Effect of dietary supplementation of Bacillus subtilis TLRI 211-1 on laying performance, egg quality and blood characteristics of Leghorn layers

Tsai Ming-Yang,Shih Bor-Ling,Liaw Ren-Bao,Chen Wen-Tsen,Lee Tsung-Yu,Hung Hsi-Wen,Hung Kuo-Hsiang,Lin Yih-Fwu 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.4

Objective: TLRI 211-1 is a novel Bacillus subtilis strain. This experiment was to investigate dietary supplementation of TLRI 211-1 on laying performance, egg quality and blood characteristics of layers. Methods: One hundred and twenty 65-wk-old Leghorn layers were divided into four treatment groups for 8 weeks experiment. Each treatment had three replicates. The basal diet was formulated as control group with crude protein 17% and metabolizable energy 2,850 kcal/kg and supplemented with TLRI 211-1 0.1%, 0.3%, and commercial Bacillus amyloliquefaciens 0.1% as treatment 2, 3 and 4 groups, respectively. Both TLRI 211-1 and commercial Bacillus amyloliquefaciens were adjusted to contain 1×109 colony-forming unit (CFU)/mL (g), hence the 0.1% supplemental level was 1×109 CFU/kg. Results: The results showed that TLRI 211-1 0.3% and commercial B. amyloliquefaciens groups had higher weight gain than the other groups; TLRI 211-1 0.1% group had better feed to eggs conversion ratio than the control and commercial B. amyloliquefaciens groups (p<0.05). Bacillus subtilis supplementation increased yolk weight (p<0.05). In egg quality during storage, TLRI 211-1 0.1% had higher breaking strength than the control group at the second week of storage (p<0.05). At the third week of storage, TLRI 211-1 0.3% had higher Haugh unit (p<0.05). Hens fed diets supplemented with TLRI 211-1 0.3% significantly decreased blood triglyceride levels and increased blood calcium levels (p< 0.05). TLRI 211-1 0.3% group had lower H2S (p<0.05) and hence had less unpleasant odor in excreta of hens. Conclusion: In conclusion, supplementation with 0.1% TLRI 211-1 can significantly improve feed to eggs conversion ratio. TLRI 211-1 supplementation also can maintain eggs at their optimum quality level during storage. The study showed that B. subtilis TLRI 211-1 can be used as feed additives for improving egg production performance and egg quality.

Rapid screening of roundup ready soybean in food samples by a hand-held PCR device

Hsiang-Yun Tung,Sue-Hong Wang,Yu-Cheng Chiang,Ming-Shiun Tsai 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

Insulated isothermal PCR (iiPCR) method was recently available for rapid on-site detection ofroundup ready soybean (RRS; event GTS40-3-2) in food materials and products. Performance of thismethod was evaluated in this study. The 100% detection endpoint for the RRS by iiPCR was found insamples containing 0.1% RRS, equivalent to the results of the reference real-time PCR (rtPCR). Analysisof nucleic acids of soybean-based processed food products indicated 95% agreement between the iiPCRand rtPCR for RRS detection. By testing soybean milk and tofu samples using simple pretreatmentmethods, we found that the agreements between iiPCR and rtPCR methods of the aforementionedsamples were 80% and 90%, respectively. Replicated tests of all discrepant samples implied that thesesamples had trace amounts of RRS, suggesting that the iiPCR system is more sensitive than the rtPCRmethod. In conclusion, the iiPCR technology can be a useful point-of-need tool to help make a timelydecision in the consumption of genetically modified organisms.

