Study of the effect of diet supplemented with powdered Salicornia herbacea L. on glucose transporter and antioxidant capacities in rats

( Hyo Bin Seo ),( Ju Ock Nam ),( Young Ju Song ),( Tae Dong Kwon ),( Yung Hi Yeo ),( Sung Pil Ryu ) 한국운동영양학회 2012 Physical Activity and Nutrition (Phys Act Nutr) Vol.16 No.2

This study was conducted to examine the possibility of Salicornia herbacea L. powder as a functional food beneficially affecting carbohydrate metabolism and antioxidant capacity. Twenty-four, 6-week old, maleSprague-Dawleyrats were classified into three groups: normal diet control (CON), high-fat diet control (HFC) and high fat diet + Salicornia herbacea L. (SHF). Related feed was provided to each group for 4 weeks. Weight gain rate increased most in the HFC rats, and the concentration of glucose was significantly high in both the HFC and SHF groups, compared to the CON group. The SHF group showed a significantly high expression rate of Glut 4 (21.36%), compared to the CON and HFC groups. The glycogen content in muscle was significantly high in both the HFC and SHF groups, compared to the CON group. There were significant differences in the malondialdehydecontent in muscles between the groups, with the content in the CON and HFC groups being significantly higher than the SHF group. All the groups showed a similar tendency to each other in the liver tissue as well. Concerning the expression of Cu,Zn-super oxide dismutase andglutathione peroxidaseproteins, the SHF group was significantly higher than the CON and HFC groups. Overall, the experiment result above implies a possibility that an intake of Salicornia herbacea L. powder can regulate weight by decreasing the weight gain rate, further suggesting its effectiveness as a functional food before exercise by increasing the energy storage capacity and antioxidant capacity.

Glasswort Powder Diet Activates Lipid Metabolism in Rat

Hyo Bin Seo,Yunyoung Kwak,Ju-Ock Nam,Young Ju Song,Byung-Oh Kim,Sungpil Ryu 한국생명과학회 2012 생명과학회지 Vol.22 No.4

본 연구에서는 함초분 섭취가 지질대사에 미치는 영향을 알아보고자 하였다. 흰쥐를 일반식이 대조군(CON), 고지방식이 대조군(HFC), 고지방식이에 함초분 첨가군(HFG)로 각각 구분하여 4주간 섭취시켰다. 체중은 HFG가 HFC에 비하여 9% 정도 낮았다. 분변칼로리 분석을 한 결과, HFG의 지방량(CON에 비하여 120%, HFC에 비하여 138)과 분변 칼로리(약 110%)가 높은 것으로 나타났다. 혈중 중성지방과 LDL-C는 HFG가 HFC에 비하여 낮았으며(각각 71.8% 및 58%), HDL-C는 HFG가 HFC에 비하여 152% 높았다. 간의 FABP 발현은 HFG가 HFC보다 197% 높았으며, CPT-1 역시 동일한 경향을 보였다. 이러한 결과는 함초분 섭취가 체중의 증가를 억제하였고, 간세포 수준에서의 지방대사를 개선하였다는 점을 시사한다. 이러한 결과를 함초분이 소화관에서의 지방흡수를 억제함으로써 항비만 효과를 가지는 것으로 제안하고자 한다. The purpose of this study was to investigate the effects of glasswort powder intake on lipid metabolism. Rats were divided into three groups: a group fed a normal diet (CON), one fed a high fat diet (HFC), and one fed a high fat diet with glasswort powder (HFG). They were fed their respective diet for four weeks. Body weight was significantly lower (9%) in the HFG group than in the HFC group at the fourth week. According to the feces analysis, the HFG group showed the highest fat level (120% vs. CON; 138% vs. HFC) and fecal calories (110%). The concentration level of TG and LDL-C was 71.8% lower in the HFG group compared to the HFC group, while the concentration level of HDL-C was 152% higher in the HFG group. Expression of FABP in the liver was 197% greater in the HFG compared to the HFC group, with the expression of CPT-1 showing a similar tendency. These results suggest that glasswort powder intake suppresses weight gain and improves fat metabolism at the level of liver cells. From these results, we suggest that glasswort powder is effective against obesity by inhibiting the absorption of fat in the digestive tract.

