솔잎 분말 첨가 섭취와 수영훈련이 에너지 대사 기질에 미치는 영향

서효빈(Hyobin Seo),구본문(Bon-Moon Koo),최석립(Seokrip Choi),김우철(Woocheol Kim),류승필(Sungpil Ryu) 한국생활환경학회 2015 한국생활환경학회지 Vol.22 No.2

The purpose of this study is to observe the effects of pine needles powder intake and athletic training on the energy metabolic substrates. To achieve this purpose, 42 Sprague-Dawley male rats were selected to feed pine needles powder and swimming exercise for 5 weeks. There was no significant difference in the weight increase rate while intaking pine needles powder and exercising at the same time. Compared to the swimming exercise group, high cholesterol diet group and high cholesterol diet+swimming exercise group, the pine needles powder intaking group and pine needles powder intake+swimming exercise group suppressed AMPK expression, significantly. Thus, for the result of GLUT-2 and GLUT-4 which are the glucose transporter of liver and skeletal muscle, the pine needles powder intaking group and pine needles powder intake+swimming exercise group showed significantly higher expression. For the glycogen content of liver and muscle, the pine needles powder intaking group and pine needles powder intake+swimming exercise group increased significantly. Therefore, the findings of this study would be used as the basal data of exercise nutrient supplements as the pine needles powder intake gives positive effect on the energy storage capacity.

인삼녹엽 추출물 투여와 자발적 운동 수행에 대한 에너지대사 연구

서효빈(Seo, Hyo-Bin),남주옥(Nam, Ju-Ock),전병덕(Jeon, Byung-Duk),최승식(Choi, Seung-Sig),권태동(Kwon, Tae-Dong) 한국체육과학회 2018 한국체육과학회지 Vol.27 No.3

The purpose of this study is to observe the effect of ginseng green leaf extract and voluntary exercise performance on metabolic substrate. To achieve this purpose, 28 male mice were selected to ingestion ginseng green leaf extract and let voluntary exercise for 4 weeks. Compared to normal diet control group body weight gain ratio was significantly decreased in the voluntary exercise group, ginseng green leaf extracts ingestion group, ginseng green leaf extracts ingestion and voluntary exercise group. For the change of AMPK, AKT, Glut2, Glut4 capacity, AMPK, AKT showed significantly high activeness in the ginseng green leaf extracts ingestion, ginseng green leaf extracts ingestion and voluntary exercise group and Glut2 in the ginseng green leaf extracts ingestion and voluntary exercise group. Compared to each group Glut4 express activity was significant. Therefore, the result showed that specific medicinal ingredients which the ginseng green leaf extracts ingestion worked, proposing positive result for the functionality of ginseng green leafs. In this finding, Tstudy would be of help to provide basic data of exercise supplements as the ginseng green leaf extract ingestion gives positive effect on the energy storage capacity.

청국장 분말 섭취와 운동이 혈중 지질농도 및 SOD활성과 MDA함량에 미치는 영향

서효빈(Seo Hyo-Bin),김현국(Kim Hyun-Kuk) 한국체육과학회 2008 한국체육과학회지 Vol.17 No.4

With the reported fact that chungkukjang(CKJ), made from fermented beans, has thrombolysis ability, suppression of blood pressure increase, anti-mutability, anti-cancer and anti-tumor action, and also the bean-fermented food has high anti-oxidative activity. In there point of views, chungkukjang power was used as the supplement itself. Thirty two rats were divided into normal(CON) normal+exercise(CON-EX), chungkukjang(CKJ), chungkukjang+exercise(CKJ-EX) were sub-group, respectively. Effect of exercise performance and CKJ diet intake for five weeks on improvement of blood lipid and SOD, MDA of male rats. Body weight has decreased with lowered total fat amount in both CKJ diet and exercise group, and CKJ diet intake resulted in significanttly TG concentration but did not have significance on TC, HDL-C, LDL-C, SOD activity in liver was significantly increased in both CKJ diet and exercise group, but they did not have significant effect on MDA amount in liver and SOD activity and MDA amount in gastrocnemius muscle. It is concluded, therefore, that having both CKJ intake and exercise for a long period has positive effect on blood lipid and SOD, MDA.

