Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

미백보조광 조사가 치아미백의 효과에 미치는 영향

박종현,신혜진,박덕영,박세희,김진우,조경모 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.2

The aim of this study was to evaluate the influence of light energy on the tooth whitening effect of bleaching agent in vitro. Extracted human mandibular molars were sectioned to 2 fragments(mesial distal) and lingual portions of crown were used in this study. All specimens were stained using a red wine for 24 hours and immersed in artificial saliva. Specimens divided into four groups, group 1 and 2 light-activated by LumaCool (LED, LumaLite. Inc. Spring Valley, USA) group 3 and 4 light-activated by FlipoWhite2 (Plasma acr lamp, Lokki Australia) Group 1 and 3 bleached with LumaWhite(LumaLite, Inc.. Spring Valley USA), group 2 and 4 bleached with Polaoffice(SDI, Victoria, Australia). Bleaching treatment Performed during 10 minutes every 24 hours and repeated 6 times. During bleaching treatment, distal fragments was light-activated(L) but mesial fragments was not(NL). Shade assessment employed before and after bleaching treatment using spectrophotemeter. The results of the change in shade was compared and analysed between NL and L by using paired-sample T test with 95 % level of confidence. There were no significant differences between NL and L with a few exceptions. In group 2, a^(*) value more change in L, in group 3, b^(*) value more change in L in gioup 4, a(*) value less change in L. After bleaching L^(*) value and ΔE increased in all groups and the value of a^(*) and b^(*) decreased in all groups. Within the limitation of this test conditions, the results of this study indicate that the light energy has no obvious improving impact on the tooth whitening effect of a bleaching agent. 이 연구의 목적은 미백제의 치아미백효과에 미백보조광의 빛에너지의 영향을 평가하는 것이다. 발거된 하악 대구치 치관의 설면을 실험에 사용하였고, 하나의 대구치에서 근심과 원심으로 구분되는 2개의 치아 절편을 얻었다. 모든 시편을 24시간동안 적포도주에 보관하여 착색을 유도하고, 인공타액에 보관하였다. 시편들은 미백광조사기와 미백 powder의 종류에 따라 4개의 실험군으로 나뉘었으며, 실험군은 다음과 같다. Group 1: LumaCool로 광조사를 시행, LumaWhite를 사용 Group 2: LumaCool로 광조사를 시행, Polaoffice를 사용 Group 3: FlipoWhite 2로 광조사를 시행, LumaWhite를 사용 Group 4: FlipoWhite 2로 광조사를 시행, Polaoffice를 사용 미백처치는 10분간 매 24시간마다 총 6회 시행하였고, 미백처치시 동일한 치아에서 얻어진 두 개의 시편 중 원심시편에는 미백광조사를 시행한 반면, 근심시편에는 미백광조사를 시행하지 않았다. 색조의 평가는 spectrophotometer를 사용하여 매 미백처지 전과 후에 시행하였고, 근심측과 원심측에서 얻어진 색조 변화 차이를 paired-sample T test를 이용하여 95%의 신뢰수준으로 비교하였다. 미백처치 후 모든 실험군에서 L^(*) value와 ΔE의 증가와 a^(*) value와 b^(*) value의 감소가 관찰되었다. Group 2 원심절편의 a^(*) value와 group 3 원심절편의 b^(*) value에서의 더 큰 변화와 group 4의 원심절편에서의 a^(*) value가 좀 더 적은 변화를 제외하고, 모든 실험군에서 근심절편과 원심절편사이에서 색조변화의 차이는 통계적 유의성을 보이지 않았다. 이상의 연구 결과에서 미백보조광의 빛에너지는 미백제의 치아미백 효과를 증가시키는 것에 명백한 영향을 주지 않는 것으로 평가되었다.

154kV용 활선 안전 경보기 개발에 관한 연구

정민규,조현광,우종수,안창회,서영석,김응태,박창기,오세득 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

In this report we developed a high voltage alarm for the safety of workers who check and repair the 154kV power line. This high voltage alarm we developed is attached on workers' helmet and alarms automatically in order to let workers know when workers work over the safety distance near 154kV power line. Until now the exact safety distance hasn't been regulated and high voltage alarm we've been using was for the range of 6∼22.9kV power line. We fabricated this high voltage alarm on the basis of electric density and actual measurement of power line. The experimental result was that high voltage alarm at 66kV power line alarmed near 90cm, at 154kV power line alarmed near 160cm from the line. Accordingly, we developed the new high voltage alarm which is operated in the range of 66∼154kV power line.