Orignal Paper : The effect of ginger extract ingestion and swimming exercise on insulin resistance and skeletal muscle antioxidant capacity and apoptosis in hyperglycemic rats fed a high-fructose diet

( Hyo Bin Seo ),( Soo Chun Lee ),( Tae Dong Kwon ),( Jun Yong Kang ),( Young Ju Song ),( Sung Pil Ryu ) 한국운동영양학회 2011 Physical Activity and Nutrition (Phys Act Nutr) Vol.15 No.1

This study purposed to examine the effects of ginger extract and swimming exercise administered to hyperglycemic rats on their insulin resistance, antioxidant ability and apoptosis. For this purpose, we divided 40 15-week-old male SD rats with induced hyperglycemia into control group (CON), exercise group (EX), ginger extract group (GI), and ginger extract + exercise group (GI+EX), and through 8 weeks` experiment we performed weight, blood glucose, insulin concentration, HOMA-IR, SOD activity, TBARS content and apoptosis of gastrocnemius muscle. Body weight was not significantly different among the groups. Blood glucose changed significantly over time in GI and GI-EX. Insulin concentration decreased significantly over time in GI and GI-EX, and was significantly lower in GI-EX among the groups. HOMA-IR decreased significantly over time in EX, GI and GI-EX, and was significantly lower in GI-EX among the groups. Among time points x groups, significant difference was observed in GI-EX at time points 2, 3 and 5. As to the antioxidant ability of gastrocnemius muscle, SOD activity and TBARS content were not changed by ginger extract and exercise, and apoptosis. No significant difference was observed in apoptotic factors P53 and caspase-3, anti-apoptotic factors Bcl-2 among the groups. Accordingly, the administration of ginger extract and exercise are expected to lower blood glucose and prevent diabetes and hyperglycemia, antioxidant capacity and apoptosis in the additional study is necessary.

Acute exercise with Salicornia herbacia L. powder ingestion increases Lipids metabolism in STZ-induced diabetic rats

( Hyo Bin Seo ),( In Ho Lee ),( Byung Duk Jeon ),( Tae Dong Kwon ),( Sung Pil Ryu ) 한국운동영양학회 2012 Journal of exercise nutrition & biochemistry Vol.16 No.4

This study was carried out to investigate the effect of Salicornia herbacea L. (SHL) intake and acute exercise on lipids metabolism in streptozotocin (STZ)-induced diabetic rats. Four different groups of 40 STZ-induced diabetic Sprague-Dawley male rats were given a diet of feed mixed with SHL powder for 6 weeks. To adapt to the acute swimming exercise, the exercise group went through a period of swimming induction over one week. Then, they performed the acute swimming exercise the next day. 24 hours after the acute swimming exercise, the rats were sacrificed and samples were collected and analyzed. There was no significant change in body weight as compared to the control group. Stored fat was found to have decreased in the group which was fed a diet supplemented with SHL powder. There was a significant reduction in blood glucose, triglyceride and total-cholesterol in the SHL intake group only. There was no change in L-FABP and CPT-1 protein expression determined to have occurred due to acute exercise, but there was a statistically significant increase in the expression of both proteins determined for the SHL grouip. Moreover, the SHL group with acute exercise exhibited an increase in cytochrome C oxidase. Based on the results above, it seems that SHL powder could help to prevent various diabetic complications. In addition, it seems necessary to prove the effectiveness of SHL as a healthy functional food by carrying out further research on its intake in relation to metabolic syndromes and exercise capabilities.

Persimmon vinegar ripening with the mountain-cultivated ginseng ingestion reduces blood lipids and lowers inflammatory cytokines in obese adolescents

( Hyo Bin Seo ),( Byung Duk Jeon ),( Sung Pil Ryu ) 한국운동영양학회 2015 Journal of exercise nutrition & biochemistry Vol.19 No.1

