Ginsenoside Rb1 exerts neuroprotective effects through regulation of Lactobacillus helveticus abundance and GABA<SUB>A</SUB> receptor expression

Huimin Chen,Jiajia Shen,Haofeng Li,Xiao Zheng,Dian Kang,Yangfan Xu,Chong Chen,Huimin Guo,Lin Xie,Guangji Wang,Yan Liang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1

Background: Ginsenoside Rb1 (Rb1), one of the most abundant protopanaxadiol-type ginsenosides, exerts excellent neuroprotective effects even though it has low intracephalic exposure. Purpose: The present study aimed to elucidate the apparent contradiction between the pharmacokinetics and pharmacodynamics of Rb1 by studying the mechanisms underlying neuroprotective effects of Rb1 based on regulation of microflora. Methods: A pseudo germ-free (PGF) rat model was established, and neuroprotective effects of Rb1 were compared between conventional and PGF rats. The relative abundances of common probiotics were quantified to reveal the authentic probiotics that dominate in the neuroprotection of Rb1. The expressions of the gamma-aminobutyric acid (GABA) receptors, including GABAA receptors (α2, β2, and γ2) and GABAB receptors (1b and 2), in the normal, ischemia/reperfusion (I/R), and I/RþRb1 rat hippocampus and striatum were assessed to reveal the neuroprotective mechanism of Rb1. Results: The results showed that microbiota plays a key role in neuroprotection of Rb1. The relative abundance of Lactobacillus helveticus (Lac.H) increased 15.26 fold after pretreatment with Rb1. I/R surgery induced effects on infarct size, neurological deficit score, and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) were prevented by colonizing the rat gastrointestinal tract with Lac.H (1×10<SUP>9</SUP> CFU) by gavage 15 d before I/R surgery. Both Rb1 and Lac.H upregulated expression of GABA receptors in I/R rats. Coadministration of a GABAA receptor antagonist significantly attenuated neuroprotective effects of Rb1 and Lac.H. Conclusion: In sum, Rb1 exerts neuroprotective effects by regulating Lac.H and GABA receptors rather than through direct distribution to the target sites.

Dietary phosphorus deficiency impaired growth, intestinal digestion and absorption function of meat ducks

Huimin Xu,Shujun Dai,Keying Zhang,Xuemei Ding,Shiping Bai,Jianping Wang,Huanwei Peng,Qiufeng Zeng 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: An experiment was conducted to investigate the effects of dietary non-phytate phosphorus (nPP) deficiency on intestinal pH value, digestive enzyme activity, morphology, nutrient utilization, and gene expression of NaPi-IIb in meat ducks from 1 to 21 d of age. Methods: A total of 525 one-d-old Cherry Valley ducklings were fed diets (with 7 pens of 15 ducklings, or 105 total ducklings, on each diet) with five levels of nPP (0.22%, 0.34%, 0.40%, 0.46%, or 0.58%) for 21 d in a completely randomized design. Five experimental diets contained a constant calcium (Ca) content of approximately 0.9%. Body weight (BW), body weight gain (BWG), feed intake (FI), and feed to gain ratio (F:G) were measured at 14 and 21 d of age. Ducks were sampled for duodenum and jejunum digestion and absorption function on 14 and 21 d. Nutrient utilization was assessed using 25- to 27-d-old ducks. Results: The results showed ducks fed 0.22% nPP had lower (p<0.05) growth performance and nutrient utilization and higher (p<0.05) serum Ca content and alkaline phosphatase (ALP) activity. When dietary nPP levels were increased, BW (d 14 and 21), BWG and FI (all intervals), and the serum phosphorus (P) content linearly and quadratically increased (p<0.05); and the jejunal pH value (d 14), duodenal muscle layer thickness (d 14), excreta dry matter, crude protein, energy, Ca and total P utilization linearly increased (p<0.05); however, the serum ALP activity, jejunal Na+-K+-ATPase activity, and duodenal NaPi-IIb mRNA level (d 21) linearly decreased (p<0.05). Conclusion: The results indicated that ducks aged from 1 to 21 d fed diets with 0.22% nPP had poor growth performance related to poor intestinal digestion and absorption ability; but when fed diets with 0.40%, 0.46%, and 0.58% nPP, ducks presented a better growth performance, intestinal digestion and absorption function.

UGC as a New Digital Promotion in the Metaverse Context

Huimin XU 한국유통과학회 2023 The Journal of Industrial Distribution & Business( Vol.14 No.10

Purpose: This study aims to investigate how brand-related user-generated content (UGC) influences consumers' brand attitude and behavioral intention in the context of the Metaverse, and the mediating role of virtual brand experience, perceived information usefulness, perceived interactivity, and attitude toward advertising. Research design, data and methodology: The study was conducted using a survey with 239 questionnaires from frequent social media users in China and hypothesis testing through AMOS 26.0 structural equation modeling. Results: The findings suggest that (1) brand-related UGC positively influences brand attitude and behavioral intention through virtual brand experience, perceived information usefulness, perceived interactivity, and attitude toward advertising. (2) The study identified the fully-mediated effects of virtual brand experience, perceived information usefulness, perceived interactivity, and attitude toward advertising in the impact of brand-related UGC on brand attitude and behavioral intention. (3) The mediating pathway with the most significant impact on behavioral intention was the virtual brand experience and attitude toward the advertising, followed by the effect of the virtual brand experience, perceived information usefulness, and perceived interactivity on brand attitude. Conclusions: This study presents UGC as a new type of digital promotion that can positively impact the effectiveness of brand advertising in the Metaverse.

