Investigation on Ploidy Level and Reproductive Characteristic of Whipgrass

Chen Lingzhi,Yang Chunhua,Tang Zhisong,Huang Huijun,Zhang Wenjun 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

26 wild accessions and 2 cultivars of whip grass (Hemarthria compress a (L. f.) R. Br.), which collected in the fields of Sichuan Agricultural University (SAU), were used to study on their ploidy level and reproductive characteristics. The results showed that there were 4 tetraploids (14.3%), H028, H029, H052, H053, chromosome numbers were 2n=4x=36, and 24 hexaploids (85.7%), 2n=6x=54. There was no significant correlation between geographical distribution and ploidy level in this research. From 2006 to 2008, hexaploids set few seeds, but all of the tetraploids set some seeds more or less. H052 had the highest seed set (11.22%). The tetraploids had potential for seed production, especially H052, it should be possible to obtain hybrids by cross-pollination in the future.

H<SUB>∞</SUB> Filtering for Uncertain Systems with Limited Communication Capacity

Huijun Gao,Tongwen Chen 제어로봇시스템학회 2006 제어로봇시스템학회 국제학술대회 논문집 Vol.2006 No.10

Arctic Sea Ice Decline Intensified Haze Pollution in Eastern China

Wang Huijun,Huopo Chen,Jiping Liu 한국기상학회 2015 한국기상학회 학술대회 논문집 Vol.2015 No.10

A Self-Supervised Learning Framework for Under-Sampling Pattern Design Using Graph Convolution Network

Yuze Li,Huijun Chen 대한자기공명의과학회 2020 Investigative Magnetic Resonance Imaging Vol.24 No.4

Purpose: To generate the under-sampling pattern using a self-supervised learning framework based on a graph convolutional network. Materials and Methods: We first decoded the k-space data into the graph and put it into the network. After the processing of graph convolution layers and graph pooling layers, the network generated the under-sampling pattern for MR reconstruction. We trained the network on the simulated brain dataset enabled by the selfsupervised learning strategy. We did simulation along with the in vivo brain and liver experiments under different noise levels and accelerating factors to compare the performance between the proposed method and traditional methods using the PSNR and SSIM index. Results: The simulation experiments showed that the proposed method can achieve the best performance with low accelerating factors (2 and 3) at all noise levels and in high accelerating factors (4 and 5) at high noise levels (50 and 70 dB). In in vivo experiments, the proposed method attained the highest PSNR and SSIM in the brain dataset as well as in the liver dataset after fine tuning on a small liver dataset. Conclusion: The self-supervised learning framework based on a graph convolutional network was able to design the under-sampling mask for MR reconstruction. The superior performance in the simulation and in vivo experiments demonstrated the feasibility and flexibility of the proposed method and its potential in clinical use.

Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells

Qiang Fu,Huijun Shi,Yan Ren,Fei Guo,Wei Ni,Jun Qiao,Pengyan Wang,Hui Zhang,Chuangfu Chen 한국미생물학회 2014 The journal of microbiology Vol.52 No.7

Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.

The Effects of Fu Zi on Changes in the Body Heat of Dogs

Tian-Tian Chen,Chunhua Qi,Huijun Guo,Ziqiang Cheng,Dong Zhou,Haitao Liu,Jianzhu Liu 사단법인약침학회 2009 Journal of Acupuncture & Meridian Studies Vol.2 No.1

The aim of the present study was to investigate the effects of Fu Zi on changes in the body heat of dogs. Twelve clinically healthy dogs were divided into two groups: the control group (six dogs) and the experimental group (six dogs). The control group was made to ingest normal saline mixed with canned meat, while the experimental group was made to ingest the Fu Zi solution mixed with canned meat. The infrared thermographic system was used to determine the level of body heat generated by these dogs. These areas include the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr), and ventrocaudal (VCd) regions at pretreatment and were determined at 10, 20, 30, 50, 90, 120, 240, and 360 minutes after treatment for each of these areas. The results showed a tendency toward increased body heat until 30 minutes after ingestion of the Fu Zi powder mixed with canned meat. The significant differences in the changes of body heat were detected at 360 minutes in the DCd regions, 20 minutes in the VCr regions, and 30 minutes in the VCd regions between the experimental and control groups (p < 0.05). Based from our results, we find that Fu Zi can increase and maintain the dogs’ body heat for at least 6 hours. The aim of the present study was to investigate the effects of Fu Zi on changes in the body heat of dogs. Twelve clinically healthy dogs were divided into two groups: the control group (six dogs) and the experimental group (six dogs). The control group was made to ingest normal saline mixed with canned meat, while the experimental group was made to ingest the Fu Zi solution mixed with canned meat. The infrared thermographic system was used to determine the level of body heat generated by these dogs. These areas include the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr), and ventrocaudal (VCd) regions at pretreatment and were determined at 10, 20, 30, 50, 90, 120, 240, and 360 minutes after treatment for each of these areas. The results showed a tendency toward increased body heat until 30 minutes after ingestion of the Fu Zi powder mixed with canned meat. The significant differences in the changes of body heat were detected at 360 minutes in the DCd regions, 20 minutes in the VCr regions, and 30 minutes in the VCd regions between the experimental and control groups (p < 0.05). Based from our results, we find that Fu Zi can increase and maintain the dogs’ body heat for at least 6 hours.

