling pathway.

LPS로 유도된 RAW 264.7세포에 대한 벼메뚜기(Oxya chinensis sinuosa) 에탄올 추출물의 항염증 효과

윤영일(Young-Il Yoon),정미연(Mi Yeon Chung),황재삼(Jae-Sam Hwang),구태원(Tae-Won Goo),안미영(Mi-Young Ahn),이영보(Young-Bo Lee),한명세(Myung-Sea Han),윤은영(Eun-Young Yun) 한국생명과학회 2014 생명과학회지 Vol.24 No.4

본 연구에서는 벼메뚜기 에탄올 추출물의 항염증 효능을 분석하기 위해 LPS로 염증 유도된 RAW 264.7 세포를 이용하였다. OCE의 항염증 효능을 확인 하기 위해서, 염증 유도된 RAW 264.7 세포에 대해 OCE 농도 의존적으로 염증성 사이토카인인 TNF-α와 IL-6의 유전자발현 및 단백질 생성을 감소시킴을 real-time PCR과 ELISA로 확인하였다. 또한, NF-κB p65의 핵으로 이동이 차단됨을 면역형광염색으로 확인하였으며, iNOS와 COX-2 단백질 발현을 감소시키는 것을 Western blot 분석으로 확인하였다. 이상의 연구결과를 통해 벼메뚜기는 염증에 의한 NF-κB p65의 활성과 TNF-α와 IL-6의 생성과 iNOS 및 COX-2의 발현을 억제하는 항염증 효능을 갖고 있는 것을 확인하였다. Although the grasshopper Oxya chinensis sinuosa has long been used as food in Korea, there is little data on its functional effects. In this study, we investigated the anti-inflammatory effect of O. c. sinuosa ethanol extract (OCE) in RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for induction of inflammation. First, we determined that there is no cytotoxicity at 2,000 μg/ml or less of OCE in RAW 264.7 cells. To evaluate the anti-inflammatory effects of OCE, we investigated expression levels of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and pro-inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclo-oxygenase- 2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether OCE could inhibit translocation of NF-κB p65 into the nucleus in LPS induced RAW 264.7 cells. As a result, we found that the mRNA and protein levels of TNF-α and IL-6 decreased in LPS-induced RAW 264.7 cells after treatment with OCE in a dose-dependent manner. In addition, we confirmed a 2,000 ug/ml concentration of OCE inhibited translocation of NF-κB p65 by immunnostaining and Western blot analysis, and a decrease in the protein expression levels of iNOS and COX-2. Accordingly, we suppose that OCE has an anti-inflammatory effect through down-regulation of TNF-α, IL-6, iNOS, and COX-2 related to NF-κB p65 inflammatory signaling pathways.

IRAK4 as a Molecular Target in the Amelioration of Innate Immunity–Related Endotoxic Shock and Acute Liver Injury by Chlorogenic Acid

Park, Sun Hong,Baek, Seung-Il,Yun, Jieun,Lee, Seungmin,Yoon, Da Young,Jung, Jae-Kyung,Jung, Sang-Hun,Hwang, Bang Yeon,Hong, Jin Tae,Han, Sang-Bae,Kim, Youngsoo American Association of Immunologists 2015 Journal of Immunology Vol. No.