Credibility Judgment Predictors for Child Sexual Abuse Reports in Forensic Psychiatric Evaluations

Ling-Hsiang Wang,Yu-Yung Hung,Philip C,Chow,Che-Sheng Chu,Hsing-Jung Li,Ti Lu,Ching-Hong Tsai 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.2

Objective We intended to analyze the credibility judgment in written forensic psychiatric reports of child sexual abuse registered in Southern Taiwan. Methods Ninety-six cases of child sexual abuse between August 2010 and October 2017 encountered in two hospitals were analyzed. The results in these reports were categorized into credible and non-credible. We identified the factors that distinguished between the two groups in bivariate analyses using chi-square test. A binary logistic regression analysis was performed to determine whether the factors that significantly correlated in the bivariate analyses were independent predictors of credible judgments. Results Among 96 cases, 70 (73%) were judged as credible. Consistent testimonies of children (odds ratio=40.82) and multiple abuse events (odds ratio=6.05) were positive variables independently related to the sexual abuse allegations judged as credible. Conclusion The number of allegations judged as credible in this study was slightly higher than that reported in other studies. Our findings about predictors for credible cases are not in line with those reported previously. Due to the differences in resources of the cases and backgrounds of the evaluators among multiple studies, direct comparisons with previous studies must be treated with caution.

Rapid screening of roundup ready soybean in food samples by a hand-held PCR device

Tung, Hsiang-Yun,Wang, Sue-Hong,Chiang, Yu-Cheng,Tsai, Ming-Shiun 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

Insulated isothermal PCR (iiPCR) method was recently available for rapid on-site detection of roundup ready soybean (RRS; event GTS40-3-2) in food materials and products. Performance of this method was evaluated in this study. The 100% detection endpoint for the RRS by iiPCR was found in samples containing 0.1% RRS, equivalent to the results of the reference real-time PCR (rtPCR). Analysis of nucleic acids of soybean-based processed food products indicated 95% agreement between the iiPCR and rtPCR for RRS detection. By testing soybean milk and tofu samples using simple pretreatment methods, we found that the agreements between iiPCR and rtPCR methods of the aforementioned samples were 80% and 90%, respectively. Replicated tests of all discrepant samples implied that these samples had trace amounts of RRS, suggesting that the iiPCR system is more sensitive than the rtPCR method. In conclusion, the iiPCR technology can be a useful point-of-need tool to help make a timely decision in the consumption of genetically modified organisms.

Preparation and characterization of superhydrophobic coatings

Hsing-I Hsiang,Ming-Tsai Liang,Yu-Lun Chang,Hung-Chin Huang,Fu-Su Yen 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.3

To obtain a surface with water-repelling and self-cleaning abilities attracts much interest in the nanotechnology age. In this study, a perfluroalkyl surfactant (perflurodecanoic acid, PFDA) was coated on nano-sized boehmite surfaces to obtain hydrophobic nano-particles via surface modification. Then the hydrophobic boehmite was coated on a transparent plastic film using spin-coating to obtain a hydrophobic film. The effects of various amounts of hydrophobic polymer on the adsorption behavior and contact angle for hydrophobic properties were investigated using FTIR, and a contact-angle meter. The adsorption of PFDA onto the surface of boehmite takes place through ionic bonding. The contact angles of the thin film prepared by spin coating PFDA-modified-boehmite on glass substrates were above 150o as the adsorption of PFDA onto the surface of boehmite reached saturation.

Thermal Process for Ablation of Au films Using Femtosecond Laser with Low Laser Fluence

Ching-Yen Ho,Yu-Hsiang Tsai,Mao-Yu Wen 한국자기학회 2010 한국자기학회 학술연구발표회 논문개요집 Vol.2010 No.12

Oxygen-loaded microbubble-mediated sonoperfusion and oxygenation for neuroprotection after ischemic stroke reperfusion