Controlled crystallinity and crystallographic orientation of Cu nanowires fabricated in ion-track templates

Duan, Jinglai,Liu, Jie,Mo, Dan,Yao, Huijun,Maaz, Khan,Chen, Yonghui,Sun, Youmei,Hou, Mingdong,Qu, Xiaohua,Zhang, Ling,Chen, Yanfeng IOP Pub 2010 Nanotechnology Vol.21 No.36

<P>The hallmark of materials science is the ability to tailor the structures of a given material to provide a desired response. In this work, the structures involving crystallinity and crystallographic orientation of Cu nanowires electrochemically fabricated in ion-track templates have been investigated as a function of fabrication condition. Both single crystalline and polycrystalline nanowires were obtained by adjusting applied voltages and temperatures of electrochemical deposition. The anti-Hall–Petch effect was experimentally evidenced in the polycrystalline nanowires. The dominant crystallographic orientations of wires along [111], [100], or [110] directions were obtained by selecting electrochemical deposition conditions, i.e., H<SUB>2</SUB>SO<SUB>4</SUB> concentration in electrolyte, applied voltage, and electrodeposition temperature. </P>

Anionic defect engineering of transition metal oxides for oxygen reduction and evolution reactions

Zhu, Yunmin,Liu, Xi,Jin, Shiguang,Chen, Huijun,Lee, Wonyoung,Liu, Meilin,Chen, Yan The Royal Society of Chemistry 2019 Journal of materials chemistry. A, Materials for e Vol.7 No.11

<P>The development of cost-effective catalysts for the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) is crucial for enhancing the energy efficiency of many electrochemical energy conversion and storage devices. Owing to their low cost and high activity, transition metal oxides have attracted much attention as alternative electrocatalysts to replace the currently used noble metal-based catalysts. Anion defects (<I>e.g.</I>, oxygen vacancies, interstitials, and anion dopants) can significantly change the electronic structure of oxides or the stability of adsorbed intermediates, thus greatly enhancing the electrocatalytic activities of the oxide surface. Anionic defect engineering represents a potential new direction for rational design of high-performance electrocatalysts. In this review, recent progress in manipulating the anion defects in transition metal oxides for enhancing their activity and stability is summarized and the proposed mechanisms for enhanced performance are discussed in detail. Challenges and prospects are also discussed in the development of a new generation of highly efficient ORR and OER electrocatalysts.</P>

Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken

Qin, Junhui,Zhang, Hui,Bao, Huijun,Zhou, Qiang,Liu, Yi,Xu, Chunsheng,Chu, Xiaohong,Chen, Qiusheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

Cdc25B is a mitotic regulator that might act as a starter phosphatase to initiate the positive feedback loop at the entry into mitotic (M) phase. In the present study, distribution of Cdc25B mRNA in duodenal mucosa of the chicken was demonstrated by means of in situ hybridization histochemistry (ISHH) using sense and antisense digoxigenin (DIG)-labeled RNA probes. The results showed that there were many labeled cells distributing in the duodenal mucosa of the adult chicken. Of these labeled cells, 81.60${\pm}$9.63% of Cdc25B mRNA positive cells was distributed in the basilar part and mid-portion of the intestinal gland and 36.21${\pm}$8.81% in the middle and basilar portion of villi of the small intestine of the chicken, respectively. Most of these labeled cells were positive in the regions of the stem cell and proliferation. The signals of ISHH decreased from basilar to upper part in the crypt of Lieberkuhn and weakened in the inferior villi of the duodenum. Moreover, the positive signals were both in the cytoplasm and cell nucleus. However, the labeled cells were negative in both the lamina muscularis mucosae and muscular layer. The results of ISHH suggested the existence of Cdc25B mRNA and vigorous proliferation activities in the duodenal mucosa of adult chicken, replenishing the cells which had sloughed off from the superior part of the villus. Our results provide some molecular evidence for a regular pattern of avian intestinal epitheliosis and functional partition and provide an approach to further study of the locations of Cdc25B in the chicken.

Treatment of Mycoplasma wenyonii Infection in Cows with Imidocarb Dipropionate Injection-acupuncture

Jianzhu Liu,Zhengui Yan,Tiantian Chen,Ziqiang Cheng,Huijun Guo,Zhenyong Wang,Yong Wang 사단법인약침학회 2008 Journal of Acupuncture & Meridian Studies Vol.1 No.2

Sixteen shorthorn cows from Xiazhuang farm were admitted to the Veterinary Teaching Hospital at the College of Animal Science and Veterinary Medicine, Shandong Agricultural University for evaluation of poor appetite, listlessness, fever, tachycardia, tachypnea, lethargy, positive jugular venous pulse and anemia. Blood smear examination and polymerase chain reaction analysis in these cows revealed an infection with Mycoplasma wenyonii. The subjects were divided into two groups: control group (three cows) treated with intramuscular injection with imidocarb dipropionate (3 mg/kg/day for 2 days) and the experimental group (13 cows), treated with injection-acupuncture (Imidocarb Dipropionate, 1 mg/kg, once every 3 days for 6 days) at BL17, BL18, BL20, BL25, ST36, SP06 and CV04. At day 15, negative results were found using blood smear examination in all control and experimental groups. Sixteen shorthorn cows from Xiazhuang farm were admitted to the Veterinary Teaching Hospital at the College of Animal Science and Veterinary Medicine, Shandong Agricultural University for evaluation of poor appetite, listlessness, fever, tachycardia, tachypnea, lethargy, positive jugular venous pulse and anemia. Blood smear examination and polymerase chain reaction analysis in these cows revealed an infection with Mycoplasma wenyonii. The subjects were divided into two groups: control group (three cows) treated with intramuscular injection with imidocarb dipropionate (3 mg/kg/day for 2 days) and the experimental group (13 cows), treated with injection-acupuncture (Imidocarb Dipropionate, 1 mg/kg, once every 3 days for 6 days) at BL17, BL18, BL20, BL25, ST36, SP06 and CV04. At day 15, negative results were found using blood smear examination in all control and experimental groups.