[Purpose]This studyinvestigated the effect of the vinegar, which is made of 4-year-old mountain-cultivated ginseng ripened into 4-year-matured persimmonvinegar, on the blood lipids level and inflammatory cytokines concentration in obese female adolescents. [Methods]Subjects ingestedthe vinegar, so-called ``mountain-cultivated ginseng persimmon vinegar (MPV)``, without meals every day for 6 weeks with activitiescontrol. Subjects were grouped into control (CON), persimmon vinegar (PV), and MPV with 10 people in each group. Bloodlipids, triglyceride (TG), total-cholesterol (TC), and high density lipoprotein-cholesterol (HDL-C) were analyzed. Also, glutamicoxaloacetic transaminase (GOT) and glutamate pyruvate transaminase (GPT) were analyzed for the hepatotoxicity. Blood cytokines,interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)were analyzed. [Results]Subjects showed a high reduction in body weight and body fat. Their blood lipid level was effectivelyimproved, and the secretion of inflammatory cytokine was suppressed as well, except for TNF-α. However, the change ratio ofthe cytokines was high in PV and MPV. Such results were similar to those from research subjects who took persimmon vinegaronly (PV), but the effect of the vinegar (MPV) was more remarkable. Besides, this mixture was found to have no effect onthe hepatotoxicity. [Conclusion]The significance of this study is that all the experiments were conducted without controlling researchsubjects`` daily lives, and it is suggested that the vinegar may be recommended as a kind of health supplement food to suppressobesity. Especially, since these two products are traditional foods of Korean people, which have been taken for ages, it is expectedthat the fusing of two foods may be better applied to ordinary people who are concerned about obesity.

Antioxidant and antiapoptotic effects of pine needle powder ingestion and endurance training in high cholesterol-fed rats

( Hyo Bin Seo ),( Nam Ho Lee ),( Sung Pil Ryu ) 한국운동영양학회 2014 Physical Activity and Nutrition (Phys Act Nutr) Vol.18 No.3

Antioxidant and antiapoptotic effects of pine needle powder ingestion and endurancetraining in high cholesterol-fed rats. JENB., Vol. 18, No. 3, pp.301-309, 2014 [Purpose]Pine needle is a kind of medicinal plantingested traditionally for a variety of purposes. Therefore, we examined the antioxidant and antiapoptotic capacities of pine needle ingestion in high cholesterol-fed and endurance exercise-trained rats. [Methods]Animals were divided into six groups as; CON: normal diet control group; EX: normal diet and exercise training group; HC: high cholesterol diet group; HCE: high cholesterol diet and exercise training group; HCP: high cholesterol and pine needle group; HCPE: high-cholesterol and pine needle diet with exercise training group, respectively. Each group consisted of seven Sprague-Dawley male rats. The swim-training groups, EX,HCE, and HCPE swam in the swim pool 60 min/d and 5 d/week for 5 weeks. During the rearing periods, freeze-dried pineneedle powder mix with 5% of the high cholesterol diet was supplied to the HCP and HCPE groups. Gastrocnemius musclewas used as the skeletal muscle. Malondialdehyde (MDA), Mn-containing superoxide dismutase (Mn-SOD), Cu, Zn containing superoxide dismutase (Cu,Zn-SOD), and glutathione peroxidase (GPx) were analyzed for their antioxidant capacities. Finally, p53,Bcl-2 (B-cell lymphoma 2), caspase-3 protein expression was analyzed to determine antiapoptotic ability. [Results]MDA showed low content in HCPE compared to the HC. Mn-SOD, Cu, Zn-SOD, and GPx protein expression was significantly increased by pine needle ingestion and/or exercise training. In addition, suppression of p53 protein expression resulted in Bcl-2 increase followed by caspase-3 decrease with/without pine needle ingestion and exercise training. [Conclusion]When exercise training in addition to pine needle powder ingestion may be a helpful nutritional regimen to athletes and exercisers. [Key word]pine needle, antioxidant, antiapoptosis, endurance exercise, rat.

