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Research on Fluid Analysis Simulator for Elastic Oscillating Fin of Biomimetic Underwater Robots
Tomokazu Nakamura,Ikuo Yamamoto 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper describes about researchon fluid analysis simulator for elastic oscillating fin of biomimeticunderwater robots. Authors has been researching biomimetic underwater vehicle such as flapping wing type. In suchsystem, new concept of fluid analysis simulator is required because of the unique fluid characteristics of elasticoscillating fin. First of all, this paper shows basic characteristics of flapping wing type underwater vehicle as typicalcase of elastic oscillating fin. Second of all, it shows theoretical problem of conventional fluid analysis simulator, andthen, also proposes new concept of fluid analysis simulator for elastic oscillating fin to solve this problem.
Kynet Kong,Ikuo Nakamura,So Makabe,Valentine Otang Ntui,Raham Sher Khan 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2
Fusarium wilt is a soil-borne disease causingsubstantial yield losses in various crops and vegetables. Wehave previously reported the synthetic chitinase (NIC) gene(1.2 kb), in which codon usage of fungus, replaced withthat of plant, conferred resistance against Botrytis cinerea. In this study, the NIC or GUS gene was linked to two rootspecificpromoters, LjNRT2 or AtNRT2.1 (nitrate transporter2), derived from Lotus japonica and Arabidopsisthaliana, respectively. Transgenic tobacco lines expressingLjNRT2-GUS and LjNRT2-NIC, and tomato lines expressingAtNRT2.1-NIC, were produced by Agrobacteriummediatedtransformation. GUS histochemical staining wasobserved in vascular regions of the roots but was conspicuouslyabsent in the leaves of transgenic plants. Westernblot analysis showed the production of NIC proteins inthe roots but not in the leaves of transgenic tobacco andtomato lines. These results indicate that LjNRT2 and At-NRT2.1 promoters expressed transgenes in a root-specificmanner. When in vitro whole plant resistance assay againstFusarium oxysporum was conducted, transgenic plantsshowed increased levels of resistance compared to nontransgenicplants. Antifungal activities of the root extractagainst spore germination of F. oxysporum showed lowerCFU (colony-forming unit) than those of the leaf extract. Root colonization assay against F. oxysporum showedmuch lower CFU values in the roots of transgenic plantsthan in those of non-transgenic plants. These results suggestthat NIC gene triggered by the root-specific promoterssuccessfully expressed only in the roots and conferredincreased levels of resistance against the root pathogen,F. oxysporum.
Research on network system of unmanned flying observation robot
Naohiro Inagawa,Ikuo Yamamoto,Tomokazu Nakamura 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper describes about network system of un-manned flying observation robot which can be used to survey disaster area and transmit real time video image to distanced headquarter. In addition, I performed the field experiment that assumed the disaster spot.
Ken Hoshikawa,Ikuo Nakamura,Satoshi Endo,Shingo Mizuniwa,So Makabe,Hiroko Takahashi 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3
Genes encoding pathogenesis-related proteins,such as degrading enzymes of fungal cell wall polysaccharides,have been used to confer enhanced resistance to fungal pathogens of various plants. A new type of endo-bmannanase gene, amn5A, was isolated from alkaliphilic Bacillus strain (JAMB-602) found in deep-sea sediment. The AMN5A mannanase is active over a wide pH range (pH 7–10) and stable at high temperature. In this study,transgenic tobacco plants expressing the amn5A gene were generated using Agrobacterium-mediated transformation. Polymerase chain reaction (PCR) analysis revealed that the amn5A gene was integrated into the genome of transgenic tobacco plants. Southern blot analysis showed that transgenic plants contained 1–6 copies of amn5A transgenes in their genome. Expression of the amn5A transgene was confirmed by reverse transcription-PCR analysis. Leaf extracts from the transgenic plants showed degradation activity of Konjak mannan. Antifungal assay of detached leaves and in vitro whole plantlets indicated that transgenic plants expressing amn5A gene acquired enhanced resistance to the soil-borne pathogenic fungus, Fusarium oxysporum,compared to untransformed control plants.