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rDNA와 말단소체 반복서열 탐침을 이용한 천마의 FISH 염색체 조성 분석
Hui Chao Zhou,박응준,김현희 한국약용작물학회 2018 한국약용작물학회지 Vol.26 No.2
Background: Gastrodia elata Blume is a saprophytic perennial plant in the Orchidaceae family, because of its agricultural and medicinal effectiveness, researchers focus on its genome and chemical components. However, cytogenetic information based on the chromosome structure and composition to construct chromosomal backbone for genome sequencing research and for the development and breeding of plants is very limited. Methods and Results: We determined the metaphase chromosome composition of the G. elata genome by fluorescence in situ hybridization (FISH) using 5S and 45S rDNAs and telomeric repeat probes. The nuclear genome of G. elata was organized into 2 n = 36, with relatively small (2.71 - 5.50㎛)chromosomes that showed gradual decrease in size. Conglutination phenomenon was observed among the metaphase chromosomes, and it was distinguished from that in other plant metaphase chromosome spreads. One pair of signal was detected for each 5S and 45S rDNA in the pericentromeric region and interstitial region on the short arm of chromosomes 10 and 4, respectively, and telomeric DNA signals were detected in the terminal region of most chromosomes. Conclusions: To our knowledge, this is the first FISH chromosome composition result in G. elata and could be useful in more comprehensive molecular cytogenetic and genomic analyses as well as breeding programs of the medicinal plant G. elata.
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Hui Chao Zhou,Remnyl Joyce Pellerin,Nomar Espinosa Waminal,양태진,김현희 한국유전학회 2019 Genes & Genomics Vol.41 No.7
Background The family Araliaceae contains many medicinal species including ginseng of which the whole genome sequencing analyses have been going on these days. Objective To characterize the chromosomal distribution of 5S and 45S rDNAs and telomeric repeat in four ginseng related species of Aralia elata (Miq.) Seem., Dendropanax morbiferus H. Lév., Eleutherococcus sessiliflorus (Rupr. Et Maxim.) Seem., Kalopanax septemlobus (Thunb. ex A.Murr.) Koidz. Method Pre-labelled oligoprobe (PLOP)-fluorescence in situ hybridization (FISH) was carried out. Results The chromosome number of A. elata was 2n = 24, whereas that of the other three species of D. morbiferus, E. sessiliflorus, and K. septemlobus was 2n = 48, corresponding to diploid and tetraploid, respectively, based on the basic chromosome number x = 12 in Araliaceae. In all four species, one pair of 5S signals were detected in the proximal regions of the short arms of chromosome 3, whereas in K. septemlobus, the 5S rDNA signals localized in the subtelomeric region of short arm of chromosome 3, while all the 45S rDNA signals localized at the paracentromeric region of the short arm of chromosome 1. And the telomeric repeat signals were detected at the telomeric region of both short and long arms of most chromosomes. Conclusion The PLOP-FISH was very effective compared with conventional FISH method. These results provide useful comparative cytogenetic information to better understand the genome structure of each species and will be useful to trace the history of ginseng genomic constitution.
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In silico mining and FISH mapping of a chromosome‑specific satellite DNA in Capsicum annuum L.
Hui Chao Zhou,Nomar Espinosa Waminal,김현희 한국유전학회 2019 Genes & Genomics Vol.41 No.9
Background A large proportion of eukaryote nuclear genomes is composed of repetitive DNA. Tracing the dynamics of repetitive elements in the genomes of related taxa can reveal important information about their phylogenic relationships as well as traits that have become distinct to a lineage. Objective Study the genomic abundance and chromosomal location of repetitive DNA in Capsicum annuum L. to understand the repeat dynamics. Method We quantified repeated DNA content in the C. annuum genome using the RepeatExplorer pipeline. Results About 42% of the C. annuum genome dataset comprised repetitive elements. Of these, 0.011, 0.98, 3.09, and 0.024% represented high and low confidence satellite repeats, putative long-terminal repeats (LTRs), and rDNA sequences, respectively. One novel high confidence 167-bp satellite repeat with a genomic proportion of 0.011%, Ca167TR, was identified. Furthermore, FISH with Ca167TR on metaphase chromosomes of C. annuum revealed signals in the subtelomeric regions of the short and long arms of chromosome 3 and 4, respectively. Conclusion Further understanding of the origin and associated functions of Ca167TR and other repeats in Capsicum will give us insights into the genomic relationships and functions of the genome.