웨이트사이클링시 산양삼 혼입숙성 감식초 섭취에 의한 지질대사 변화

서효빈 ( Hyo Bin Seo ),김판기 ( Pan Ki Kim ),류승필 ( Sung Pil Ryu ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구에서는 체중의 증량과 감량이 지속되는 웨이트사이클링이 유발되었을 때 4년근 산양삼을 4년간 숙성된 감식초에 혼입하여 숙성된 산양삼 감식초를 쥐에게 경구투여 함으로서 나타나는 지질대사의 변화를 알아보았다. 산양삼 감식초를 필터에 정제하여 2.5배와 5배로 희석한 후 4주간 섭취시켰다. 집단은 일반대조군, 웨이트사이클링 대조군, 산양삼 감식초 2.5배 희석군, 산양삼 감식초 5.0배 희석군으로 구분하였다. 분석은 지질대사 관련 단백질 발현량을 알아보기 위하여 AMPK, PPAR-α, FABP, CPT-1, UCP-2를 분석하였다. 우선 AMPK, PPAR-α, FABP, CPT-1, UCP-2는 일반대조군, 웨이트 사이클링 대조군에 비하여 산양삼 감식초의 희석비율과 관계없이 2.5배, 5.0배 희석군 모두 유의하게 증가하였다. 이러한 결과는 체중의 증량과 감량이 반복적으로 이루어지는 웨이트사이클링이 유발되는 경우에, 산양삼 감식초는 지질대사 증가와 함께 체열생산을 유발하는 단백질의 발현을 효과적으로 증가시켜 항비만식품으로 활용이 가능할 것이라 생각된다. In this study, four-year-old Mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar by oral administration to rats and examined changes in lipid metabolism during weight cycling( Yo-Yo phenomena). The fusion material was filtered and then ingested for 4 weeks with diluted 2.5-fold and 5.0-fold. The normal control group(NCON), weight cycling control group(WCON), 2.5-fold diluted fusion material group(2.5 MPV), 5.0-fold diluted fusion material group(5.0 MPV). In order to examine lipid metabolism-associated proteins, the expression level of AMPK, PPAR-α, FABP, CPT-1, UCP-2 was analyzed. First, AMPK, PPAR-α, FABP, CPT-1, UCP-2 compared with the NCON and WCON, regardless of the dilution ratio of the fusion material, 2.5-fold, 5.0-fold dilution was significantly increased in all groups. These results suggested that four-year-old mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar might be used as anti-obesity food by increasing lipid metabolism during weight cycling.

감식초를 활용한 기능성 음료로서의 혈중 지질 농도 감소와 운동기능성 증대 가능성 검토

서효빈 ( Hyo Bin Seo ),송영주 ( Young Ju Song ),강준용 ( Jun Yong Kang ),권대근 ( Dae Keun Kwon ),김판기 ( Pan Gi Kim ),류승필 ( Sung Pil Ryu ) 한국임학회 2011 한국산림과학회지 Vol.100 No.2

감식초의 섭취가 지질대사와 글리코겐저장 능력향상 및 기능성음료로서의 가능성을 검토하였다. 본 연구는 6주령 SD계 수컷 흰쥐 32마리를 대상으로 CONT(고지방식이 대조군), ACON(고지방식이+증류수 투여), PV2.5(고지방식이+감식초 2.5배 희석), PV5.0(고지방식이+감식초 5배 희석)으로 구분하였다. 각 집단간 체중에는 유의한 차이가 나타나지 않았으나 복강내지방, 고환지방에서는 PV2.5군과 PV5.0군에서 유의하게 낮게 나타났다. 혈액성분변화에서 글루코스는 유의한 차이가 없었으며, TC, LDL-C, TG에서 PV2.5군과 PV5.0군이 유의하게 낮았고, HDL-C은 PV2.5군에서 통계적으로 유의하게 높게 나타났다. 근육과 간의 글리코겐 함량에서는 PV2.5군과 PV5.0군에서 유의하게 높은 저장량을 나타내었다. 이상의 결과 감식초의 섭취는 혈중 지질 억제와 함께 운동시 필요한 글리코겐의 저장능력을 향상시켜 운동수행능력이 증가할 수 있음을 시사하고 있어 다양한 접근이 가능한 기능성 음료서의 가능성을 제시하고 있다. This study was performed to find out the persimmon vinegar as a functional drink on reducing blood lipids and enhancing exercise performance. For these, thirty two Sprague-Dawley male rats were divided into 4 groups; control (CONT), water placebo with high fat diet control (ACON), high fat diet with 2.5 times diluted persimmon vinegar ingestion (PV2.5), and high fat diet with 5.0 times diluted persimmon vinegar ingestion (PV5.0). Body weight was not different. Abdominal fat pads were statistically reduced in PV2.5 and PV5.0 compared to CONT and ACON. Blood glucose was not significant but TC, LDL-C and TG were lower in PV2.5 and PV5.0 than other groups, and HDL-C in PV2.5 was the highest among groups. Glycogen contents in the muscle and liver were higher in PV2.5 and PV5.0 compared to CONT and ACON. These results suggested that persimmon vinegar ingestion may inhibit the blood lipids increase and increase glycogen storage which possibly enhancing exercise performance. Therefore, persimmon vinegar has the possibility as the functional drink.

