Systemic administration of low dosage of tetanus toxin decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus

Bing Chun Yan,In Hye Kim,Joon Ha Park,Ji Hyeon Ahn,Jeong-Hwi Cho,Bai Hui Chen,Jae-Chul Lee,Jung Hoon Choi,Ki-Yeon Yoo,Choong Hyun Lee,Jun Hwi Cho,Jong-Dai Kim,Moo-Ho Won 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.3

In the present study, we investigated the effect of Tetaus toxin (TeT) on cell proliferation and neuroblast differentiation using specific markers: 5-bromo-2-deoxyuridine (BrdU) as an exogenous marker for cell proliferation, Ki-67 as an endogenous marker for cell proliferation and doublecortin (DCX) as a marker for neuroblasts in the mouse hippocampal dentate gyrus (DG) after TeT treatment. Mice were intraperitoneally administered 2.5 and 10 ng/kg TeT and sacrificed 15 days after the treatment. In both the TeT-treated groups, no neuronal death occurred in any layers of the DG using neuronal nuclei (NeuN, a neuron nuclei maker) and Fluoro-Jade B (F-J B, a high-affinity fluorescent marker for the localization of neuronal degeneration). In addition, no significant change in glial activation in both the 2.5 and 10 ng/kg TeT-treated-groups was found by GFAP (a marker for astrocytes) and Iba-1 (a marker for microglia) immunohistochemistry. However, in the 2.5 ng/kg TeT-treated-group, the mean number of BrdU, Ki-67 and DCX immunoreactive cells, respectively, were apparently decreased compared to the control group, and the mean number of each in the 10 ng/kg TeT-treated-group was much more decreased. In addition, processes of DCX-immunoreactive cells, which projected into the molecular layer, were short compared to those in the control group. In brief, our present results show that low dosage (10 ng/kg) TeT treatment apparently decreased cell proliferation and neuroblast differentiation in the mouse hippocampal DG without distinct gliosis as well as any loss of adult neurons.

과학 포트폴리오 체제의 적용이 초등학생의 사회심리학적 교실 환경에 미치는 효과

조선형,김찬종,김범기,김철영,김혜정 한국교원대학교 과학교육연구소 2001 청람과학교육연구논총 Vol.11 No.1

The purpose of the research is to investigate the effects of a portfolio system on students' socio-psychological classroom environments in elementary science class. Two schools were selected from a metropolitan area, a city, and one school from a rural area in central Korea. Two fifth-grade classes were sampled from each school selected, and one class is assigned to experimental group and the other to control group, respectively. The total number of participants of the study is 399. Portfolio system was administered to experimental group, and conventional teaching to control group for two and half month. WIHIC, an inventory to measure socio-psychological classroom environment, was administered as a pre-and post-test. Experimental group gained statistically higher scores on WIHIC after administering portfolio system. They showed more positive perceptions on sub-areas of WIHIC such as teacher support, task orientation, and equity. There is interaction between region and group: the effects of the portfolio system is highest in rural area, lowest in metropolitan area, and the city in between. Portfolio system have positive effects on students' socio-psychological classroom environments. Enhanced socio-psychological classroom environments is expected to contribute to positive self-concept, higher science achievements, and self-directed learning.

Clostridium difficile 장염에 의한 속발성 유전분증에서 발현된 독성 장염

조형원,정혜경,강현주,이윤표,강혜원,심기남,정성애 Ewha Womans University School of Medicine 2014 EMJ (Ewha medical journal) Vol.37 No.1

Amyloidosis is characterized by extracellular deposition of protein fibrils in one or multiple organs. AA amyloidosis is secondarily occurred to be related with chronic infections or inflammatory diseases. We report a 67-year-old man suffered from secondary AA amyloidosis related with chronic Clostridium difficile colitis after repeated total hip replacement surgery. Infection control is the most important treatment of AA amyloidosis secondary to chronic infection. However, the patient’s C. difficile colitis was not controlled well, eventually toxic megacolon with sepsis was developed. Consequently, he had to take total colectomy, but he expired with multi-organ failures. We suggested that early surgical procedure might be one option for intractable C. difficile colitis complicated with secondary amyloidosis.

교통사고에 의해 발생한 뇌내 좌상 1례

박혜연,전소분,김선아,정금선,조종기,송근호,이영원 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A two years old intact female Minature Schnauzer was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with an intermittent seizure by traffic accident. No specific finding was found by radiography and computer tomography (CT). Hematologic and blood chemical examination revealed that PCV (62.5%), BUN (72.6 mg/dl), CK (1769 U/l), ALT (169 U/l) and ALP (353 U/l) were increased. This dog was recovered by medical treatment.

