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Hawk-Bin Kwon,Eul-Won Hwang,정종주 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.5
EREBP, ethylene responsive element binding protein, plays a role in plant tolerance to abiotic stresses such as low temperatures, drought and high salinity. We previously used cDNA microarrays and northern blot analysis to determine the mechanisms responsible for those underlying defenses. These analyses led to identification of CaEREBP-C4, a gene that encodes the ethylene responsive element binding protein from hot pepper (Capsicum annuum). In that study, we demonstrated that the CaEREBP-C4 gene was strongly induced by cold stress. Here, we used Ti-plasmid and Agrobacterium-mediated transformation to engineer CaEREBP-C4 under control of the CaMV 35S promoter for constitutive expression in transgenic tobacco. The resultant CaEREBP-C4 transgenic plants exhibited significantly increased tolerance to low temperature. Moreover, the transgenic plants that showed strong cold tolerance also had greater tolerance to drought stress. In addition, none of the CaEREBP-C4 transgenic plants showed visible phenotypic alteration when compared to wild type plants. Taken together, these results suggest that CaEREBP-C4 plays a biological role in conferring plant abiotic stress tolerance.
Expression of the RSI-1 Gene during Development of Roots and Reproductive Organs in Tomato
Kwon, Hawk Bin,Lee, Gyung Hee,Cheong, Jong Joo 한국식물학회 1999 Journal of Plant Biology Vol.42 No.4
The RSI-1 gene is expressed in pericycle cells just prior to the first round of cell division for lateral root development in tomato. We transformed tomato plants with the RSI-1 gene promoter linked to a GUS reporter gene. GUS activity was detected not only at the sites of initiation for lateral and adventitious roots, but also at the primary root tip. Expression of the fusion gene was also regulated at various stages of tissue development: in particular, during the formation of reproductive organs such as pollen and fruit Overexpression of the RSI-1 gene in either the sense or anti-sense orientation resulted in arrest of fruit development and seed germination. The RSI-1 gene product, therefore, may play a role in auxin-induced cell division in various developing tissues. Inter- and intramolecular disulfide bridges between cysteines rich in the RSI-1 protein might be involved in cell-wall modifications that are essential for new cell division. These hypotheses for the role of the RSI-1 gene in lateral-root and reproductive-organ development remain to be tested.
Kwon, Hawk-Bin,Hwang, Eul-Won,Cheong, Jong-Joo The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.5
EREBP, ethylene responsive element binding protein, plays a role in plant tolerance to abiotic stresses such as low temperatures, drought and high salinity. We previously used cDNA microarrays and northern blot analysis to determine the mechanisms responsible for those underlying defenses. These analyses led to identification of CaEREBP-C4, a gene that encodes the ethylene responsive element binding protein from hot pepper (Capsicum annuum). In that study, we demonstrated that the CaEREBP-C4 gene was strongly induced by cold stress. Here, we used Ti-plasmid and Agrobacterium-mediated transformation to engineer CaEREBP-C4 under control of the CaMV 35S promoter for constitutive expression in transgenic tobacco. The resultant CaEREBP-C4 transgenic plants exhibited significantly increased tolerance to low temperature. Moreover, the transgenic plants that showed strong cold tolerance also had greater tolerance to drought stress. In addition, none of the CaEREBP-C4 transgenic plants showed visible phenotypic alteration when compared to wild type plants. Taken together, these results suggest that CaEREBP-C4 plays a biological role in conferring plant abiotic stress tolerance.
Seon-Ju Shin,Jae-Hee Lee,Hawk-Bin Kwon 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Abiotic environmental stresses cause serious economic losses in agriculture. These stresses include temperature extremes, high salinity and drought. We identified several drought stress-related novel/function unknown coding transcripts (transcription factors and functional genes) and non-coding transcripts (small noncoding transcripts such as microRNA and long noncoding transcripts) using the next generation sequencing method from rice (Oryza sativa L.), and have constructed databases of drought stress-related coding and noncoding transcripts. We used novel gene prediction programs for the selections. The expression level of the each gene was analyzed by real-time PCR. The results ended up the selection of 29 transcription factors, 6 microRNAs and 10 long noncoding RNAs. Currently, we are further characterizing these transcripts. We expect that this study could provide functional information of the drought stress-regulated novel genes, and relationships among novel coding and noncoding transcripts.