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Long-term Prognosis in Hepatocellular Carcinoma Patients after Hepatectomy
Zhou, Lei,Liu, Chang,Meng, Fan-Di,Qu, Kai,Tian, Feng,Tai, Ming-Hui,Wei, Ji-Chao,Wang, Rui-Tao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2
Background: The hepatocellular carcinoma is very common in China. Our aim in this report was to investigate clinical and pathological factors based on the current decade data that could influence prognosis of HCC patients after hepatectomy. Methods: Between 2002 and 2009, all patients undergoing hepatectomy for HCC were followed up and reviewed retrospectively. Prognostic factors were studied by univariate and multivariate analysis, with Kaplan-Meier and Cox multivariate survival analyses. Results: Complete clinicopathologic and follow-up data were available for 114 patients. The estimated cumulative survival rates at 1, 3, and 5 yr were 84.6%, 60.2% and 51.8%, respectively. On univariate analysis, key prognostic factors were AFP level, GGT level, tumor size, number of tumors, portal vein invasion, liver cirrhosis status and TNM stage. In the multivariate analysis, tumor size, GGT level, liver cirrhosis status and portal vein invasion were significantly associated with patients' prognosis. Conclusion: Through follow-up of a relatively large cohort of Chinese patients, tumor size, GGT level, liver cirrhosis status, portal vein invasion were revealed as important factors for long-term survival after hepatectomy. Early diagnosis for tumor and the improvement of liver function before surgery are important ways to improve the prognosis.
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Hui Liu,Xiu-wen Lv,Shi-chao Chen,Qiang Zhou,Piao Zhou,Wei-lian Qu 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.1
Welding defects are unavoidable for welded structures, which can lead to fatigue damage even under the random wind load with small amplitude. It is therefore necessary to explore the effect of welding defect on the fatigue properties of welded steel pipes. Three groups of welded steel pipe specimens were designed according to welding defect conditions, i.e. specimens without welding defect (Group I), specimens with incomplete fusion (Group II), and specimens with welding porosity (Group III). Uniaxial tension–compression and torsion high-cycle fatigue tests were carried out. S–N curves of uniaxial tension–compression and torsion tests were obtained by cyclic loading with equal stress amplitude. The test results show that the high-cycle fatigue strength of weldments is obviously lower than that of base metal with the same strength under uniaxial tension–compression and torsion loading. In addition, the welding defects result in a decrease in fatigue strength, while the decrease extent by welding porosity is greater than that by incomplete fusion. Finally, because of the inherent multiaxial loading characteristics of welded structures, the high-cycle multiaxial fatigue life of steel pipe weldments was also predicted by using the modified Wöhler curve method based on the uniaxial fatigue test results. It can be found that when the stress amplitude is constant, the fatigue life of welded steel pipe decreases and the modified Wöhler curves move downward more quickly with the increase of damage parameter defined as the ratio of normal stress amplitude to shear stress amplitude on the critical plane, which means that normal stress amplitude will accelerate the cracks growth and result in faster failure of the weld materials.
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Hui-wen Xiao,Yuan Li,Dan Luo,Jia-li Dong,Li-xin Zhou,Shu-yi Zhao,Qi-sheng Zheng,Hai-chao Wang,Ming Cui,Sai-jun Fan 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
Although radiation therapy is a cornerstone of modern management of malignancies, various side effects are inevitably linked to abdominal and pelvic cancer after radiotherapy. Radiation-mediated gastrointestinal (GI) toxicity impairs the life quality of cancer survivors and even shortens their lifespan. Hydrogen has been shown to protect against tissue injuries caused by oxidative stress and excessive inflammation, but its effect on radiation-induced intestinal injury was previously unknown. In the present study, we found that oral gavage with hydrogen-water increased the survival rate and body weight of mice exposed to total abdominal irradiation (TAI); oral gavage with hydrogen-water was also associated with an improvement in GI tract function and the epithelial integrity of the small intestine. Mechanistically, microarray analysis revealed that hydrogen-water administration upregulated miR-1968-5p levels, thus resulting in parallel downregulation of MyD88 expression in the small intestine after TAI exposure. Additionally, high-throughput sequencing showed that hydrogen-water oral gavage resulted in retention of the TAI-shifted intestinal bacterial composition in mice. Collectively, our findings suggested that hydrogen-water might be used as a potential therapeutic to alleviate intestinal injury induced by radiotherapy for abdominal and pelvic cancer in preclinical settings.