지구성 운동전 감식초 섭취시 에너지기질의 이용

서효빈 ( Hyobin Seo ),남주옥 ( Ju Ock Nam ),전병덕 ( Byung Duk Jeon ),김판기 ( Pan Gi Kim ),유승필 ( Sungpil Ryu ) 한국산림과학회 2012 한국산림과학회지 Vol.101 No.4

The purpose of this study was to investigate the potential of persimmon vinegar as a functional beverage by analyzing the effects of persimmon vinegar ingestion on the energy substrate during endurance exercise. The healthy male adolescents (n=8) drunk persimmon vinegar ingested trial (PSV) or purified water ingested trial as the control trial (CON) 1 h prior to the exercise with the 60% of maximal oxygen uptake (VO_₂max) for 1 h. The exercise intensity was increased to the 80% of VO₂_max and remained until exhaustion. And the physiological variables blood components and amounts of energy oxidation were analyzed. There was no significant difference between trials in physiological variables and the heart rates after exhaustion were higher in PSV compared to CON. There was no significant difference between trials in blood glucose level while the blood lactic acids decreased significantly in PSV 30 and 60 minutes after onset of exercise. The free fatty acids concentration increased significantly in PSV from 15 minutes to 60 minutes after onset of exercise. The carbohydrate oxidation decreased significantly in PSV from 45 minutes after exercise and on the contrary the fatty acids oxidation increased significantly for the same period. And fatty acids oxidation was higher in PSV compared to C|ON even after exhaustion. The respiratory exchange ratio was lower significantly in PSV compared to CON from 30 minutes to 60 minutes after exercise whereas lower in CON after total exhaustion. The exercise time to exhaustion was 41% longer in PSV compared to CON. These results showed that the persimmon vinegar increase the level of lipids metabolism and decrease sense of fatigue by inhibiting carbohydrate oxidation during moderate intensity exercise suggesting the possibility of using of persimmon vinegar as exercise functional beverage when ingested 1 h prior to the endurance exercise performance.

산양삼 첨가 고지방식이가 골격근의 항세포사멸에 대한 작용 연구

서효빈 ( Hyobin Seo ),김판기 ( Pan Gi Kim ),류승필 ( Sungpil Ryu ) 한국산림과학회 2015 한국산림과학회지 Vol.104 No.3

The purpose of this study was to investigate the anti-apoptosis-related protein expression in skeletal muscle of rats with different amount of mountain ginseng (MG) added high-fat diet fed. Twenty-four Sprague Dawley male rats were divided into the high-fat diet control group (CON), 0.5% of MG added diet group (MG1), and 1.0% of MG added diet group (MG2) with eight rats each. The P53, anti-apoptotic protein, was significantly lower in MG2 than CON and MG1. The bcl-2 and bcl-xl, however, were not significantly different from MG1 but from CON. The caspase-9 and -3, were significantly lower in MG groups than CON. In addition, it was dramatically lower in MG2. These results suggested that MG addition to the high-fat diet suppressed p53 protein expression and enhanced anti-apoptototic protein expression. MG may be a positive effects on health as a medicinal plant.

C2C12 근육모세포와 전기자극에 따른 FNDC5/irisin pathway 기전 연구

서효빈(Seo, Hyo-Bin),전병덕(Jeon, Byung-Duk),권태동(Kwon, Tae-Dong) 한국체육과학회 2023 한국체육과학회지 Vol.32 No.6

The tThe this study, we attempted to investigate the effects of EPS (Electrical Pulse Stimulation) the C2C12 cell line composed of mouse skeletal muscle cells. The experimental group was divided into -Electrical pulses stimulation (CON), Low Electrical pulses stimulation (L-EPS), High Electrical pulses stimulation (H-EPS), electrical impulse was introduced to the differentiated C2C12 myotubes for four days under the condition of high intensity 14V/mm, 50Hz, 1ms, low intensity 0.3 V/mm, 1.0 Hz, and 4.0 ms. Then, the C2C12 cell’s morphological change was analyzed along with changes in the cell length, and western blot analysis was conducted on the protein expressed from the C2C12 cell. The C2C12 cell line, composed of the mouse skeletal muscle cells, was stimulated through EPS after its differentiation. The expression of PPAR-γ was significantly higher in the H-EPS group, and the results of PGC-1α, FNDC5, and irisin showed statistically significant differences between each group, and the L-EPS group treated with electrical stimulation compared to the CON group, the H-EPS group showed significantly higher activity. It is thought that the increase in muscle PGC-1α, FNDC 5, and Irisin, which are browning factors for fat, after electrical stimulation will lead to an increase in UCP-1, PPAR-γ, PRDC 16, and FABP-4 in fat tissue, that is, the intensity of exercise. Accordingly, by presenting positive results in the muscle factor among fat browning factors, based on this, it is possible to establish factors such as increased utilization of energy metabolism, anti-obesity, and metabolic syndrome through research on physiological mechanisms in relation to various exercise intensities and methods.

