http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ken Hoshikawa,Ikuo Nakamura,Satoshi Endo,Shingo Mizuniwa,So Makabe,Hiroko Takahashi 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3
Genes encoding pathogenesis-related proteins,such as degrading enzymes of fungal cell wall polysaccharides,have been used to confer enhanced resistance to fungal pathogens of various plants. A new type of endo-bmannanase gene, amn5A, was isolated from alkaliphilic Bacillus strain (JAMB-602) found in deep-sea sediment. The AMN5A mannanase is active over a wide pH range (pH 7–10) and stable at high temperature. In this study,transgenic tobacco plants expressing the amn5A gene were generated using Agrobacterium-mediated transformation. Polymerase chain reaction (PCR) analysis revealed that the amn5A gene was integrated into the genome of transgenic tobacco plants. Southern blot analysis showed that transgenic plants contained 1–6 copies of amn5A transgenes in their genome. Expression of the amn5A transgene was confirmed by reverse transcription-PCR analysis. Leaf extracts from the transgenic plants showed degradation activity of Konjak mannan. Antifungal assay of detached leaves and in vitro whole plantlets indicated that transgenic plants expressing amn5A gene acquired enhanced resistance to the soil-borne pathogenic fungus, Fusarium oxysporum,compared to untransformed control plants.
Chloroplastic NAD(P)H Dehydrogenase Complex and Cyclic Electron Transport around Photosystem I
Tsuyoshi Endo,Satoshi Ishida,Noriko Ishikawa,Fumihiko Sato 한국분자세포생물학회 2008 Molecules and cells Vol.25 No.2
Recent molecular genetics studies have revealed that cyclic electron transport around photosystem I is essential for normal photosynthesis and growth of plants. Chloroplastic NAD(P)H dehydorgenase (NDH) complex, a homologue of the complex I in respiratory electron transport, is involved in one of two cyclic pathways. Recent studies on the function and structure of the NDH complex are reviewed
Okuyama, Masaru,Endo, Jun,Take, Seisho,Itoi, Yasuhiko,Kambe, Satoshi 한국부식방식학회 2002 Corrosion Science and Technology Vol.31 No.6
Utilizing of Ni-Ti shape memory alloy for implant materials has been world-widely studied. It is, however, known that Ni-Ti alloy is easily attacked by chloride ion contained in body liquid. To prevent Ni dissolution, the authors tried to coat the alloy surface with titanium metal by means of plasma-spray coating method. The plasma coating films resulted in rather accelerating pitting corrosion because of their high porosity. Therefore, sealing of the porous films was required. In order to solve this problem and satisfy prolonged lifetime in the body, the authors tried to use the vacuum evaporation technique of titanium metal. Two types of Ti vacuum evaporation procedures were employed. The one was to cover a thin film on Ni-Ti alloy surface prior to massive Ti plasma spray coating. The other was to first coat plasma spray films on Ni-Ti alloy and then to cover them with vacuum evaporation films of Ti. Protective ability against pitting corrosion was examined by electrochemical polarization measurement in physiological solution and the coating films were characterized by microscopic and SEM observation and EPMA analysis. Vacuum evaporation thin films could not protect Ni-Ti alloy from pitting corrosion. In the case of plasma spray coating over the Ti vacuum evaporation thin film, the substrate Ni-Ti alloy could not be better protected. On the contrary, vacuum evaporation of Ti over the porous plasma spray coating layer remarkably improved corrosion protective performance.
Development of a Simulation System for the Spread of Northern Hemisphere Forest Fires
Wataru Uema,Satoshi Endo,Yuhei Akamine,Keiji Kimura,Toshihisa Honma 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
In this research, we propose a method to search appropriate parameters to target are ausing the genetical gorithm for forest fire simulation. We have found that the search performance can begreatly changed depending on the characteristic of input data from experiments using test data sets.