운동부하 흰쥐에서 포도당 증합체가 심근 당원에 미치는 영향

이석강,조성현,김용운,박덕일,김종연,장용찬 대한스포츠의학회 1994 대한스포츠의학회지 Vol.12 No.1

운동부하전 섭취한 탄수화물이 운동흰쥐의 심근 및 골격근이 당원량에 미치는 영향을 측정하여 심근과 골격근의 대사적 차이점의 일단을 연구한 본 논문의 결과는 다음과 같다. 심근과 골격근 당원량(mg/ g wet tissue, 이하 단위 동일)은 정상군에서 심근 2.62±0.51로서 골격근의 3.37±0.58보다 낮았다(p<0.01). 심근 당원량은 정상군이 2.62±0.51이었다. 운동 30분에서 대조군은 정상군의 63%수준으로 감소하였으며(p<0.01), 탄수화물섭취군은 정상군의 86 및 90%수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 대조군의 142 및 137%정도로 양군 모두 대조군보다 유의하게(p<0.01) 높았다. 운동 60분에는 대조군은 운동30분의 78%(정상군의 49%)수준으로 감소하였으며(p<0.05) 탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 운동 30분에 비하여 72(p<0.01, 정상군의 62%), 81%정도(p<0.05, 정상군의 73%)로 감소하였다. 포도당 섭취군의 당원량은 대조군의 147% 정도로 대조군보다 유의하게(p<0.01) 높았다. 운동 120분에서 대조군은 운동 60분에 비하여 63%(정상군의 33%) 수준으로 감소하였으며 (p<0.01) 포도당섭취군은 운동 60분의 69%(정상군의 50%)수준으로 유의하게 감소하였으나, 포도당 중합체 섭취군은 차이가 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 202 및 162% 정도로 양군 모두 대조군보다 유의하게(p<0.01)높았다. 골격근 당원량은 정상군이 3.37±0.58 이었다. 운동 30분에서 대조군은 정상군의 69% 수준으로 감소하였으며(p<0.01) 탄수화물섭취군은 포도당 중합체 및 포도당섭취군에서 각각 정상군의 85 및 88% 수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 125 및 129% 정도로 양군 모두 대조군보다 유의하게(p<0.05) 높았다. 운동 60분에는 대조군은 운동 30분의 77%(정상군의 53%) 수준으로 감소하였으며(p<0.05)탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 30분에 비하여 93(정상군의 80%), 89(정상군의 78%) %정도로 감소하였으나 통계적으로 유의하지는 않았다. 포도당중합체 및 포도당 섭취군의 당원량은 각각 대조군의 152 및 149% 정도로 대조군보다 유의하게 (p<0.001) 많았다. 운동 120분에서 대조군은 운동 60분에 비하여 50%(정상군의 26%) 수준으로 감소하였으며(p<0.001) 포도당 섭취군은 운동 60분의 75%(정상군의 58%)수준으로 유의하게(p<0.05) 감소하였으나 포도당 중합체 섭취군은 대조군의 84%(정상군의 67%)정도로 감소하는 경향을 보였다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군우ㅢ 255 및 221% 정도로 양군 모두 대조군보다 유의하게(p<0.001) 높았다. 이상의 결과로 미루어 보아 심근과 골격근 간의 생리, 생화학적 특성의 차이에도 불구하고 탄수화물의 섭취후 중등도 정도의 달리기 운동시 근 당원의 함량에 미치는 영향은 정도의 차이는 있으나 같은 경향을 나타내었으며, 골격근에서 심근보다 더 많은 근 당원 절감효과를 나타내었다. 또한 운동전 탄수화물의 섭취시 이러한 근 당원 절감 효과로 장시간의 운동시 심근 및 골격근 당원의 고갈 지연으로 운동수행능력의 증가가 기대되며, 특히 포도당 보다는 포도당 중합체의 섭취시 효과가 더 크리라고 사료된다. The purpose of this study was to investigate the effect of glucose and glucose plymer ingestion on the concentration of the cardiac muscle glycogen at 30, 60 and 12 minutes of the treadmill running in rats. Rats were randomly assinged to normal, control, and carbohydrate groups. Carbohydrate group was divided into glucose, and glucose polymer ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either water (control) of carbohydrates of formulated glucose(6%), and glucose polymer(6%) via polyethylene tube to the stomaach before treadmill run. Exercise was loaded by a treadmill run at the speed of I ㎞/hr for 30, 60, and 120 minutes. The results of experiment were as follows: Glycogen content of the cardiac muscle was lower than that of the skeletal muscle (plantaris) in normal rats. Glycogen content of the cardiac muscle was decreased in proportion to the exercise time compared to normal in control and carbobydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in decreasing order at 120 minutes treadmill running (% change of glycogen content of control, glucose, and glucose polymer compared to normal rats were 33, 50, and 63% in the cardiac muscle at 120 minutes of treadmill running respectively). Glycogen content of the skeletal muscle was decreased in proportion to the exercise time compared to normal in control and corbohydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in ecreasing order 120 minutes treadmill running(% change of glycogen content of control, glucose, and glucose polymer compared to normal were 26, 58, and 67% in the cardiac muscle at 120 minutes of treadmill running respectively). Change of the glycogen content of the cardiac and the skeletal muscle during treadmill running after glucose and glucose polymer ingestion showed that both muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrates ingested rats. Decreasing rate of glycogen content of cardiac and skeletal muscles during treadmill running compared to normal in carbohydrates ingested rats was lower than that of control. From these results, it is suggested glycogen content of cardiac and skeletal muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrate ingested rats. Glycogen content of cardiac and skeletal muscles in carbohydrate singested rats less decreased than that of control during treadmill running compared to normal. Glucose polymer of the carbohydrates ingested rats in considered as the most effective for glycogen sparing according to exercise time in both muscles. Therefore, intake of carbohydrate, especially glucose polymer, before exercise may be increase performance of the prolonged moderate exercise.