원예치료 및 미술치료가 학교 부적응 청소년의 자아개념 향상에 미치는 영향연구

손기철,엄수진,배혜진,조문경,은옥주,최외선 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

본 연구는 원예치료와 미술치료가 학교부적응 청소년의 자아개념, 내적 통제력, 불안감소에 미치는 영향을 조사하고자 실시되었으며, 그 결과는 각 치료의 효과성이 비교·분석되었다. 인천 남구에 위치한 W공업고등학교에서 대조군 15명, 원예치료군 15명, 미술치료군 15명을 주 2회씩 2001년 9월 27일부터 12월 13일까지 원예치료군에는 원예치료 프로그램을 미술치료군에는 미술치료프로그램을 실시하였다. 원예치료 및 미술치료는 각각 전문 치료사에 의해서 행해졌다. 원예치료 및 미술치료 프로그램의 효과를 알아보고자 자아개념(Self-Concept Scale), 내적 통제력 척도(Inner-Control Scale), 특성불안 척도(Trait-Anxiety Scale)을 각 프로그램의 실시전과 실시후로 조사하여 비교하였다. 원예치료군에 있어서는 자아개념척도 중 자아수용, 성격적 자아, 총 자아 긍정 항목에서 통계적으로 유의한 향상을 보였고, 특성불안척도에서는 우울 및 불안 항목에서 매우 유의한 감소를 나타내었다. 한편, 미술치료군에 있어서는 내적통제력과 특성불안척도중 자신감이 유의하게 향상된 것으로 나타났다. 이상의 결과를 종합하여 볼 때, 원예치료군은 프로그램 과정중 자기표현의 기회를 제공함으로써 자아개념이 향상되었으며, 또한, 공동작업을 하는 집단 프로그램을 통하여 내면의 불안과 갈등이 완화되었다고 판단된다. 반면, 미술치료군은 치료자와의 개인적인 상담과정을 통하여 내면의 불안과 갈등이 완화되었고, 지점토 및 칼라믹스 만들기 등 활동적인 프로그램을 통하여 내면의 감정을 통제하는 능력이 향상되었다고 판단된다. This study was conducted to evaluate the effect of HT (horticultural therapy) and AT (art therapy) on the changes of self-concept, inner control, trait anxiety of the school maladjusted adolescents. 45 school maladaptive of teenage students participated in therapy program at the W high school were randomly assigned to 3 groups: 15 for the horticultural therapy, 15 for art therapy, 15 for control group. The program were carried out from Sep. 27 to Dec. 13, 2001 as an activity group. Activity group attended twice a week for 1 hour. For evaluation of the effectiveness of each therapy, Self-Concept Scale, Inner-Control Scale, Trait-anxiety Scale were used 2 times, that is, before/after HT or AT program. According to the results, self satisfiction, personal self, and total positive self items among Self-Concept Scale were significantly improved, and tension and anxiety item among Trait anxiety Scale were significantly decreased in HT group. On the other hand, in AT group, both inner control and self confidence item in Trait-anxiety Scale were significantly improved. In conclusion, in HT group it was suggested that the improvement of self concept could be brought about by being provided the chance of self expression during program and the alleviation of inner anxiety and discord resulted from group therapy program in which joint work have been provided. In case of AT group, it was logically postulated that the alleviation of inner anxiety and discord resulted from personal counselling with AT therapist during program and the improvement of inner control was caused by active program such as painting paper clay, making color mix, and etc.

만성 호중구성 백혈병 1예

박기령,조성민,우가은,이기현,손혜영,임정윤,최진혁,이순남,정화순 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.2

Chronic neutrophilic leukemia(CNL) is a very rare myeloproliferative disease, characterized by sustained mature neutrophilic leukocytosis with granulocytic bone marrow infiltration, high NAP(neutrophilic alkaline phosphatase) score and absence of philadelphia chromosome, It is frequently accompanied by hepatosplenomegaly, elevated serum vitamin B_12 and uric acid level. For the diagnosis of CNL, the leukemoid reaction, especially secondary to neoplasia, infection and autoimmune diseases, should be excluded. Since Tuohy's first description in 1920, more than 50 cases fullfilling the above criteria have been reported worldwide, and 4 cases in Korea. Several authors have demonstrated the defect of intracellualr killing in the mature neutrophil and this finding correlates well with the very high incidence of fatal infection, The hemorrhagic diasthesis in CNL is caused by functional abnormality of the platelet. This disease has tendency to transform to blastic crisis and acute leukemia as in other myeloproliferative disease, but characteristically shows frequent coexistence with multiple myeloma. Until now , the therapeutic trials in CNL have been disappointing. Hydroxyrea and busulfan can control hyperleukocytosis. On the basis of functional defect in neutrophil, alpha-2b-interferon has been tried and several reports have demonstrated the clinical and functional effect of interferon on CNL. CNL is very rate hematologic disease and there are few report about general aspect of disorder. We report here a typical CNL case presenting with splenomegaly and leukocytosis with a review of the literature.

