http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yunyoung Kwak,김상준,IN-KOORHEE,신재호 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.6
Quantitative real-time PCR (qPCR) method was applied to quantify the functional gene encoding organophosphorus hydrolase for assessing the degradation efficacy by bacterial strains on an organophosphorus compound in in situ soil. The specific primers targeting the organophosphorus hydrolase were designed and tested on reference bacterial strains and in DNA samples extracted from in situ soil samples contaminated by an organophosphorus compound. The established qPCR assay is a practical method for the analysis of in situ soil samples undergoing bioremediation of organophosphorus compounds.
Kwak, Yunyoung,Rhee, In-Koo,Shin, Jae-Ho Springer-Verlag 2013 Applied microbiology and biotechnology Vol.97 No.18
<P>Concerned with the influence of tagging system on the expression of heterogeneous protein in Escherichia coli, we attempted to express the organophosphorus hydrolase (OPH) of Flavobacterium sp. ATCC 27551 in E. coli. Recombinant OPH was overproduced successfully in E. coli when modified without the use of a tobacco etch virus (TEV) protease cleavage sequence. In addition, though there has never been a report on the extracellular secretion of recombinant OPH harboring native Tat signal peptides in E. coli, the produced protein was observed to be secreted extracellularly. Through the use of reverse transcriptional quantitative real-time PCR and comparison of the predicted folding rate, it was determined that OPH expression may be affected by the existence of a TEV protease cleavage sequence at the C-terminus during the process of translated protein folding, leading to the suppressed OPH activity. With the potential compatibility between native Tat signal peptides of OPH and E. coli Tat pathway secretion system, we report a successful expression of recombinant OPH harboring native Tat signal peptides in E. coli, for the first time.</P>
Kwak, Yunyoung,Khan, Abdur Rahim,Shin, Jae-Ho Elsevier 2015 Journal of biotechnology Vol.193 No.-
<P><B>Abstract</B></P> <P> <I>Serratia nematodiphila</I> DSM 21420<SUP>T</SUP> (=CGMCC 1.6853<SUP>T</SUP>, DZ0503SBS1<SUP>T</SUP>), isolated from the intestine of <I>Heterorhabditidoides chongmingensis</I>, has been known to have symbiotic-pathogenic life cycle, on the multilateral relationships with entomopathogenic nematode and insect pest. In order to better understanding of this rare feature in <I>Serratia</I> species, we present here the genome sequence of <I>S. nematodiphila</I> DSM 21420<SUP>T</SUP> with the significance of first genome sequence in this species.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Serratia nematodiphila</I> DSM 21420<SUP>T</SUP>, isolated from the nematode <I>Heterorhabditidoides chongmingensis</I>, has insect pathogenicity, which near complete genome sequence has been deposited in NCBI. </LI> <LI> The genome sequence of <I>S. nematodiphila</I> DSM 21420<SUP>T</SUP> is the first genome report in the species of <I>S. nematodiphila.</I> </LI> <LI> In the <I>Serratia</I> species, only few cases have been known to occur the insect mortality by harboring the mutualistic relation with entomopathogenic nematode. The genome sequence of <I>S. nematodiphila</I> DSM 21420<SUP>T</SUP> can support to reveal the mechanisms of unknown regulatory systems for these rare features. </LI> </UL> </P>
Kwak, Yunyoung,Kim, Sang-Jun,Rhee, In-Koo,Shin, Jae-Ho 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.6
Quantitative real-time PCR (qPCR) method was applied to quantify the functional gene encoding organophosphorus hydrolase for assessing the degradation efficacy by bacterial strains on an organophosphorus compound in in situ soil. The specific primers targeting the organophosphorus hydrolase were designed and tested on reference bacterial strains and in DNA samples extracted from in situ soil samples contaminated by an organophosphorus compound. The established qPCR assay is a practical method for the analysis of in situ soil samples undergoing bioremediation of organophosphorus compounds.
Kwak, Yunyoung,Jung, Byung Kwon,Shin, Jae-Ho Elsevier 2015 Journal of biotechnology Vol.193 No.-
<P><B>Abstract</B></P> <P> <I>Pseudomonas rhizosphaerae</I> IH5<SUP>T</SUP> (=DSM 16299<SUP>T</SUP>), isolated from the rhizospheric soil of grass growing in Spain, has been reported as a novel species of the genus <I>Pseudomonas</I> harboring insoluble phosphorus solubilizing activity. To understanding the multifunctional biofertilizer better, we report the complete genome sequence of <I>P. rhizosphaerae</I> IH5<SUP>T</SUP>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>P. rhizosphaerae</I> IH5<SUP>T</SUP> is a novel bacterium having phosphorus solubilizing activity. </LI> <LI> Complete genome sequence of strain IH5<SUP>T</SUP> is the first genome report in this species. </LI> <LI> Genome sequence of strain IH5<SUP>T</SUP> provides biological bases for the multifunctional biofertilizer. </LI> </UL> </P>
Glasswort Powder Diet Activates Lipid Metabolism in Rat
Hyo Bin Seo,Yunyoung Kwak,Ju-Ock Nam,Young Ju Song,Byung-Oh Kim,Sungpil Ryu 한국생명과학회 2012 생명과학회지 Vol.22 No.4
본 연구에서는 함초분 섭취가 지질대사에 미치는 영향을 알아보고자 하였다. 흰쥐를 일반식이 대조군(CON), 고지방식이 대조군(HFC), 고지방식이에 함초분 첨가군(HFG)로 각각 구분하여 4주간 섭취시켰다. 체중은 HFG가 HFC에 비하여 9% 정도 낮았다. 분변칼로리 분석을 한 결과, HFG의 지방량(CON에 비하여 120%, HFC에 비하여 138)과 분변 칼로리(약 110%)가 높은 것으로 나타났다. 혈중 중성지방과 LDL-C는 HFG가 HFC에 비하여 낮았으며(각각 71.8% 및 58%), HDL-C는 HFG가 HFC에 비하여 152% 높았다. 간의 FABP 발현은 HFG가 HFC보다 197% 높았으며, CPT-1 역시 동일한 경향을 보였다. 이러한 결과는 함초분 섭취가 체중의 증가를 억제하였고, 간세포 수준에서의 지방대사를 개선하였다는 점을 시사한다. 이러한 결과를 함초분이 소화관에서의 지방흡수를 억제함으로써 항비만 효과를 가지는 것으로 제안하고자 한다. The purpose of this study was to investigate the effects of glasswort powder intake on lipid metabolism. Rats were divided into three groups: a group fed a normal diet (CON), one fed a high fat diet (HFC), and one fed a high fat diet with glasswort powder (HFG). They were fed their respective diet for four weeks. Body weight was significantly lower (9%) in the HFG group than in the HFC group at the fourth week. According to the feces analysis, the HFG group showed the highest fat level (120% vs. CON; 138% vs. HFC) and fecal calories (110%). The concentration level of TG and LDL-C was 71.8% lower in the HFG group compared to the HFC group, while the concentration level of HDL-C was 152% higher in the HFG group. Expression of FABP in the liver was 197% greater in the HFG compared to the HFC group, with the expression of CPT-1 showing a similar tendency. These results suggest that glasswort powder intake suppresses weight gain and improves fat metabolism at the level of liver cells. From these results, we suggest that glasswort powder is effective against obesity by inhibiting the absorption of fat in the digestive tract.