산양삼 혼입숙성 감식초 섭취에 의한 지질대사 변화

서효빈 ( Hyo Bin Seo ),김판기 ( Pan Ki Kim ),류승필 ( Sung Pil Ryu ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구에서는 4년근 산양삼을 4년간 숙성된 감식초에 혼입하여 숙성된 산양삼 감식초를 쥐에게 경구투여 함으로서 나타나는 지질대사의 변화를 알아보았다. 산양삼 감식초를 필터에 정제하여 2.5배와 5배로 희석한 후 4주간 섭취시켰다. 집단은 대조군, 산양삼 감식초 2.5배 희석군, 산양삼 감식초 5.0배희석군으로 구분하였다. 분석은 지질대사 관련 단백질 발현량을 알아보기 위하여 AMPK, PPAR-α, FABP, CPT-1을 분석하였다. 우선 AMPK, PPAR-α, FABP는 대조군에 비하여 산양삼 감식초의 희석 비율과 관계없이 2.5배, 5.0배 희석군 모두 유의하게 증가하였다. CPT-1은 산양삼 감식초 5.0배 희석군에서 가장 많은 발현량을 보였다. 이러한 결과는 산양삼을 감식초에 혼입숙성한 산양삼 감식초는 지질대사를 증가시켜 비만억제에 효과적으로 사용될 수 있다는 점을 시사한다. In this study, four-year-old Mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar by oral administration to rats and examined changes in lipid metabolism. The fusion material was filtered and then ingested for 4 weeks with diluted 2.5-fold and 5.0-fold. The control group, 2.5-fold diluted fusion material group(2.5 MPV), 5.0-fold diluted fusion material group(5.0 MPV). In order to examine lipid metabolism-associated proteins, the expression level of AMPK, PPAR-α, FABP, CPT-1 was analyzed. First, AMPK, PPAR-α, FABP compared with the control group, regardless of the dilution ratio of the fusion material, 2.5-fold, 5.0-fold dilution was significantly increased in all groups. CPT-1 is 5.0 MPV showed the highest expression levels. These results suggested that four-year-old mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar might be used as anti-obesity food by increasing lipid metabolism.

C2C12 근육모세포와 전기자극에 따른 FNDC5/irisin pathway 기전 연구

서효빈(Seo, Hyo-Bin),전병덕(Jeon, Byung-Duk),권태동(Kwon, Tae-Dong) 한국체육과학회 2023 한국체육과학회지 Vol.32 No.6

The tThe this study, we attempted to investigate the effects of EPS (Electrical Pulse Stimulation) the C2C12 cell line composed of mouse skeletal muscle cells. The experimental group was divided into -Electrical pulses stimulation (CON), Low Electrical pulses stimulation (L-EPS), High Electrical pulses stimulation (H-EPS), electrical impulse was introduced to the differentiated C2C12 myotubes for four days under the condition of high intensity 14V/mm, 50Hz, 1ms, low intensity 0.3 V/mm, 1.0 Hz, and 4.0 ms. Then, the C2C12 cell’s morphological change was analyzed along with changes in the cell length, and western blot analysis was conducted on the protein expressed from the C2C12 cell. The C2C12 cell line, composed of the mouse skeletal muscle cells, was stimulated through EPS after its differentiation. The expression of PPAR-γ was significantly higher in the H-EPS group, and the results of PGC-1α, FNDC5, and irisin showed statistically significant differences between each group, and the L-EPS group treated with electrical stimulation compared to the CON group, the H-EPS group showed significantly higher activity. It is thought that the increase in muscle PGC-1α, FNDC 5, and Irisin, which are browning factors for fat, after electrical stimulation will lead to an increase in UCP-1, PPAR-γ, PRDC 16, and FABP-4 in fat tissue, that is, the intensity of exercise. Accordingly, by presenting positive results in the muscle factor among fat browning factors, based on this, it is possible to establish factors such as increased utilization of energy metabolism, anti-obesity, and metabolic syndrome through research on physiological mechanisms in relation to various exercise intensities and methods.