벼와 피에서 제초제 alachlor의 선택적 작용

황을철,우승희 東亞大學校生命資源科學大學附設 農業資源硏究所 1995 農業生命資援硏究 Vol.4 No.1

벼와 피에서 α-chloroacetamide계 제초제의 선택적 작용, 특히 포장에 이앙하는 어린모의 벼(약 40일묘)와 발아중인 피에서 alachlor의 선택적 작용이 발현되는 근거를 찾고자, 벼와 피에서 몇가지 생화학적 차이를 조사하였다. 제초제 alachlor에 의한 약해는 생장일수가 동일한 유묘기의 벼와 피에서는 비슷하였으나, 생장일수가 서로 다른 40일묘의 벼와 7일묘의 피에서는 현격한 차이가 있었다. Alachlor에 대하여 약해정도가 현격히 다른 40일묘의 벼와 7일묘의 피체내의 GSH 함량과 GST 활성은 서로 비슷하였으며, alachlor 처리에 의한 GSH 함량과 GST 활성의 증가도 모두 미미하였다. 40일묘의 벼와 7일묘의 피에서 alachlor 처리에 의해 저해되는 α-amylase의 활성은 무처리에 비해 각각 93.5%와 70%로 현격한 차이를 나타내었으며, 이는 약해정도와 부합하였다. 따라서, 40일묘의 벼와 7일묘의 피에서 발현되는 alachlor의 선택성 또는 벼를 이앙한 포장에서 발아중인 피를 방제하는 α-chloroacetamide계 제초제의 선택성은, 벼와 피체내의 방어계인 glutathione conjugation 반응에 기인하는 것이라기 보다는, 종실내의 주된 저장영양분인 탄수화물의 이용에 관련된 효소인 α-amylase가 저해되는 정도에 기인하는 것으로 판단되었다. Several biochemical differences between rice and barnyardgrass which were related to glutathione conjugation and utilization of starch reserves in seeds were examined to investigate the selectivity of α-chloroacetamide herbicides in rice and barnyardgrass, especially in transplanting staged rice (about 40-day-old) and germinating barnyardgrass. Phytotoxicities of alachlor to rice seedlings and barnyardgrass seedlings were all severe at stronger concentration that 1.0×10-6 and were similiar to each other at various concentrations. However, 40-day-old rice which was independent on the seed reserves was tolerant to alachlor. Glutathione(GSH) content and activity of glutathione S-transferases(GST) in roots of rice seedlings and barnyardgrass seedlings were similiar to each other, and those of 40-day-old rice and barnyardgrass seedlings which were observed to show different responses to alachlor were so. Moreover, the inductions of GSH and GST in 40-day-old rice and 7-day-old barnyardgrass following treatments with 1.0×10-5 solution of alachlor were insignificant. On the other hand, activities of α-amylase in seeds of 40-day-old rice and 7-day-old barnyardgrass following treatments with 1.0×10-5 solution of alachlor decreased to 93.5% and 70.0% of the control respectively. These results were consistent in phytotoxicities above stated. It was therefore stated that the selectivity of alachlor, α-chloroacetamide herbicide in 40-day-old rice and germinating barnyardgrass was due to inhibition degree of α-amylase needed for starch utilization as energy sources in grass germlings, rather than due to the rate of glutathione conjugation generally considered as main factor contibuting to α-chloroacetamide herbicide detoxification and selectivity.

사과, 복숭아와 감중의 몇가지 농약의 잔류평가

황을철,김정기 동아대학교 환경문제연구소 1993 硏究報告 Vol.16 No.2

Apples, peaches, and persimmons collected from their chief producing districts and trading centers in 1992 respectively were analyzed for chlorothalonil, fenitrothion, and phenthoate residues by glc equipped with a electrn capture detector or a nitrogen phosphorus detector. The results obtained are as follows : Average and range of chlorothalonil residue levels n apples was 0.008ppm and ND-0.030ppm, and in peaches 0.010ppm and 0.073ppm, respectively, while no chlorothalonil was detected in persimmons. No organophosphorus pesticides such as fenitothion, chlorpyrifos, and phenthoate were detected in all fruits. In all fruits, even the maximum residue level, not to mention the average, of four pesticides examined was much lower than its maximum residue limit(MRL) respectively.