Kwon, Soon-Jae,Hwang, Eul-Won,Kwon, Hawk-Bin 한국유전학회 2004 Genes & Genomics Vol.26 No.2
Trehalose has been known in many organisms as an energy source and a protectant against several environmental stresses such as freezing, osmotic pressure, heat and desiccation. Trehalose synthesis is mediated by trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) enzyme complex in yeast. In this study, TPS1 and TPS2 genes of Zygosaccharomyces rouxii was engineered under the control of the CaMV 35S promoter along with 2A self-cleavage sequence for constitutive expression in transgenic potato plants by Ti-plasmid of Agrobacterium transformation. The resulting construct, ZrTPS2-2A-ZrTPS1, appeared to be transcribed as a single transcript. On the contrary to the previous result that TPS1 transgenic plant showed some morphological alteration, transgenic ZrTPS2-2A-ZrTPS1 potato plant showed no phenotype alteration both in test tube and soil mixture. Most importantly, the transgenic ZrTPS2-2A-ZrTPS1 potato plant showed very strong drought tolerancy. We showed the multigene expression system using FMDV 2A sequence could be very useful for two or more gene expression under the control of one promoter and at the same expression level.
Arabidopsis thaliana ga3 돌연변이체에서 지베렐린에 의해 발현이 유도되는 유전자의 분리 및 특성 연구
권혁빈 한국유전학회 1999 Genes & Genomics Vol.21 No.3
We have isolated a novel gibberellin (GA)-regulated gene, designated gene 107, using mRNA differential display reverse transcription PCR in GA-deficient ga3 mutants of Arabidopsis in an effort to identify GA-regulated genes. Gene 107 was induced 4-fold 8 hr after spraying with 10 μM GA₃ solution followed by a 2-fold increase 24 hrs after GA treatment, then maintained 4-fold elevated expression for up to 96 hrs after GA treatment in 10-day old ga3 plants. However, in 10-day old wild type plants, gene 107 was not induced by GA treatment. Gene 107 was also induced by ABA in 10-day old ga3 mutant plants. Gene 107 was strongly expressed in rosette leaves, roots and flowers, but not in green siliques in wild type plants. Gene 107 expression was induced by GA in 10 day-old ga2 and ga3 mutant Arabidopsis by 4- and 5-fold, respectively, and a slight induction (1.5 to 2 fold) was observed in ga5 and gai mutants. However induction of gene 107 was not detected in ga1 and ga4 mutants. A full length cDNA clone of gene 107 consists of 1,257 nucleotides. Sequence analysis detected weak homology to a putative Arabidopsis β-amylase gene. The cDNA of gene 107 is predicted to encode a 36.6 kD protein consisting of 335 amino acid residues. To our knowledge, gene 107 is the first example of a gene that is inducible by both GA and ABA in the ga mutant background.
Cheong, Jong-Joo,Kwon, Hawk-Bin The Korean Society for Applied Biological Chemistr 2005 Journal of Applied Biological Chemistry (J. Appl. Vol.48 No.4
Arabidopsis AHL gene encodes a 3'(2')-phosphoadenosine 5'-phosphate (PAP)-specific phosphatase that plays a role in the sulfate activation pathway. We complemented E. coli cysQ mutant defective in cysteine biosynthesis with the AHL gene. AHL cDNA was cloned into the prokaryotic expression vector pKK388-1 and transformed into the bacterial mutant. Since cysQ mutant is a leaky cysteine auxotroph only under aerobic conditions, the bacteria were grown in liquid media with vigorous shaking to provide more aeration. In cysteine-free medium, cysQ mutant and the mutant harboring empty vector did not grow well, whereas cells harboring AHL cDNA exhibited significantly improved growth with doubling time of approximately 3 h. cysQ is known to encode a 3'(2'),5'-diphosphonucleoside 3'(2')-phosphohydrolase (DPNPase). However, our data suggest that cysQ protein has PAP-specific phosphatase activity in addition to DPNPase activity. Microbial complementation procedure described in this paper is useful for structure-activity studies of PAP-specific phosphatases identified from microbes and plants.
Structural Characteristics of the Putative Protein Encoded by Arabidopsis AtMTN3 Gene
Cheong, Jong-Joo,Kwon, Hawk-Bin,Kim, Minkyun 한국응용생명화학회 2001 Journal of Applied Biological Chemistry (J. Appl. Vol.44 No.3
A putative protein encoded by Arabidopsis AtMTN3 gene, a homologue of Medicago truncatula MTN3, consists of 285 amino acid residues, and has a predicted molecular mass of 31.5 kDa and a calculated pI of 9.1. Primary amino acid sequence analyses have revealed that the protein contains seven putative transmembrane regions with N-terminus oriented to the outside of the membrane. The AtMTN3 protein shows overall 16.4% of amino acid identity with the rat GALR3 protein, known to be a G-protein-coupled receptor. The gene is present as a single copy in the Arabidopsis genome, and expressed in aerial parts but not in roots of Arabidopsis. Therefore, AtMTN3 appears not to be specifically involved in Rhizobium-induced nodule development, as was predicted for the MTN3 gene. These proteins possibly mediate signal transmission through G-protein-coupled pathways during general interactions between plants and symbiotic or pathogenic microbes.