Ginsenoside Rb1 exerts neuroprotective effects through regulation of Lactobacillus helveticus abundance and GABAA receptor expression

Huimin Chen,Jiajia Shen,Haofeng Li,Xiao Zheng,Dian Kang,Yangfan Xu,Chong Chen,Huimin Guo,Lin Xie,Guangji Wang,Yan Liang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.1

Background: Ginsenoside Rb1 (Rb1), one of the most abundant protopanaxadiol-type ginsenosides, exertsexcellent neuroprotective effects even though it has low intracephalic exposure. Purpose: The present study aimed to elucidate the apparent contradiction between the pharmacokineticsand pharmacodynamics of Rb1 by studying the mechanisms underlying neuroprotective effects ofRb1 based on regulation of microflora. Methods: A pseudo germ-free (PGF) rat model was established, and neuroprotective effects of Rb1 werecompared between conventional and PGF rats. The relative abundances of common probiotics werequantified to reveal the authentic probiotics that dominate in the neuroprotection of Rb1. The expressionsof the gamma-aminobutyric acid (GABA) receptors, including GABAA receptors (a2, b2, and g2) andGABAB receptors (1b and 2), in the normal, ischemia/reperfusion (I/R), and I/RþRb1 rat hippocampus andstriatum were assessed to reveal the neuroprotective mechanism of Rb1. Results: The results showed that microbiota plays a key role in neuroprotection of Rb1. The relativeabundance of Lactobacillus helveticus (Lac.H) increased 15.26 fold after pretreatment with Rb1. I/R surgeryinduced effects on infarct size, neurological deficit score, and proinflammatory cytokines (IL-1b, IL-6, andTNF-a) were prevented by colonizing the rat gastrointestinal tract with Lac.H (1 109 CFU) by gavage 15d before I/R surgery. Both Rb1 and Lac.H upregulated expression of GABA receptors in I/R rats. Coadministrationof a GABAA receptor antagonist significantly attenuated neuroprotective effects of Rb1 and Lac.H. Conclusion: In sum, Rb1 exerts neuroprotective effects by regulating Lac.H and GABA receptors ratherthan through direct distribution to the target sites.

Ginsenoside Rb1 exerts neuroprotective effects through regulation of Lactobacillus helveticus abundance and GABA_A receptor expression

Chen, Huimin,Shen, Jiajia,Li, Haofeng,Zheng, Xiao,Kang, Dian,Xu, Yangfan,Chen, Chong,Guo, Huimin,Xie, Lin,Wang, Guangji,Liang, Yan The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1

Background: Ginsenoside Rb1 (Rb1), one of the most abundant protopanaxadiol-type ginsenosides, exerts excellent neuroprotective effects even though it has low intracephalic exposure. Purpose: The present study aimed to elucidate the apparent contradiction between the pharmacokinetics and pharmacodynamics of Rb1 by studying the mechanisms underlying neuroprotective effects of Rb1 based on regulation of microflora. Methods: A pseudo germ-free (PGF) rat model was established, and neuroprotective effects of Rb1 were compared between conventional and PGF rats. The relative abundances of common probiotics were quantified to reveal the authentic probiotics that dominate in the neuroprotection of Rb1. The expressions of the gamma-aminobutyric acid (GABA) receptors, including GABAA receptors (α2, β2, and γ2) and GABAB receptors (1b and 2), in the normal, ischemia/reperfusion (I/R), and I/R+Rb1 rat hippocampus and striatum were assessed to reveal the neuroprotective mechanism of Rb1. Results: The results showed that microbiota plays a key role in neuroprotection of Rb1. The relative abundance of Lactobacillus helveticus (Lac.H) increased 15.26 fold after pretreatment with Rb1. I/R surgery induced effects on infarct size, neurological deficit score, and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) were prevented by colonizing the rat gastrointestinal tract with Lac.H (1 × 10⁹ CFU) by gavage 15 d before I/R surgery. Both Rb1 and Lac.H upregulated expression of GABA receptors in I/R rats. Coadministration of a GABA_A receptor antagonist significantly attenuated neuroprotective effects of Rb1 and Lac.H. Conclusion: In sum, Rb1 exerts neuroprotective effects by regulating Lac.H and GABA receptors rather than through direct distribution to the target sites.