水稻幼苗와 土壤에서 ??C標識 Diazinon의 去就

李成桂,金均,黃乙喆,朴昌奎 서울大學校 農科大學 1985 서울대농학연구지 Vol.10 No.2

The fate of diazinon in the intact rice plants and submerged paddy soil has been investigated with (??) diazinon, The labelled diazinon solution was applied to paddy water and distribution of radioactivities in the rice seedlings, paddy soil, volatile fraction and carbon dioxide has been ascertained at end the of incubation times of 0.5,1,4,6 and 9 days respectively. In addition, extract of plants and paddy soils were subjected to TLC separation for examination of possible transformation products of diazinon. The results may be summarized as follows; 1. Total recoveries of radiactivities were between 57.2~73.6 per cent. 2. Radioactivity in rice seedlings increased with incubation periods reaching one tenth of treated radioactivity at the end of 9 day incubation. 3. Non-extractable radioactivity in paddy soil increased with incubation periods. 4. Radioactive volatile fraction increased in the presence of rice seedlings. 5. Pyrimidinol was unique conversion product of diazinon in rice seedlings and paddy soils. 6. Pyrimidinol applied to paddy soil is readily absorbed by rice seedlings.

괴사 근막염이 합병된 류마티스 관절염

황준일 ( Jun Eul Hwang ),우학 ( Hak Woo ),김상호 ( Sang Ho Kim ),이우석 ( Woo Seok Lee ),황재하 ( Jae Ha Hwang ),김정철 ( Jung Chul Kim ),조덕 ( Duck Cho ),박정진 ( Jeong Jin Park ),이신석 ( Shin Seok Lee ),박용욱 ( Yong Wook Pa 대한류마티스학회 2006 대한류마티스학회지 Vol.13 No.2

Necrotizing fasciitis is a life-threatening soft tissue infection involving skin, subcutaneous tissue, and superficial fascia. We report a case of necrotizing fasciitis that developed in a 76-year-old female patient taking low-dose methotrexate and prednisolone for rheumatoid arthritis (RA). A computed tomography scan of the neck during the initial work-up showed soft tissue swelling, loss of fat planes, and mild heterogeneous enhancement in the right lateral neck, suggesting cellulitis. The lesions were associated with skin necrosis and multiple bullae rapidly expanded to the right anterior chest in spite of empirical antibiotic therapy. Surgical debridement was immediately performed, and soft tissue biopsy from the lesions showed the pathologic finding consistent with necrotizing fasciitis. The skin wound defect was reconstructed by a flap operation with split-thickness skin graft. This case shows the development of necrotizing fasciitis in a elderly patient taking methotrexate and low-dose steroid for RA and highlights early recognition and prompt surgical debridement for successful management.

Paraquat 저항성 망초의 protective 효소

황을철,김희주 한국농화학회 2000 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.1

The resistance of Cornyza bonariensis to herbicide paraquat was investigated by evaluating the activities of three enzymes concerning in scavenging paraquat-generated toxic oxygen species such as superoxide radical and hydrogen peroxide in resistant and susceptible biotypes. Conyza bonariensis inhabited in cultivated area was more tolerant to paraquat than that of uncultivated area. This is the first report that a biotype of Cornyza bonariensis has appeared in an area with repeated paraquat treatments of Korea. Superoxide dismutase activity of resistant biotype was 20% higher as 150 than that of susceptible biotype. Ascorbate peroxidase activity of resistant biotype was 44% higher than that of susceptible biotype. Glutathione reductase activity of resistant biotype was 64% higher than that of susceptible biotype. It can be concluded from above results that the resistance of Conyza bonariensis to paraquat depends partially on the toxic oxygen species-scavenging efficiency of protective multienzymatic system which is composed of three enzymes, superoxide dismutase, ascorbate peroxidase, and glutathione reductase.

