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Rapid screening of roundup ready soybean in food samples by a hand-held PCR device
Tung, Hsiang-Yun,Wang, Sue-Hong,Chiang, Yu-Cheng,Tsai, Ming-Shiun 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
Insulated isothermal PCR (iiPCR) method was recently available for rapid on-site detection of roundup ready soybean (RRS; event GTS40-3-2) in food materials and products. Performance of this method was evaluated in this study. The 100% detection endpoint for the RRS by iiPCR was found in samples containing 0.1% RRS, equivalent to the results of the reference real-time PCR (rtPCR). Analysis of nucleic acids of soybean-based processed food products indicated 95% agreement between the iiPCR and rtPCR for RRS detection. By testing soybean milk and tofu samples using simple pretreatment methods, we found that the agreements between iiPCR and rtPCR methods of the aforementioned samples were 80% and 90%, respectively. Replicated tests of all discrepant samples implied that these samples had trace amounts of RRS, suggesting that the iiPCR system is more sensitive than the rtPCR method. In conclusion, the iiPCR technology can be a useful point-of-need tool to help make a timely decision in the consumption of genetically modified organisms.
Rapid screening of roundup ready soybean in food samples by a hand-held PCR device
Hsiang-Yun Tung,Sue-Hong Wang,Yu-Cheng Chiang,Ming-Shiun Tsai 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
Insulated isothermal PCR (iiPCR) method was recently available for rapid on-site detection ofroundup ready soybean (RRS; event GTS40-3-2) in food materials and products. Performance of thismethod was evaluated in this study. The 100% detection endpoint for the RRS by iiPCR was found insamples containing 0.1% RRS, equivalent to the results of the reference real-time PCR (rtPCR). Analysisof nucleic acids of soybean-based processed food products indicated 95% agreement between the iiPCRand rtPCR for RRS detection. By testing soybean milk and tofu samples using simple pretreatmentmethods, we found that the agreements between iiPCR and rtPCR methods of the aforementionedsamples were 80% and 90%, respectively. Replicated tests of all discrepant samples implied that thesesamples had trace amounts of RRS, suggesting that the iiPCR system is more sensitive than the rtPCRmethod. In conclusion, the iiPCR technology can be a useful point-of-need tool to help make a timelydecision in the consumption of genetically modified organisms.
Ming-Shiun Tsai,Chia-Chih Chien,Ting-Hui Lin,Chia-Chi Liu,Rosa Huang Liu,Hong-Lin Su,Yung-Tsung Chiu,Sue-Hong Wang 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.11
Acetaminophen (APAP) overdose causes severe liver and kidney damage. APAP-induced liver injury (AILI) represents the most frequent cause of drug-induced liver failure. APAP is relatively insoluble and can only be taken orally; however, its prodrug, propacetamol, is water soluble and usually injected directly. In this study, we examined the timedependent effects of AILI after propacetamol injection in mice. After analyses of alanine aminotransferase and aspartate aminotransferase activities and liver histopathology, we demonstrated that a novel AILI mouse model can be established by single propacetamol injection. Furthermore, we compared the protective and therapeutic effects of galangin with a known liver protective extract, silymarin, and the only clinical agent for treating APAP toxicity, N-acetylcysteine (NAC), at the same dose in the model mice. We observed that galangin and silymarin were more effective than NAC for protecting against AILI. However, only NAC greatly improved both the survival time and rate consequent to a lethal dose of propacetamol. To decipher the hepatic protective mechanism(s) of galangin, galangin pretreatment significantly decreased the hepatic oxidative stress, increased hepatic glutathione level, and decreased hepatic microsomal CYP2E1 levels induced by propacetamol injection. In addition, propacetamol injection also reproduced the probability of APAP-induced kidney injury (AIKI), appearing similar to a clinical APAP overdose. Only galangin pretreatment showed the protective effect of AIKI. Thus, we have established a novel mouse model for AILI and AIKI using a single propacetamol injection. We also demonstrated that galangin provides significant protection against AILI and AIKI in this mouse model.