Yi‑Ju Ho,Hsiang‑Lung Cheng,Lun‑De Liao,Yu‑Chun Lin,Hong‑Chieh Tsai,Chih‑Kuang Yeh 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Ischemic stroke-reperfusion (S/R) injury is a crucial issue in the protection of brain function after thrombolysis. The vasodilation induced by ultrasound (US)-stimulated microbubble cavitation has been applied to reduce S/R injury through sonoperfusion. The present study uses oxygen-loaded microbubbles (OMBs) with US stimulation to provide sonoperfusion and local oxygen therapy for the reduction of brain infarct size and neuroprotection after S/R. Methods The murine S/R model was established by photodynamic thrombosis and thrombolysis at the remote branch of the anterior cerebral artery. In vivo blood flow, partial oxygen pressure ( pO2), and brain infarct staining were examined to analyze the validity of the animal model and OMB treatment results. The animal behaviors and measurement of the brain infarct area were used to evaluate long-term recovery of brain function. Results The percentage of blood flow was 45 ± 3%, 70 ± 3%, and 86 ± 2% after 60 min stroke, 20 min reperfusion, and 10 min OMB treatment, respectively, demonstrating sonoperfusion, and the corresponding pO2 level was 60 ± 1%, 76 ± 2%, and 79 ± 4%, showing reoxygenation. After 14 days of treatment, a 87 ± 3% reduction in brain infarction and recovery of limb coordination were observed in S/R mice. The expression of NF-κB, HIF-1α, IL-1β, and MMP-9 was inhibited and that of eNOS, BDNF, Bcl2, and IL-10 was enhanced, indicating activation of anti-inflammatory and anti-apoptosis responses and neuroprotection. Our study demonstrated that OMB treatment combines the beneficial effects of sonoperfusion and local oxygen therapy to reduce brain infarction and activate neuroprotection to prevent S/R injury.

Composite electrolyte pastes for preparing sub-module dye sensitized solar cells

Shanmuganathan Venkatesan,Yun-Yu Chen,Chung-Yu Chien,Ming-Hsiang Tsai,Hsisheng Teng,Yuh-Lang Lee 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

In this study, high-efficiency sub-module quasi-solid-state (QS) dye-sensitized solar cells (DSSCs) are preparedusing silicon dioxide (SiO2) nanofiller and polyethylene oxide (PEO)-based iodide composite electrolytepastes. The influence of various amounts of nanofiller on the ionic conductivity, ion diffusioncoefficient, and the performance of the devices are explored. The addition of SiO2 decreases the ion transportproperties in the plain polymer electrolyte pastes. X-ray photoelectron spectroscopy analysis confirmsthe adsorption of polyiodide and lithium ions on the surface of the SiO2. This effect causes areduction in the concentration of ions near the photoelectrode. As a result, the QS-DSSCs using 1 wt.%SiO2 nanofillers have higher recombination resistance and a longer electron lifetime than the otherDSSCs. Therefore, high open-circuit voltage (0.794 V) and high efficiency (8.83%) are obtained for the correspondingQS-DSSCs. This efficiency is much better than the efficiencies of the devices employing thepristine polymer electrolyte paste and liquid electrolytes. This composite electrolyte paste is employedto prepare QS-sub-module cells. The four strip and rectangular-shaped module cells have high efficienciesof 4.71% and 4.81%, respectively. QS-DSSCs using 1 wt.% SiO2 nanofillers show high stability at bothroom temperature and 60 C.

Pilot-scale study on dehydration of synthetic and lignocellulosic ethanol by NaA membrane

Hwa-Jou Wei,Yu-Hsiang Weng,Tsung-Yen Tsai 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.4

A pilot scale vapor permeation unit was assembled to study the dehydration of bioethanols with NaA membrane. The operational parameters, such as feed concentration, temperature, and downstream pressure in permeate, were varied. Long-term operation of an ethanol dehydration unit was demonstrated. Additionally, the lingocellulosic ethanol distillate was dehydrated to fuel grade ethanol with less than 1% water content. The changes in impurities, such as methanol, ethylacetate, 2-propanol, 1-propanol, 1-butanol, and Isoamyl alcohol, were dependent on the volatility of the compounds. The ethanol concentrations in the permeate stream were lower than 1%, indicating high separation performance of the membrane system.

Research on the Structure and Application of Fuzzy Environmental Impact Assessment Model

( Shiaw Wen Tien ),( Chia Hsiang Hsieh ),( Yi Chan Chung ),( Chih Hung Tsai ),( Yih Huei Yu ) 한국품질경영학회 2004 The Asian Journal on Quality Vol.5 No.2