Overexpression of Zinc Finger Protein of Capsicum annuum(PIF1) in Tobacco Enhances Cold Tolerance

Eul Won Hwang,Soo Chul Park,Myung Ok Byun,Mie Young Choi,Hawk Bin Kwon 한국유전학회 2008 Genes & Genomics Vol.30 No.2

Plants encounter various stresses from their surrounding environment, such as low temperature, drought and high salinity. Thus the plant responds to abiotic stress such as cold and drought through a range of different strategies including physiological and biochemical processes that are usually mediated by up-regulation of the expression of stress regulated genes. Zinc finger proteins play a role in abiotic stress tolerance such as cold, dehydration and salt. As one way to approach to cold defense mechanism in plants, we previously identified a gene for zinc finger protein (PIF1) from hot pepper (Capsicum annuum) as cold inducible using cDNA microarray analysis coupled with Northern blot analysis. We previously showed that the PIF1 gene was induced strongly by cold stress, slightly by ABA. The PIF1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting PIF1 transgenic tobacco plants showed significantly increased cold stress resistance. In addition, all PIF1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results suggest the biological role of PIF1 in plant stress tolerances.

Expressing the Tyrosine Phosphatase (CaTPP1) Gene from Capsicum annuum in Tobacco Enhances Cold and Drought Tolerances

Eul Won Hwang,Soo Chul Park,Mi Jeong Jeong,Myung Ok Byun,Hawk Bin Kwon 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.2

혀에 발생한 화농성 육아종 1예

황을상,박재홍,계영철,김수남 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.11

Pyogenic granuloma is a relatively common entity fmt described by Poncet and Dor in 1897 as 'human botryomycosis'. It may occur anywhere on the skin, including the mucosal surface, but is most common on the fingers, around the nails, on the face and on the scalp. The tongue is a rare location for its occurrence. There is controversy as to etiology of pyogenic granuloma and conservative excision is the treatment of choice. We describe a 41-year old woman with a solitary protruding nodule on the tongue that was treated by excision and electric cautery. (Korean J Dermatol 2003;41(11) : 1557-1559)

Er:YAG 레이저 박피술 후 발생한 저색소침착증

황을상 ( Eul Sang Hwang ),우상혁 ( Sang Hyuk Woo ),성환태 ( Hwan Tae Sung ),김수남 ( Soo Nam Kim ),계영철 ( Young Chul Kye ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.7

간 및 위 , 장관을 침범한 특발성 과호산구 증후군 치험

정을순(Eul Soon Chung),이상종(Sang Jong Lee),김향(Hyang Kim),김병익(Byung Ik Kim),황기은(Kie Eun Hwang),성기철(Ki Chul Sung),조용균(Yong Kyun Cho),이희무(Hee Moo Lee),금주섭(Joo Seob Keum) 대한소화기학회 1997 대한소화기학회지 Vol.30 No.3

Marked eosinophilia is found in many kinds of disease state, It usually occur in allergic and parasitic conditions and occ assionally in autoimmune disease, blood dyscrasias, or certain malignancics. Idio- pathic hypereosinophilic syndrome is characterized by markedly elevated peripheral blood eosinophil count without explainable etiology. Eosinophilopoiesis is related with T-lymphocyte activation and cytokine cascade controlling eosinophilic production. It shows tissue infiltration in many organs including endomyocardium, lung, liver, gastrointestinal tract. We have recently experienced a case with idiopathic hypereosinophilic syndrome of a 65-year-old woman who had of complained anorexia, weight loss and diarrhea during the past month and half. Persistantly increased eosinophil count was shown in her peripheral blood, the count averaging 3480/mm'(41% in differential count). Abdominal computer tomography disclosed multiple small relatively ill-defined hypoechoic lesions in the liver. Microscopically, the needle biopsy of the liver revealed heavy infiltration by eosinophilis especially in the portal tracts. On reviewing, previously perfomed endoscopic biopsies from the stomach and terminal ileum also had mild to moderate eosinophilic infiltration, respectively. No evidence of parasitic infection was present on the clinical history and laboratory test. Corticosteroid was administered ard brought marked improvement of clinical manifestations including both hematologic and radiologic abnormalities. (Korean J Gastroenterol 1997; 30:397- 403)