감식초를 활용한 기능성 음료로서의 혈중 지질 농도 감소와 운동기능성 증대 가능성 검토

서효빈 ( Hyo Bin Seo ),송영주 ( Young Ju Song ),강준용 ( Jun Yong Kang ),권대근 ( Dae Keun Kwon ),김판기 ( Pan Gi Kim ),류승필 ( Sung Pil Ryu ) 한국임학회 2011 한국산림과학회지 Vol.100 No.2

감식초의 섭취가 지질대사와 글리코겐저장 능력향상 및 기능성음료로서의 가능성을 검토하였다. 본 연구는 6주령 SD계 수컷 흰쥐 32마리를 대상으로 CONT(고지방식이 대조군), ACON(고지방식이+증류수 투여), PV2.5(고지방식이+감식초 2.5배 희석), PV5.0(고지방식이+감식초 5배 희석)으로 구분하였다. 각 집단간 체중에는 유의한 차이가 나타나지 않았으나 복강내지방, 고환지방에서는 PV2.5군과 PV5.0군에서 유의하게 낮게 나타났다. 혈액성분변화에서 글루코스는 유의한 차이가 없었으며, TC, LDL-C, TG에서 PV2.5군과 PV5.0군이 유의하게 낮았고, HDL-C은 PV2.5군에서 통계적으로 유의하게 높게 나타났다. 근육과 간의 글리코겐 함량에서는 PV2.5군과 PV5.0군에서 유의하게 높은 저장량을 나타내었다. 이상의 결과 감식초의 섭취는 혈중 지질 억제와 함께 운동시 필요한 글리코겐의 저장능력을 향상시켜 운동수행능력이 증가할 수 있음을 시사하고 있어 다양한 접근이 가능한 기능성 음료서의 가능성을 제시하고 있다. This study was performed to find out the persimmon vinegar as a functional drink on reducing blood lipids and enhancing exercise performance. For these, thirty two Sprague-Dawley male rats were divided into 4 groups; control (CONT), water placebo with high fat diet control (ACON), high fat diet with 2.5 times diluted persimmon vinegar ingestion (PV2.5), and high fat diet with 5.0 times diluted persimmon vinegar ingestion (PV5.0). Body weight was not different. Abdominal fat pads were statistically reduced in PV2.5 and PV5.0 compared to CONT and ACON. Blood glucose was not significant but TC, LDL-C and TG were lower in PV2.5 and PV5.0 than other groups, and HDL-C in PV2.5 was the highest among groups. Glycogen contents in the muscle and liver were higher in PV2.5 and PV5.0 compared to CONT and ACON. These results suggested that persimmon vinegar ingestion may inhibit the blood lipids increase and increase glycogen storage which possibly enhancing exercise performance. Therefore, persimmon vinegar has the possibility as the functional drink.

산양삼 혼입숙성 감식초 섭취에 의한 지질대사 변화

서효빈 ( Hyo Bin Seo ),김판기 ( Pan Ki Kim ),류승필 ( Sung Pil Ryu ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구에서는 4년근 산양삼을 4년간 숙성된 감식초에 혼입하여 숙성된 산양삼 감식초를 쥐에게 경구투여 함으로서 나타나는 지질대사의 변화를 알아보았다. 산양삼 감식초를 필터에 정제하여 2.5배와 5배로 희석한 후 4주간 섭취시켰다. 집단은 대조군, 산양삼 감식초 2.5배 희석군, 산양삼 감식초 5.0배희석군으로 구분하였다. 분석은 지질대사 관련 단백질 발현량을 알아보기 위하여 AMPK, PPAR-α, FABP, CPT-1을 분석하였다. 우선 AMPK, PPAR-α, FABP는 대조군에 비하여 산양삼 감식초의 희석 비율과 관계없이 2.5배, 5.0배 희석군 모두 유의하게 증가하였다. CPT-1은 산양삼 감식초 5.0배 희석군에서 가장 많은 발현량을 보였다. 이러한 결과는 산양삼을 감식초에 혼입숙성한 산양삼 감식초는 지질대사를 증가시켜 비만억제에 효과적으로 사용될 수 있다는 점을 시사한다. In this study, four-year-old Mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar by oral administration to rats and examined changes in lipid metabolism. The fusion material was filtered and then ingested for 4 weeks with diluted 2.5-fold and 5.0-fold. The control group, 2.5-fold diluted fusion material group(2.5 MPV), 5.0-fold diluted fusion material group(5.0 MPV). In order to examine lipid metabolism-associated proteins, the expression level of AMPK, PPAR-α, FABP, CPT-1 was analyzed. First, AMPK, PPAR-α, FABP compared with the control group, regardless of the dilution ratio of the fusion material, 2.5-fold, 5.0-fold dilution was significantly increased in all groups. CPT-1 is 5.0 MPV showed the highest expression levels. These results suggested that four-year-old mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar might be used as anti-obesity food by increasing lipid metabolism.