수종의 dentin bonding agent가 도재라미네이트용 레진시멘트의 전단 결합강도 및 변연 누출에 미치는 영향

박윤기,조혜원,권주홍 大韓齒科器材學會 1993 대한치과재료학회지 Vol.20 No.1

The purpose of this study was to evaluate the influence of three dentin bonding agents on the shear bond strength, microleakage, and film thickness of resin cement for porcelain laminate veneer. Dentin & enamel surfaces were treated with three dentin bonding agents such as Scotchbond 2. All Bone 2, and Prisma Universal Bold 2 and were cemented by G-Cera cement for porcelain laminate veneer. For bond strength test, the specimens were stored in 37℃, 100% humidity for 48 hours and shear bond strength was measured with Instron testing machine (model no. 4201) at crosshead speed of 1.0mm/min. For microleakage and film thickness measurement, the specimens were stored in 37℃, 100% humidity for 7 days, thermocycled for 1000 cycles. Microleakage and film thickness of resin cement were measured with inverted metallurgical microscope(PME 311, Olympus, Japan). The results were as follows : 1. The shear bond strength of resin cement for porcelain laminate veneer to dentin was enhanced by the use of dentin bonding agent and All Bond 2 were effective in improving the shear bond strength to both enamel and dentin. 2. All Bond 2 were effective in reducing microleakage of veneers bonded to dentin, approximately to the same degree to the veneers bonded to enamel. But Prisma Universal Bond 2 and Scotchbond 2 failed to limit microleakage at the gingival margin of veneers bonded to dentin. 3. the 40% phosphoric acid in G-Cera system and the 10% phosphoric acid in All Bond 2 appeard equally effective in reducing microleakage of veneers bonded to enamel. 4. There were no significant increase in cement film thickness when using Prisma Universal Bold 2 or Scotchbond 2, but with All Bond 2 slight increase in the cement film thickness were present.

조혈모세포이식술이 골수기질세포의 기원 및 조골세포로의 증식 및 분화에 미치는 효과

강무일,조성원,오은숙,백기현,이원영,오기원,김혜수,한제호,윤건호,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

영역분리 알고리즘을 이용한 자료의 자동 입력기법

최길성,정혜경,신재룡,곽윤식,조기형 충북대학교 컴퓨터 정보통신 연구소 1997 컴퓨터정보통신연구 Vol.5 No.1

In the GIS(Geographic Information System), the construction of a map-database requires vast expense and efforts. The manual and semi-automatic digitizing techniques which are generally used to make a digital map nowadays have limit to productivity, even to experts. Also, the existing methods have problems in maintaining consistent data precision by individual character of operators. In this thesis, we propose a new technique that automatically constructs a map-database by using a domain segmentation algorithm. The proposed technique minimizes the problems of the existing techniques. The reason is that it separates text and pictures domain automatically for coverages and the constructs data for each coverage.

홍삼류의 섭취가 비만과 혈중 지질의 상호관계에 미치는 영향

박화진,이정희,이소진,함혜선,조현정,임창률,유영빈,박기현 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.4

비만은 동맥경화의 위험인자로서 혈중에 triglyceride의 농도를 종가시키고, 상대적으로 HDL-Cholesterol의 농도를 감소시킴과 동시에 수축기 혈압을 상승시킨다. 본 연구에서 홍삼제품류를 4년 또는 5년 동안 복용해 온 건강한 사람 (ginseng군)과 홍삼제품을 복용하지 않은 건강한 사람 (control군)을 대상으로 하여 신체 계측치로부터 비만지수를 구하고 이것과 혈중의 triglyceride (TG)농도, TG/HDL-Cholesterol ratio 및 수축기 혈압과의 상호관계를 연구한 바, ginseng군에서는 대조군에 비해 혈중의 TG 농도가 일정하게 유지되었고, TG/HDL-Cholesterol ratio 및 수축기 혈압도 일정하게 유지되었다. 이러한 현상은 ginseng군에서 기호품으로 alcohol을 섭취하거나, 흡연을 한 경우에도 상관없이 일정하게 유지 되었다. 이 결과는 홍삼제품류를 장기 복용하면 비만의 저하 및 고혈압 또는 동맥경화의 위험인자가 억제될 수 있고, 결론적으로 비만, 고혈압 및 동맥경화를 예방할 수 있을 것으로 추정한다. Obesity is the risk fcactor of atherosclerosis and not only increases triglyceride concentration in blood but also decreases relatively the ratio of TG to HDL-Cholesterol in blood. In case of obesity, systolic blood pressure is also increased in responding the increase of TG in blood. Index of obesity in red ginseng-taking group (ginseng group) was lower as compared with non-red ginseng-taking group (control group). The TG concentration, the ratio of triglyceride to HDL-cholesterol in blood and systolic blood pressure were decreased in the subjects of ginseng group compared with that in control group. It is inferred that long-term intake of ginseng products may help to prevent the risk of atherosclerosis and obesity.