Leaching of Radio-cesium and Other Elements from Cement Solidified MSWI-APC Residues
( Masato Yamada ),( Kazuto Endo ),( Masahiro Sato ),( Satoshi Obokata ),( Tokichiro Yamaguchi ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.1
The cement solidification is a method to dispose waste containing hazardous substances, such as heavy metals and radionuclides. Recently, cement solidified waste landfills for municipal solid waste incinerators (MSWIs) ash and their air pollution control (APC) residues have been operated in Japan. Also, the radio actively contaminated off-site waste caused by the accident of the Fukushima Dai-Ichi nuclear power plant at above a certain level should be disposed to landfill after solidified by cement. The land disposal of cement solidified waste can provide a containment structure to waste itself in the initial and control fluxes of pollutants to environment in the longer term. Test pieces of cement solidified MSWI-APC residues were made under actual landfill conditions and their leachability of pollutants and structural strength were investigated. Two kinds of MSWI-APC residues were tested. One (hot sample) is APC residues from a MSWI ash melting plant and contaminated by radio-cesium. Another (cold sample) is APC residues from a fluidized bed MSWI. Agents of 6 hazardous inorganics and stable cesium were spiked to the latter one. Both APC residues had been treated by chelate agents at plants before sampling. Same amount of APC residues and the Portland blast-furnace cement class B were mixed at 19 to 38% of the water cement ratio. A certain portions of mixtures were injected to mold of 110 mm diameter, pressed at 0.03 to 25 ton/m<sup>2</sup>. These processes were repeated until height of test pieces raising to 300 mm. Formed test pieces were held in molds and pressed heads for 7 to 19 days. Demolded test pieces were cured for 7 to 28 days in the air. The uniaxial compressive strengths of cement solidified test pieces molded under 25 ton/m<sup>2</sup> were 22 to 71 N/mm<sup>2</sup>. Permeability coefficients of test pieces molded at 25 ton/m<sup>2</sup> were less than 1.59 ×10<sup>-12</sup> cm/sec. These were satisfied conditions in the structural landfill standard in Japan. The tank leaching test with stirring for hot samples showed that around 90% of radio-cesium was leached in 6 hours. Less than 4% of radio-cesium was leached from cement solidified test pieces of hot samples. Leaching ratios for test pieces of hot samples obtained by the tank leaching test without stirring for 32 day were 9 to 17%. Leaching ratios for test pieces of spiked cold samples obtained by same test were 0.002% for total mercury form pieces loaded at 0.003 and 3 ton/m2, 0.4% for cesium from pieces loaded at 0.003 ton/m<sup>2</sup> and less than 0.04% from pieces loaded at 3 and 25 ton/m<sup>2</sup>. All other substances were not detected in leachate. These results suggested that higher compaction would be effective to suppress leaching of substances from the cement solidified waste. Avoidance of contacting the naked surface of cement solidified waste to water would also be important to keep containment of hazardous materials in the waste. This study was partly supported by the Environment Research and Technology Development Fund (3K113015) of Ministry of the Environment, Japan.
정어리표피중에 존재하는 지방산화촉진물질의 검색확인 및 그 특성구명
조순영,모리사토시,엔도야스시,후지모토겐시로 한국수산학회 1992 한국수산과학회지 Vol.25 No.6
정어리표피 중에 존재하는 지방산화촉진물질의 검색확인 및 그 특성구명을 시도하였다. 정어리표피중의 산화촉진물질은 0.05M 인산염 완충액으로 거의 모두 추출되었고, 이 조추출액에 대해 Sephadex G-200, DEAE-Sephadex A-50 및 CM-Sephadex A-50 등으로 연속적인 분리를 행하였다. 정어리표피 중의 주 산화촉진물질은 열에 극히 불안정하고, 지방산화촉진의 최적 작용 pH가 7이며, 특징적인 Sohret흡수를 나타내는 점 등으로부터 햄을 가진 효소임을 알 수 있었다. 특히 이들은 기질인 유리 및 에스테르화 리놀산에 모두 잘 작용하여 빠른 속도로 공역과산화물을 형성하였다. 이상의 결과로부터 정어리표피 중의 주 지방산화촉진물의 본태는 새로운 형태의 리폭시지네이스유사 효소인 것으로 추정되었다. Lipid prooxidants in sardine skin was characterized. Prooxidants in the sardine skin extract with 0.05M phosphate buffer was purified by successive chromatography on Sephadex G-200, DEAE-Sephadex A-50 and CM-Sephadex A-50. Prooxidants of sardine skin exist mainly in the intermediate molecular weight fractions. Observations of the thermounstability and optimum pH(pH 7.0) suggest that the major prooxidants are enzymes and hemoproteins. They can oxidize well both free and esterified linoleic acid and form conjugated hydroperoxides. From these results, the major prooxidants in sardine skin are assumed to be lipoxygenase-like enzymes.