<P>Mice lacking the IL-1R-associated kinase 4 (IRAK4) are completely resistant to LPS-induced endotoxic disorder or the TLR9 agonist CpG DNA plus D-galactosamine-induced acute liver injury (ALI), whereas wild-type strains succumb. However, translational drugs against sepsis or ALI remain elusive. Lonicerae flos extract is undergoing the clinical trial phase I in LPS-injected healthy human volunteers for sepsis treatment. In the current study, chlorogenic acid (CGA), a major anti-inflammatory constituent of lonicerae flos extract, rescued endotoxic mortality of LPS-intoxicated C57BL/6 mice, as well as ameliorated ALI of LPS/D-galactosamine-challenged C57BL/6 mice. As a mechanism, CGA inhibited various TLR agonist-, IL-1 alpha-, or high-mobility group box-1-stimulated autophosphorylation (activation) of IRAK4 in peritoneal macrophages from C57BL/6 or C3H/HeJ mice via directly affecting the kinase activity of IRAK4, a proximal signal transducer in the MyD88-mediated innate immunity that enhances transcriptional activity of NF-kappa B or AP-1. CGA consequently attenuated protein or mRNA levels of NF-kappa B/AP-1 target genes encoding TNF-alpha, IL-1 alpha, IL-6, and high-mobility group box-1 in vivo under endotoxemia or ALI. Finally, this study suggests IRAK4 as a molecular target of CGA in the treatment of innate immunity-related shock and organ dysfunction following insult of various TLR pathogens from bacteria and viruses.</P>

골수이식 후 사이토카인과 골교체 생화학적표지자의 변화 및 상관관계

민우성,강무일,한제호,강성구,오기원,이원영,김혜수,문성대,손현식,신완식,김춘추,윤건호,차봉연,이광우,손호영 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.1

Background : Loss of bone mass is usually detected after BMT. The causes of bone loss are related with gonadal dysfunction and immunosuppressants. Cytokines, especially IL-6, play an important role in the pathogenesis of postmenopausal osteoporosis. However, the pathogenetic role of cytokines in post-BMT bone loss is unknown and data on the changes of cytokines in accordance with bone turnover markers are scarce. The aim of this study is to assess the relationship of bone turnover markers and cytokines of peripheral blood and bone marrow before and after allogeneic BMT. Methods : This prospective study included two analyses. The first was a study of 46 BMT recipients, examining the relationship between bone turnover markers and cytokines of serum which were measured before and 1, 2, 3, 4 week and 3 months after BMT. The second was a study of 14 BMT patients, measuring bone marrow plasma cytokines such as IL-6 and TNF-? at post-BMT 3 week and bone turnover marker at the same time to assess the relationship beween two parameters. Results : Serum ICTP, bone resorption marker, increased progressively until 4 weeks (peak) after BMT and then decreased thereafter. Serum osteocalcin, bone formation marker, decreased progressively until 3 weeks after BMT and then increased thereafter. There was positive correlation between serum ICTP and bone marrow IL-6 levels at the post-BMT 3 week with a statistical significance, but the correlation between bone turnover markers and bone marrow TNF-? or peripheral blood cytokines was not found. Conclusion : Our data suggest that the progressive increase of bone resorption after BMT is related with the increase of bone marrow IL-6, which is a potent stimulator of bone resorption in vivo(J Kor Soc Endocrinol 15:85-96, 2000).

Adrenomedullin inhibits MAPK pathway-dependent rheumatoid synovial fibroblast-mediated osteoclastogenesis by IL-1 and TNF-α

Yun, Hee-Jin,Lee, Eun-Gyeong,Lee, Sang-Il,Chae, Han-Jung,Yoo, Wan-Hee Springer-Verlag 2009 Rheumatology International Vol.29 No.10

기포탑내에서 비뉴튼류의 기-액간 물질이동

윤영웅,김상렬,최일곤,한승완 東亞大學校 1992 東亞論叢 Vol.29 No.1

비뉴튼류인 CMC수용액중의 기체체류량과 용량액상물질전달계수를 분산판 구멍의 면적비와 용액의 물성에 따라 실험하였다. ε??와 K??a의 값은 CMC의 농도가 증가할 수록 감소하나 구멍의 수가 3∼8개 범위에서는 분산판의 구멍 면적비에 거의 영향이 없었다. 실험에 의하여 column에 적용될 수 있는 ε??와 K??a에 대한 식을 제안 하였다. The effect of properties of CMC-solution and area ratio of distributor hole on gas holdup and volu-metric liquid-phase mass transfer coefficient in a bubble column have been experimentally studied. The values of ε?? and K??a were decreased with increasing concentration of CMC and were scarcely affected by the area ratio of distributor in the range of the 3∼8 hole number. Correlations for ε?? and K??a were formulated from the experimental result.