In vitro differentiation of spermatogonial stem cells using testicular cells from Guangxi Bama mini-pig

Huimin Zhao,Junyu Nie,Xiangxing Zhu,Yangqing Lu,Xingwei Liang,Huiyan Xu,Xiaogan Yang,Yunkai Zhang,Kehuan Lu,Shengsheng Lu 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.5

In this study, we attempted to establish a culture system for in vitro spermatogenesis from spermatogonial stem cells (SSCs) of Bama mini-pig. Dissociated testicular cells from 1-month-old pigs were co-cultured to mimic in vivo spermatogenesis. The testicular cells were seeded in minimum essential medium alpha (a-MEM) supplemented with Knockout serum replacement (KSR). Three-dimensional colonies formed after 10 days of culture. The colonies showed positive staining for SSC-associated markers such as UCHL1, PLZF, THY1, OCT4, Dolichos biflorus agglutinin, and alkaline phosphatase. Induction of SSCs was performed in a-MEM + KSR supplemented with retinoic acid, bone morphogenetic protein 4, activin A, follicle-stimulating hormone, or testosterone. The results showed that STRA8, DMC1, PRM1, and TNP1 were upregulated significantly in the colonies after induction compared to that in testis from 1-month-old pigs, while expression levels of those genes were significantly low compared to those in 2-month-old testis. However, upregulation of ACROSIN was not significant. Replacement of a-MEM and KSR with Iscove’s modified Dulbecco’s medium and fetal bovine serum did not upregulate expression of these genes significantly. These results indicate that SSCs of Bama mini-pig could undergo differentiation and develop to a post-meiotic stage in α-MEM supplemented with KSR and induction factors.

Research on a fault diagnosis method of rolling bearings using variation mode decomposition and deep belief network

Huimin Zhao,Hailong Liu,Junjie Xu,Chen Guo,Wu Deng 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.9

The working conditions of rolling bearings during the running change in real time. Aiming at the problem of fault diagnosis of rolling bearing under complex working conditions, a new fault diagnosis (VHDBN) method based on variation mode decomposition (VMD), Hilbert transform (HT) and deep belief network (DBN) is proposed in this paper. Firstly, the proposed fault diagnosis method performs the VMD decomposition for the time domain signal in order to obtain a series of intrinsic mode functions (IMFs), and Hilbert envelope spectrum is obtained by Hilbert transform. The Hilbert envelope spectrum is used to construct the feature matrix, which is used as an input of the DBN network in order to obtain a fault diagnosis model. In order to test and verify the effectiveness of the proposed fault diagnosis method, the experimental data of rolling bearings under variable load is used in here. The experimental results show that the VMD-Hilbert envelope spectrum can better reflect the fault characteristics than the time domain spectrum, and the proposed fault diagnosis method under variable load has higher recognition accuracy than other comparison methods.

Optimization Design for Dynamic Characters of Electromagnetic Apparatus Based on Niche Sorting Multi-objective Particle Swarm Algorithm

Le Xu,Jiaxin You,Haidan Yu,Huimin Liang 한국자기학회 2016 Journal of Magnetics Vol.21 No.4

The electromagnetic apparatus plays an important role in high power electrical systems. It is of great importance to provide an effective approach for the optimization of the high power electromagnetic apparatus. However, premature convergence and few Pareto solution set of the optimization for electromagnetic apparatus always happen. This paper proposed a modified multi-objective particle swarm optimization algorithm based on the niche sorting strategy. Applying to the modified algorithm, this paper guarantee the better Pareto optimal front with an enhanced distribution. Aiming at shortcomings in the closing bounce and slow breaking velocity of electromagnetic apparatus, the multi-objective optimization model was established on the basis of the traditional optimization. Besides, by means of the improved multi-objective particle swarm optimization algorithm, this paper processed the model and obtained a series of optimized parameters (decision variables). Compared with other different classical algorithms, the modified algorithm has a satisfactory performance in the multi-objective optimization problems in the electromagnetic apparatus.

Anti-sway Controls of Double-pendulum Rotary Cranes

Xiang Xu,Huimin Ouyang 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10

Crane systems with double-pendulum effect seem more practical than those with single-pendulum effect. However, in these cases the load sway properties become more complicated so that the difficulty of the dynamic performance analysis and controller design is increased. In order to solve the aforementioned problems, the linear dynamics of a rotary crane with double-pendulum effect is derived based on disturbance observers, and is decoupled for controller design by modal analysis. Next, an S-shaped curve is generated on the basis of the decoupled linear crane model for suppressing residual double-pendulum load sway. Parameters of the trajectory can be easily obtained by calculating algebraic equations. Finally, simulation results validate the effectiveness of the proposed method.

MINDFULNESS VS. MINDLESSNESS: CLIMATE-FRIENDLY FOOD VALUE PERCEPTION AND CHOICE

Juran Kim,Huimin Xu 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07

The purpose of this study is to clarify the concept of mindfulness and examine its effects on climate-friendly food perception and choice, thereby uncovering climate-friendly food by parsing out the different effects of mindfulness vs. mindlessness and exploring the key consumer outcomes. The value of this study lies in clarifying the role of climate-friendly food and mindfulness in perceived food value and food choice. This study provides a framework for examining the effects of climate-friendly food and mindfulness, thereby shedding light on climate-friendly food and discovering perceived climate-friendly food value and food choice as main outcomes. This study contributes to the literature on value by clarifying the concept of mindfulness and the relationships between perceived food value and food choice. The findings also offer practical implications for how marketing strategies for value work more effectively and cater to different types of mindfulness.