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글루코스 투여가 운동 중 에너지 기질 이용에 미치는 영향
류승필(Sung Pil Ryu),장응찬(Eung Chan Jang),이상직(Sang Jik Lee),권태동(Tae Dong Kwon),정관우(Kwan Woo Jung),이수천(Soo Chun Lee) 한국운동영양학회 2000 Physical Activity and Nutrition (Phys Act Nutr) Vol.4 No.1
N/A Maintaining blood glucose concentration during exercise is important in the athletic field. Therefore, the purpose of this study was the effects of glucose administration on energy substrates utilization during exercise in the untrained (Utr) and trained (Tr) rats. Rats were administered glucose (1.4g/㎏) into the mouth 1h prior to exercise then exercised on the treadmill speed at 20m/min. They were decapitated at pre, 30min, 60min of exercise, and exhausted. Glucose, lactate, FFA, glycerol, glycogen, and liver triglycerides were measured. Glucose concentration was not significantly changed to the groups. Lactate production, however, was significantly increased during the exercise in the Utr. In addition, FFA was significantly increased during the exercise in the Tr. Glycogen utilization ratio during the exercise in the groups were not significantly different, however, the concentration of before the exercise was significantly higher in the Tr. Moreover, exercise time to exhaustion was shorter in the Utr compared with the Tr. These results suggested that glucose administration 1h prior to exercise was possibly maintained blood glucose concentration, however, training-effect was higher compared with glucose-effect during exercise in the groups.
류승필 ( Sung Pil Ryu ) 한국운동영양학회 2004 Physical Activity and Nutrition (Phys Act Nutr) Vol.8 No.1
In the present study, 5mg/kg BW glucocorticoid was used for developing osteoporosis to the twenty SD male rats. After this treatment, swimming as an exercise therapy to cure osteoporosis was performed for 6 weeks. They ingested commercial diet for the periods of experiment ad-libitum. Abdominal fat accumulation, bone mineral density (BMD), bone mineral content (BMC), and bone broken strength were measured with DEXA and Instron. Bone length and diameter were measured with caliper. Swimming exercise training reduces body weight gain during exercise periods and decreases fat accumulation in epidydimal and mesentary (p<.05). Bone length, diameter, and BMD were not different between groups. However. BMC was increased in swimming exercise group (p<.05). These results suggested that swimming exercise training has the less effect of increasing bone related items as compared as weight bearing exercise training in rats. Therefore, if the more investigate might performed, swimming exercise and other exercise regimen or some supplement has the better effect on the glucocorticoid-induced osteoporosis.
Alendronate 투여와 트레드밀 운동이 골량감소 모델 흰쥐의 골밀도에 미치는 영향
조대승 ( Dae Sung Cho ),이상직 ( San Jik Lee ),류승필 ( Sung Pil Ryu ),김현국 ( Hyun Kook Kim ),장웅찬 ( Eung Chan Jang ),이수천 ( Soo Chun Lee ) 한국운동영양학회 2002 Physical Activity and Nutrition (Phys Act Nutr) Vol.6 No.2
In the present study, 5 ㎎/㎏ BW glucocorticoid (GC) was used for loping osteoporosis. After this treatment, 5 ㎎/㎏ BW alendronate (A) was injected in the abdomen for the medical cure and exercise regimen was performed for weeks. Exercise-training was designed endurance exercise with treadmill exercise (TM), 28 m/min, 30 min/day, 5 day/week. Bone mineral density(BMD), bone mineral content(BMC), and bone intensity were measured with DEXA and Instron. Bone length and diameter were measured with caliper and blood calcium was analyzed with enzymatic method. Osteoporosis induced with GC was higher in BMC. In addition, BMD, intensity, bone length, diameter, and blood calcium was tended to reduce but not significantly different between treatments. GC-A group has increased BMD and blood calcium than other treatments (p<0.05). Diameter and intensity, however, were not significantly different. TM has increased bone length of GC-TM and GC-ATM compared to GC-A (p<0.05), however; diameter was not. These results suggested that only the exercise-training regimen after glucocorticoid-induced osteoporosis is too stressful in rats. Therefore, exercise training combined with alendronate administration is better method to increase those effects.