웨이트사이클링시 산양삼 혼입숙성 감식초 섭취에 의한 지질대사 변화

서효빈 ( Hyo Bin Seo ),김판기 ( Pan Ki Kim ),류승필 ( Sung Pil Ryu ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 연구에서는 체중의 증량과 감량이 지속되는 웨이트사이클링이 유발되었을 때 4년근 산양삼을 4년간 숙성된 감식초에 혼입하여 숙성된 산양삼 감식초를 쥐에게 경구투여 함으로서 나타나는 지질대사의 변화를 알아보았다. 산양삼 감식초를 필터에 정제하여 2.5배와 5배로 희석한 후 4주간 섭취시켰다. 집단은 일반대조군, 웨이트사이클링 대조군, 산양삼 감식초 2.5배 희석군, 산양삼 감식초 5.0배 희석군으로 구분하였다. 분석은 지질대사 관련 단백질 발현량을 알아보기 위하여 AMPK, PPAR-α, FABP, CPT-1, UCP-2를 분석하였다. 우선 AMPK, PPAR-α, FABP, CPT-1, UCP-2는 일반대조군, 웨이트 사이클링 대조군에 비하여 산양삼 감식초의 희석비율과 관계없이 2.5배, 5.0배 희석군 모두 유의하게 증가하였다. 이러한 결과는 체중의 증량과 감량이 반복적으로 이루어지는 웨이트사이클링이 유발되는 경우에, 산양삼 감식초는 지질대사 증가와 함께 체열생산을 유발하는 단백질의 발현을 효과적으로 증가시켜 항비만식품으로 활용이 가능할 것이라 생각된다. In this study, four-year-old Mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar by oral administration to rats and examined changes in lipid metabolism during weight cycling( Yo-Yo phenomena). The fusion material was filtered and then ingested for 4 weeks with diluted 2.5-fold and 5.0-fold. The normal control group(NCON), weight cycling control group(WCON), 2.5-fold diluted fusion material group(2.5 MPV), 5.0-fold diluted fusion material group(5.0 MPV). In order to examine lipid metabolism-associated proteins, the expression level of AMPK, PPAR-α, FABP, CPT-1, UCP-2 was analyzed. First, AMPK, PPAR-α, FABP, CPT-1, UCP-2 compared with the NCON and WCON, regardless of the dilution ratio of the fusion material, 2.5-fold, 5.0-fold dilution was significantly increased in all groups. These results suggested that four-year-old mountain-cultivated ginseng ripened into four-year-matured persimmon vinegar might be used as anti-obesity food by increasing lipid metabolism during weight cycling.

인삼녹엽 추출물 투여와 자발적 운동이 염증성 사이토카인 및 항세포사멸인자 발현에 미치는 영향

황인태(Hwang, In-Tae),서효빈(Seo, Hyo-Bin),남주옥(Nam, Ju-Ock),전병덕(Jeon, Byung-Duk),최승식(Choi, Seung-Sig),권태동(Kwon, Tae-Dong) 한국체육과학회 2019 한국체육과학회지 Vol.28 No.3

The purpose of this study is to observe the effect of ginseng green leaf extract and voluntary exercise on inflammatory cytokine and anti-apoptosis expresses. To achieve this purpose, 28 male rats were selected to ingestion ginseng green leaf extract and let voluntary exercise for 4 weeks. Compared to normal diet control group, ginseng green leaf extracts ingestion group, voluntary exercise group, ginseng green leaf extracts ingestion and voluntary exercise group TNF-α concentration was no showed significantly, NF-κB concentration respectively group was no showed significantly. For the change of apoptosis, p53 no showed significantly and Bcl-2 showed significantly high activeness in the ginseng green leaf extracts ingestion and voluntary exercise group and cleaved caspase-3/caspase-3 was significantly decreased in the control group, ginseng green leaf extracts ingestion and voluntary exercise group significantly decreased . Therefore, the results suggest that immune function and apoptosis is improved by ginseng green leaf extract ingestion and/or exercise training that may inhibit the anti-inflammation and anti-apoptosis process effectively increasing antioxidant capacity in rats. In this finding, various researches on the physiological agents which the ginseng green leafs possesses would give positive effect as a exercise functional food.