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Jingtian Wang,Lei Fang,Qidi Wu,Dongdong Li,Zhongming Huo,Xiwu Yan 한국유전학회 2020 Genes & Genomics Vol.42 No.1
Introduction AMP-activated protein kinases (AMPK) are heterotrimeric complexes. The main upstream phosphorylase has AMP-dependent LKB1 and Ca2+-dependent CaMKK beta. AMPK also includes an auto-inhibitory domain and a region associated with beta and gamma subunits, which regulate a variety of cellular activities and energy metabolism. The increase in the ratio of AMP/ATP can stimulate the activation of AMPK. Once AMPK is activated, pathways to ATP consumption (e.g., fat, cholesterol, and protein synthesis) will be shut down. The pathway to ATP generation (e.g., oxidation of fat and glycolysis pathway) will be activated. AMPK genes have not been systematically characterized in marine invertebrates. Methods In this study, we identified and characterized three AMPK genes, AMPK-α, AMPK-β, and AMPK-γ, in the Manila clam (Ruditapes philippinarum). To gain insight into the role of AMPK genes during clam energy metabolism, quantitative real-time PCR was used to investigate the expression profiles in the different stages of clam development, in healthy adult tissues, and after air exposure at two different temperatures. Results Phylogenetic and protein structural analyses were conducted to determine the identity and evolutionary relationships of these genes. The structural features of the genes were relatively well-conserved, relative to the AMPK genes of other vertebrates. The expression of genes was significantly induced 3–48 h after air exposure. Conclusinon AMPK-α, AMPK-β and AMPK-γ are involved in clam energy metabolism. Increased expression levels of AMPK genes in the gill and intestine of Manila clam in response to air exposure implied a strong adaptability to the coastal environment.
Huiqin Wang,Dongyao Wu,Chongyang Liu,Jingru Guan,Jinze Li,Pengwei Huo,XINLIN LIU,Qian Wang,Yongsheng Yan 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.67 No.-
The purpose of this work designed hybrid-structured and plasma effect photocatalyst of Ag/In2O3/TiO2/HNTs via sol–gel and photo-reduction methods. The structures, morphologies, optical and photoelectric performances of as-prepared photocatalysts were characterized via XRD, TEM, XPS, BET, UV–vis DRS, PL and photocurrents. The photocatalytic activity was evaluated by degradation of TC. The results showed that the hybrid-structure and plasma effect can effectively cause the multi-transfer of electrons and increase the separation rate of electron and hole pairs which obtained high photocatalytic activity. The photocatalytic degradation processes reveal that O2− and h+ are major active species.
Pengwei Huo,Chongyang Liu,Dongyao Wu,Jingru Guan,Jinze Li,Huiqin Wang,Qi Tang,Xiuying Li,Yongsheng Yan,Shouqi Yuan 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
In this work, we reported one kind of photocatalytic and antibacterial materials, Ag/Ag2S/reducedgraphene oxide (rGO), which prepared by the hydrothermal and UV-light-assisted reduction methods. The Ag2S nanoparticals were well dispersed on the surface of rGO as revealed by series ofcharacterization. The obtained Ag/Ag2S/rGO photocatalyst showed excellent photocatalytic degradationefficiency of 87.6% for ciprofloxacin (CIP) under visible light irradiation and outstanding antibacterialactivity for Escherichia coli (E. coli) which reached to 97.76% in 24 h. The trapping of active species showedthat the hydroxyl radicals, holes and superoxide radicals played important roles in the photocatalyticdegradation of CIP processes.
Huiqin Wang,Xiaoqing Yang,Jinzhao Zi,Mingjun Zhou,Zhefei Ye,Jinze Li,Qingfeng Guan,Peng Lv,Pengwei Huo,Yongsheng Yan 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.35 No.-
Ag/AgCl/activated carbon (AC) plasmonic photocatalysts were prepared by precipitation and photoreduc-tion method. The as-prepared plasmonic photocatalysts were characterized by XRD, SEM, BET, EDS, UV–visDRS, XPS and PL. The results show the composite photocatalysts are successfully obtained and obviouslyexhibit surface plasmon resonance and effectively enhance the separation of h+/e . The photocatalyticdegradation rate of tetracycline (TC) could reach 97.3% in 60 min under visible light irradiation and cycledexperiments show stably photocatalytic activities. The active species analysis displays holes andsuperoxide radicals are the main reactive species. Photocatalytic kinetic indicates that the degradationprocesses obey pseudo first order kinetic equation.