햄에 혼합한 (-)-Hydroxycitric acid 섭취가 운동 중 에너지 기질 이용에 미치는 영향
임기원 ( Ki Won Lim ),류승필 ( Sung Pil Ryu ),권태동 ( Tae Dong Kwon ),이강우 ( Kang Woo Lee ),서혜정 ( Hea Jung Suh ) 한국운동영양학회 2002 Physical Activity and Nutrition (Phys Act Nutr) Vol.6 No.2
(-)-Hydroxycitric acid(HCA) is known to inhibit increasing malonyl-CoA concentration during endurance. Therefore, we investigated the 5 d administration of HCA mixed in the ham on the cycle ergometer exercise performance athletes. Subjects were administered the 250 ㎎ of HCA (250H), 750㎎ of HCA (750H) and control (Con) for 5 d after the breakfast then were performed cycle ergometer exercise begins with 50 W then increased, progressively. Bloods was collected at rest and every 15 min during exercise and expired gas samples were analyzed at rest and every 5 min at 60% VO_2max. and 80% VO_2max exercise. They exercised on the cycle ergometer at the 60% of their VO_2max for 1 h, and then the exercise intensity was increased at the 80% VO_2max until exhausted. Respiratory exchange ratio of HCA was significantly lower than that of Con (p<0.05). Fat oxidation was significantly increased with administration of HCA and carbohydrate oxidation was significantly decreased (p<0.05) during exercise. Increased fat oxidation has enhanced cycle ergometer exercise time to exhaustion after 1 h of 60% VO_2max exercise in HCA (p<0.05). These results suggested that HCA mixed in the ham enhance endurance performance without interfere fat metabolism, which spares stored glycogen utilization during moderate intensity exercise in athletes.
운동수행과 녹차 플리패늘 섭취가 혈중지질성분과 MDA, SOD에 미치는 영향
권태동 ( Tae Dong Kwon ),류승필 ( Sung Pil Ryu ),장웅찬 ( Eung Chan Jang ),이수천 ( Soo Chun Lee ) 한국운동영양학회 2002 Physical Activity and Nutrition (Phys Act Nutr) Vol.6 No.2
Green tea is widely consumed beverage in the world especially in Asia. It is known to have antioxidative activity and blood lipid lowering effect. In this point of view, green tea extracted polyphenol (50 ㎎/㎏) was administered for 5 wks with treadmill exercise training in experimental rats (EXP; n=8) or water as a placebo (CON; n=8). Blood lipids, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were analyzed and calculated with enzymatic methods. Superoxide dismutase (SOD) and malonedialdehyde (MDA) in liver were analyzed as reported elsewhere. TC and LDL-C, cause of arteriosclerosis, were significantly reduced in FXP (p<0.05). SOD was significantly increased in EXP and MDA was significantly decreased in EXP (p<0.05). These results suggested that green tea polyphenoll decreased the blood lipids and lipid peroxidation by exercise training.
Carnitine과 Hydroxycitrate 복합섭취가 운동 중 지질대사에 미치는 영향
정혁 ( Hyuk Jung ),주만식 ( Man Sik Ju ),류승필 ( Sung Pil Ryu ),이수천 ( Soo Chun Lee ) 한국운동영양학회 2004 Physical Activity and Nutrition (Phys Act Nutr) Vol.8 No.1
Carnitine is essential substance to fatty acid oxidation in mitochondria, where the β-oxidation take place. Hydroxycitrate is the principal acid of fruit of Garcinia cambogia, Garcinia indica, and Garcinia atroviridis. Hydroxycitrate is shown to be a potent inhibitor of ATP lyase, which catalyzes the extra mitochondrial cleavage of citrate to oxaloacetate and acetyl-CoA: citrate + ATP + CoA → actyl-CoA + ADP + Pi + oxaloacetate. The purpose of this study is to examine the effects of carnitine and hydroxycitrate ingestion on lipid metabolism during exercise in rats. 28 male Sprague-Dawley rats were randomly assigned 4 groups (CON: control, HCA: HCA supplementation, CAR: carnitine supplementation, MIX: HCA+carnitine supplementation). All rats that have exercise trained and the diet supplemented in each group for 4weeks. Fat weight, blood glucose, lactate, FFA, TC, TG, HDL-C, LDL-C, Liver and muscle glycogen were analyzed. Total fat weight, TC and LDL-C were significantly decreased in the all experimental group (p<.05). Blood glucose concentration were significantly increased in the HCA and CAR (p<.05). FFA concentration were significantly decreased in the CAR and MIX (p<.05). Glycogen contents were significantly increased MIX (p<.05). These results suggested that 4weeks administration of camitine and hydroxycitrate promotes lipid oxidation and spares carbohydrate utilization during exercise in rat.