두 번의 연속적 뇌경색에 의한 양측성 외측연수증후군 1예

조광덕,이덕희,황정숙,민선호,조아현,조경희 대한노인병학회 2004 대한노인병학회지 Vol.8 No.1

전로제강 슬래그(BOF-Slag)를 이용한 고온탈황에 관한 연구

오광중,정덕영,최성원,조상원,손병현 釜山大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.51 No.-

고온 석탄연료가스의 탈황에 BOF-Slag의 이용가능성을 실험해 보았다. BOR-Slag의 온도, 유량, 탈황제의 특성등이 H₂S 제거효율에 미치는 영향을 조사해 본 결과, 최적의 H₂S 제거효율은 700℃에서 이루어졌고 입자크기가 작을수록 황포집능은 향상되었으나 0.214-0.631mm에서는 큰 영향이 발견되지 않았다. 조업온도 800℃이상에서는 탈황제의 응집으로 내부물질전달저항이 증가하여 탈황제의 반응성이 저하되었다. Experiments have been made to test the practical feasibility of using BOF-Slag to desulfurize hot coal-derived fuel gas. In this study, the effects of particle size, temperature, flow rate and sorbent characteristics on the H₂S removal efficiency of BOF-Slag were investigated. Experimental results showed that the removal efficiency of H₂S was optimum when the temperature was about 700℃ and the smaller sample size, the better sorbent capacity had and the sample size in the range of 0.214∼0.631mm didn't influence. When the temperature was above 800℃, the reactivity of sorbent has lowered because agglomeration of sorbent increased intraparticle transport resistance.

신손상 진단에 있어 현미경적 혈뇨의 의의

임정환,오봉렬,나국주,김현수,윤한덕,허 탁,조석주,민용일 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

The evaluation of patients with microscopic hematuria after blunt abdominal trauma has become controversial. We tested the hypothesis that renal contusion can be diagnosed clinically and that these patients do not require radiographic evaluation. To evaluate the association of microscopic hematuria without shock and with renal injury, we reviewed the medical records of 252 patients following blunt abdominal trauma. Microscopic hematuria without shock was noted in 58 of 252 patients. Of the 58 patients 52 had renal contusion, 4 had renal laceration, 1 had a renal rupture and 1 had renal pedicle injury. All of the patients with renal contusions experienced no complications from nonoperative management. But, avoiding a radiopraphic evaluation in patients with blunt renal trauma plus microscopic hematuria and no shock would miss a few cases of severe renal injury.