철근콘크리트 장방형 전단벽의 비선형 유한요소 해석

윤현도,한민기,김선우,박완신,양일승,한병찬 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.2

The major objective of the present study is to conduct parametric study and to propose a finite element analysis(FEA) technique of the reinforced concrete rectangular shear walls using ABAQUS under constant axial and monotonic lateral load. A FEA of the reinforced concrete walls are different to aspect ratio and a detailed finite element models are created to study the post-cracked mode of failure in plastic region of the walls. The proposed models account for the effect of material inelasticity, concrete cracking, and geometric nonlinearity. The prossed FEA model is verified by Lefas's benchmark data and validated through comparisons with experiment result of reinforced concrete shear walls. Finally, the study considered various parameters and compared with ACI 318-02 to confirm accuracy of this study: aspect ratio, axial force ratio, vertical and horizontal reinforcement ratio.

BMS 수리모형하에서 시스템의 수리효과 및 고장강도함수의 모수추정

윤원영,정석주,정일한,김종운,정상욱 한국경영과학회 2000 韓國經營科學會誌 Vol.25 No.4

Estimation problems of parameters of the failure process and the repair effect in repairable systems are considered. We propose estimation procedures in repairable systems without preventive maintenances. The fallure process is modeled by a proportional age reduction model (Brown. Mahoney, Sivazlian [5]) which is able to consider both aging and repair effects. Maximum likelihood method is used to estimate the repair effect and parameters of intensity function simultaneously. Simulations are performed to evaluate the accuracy of estimators. A numerical example is also presented.

복합섬유 보강 고인성 시멘트 복합체의 특성

윤현도,양일승,한병찬,福山洋,전에스더,문연준 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

In this paper, workability, fiber dispersion. compressive strength and splitting strength of hybrid fiber reinforced cement composites(HFHCC) have been investigated by means of Korean Standard (KS). Two type of HFRCC were constructed using fiber combinations of Polyethylene(PE) and steel fibers. Polyvinyl alcohol(PVA) and steel fibers. Test results showed that the fibers, when used in a hybrid form. could result in superior composite performance compared to their individual fiber reinforced cement Composites. Synthetic micro fibers. such as PE and PVA. are effective at imparting fiber dispersion and ductility to the steel fiber (five twisted steel fiber) reinforced cement Composite.

초등학생의 인지양식에 따른 검색전략비교

윤미소,김한일 한국컴퓨터교육학회 2003 컴퓨터교육학회 논문지 Vol.6 No.3

인터넷에 많은 정보가 있지만, 검색자가 자신이 필요한 정확한 정보를 찾기는 쉽지 않다. 따라서 정보를 효율적으로 수집, 분석, 활용하는 능력을 길러주는 교육이 요구된다. 일반적으로 검색자들은 저마다의 방법을 통해 정보 검색 과정을 수행하므로, 이때 검색된 결과물은 개인에 따라 상이하게 나타난다. 본 연구는 초등학생의 정보 검색 활동을 관찰하여 검색자의 개인별 특성, 특히 인지 양식에 따른 정보 검색 전략과 검색 결과의 차이를 분석한다. 또한 이를 통해 정보 검색 능력 신장 및 정보 검색 시스템의 효율성 제고를 위한 교육 방안을 제안한다. The popularization of the Internet brought about easy access to a huge amount of information, yet it is hardly easy for one to find information in need. Therefore, information users must have information retrieval abilities to gather, analyze and utilize data in efficient ways. In general the information retrieval strategies of each user are all different, and consequently the retrieved result also significantly varies from one to another. This paper describes an experimental study on information retrieval behaviors in elementary students, and analyzes the variation in retrieval strategies and results by examining cognitive patterns based on their personal characteristics, specifically their cognitive patterns. As a research result we propose a set of education requirements which can improve students’ information retrieval abilities and the efficiency of the information retrieval system.