권태동(Tea Dong Kwon),최상원(Sang Won Choi),류승필(Sung Pil Ryu),정관우(Kwan Woo Chung),이수천(Soo Chun Lee) 한국운동영양학회 2001 Physical Activity and Nutrition (Phys Act Nutr) Vol.5 No.1
N/A Prolonged endurance exercise training is known to lowering oxidative damage during exercise. Therefore, we examined the effects of endurance exercise training on antioxidants enzymes activities in rats. Rats were trained on the treadmill with 1 % incline, 28m/min, 1h/day, and 5d/wk for 6 wks in the trained group (TR). Sedentary group did not perform the endurance exercise training (NT). They were decapitated at before and after exercise. Then, liver, heart, soleus muscle, gastrocnemius red and white muscle samples were collected for malondialdehyde (MDA) and superoxide dismutase (SOD) analysis. Students t-test was performed as the statistical analysis. Endurance exercise training has significantly reduced MDA concentrations in liver, heart, and gastrocnemius muscle. In addition, SOD concentration was significantly increased in liver, heart, soleus muscle, gastrocnemius red and white muscle. These results suggest that endurance exercise training reduced the risk of lipid peroxidation during exercise in rats.
목초액 단기섭취가 훈련된 흰쥐의 항산화 방어기전에 미치는 영향
이수천,김동기,김재우,서효빈,이윤경,전병덕,이동운,류승필,Lee, Soo-Chun,Kim, Dong-Ki,Kim, Jae-Woo,Seo, Hyo-Bin,Lee, Yoon-Kyung,Jeon, Byung-Duk,Lee, Dong-Woon,Ryu, Sung-Pil 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.4
본 연구는 목초액의 섭취가 훈련된 흰쥐의 항산화방어기전에 미치는 영향을 알아보기 위하여 수행되었다. 실험대상으로는 Sprague-Dawley계 수컷 흰쥐를 사용하였는데 실험그룹은 비운동 대조군과 운동 대조군, 목초액 섭취 비 운동 대조군, 목초액 섭취 운동 대조군으로 나누어 수행하였는데 체중과 혈중성분 변화, 글리코겐 함량, SOD 활성 수준, MDA 함량에 관한 분석을 하였다. 체중과 체지방(장간막, 복막후, 부고환)은 각 그룹 간에 유의한 차이가 없었고, TG와 FFA 농도도 목초액 섭취로 인한 차이는 없었다. Glucose 농도는 대조군 보다 운동군에서 유의한 차이를 나타내었으나 목초액 섭취로 인한 차이는 없었다. 목초액 투여 운동군에서의 SOD 활성은 다른 그룹보다 유의하게 높았다. 운동 대조군에서의 MDA 함량은 비운동 대조군보다 유의하게 낮게 나타났으나 대조군과 목초액 투여군 간의 차이는 없었다. 결론적으로 운동과 목초액 섭취는 산화적 스트레스의 감소에 긍정적인 영향을 줄 것으로 예상된다. The purpose of this study is to know the effect of wood vinegar ingestion on antioxidative defense system in trained rats. Twenty-eight male Sprague-Dawley male rats were used as the subject. Experimental groups were divided into CON-SED(control + sedentary), CON-EX(control + exercise), WV-SED(wood vinegar ingestion + sedentary), and WV-EX(wood vinegar ingestion + exercise), respectively. Body weight and stored fat weight(mesentery, retroperitoneal and epididymal) were not significantly differed in each group. Concentration of TG and FFA was not differed by wood vinegar ingestion. Concentration of glucose was revealed significant difference in exercise group rather than sedentary group, but there was no difference by wood vinegar ingestion between the groups. Contents of SOD in WV-EX group was significantly higher than other three groups. Contents of MDA in CON-EX group was significantly lower than CON-SED group, while the difference between CON group and WV group was not observed. In conclusion, wood vinegar ingestion with exercise seems to have a positive beneficial effects on reducing oxidative stress.