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Hui Tong,Chao Yang,Yanqi Lv,Ling Wang,Koucheng Chen,Xingfu Zhou 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.96 No.-
In this study, tubular titanium membrane (TTM) /SnO2-Sb/SnO2-Sb-CeO2 porous anode was fabricatedand used in continuous tubular membrane reactor for electrochemical treatment of dye wastewater. Xraydiffraction (XRD), scanning electron microscopy (SEM) and high-resolution transmission electronmicroscopy (HRTEM) were used to evaluate the morphology and microstructure of the different tubulartitanium membrane. Linear sweep voltammetry (LSV) and accelerated service life test are employed toillustrate the performance of TTM/SnO2-Sb/SnO2-Sb-CeO2 porous membrane electrode. It is found thatTTM/SnO2-Sb/SnO2-Sb-CeO2 active layer on titanium membrane has compact microstructure, high over-potential for oxygen evolution (2.10 V vs saturated calomel electrode). The effects of pore diameter,applied voltage andflow rate on the electro-catalytic property of the tubular porous electrode wereinvestigated. Study shows titanium membrane reactor with an optimized pore diameter of 10 mm has thelowest energy consumption which is important for the practical application of electrochemicaltechnology. The performance of titanium tubular membrane reactor is investigated by treatingmethylene blue (MB) wastewater under the cell voltage of 3.0–4.5 V and theflow rate of 2.5–3.5 L min 1. This continuous titanium membrane electrochemical reactor using solar cell show excellent performancein treating dye wastewater and the further potential application in electrochemical synthesis.
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( Jin Zhou Zhu ),( Yu Ming Wang ),( Chao Hui Yu ),( You Ming Li ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Fatty liver is a growing health issue in China. However, the research concerning the infi uence of economic development on the prevalence of fatty liver was sparse. This study is to investigate the relation between economic status and the adult prevalence of fatty liver in the mainland of China. Methods: Literature searches on PubMed, EMBASE, Web of Science and Chinese National Knowledge Infrastructure databases were performed to identify eligible studies published before July 10, 2014. Gross domestic product (GDP) per capita was chosen to assess the economic status. The correlations were obtained by Pearson coeffi cient. Results: 27 studies, from 22 cities and 2 provinces from 1995 to 2013, were included in this study. In general, the prevalence of fatty liver presented a signifi cant correlation with GDP per capita (r = 0. 459, P = 0. 016). To be specifi c, the prevalence in north China correlated with GDP per capita (r = 0. 879, P = 0. 05) and survey year (r = 0. 909, P = 0. 032). In south China, on the contrary, the prevalence failed to correlate with the two indicators. According to the data from multiple years, the city of Shanghai witnessed an ascending prevalence. In terms of gender, the prevalence of female correlated with GDP per capita (r = 0. 572, P = 0. 004) and survey year (r = 0. 483, P = 0. 020). Meanwhile, there was no correlation between the prevalence of male and GDP per capita (P = 0. 129) or survey year (P = 0. 358). Conclusions: In the mainland of China, higher GDP per capita suggested higher adult prevalence of fatty liver, based on the data in the past 20 years. With the development of economy, public health interventions, thus, are essential to prevent the trend of obesity and alcohol abuse.
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Hadassah Roa Belandres,Hui Chao Zhou,Nomar Espinosa Waminal,이수영,허진회,김현희 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.2
×Brassicoraphanus (AARR, 2n = 38) is a synthetic intergeneric allopolyploid between Brassica rapa L. ssp. pekinensis (AA, 2n = 20) and Raphanus sativus L. var. rafiphera (RR, 2n = 18). Abnormalities in meiosis are main causes for infertility, especially in recent intergeneric allopolyploids. Several ×Brassicoraphanus lines showing varied fertility rates were produced previously, but no cytogenetic data specifying the reasons for infertility have been reported. In this study, we performed cytogenetic analyses in BB4, BB6, BB12, and BB50 lines to evaluate their chromosomal composition and behavior during meiosis. The four lines had relatively small chromosomes, ranging in length from 1.82 to 5.72 mm. BB6, BB12, and BB50 have euploid chromosome number of 2n = 38, whereas BB4 is an aneuploid with 2n ‒ 1 = 37. Fluorescent in situ hybridization karyotype analysis by using 5S/45S rDNA revealed 5/7, 6/7, 5/5 and 5/5 pairs in BB4, BB6, BB12 and BB50, respectively. Genomic in situ hybridization analysis on cells in prophase I revealed varying frequencies of tetravalent pairing and sticky, ring, rod, and laggard chromosomes across the lines, which were more abundant in BB4 and BB6. Unlike BB4 and BB6, both BB12 and BB50 are known to have relatively higher seed fertility and uniform plant morphology. The varied degrees of chromosomal pairing stability during meiosis could explain the different fertility rates among the four ×Brassicoraphanus lines in this study. These data might facilitate breeding programs of ×Brassicoraphanus and further cytogenomic analyses.
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