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Jingtian Wang,Lei Fang,Qidi Wu,Dongdong Li,Zhongming Huo,Xiwu Yan 한국유전학회 2020 Genes & Genomics Vol.42 No.1
Introduction AMP-activated protein kinases (AMPK) are heterotrimeric complexes. The main upstream phosphorylase has AMP-dependent LKB1 and Ca2+-dependent CaMKK beta. AMPK also includes an auto-inhibitory domain and a region associated with beta and gamma subunits, which regulate a variety of cellular activities and energy metabolism. The increase in the ratio of AMP/ATP can stimulate the activation of AMPK. Once AMPK is activated, pathways to ATP consumption (e.g., fat, cholesterol, and protein synthesis) will be shut down. The pathway to ATP generation (e.g., oxidation of fat and glycolysis pathway) will be activated. AMPK genes have not been systematically characterized in marine invertebrates. Methods In this study, we identified and characterized three AMPK genes, AMPK-α, AMPK-β, and AMPK-γ, in the Manila clam (Ruditapes philippinarum). To gain insight into the role of AMPK genes during clam energy metabolism, quantitative real-time PCR was used to investigate the expression profiles in the different stages of clam development, in healthy adult tissues, and after air exposure at two different temperatures. Results Phylogenetic and protein structural analyses were conducted to determine the identity and evolutionary relationships of these genes. The structural features of the genes were relatively well-conserved, relative to the AMPK genes of other vertebrates. The expression of genes was significantly induced 3–48 h after air exposure. Conclusinon AMPK-α, AMPK-β and AMPK-γ are involved in clam energy metabolism. Increased expression levels of AMPK genes in the gill and intestine of Manila clam in response to air exposure implied a strong adaptability to the coastal environment.
Fei Yuchen,Ye Xiaofang,Al-Baldawy Aseel Shaker,Wan Jing,Lan Jinshen,Zhao Jingtian,Wang Ziyun,Qu Shanzhi,Hong Rongdun,Guo Shengshi,Huang Shengli,Li Shuping,Kang Junyong 한국물리학회 2022 Current Applied Physics Vol.44 No.-
Noble metal particles have been embedded in semiconductors to improve photocatalysis efficiently, but the high cost made this approach difficult to apply widely in industry. Herein titanium dioxide/reduced graphene oxide (TiO2/rGO) nanowires in a core-shell structure were prepared. The physicochemical properties and photocatalytic performance of the specimen were characterized in comparison with TiO2 and TiO2/Pt nanowires. The rGO layer and Pt nanoparticles increased chemical states of the components, reduced bandgap energy of the nanowires, enhanced visible light absorption, improved conductance and capacitance significantly. The methylene blue as catalyzed by TiO2/Pt and TiO2/rGO nanowires was degraded to 7.9% and 8.4% in an hour, but retained 25.7% by the TiO2 nanowires. The properties and function of TiO2/rGO nanowires were close to those of TiO2/Pt nanowires, while the rGO price was much lower than that of Pt, which was of great significance for the photocatalytic application of TiO2 heterojunction materials in industry.