Huiqin Wang,Pengwei Huo,Jinze Li,Mingjun Zhou,Qingfeng Guan,Ziyang Lu,Yongsheng Yan 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.30 No.-
The Ag2O/SWNTs photocatalysts were prepared by precipitated method, the photocatalysts werecharacterized by XRD, SEM, TEM, EDS, BET, UV–vis DRS, XPS, PL and Raman. The photocatalytic activitieswere evaluated by degradation of tetracycline. The results showed that the composited photocatalystswere successfully prepared, the responded light was enhanced with increasing the supported dosage ofAg2O, and the photocatalytic activity was also improved. The enhanced photocatalytic activity wasattributed to the synergy effect of SWNTs and Ag2O, the recycled degradation of tetracycline exhibitedstability of Ag2O/SWNTs composited photocatalysts. Finally, the possible mechanism for transferredprocesses of photoelectrons and photoholes were proposed.
Heavy concrete shielding properties for carbon therapy
Jin-Long Wang,Jiade J Lu,Da-Jun Ding,Wen-Hua Jiang,Ya-Dong Li,Rui Qiu,Hui Zhang,Xiao-Zhong Wang,Huo-Sheng Ruan,Yan-Bing Teng,Xiao-Guang Wu,Yun Zheng,Zi-Hao Zhao,Kai-Zhong Liao,Huan-Cheng Mai,Xiao-Dong Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6
As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm<sup>3</sup> to 4.14 g/cm<sup>3</sup>, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm<sup>3</sup>, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm<sup>3</sup> with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.
Research on Rapid Detection Method of Protein and Fat in Raw Milk Based on Mid-infrared Spectrum
Xiaoming Li,Guicheng Huo,Yan Wang,Hongmin Sun,Qingming Kong 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10
In this paper raw milk was taken as the research object, using mid-infrared spectrum analysis method rapidly tests protein and fat content in raw milk through establishing quantitative analysis model. First of all, researchers collect 84 kinds of raw milk which is different areas and varieties in Heilongjiang province, and classify 84 samples into calibration sets and validation sets by standard chemical testing and spectral scanning on protein and fat, and calibration sets contain 64 samples, and validation sets contain 20 samples. Then, denoising by second derivative method which window size is 17 points, and selecting method by comparing SPA and GA characteristic wavelength respectively, and establishing PLS model and model demonstration. Eventually, researchers draw the conclusion that SPA wavelength selection method which value of m_max is 10 and GA wavelength selection method which value of Evaluat is 10d can effectively improve validation set model precision, but precision of the former is higher and characteristic wavelength is least, and its characteristic wavelength point reduces from 220 to 4, R2 of protein model validation set is 0.8010929, RMSEP is 0.0207505, R2 of fat model validation set is 0.91007989, RMSEP is 0.045329, relative standard deviation RSD<3%. These prove that the method for rapid testing of raw milk components is feasible.
Research on Rapid Detection Method of Dry Matter Content in Raw Milk Based on Mid-infrared Spectrum
Xiaoming Li,Guicheng Huo,Yan Wang,Qingming Kong,Hongmin Sun 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.9
This paper takes raw milk as research object, and using mid-infrared spectrum analysis method rapidly tests dry matter in raw milk through establishing quantitative analysis model. First of all, researchers collect 84 kinds of raw milk which is different areas and varieties in Heilongjiang province, and classify 84 samples into calibration sets and validation sets by standard chemical testing and spectral scanning on raw milk, and calibration sets contain 64 samples, and validation sets contain 20 samples. Then, respectively selecting SPA and GA characteristic wavelength after treating by smoothing denoising method which window size is 11 points, and establishing PLS model and model demonstration. Eventually, researchers draw the conclusion that SPA wavelength selection method which value of m_max is 10 and GA wavelength selection method which value of Evaluat is 10d can effectively improve validation set model precision, but precision of the latter is higher and characteristic wavelength point reduces from 352 to 10, The calibrating determination coefficient R2 of model is 0.8092155, root mean square error (RMSEC ) is 0.1206172. Validation set determination coefficient R2 is 0.8620867, the root mean square error (RMSEC) is 0.0950656, and relative standard deviation RSD<3%. These prove that the method for rapid testing of raw milk components is feasible.
Huang, Xin-En,Wei, Guo-Li,Huo, Jie-Ge,Wang, Xiao-Ning,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Xiang, Jin,Feng, Ji-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4
Aims: To explore efficacy and side effects of intrapleural or intraperitoneal lobaplatin for treating patients with malignant pleural or peritoneal effusions. Methods: Patients in Jiangsu Cancer Hospital and Research Institute with cytologically confirmed solid tumors complicated with malignant pleural effusion or ascites were enrolled into this study. Lobaplatin (20-30 $mg/m^2$) was intrapleurally or intraperitoneally infused for patients with malignant pleural effusion or ascites. Results: From 2012 to 2013, intrapleural or intraperitonea lobaplatin was administered for patients with colorectal or uterus cancer who were previous treated for malignant pleural effusion or ascites. Partial response was achieved for them. Main side effects were nausea/vomiting, and bone marrow suppression. No treatment related deaths occurred. Conclusion: Intrapleural or intraperitoneal infusion of lobaplatin is a safe treatment for patients with malignant pleural effusion or ascites, and the treatment efficacy is encouraging.