벼 상온 저장 시기별 쌀 품질 변화

권석주,송영은,권영립,이덕렬,조승현,최동철 원광대학교 2005 論文集 Vol.35 No.-

Increasing concern about a rice quality, it is considered postharvest technology as well as cultivation management as an important factor. Therefore it is necessary to find optimal drying, storage method that did not affected a rice quality. In order to find the optimum storage period of rice, the rice quality was investigated during storage period. The temperature and humidity of storage bin were greatly changed but those of rice were a little do. The germination rate was slowly decreased as the storage period is long. The content of moisture was affected by a room temperature, it was increased at stored rice in rainy season(10-Jul~20-Jul). As storage period is long, the content of amylose, fatty acidity were a just Little changed but did not showed a constant tendency to it. The content of protein was increased and mechanic palatability value was decreased during storage period. The chromaticity of milled rice showed a range of lightness(L); 75.15~71.75, redness(a); 0.21~0.91, yellowness(b); 16.78~19.28 during storage period.

Prospective Comparison of Intraductal Ultrasonography-Guided Transpapillary Biopsy and Conventional Biopsy on Fluoroscopy in Suspected Malignant Biliary Strictures

( Hyun Su Kim ),( Jong Ho Moon ),( Yun Nah Lee ),( Hyun Jong Choi ),( Hyun Woo Lee ),( Hee Kyung Kim ),( Tae Hoon Lee ),( Moon Han Choi ),( Sang-woo Cha ),( Young Deok Cho ),( Sang-heum Park ) 대한간학회 2018 Gut and Liver Vol.12 No.4

Background/Aims: In suspected malignant biliary strictures (MBSs), the diagnostic yield of endoscopic retrograde cholangiopancreatography (ERCP)-based tissue sampling is limited. Transpapillary forceps biopsy (TPB) under intraductal ultrasonography (IDUS) guidance is expected to improve the diagnostic accuracy in patients with indeterminate biliary strictures. We evaluated the usefulness of IDUS-guided TPB in patients with suspected MBS. Methods: Consecutive patients with suspected MBS were prospectively enrolled in the study. ERCP with IDUS was performed in all patients. Both conventional TPB and IDUS-guided TPB on fluoroscopy were performed in each patient. The primary outcome was the diagnostic accuracy of conventional TPB and IDUS-guided TPB. Results: The technical success rate of IDUS-guided TPB was 97.0% (65/67 patients). Of these 65 patients, the final diagnosis was malignancy in 61 patients (93.8%). On IDUS, the most common finding of IDUS was an intraductal infiltrating lesion in 29 patients (47.5%). The overall diagnostic accuracy was significantly higher using IDUS-guided TPB than that using conventional TPB (90.8% vs 76.9%, p=0.027). According to the subgroup analysis based on the tumor morphology, IDUS-guided TPB had a significantly higher cancer detection rate than conventional TPB for intraductal infiltrating lesions (89.6% vs 65.5%, p=0.028). Conclusions: IDUS-guided TPB appears to improve the accuracy of histological diagnosis in patients with MBS. (Gut Liver 2018;12:463-470)

Reperfusion Injury on Magnetic Resonance Imaging After Carotid Revascularization

Cho, A-Hyun,Cho, Yong-Pil,Lee, Deok Hee,Kwon, Tae-Won,Kwon, Sun U.,Suh, Dae-Chul,Kim, Jong S.,Kang, Dong-Wha American Heart Association, Inc. 2014 Stroke Vol.45 No.2

<P><B>Background and Purpose—</B></P><P>Reperfusion injury can be revealed as delayed gadolinium enhancement in the subarachnoid space on a fluid-attenuated inversion recovery image, which is designated as a hyperintense acute reperfusion marker (HARM). We sought to investigate the occurrence and predictors of HARM and its association with new ischemic infarcts after carotid revascularization.</P><P><B>Methods—</B></P><P>Forty-five patients who underwent carotid endarterectomy or stenting were prospectively enrolled. Diffusion-weighted imaging and fluid-attenuated inversion recovery were performed before and 24 hours after the procedures. Postprocedural fluid-attenuated inversion recovery was done after intravenous gadolinium injection.</P><P><B>Results—</B></P><P>HARM was observed in 8 (17.8%) patients. Of these, 4 patients showed neurological deterioration associated with HARM. These symptomatic HARMs disappeared on follow-up magnetic resonance imaging. Patients with symptomatic HARM were older (<I>P</I>=0.010) and had more frequent leukoaraiosis (<I>P</I>=0.012) and higher postprocedural systolic blood pressure (<I>P</I>=0.025) than those without. New brain infarcts on postprocedural diffusion-weighted imaging were identified in 13 (28.9%) patients. HARM was not associated with new infarcts.</P><P><B>Conclusions—</B></P><P>HARM after carotid revascularization is not uncommon. Symptomatic HARM was associated with old age, underlying leukoaraiosis, and postprocedural high blood pressure.</P>